LOCUS       NZ_CP070117            24413 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1298 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP070117
VERSION     NZ_CP070117.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357440
            Assembly: GCF_016904455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24413)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24413)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 963.3x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:37:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,299
            CDSs (total)                      :: 5,180
            Genes (coding)                    :: 4,969
            CDSs (with protein)               :: 4,969
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 78 of 211
            Pseudo Genes (incomplete)         :: 128 of 211
            Pseudo Genes (internal stop)      :: 48 of 211
            Pseudo Genes (multiple problems)  :: 40 of 211
            Pseudo Genes (short protein)      :: 4 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1298"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1298"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            323..595
                     /locus_tag="I6K28_RS25490"
                     /old_locus_tag="I6K28_25435"
     CDS             323..595
                     /locus_tag="I6K28_RS25490"
                     /old_locus_tag="I6K28_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001272119.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIEVMQEQAVKAFVSWMTNQEGT
                     EFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
                     /name="hypothetical protein"
     gene            669..968
                     /locus_tag="I6K28_RS25495"
                     /old_locus_tag="I6K28_25440"
     CDS             669..968
                     /locus_tag="I6K28_RS25495"
                     /old_locus_tag="I6K28_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000113288.1"
                     /translation="MARLPYIESTTQQIFIFNTLTLCTIGLIHIETGEKYIVVFTDNNK
                     IRDYKNGVIEELGEITQEDIELIEYYAKKYEKYFDDLKIGEEPLNLKEYIECMQ"
                     /name="hypothetical protein"
     gene            1339..1872
                     /locus_tag="I6K28_RS25500"
                     /old_locus_tag="I6K28_25445"
     CDS             1339..1872
                     /locus_tag="I6K28_RS25500"
                     /old_locus_tag="I6K28_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025389.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMMK
                     AVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIWIS
                     GNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQAGG
                     WQQVEGRARVEARA"
                     /name="J domain-containing protein"
     gene            2000..2542
                     /locus_tag="I6K28_RS25505"
                     /old_locus_tag="I6K28_25450"
     CDS             2000..2542
                     /locus_tag="I6K28_RS25505"
                     /old_locus_tag="I6K28_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000206647.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRKY
                     SIIIRLERERFFIVGSVDSLVWHGNPSVCTVSLTRNPVGIANDISRKILANALDEIELL
                     REHESALQEKQDKVRIIKGMLSQLLSISPWYGALTGFNADNGLAGKILQNGDDYEMVIR
                     GLNVDQLIKVAGFIKKL"
                     /name="hypothetical protein"
     gene            2578..2835
                     /locus_tag="I6K28_RS25510"
                     /old_locus_tag="I6K28_25455"
     CDS             2578..2835
                     /locus_tag="I6K28_RS25510"
                     /old_locus_tag="I6K28_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000845367.1"
                     /translation="MKTDNVFFKSKGSYSYVVTVDMDNQMYMVRKYVKTYVKSCDKEQV
                     KGKKTVKKLVLDTNLTLPELLDLDNGILADLFTRGNNNAN"
                     /name="hypothetical protein"
     gene            2825..3070
                     /locus_tag="I6K28_RS25515"
                     /old_locus_tag="I6K28_25460"
     CDS             2825..3070
                     /locus_tag="I6K28_RS25515"
                     /old_locus_tag="I6K28_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001193619.1"
                     /translation="MQTKPTPADVKEARISAGLTLKEAAEIFGYQLNTWQMKESAGKAT
                     RSLSVGEYNYLLLLAGKHPEYVLCKRDTITAEPTTV"
                     /name="hypothetical protein"
     gene            complement(3101..3400)
                     /locus_tag="I6K28_RS25520"
                     /old_locus_tag="I6K28_25465"
     CDS             complement(3101..3400)
                     /locus_tag="I6K28_RS25520"
                     /old_locus_tag="I6K28_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001259443.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQ
                     VADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGKRVAFNI"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3397..3747)
                     /locus_tag="I6K28_RS25525"
                     /old_locus_tag="I6K28_25470"
     CDS             complement(3397..3747)
                     /locus_tag="I6K28_RS25525"
                     /old_locus_tag="I6K28_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269926.1"
                     /translation="MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHAD
                     TVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAIILCAGDKSNDKKFYDRMIRIADEE
                     FSTHLAELEGKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4113..4760
                     /locus_tag="I6K28_RS25530"
                     /old_locus_tag="I6K28_25475"
     CDS             4113..4760
                     /locus_tag="I6K28_RS25530"
                     /old_locus_tag="I6K28_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000214917.1"
                     /translation="MTVAIALVNGKGGVKKSSMSRAIATEYTKADWKVLGLDMDVDQGS
                     FLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVIDSAAYATKSLLSLAEIV
                     DLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVSESESEYRTALEYLKPCG
                     VQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQGVIDLLTKLSQ"
                     /name="ParA family protein"
     gene            4800..5033
                     /locus_tag="I6K28_RS25535"
                     /old_locus_tag="I6K28_25480"
     CDS             4800..5033
                     /locus_tag="I6K28_RS25535"
                     /old_locus_tag="I6K28_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000024515.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVDM
                     EVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(5065..5328)
                     /locus_tag="I6K28_RS25540"
                     /old_locus_tag="I6K28_25485"
     CDS             complement(5065..5328)
                     /locus_tag="I6K28_RS25540"
                     /old_locus_tag="I6K28_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001312455.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPDNDLAKITKSLRTAENRMVCVIQD
                     NNRILRWDRSYASRAKNHWRKVAPDRFEILGTVEHIHYVGKC"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(<5507..5812)
                     /locus_tag="I6K28_RS26615"
                     /pseudo=""
     CDS             complement(<5507..5812)
                     /locus_tag="I6K28_RS26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5822..6286)
                     /locus_tag="I6K28_RS25550"
                     /old_locus_tag="I6K28_25495"
     CDS             complement(5822..6286)
                     /locus_tag="I6K28_RS25550"
                     /old_locus_tag="I6K28_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_000697615.1"
                     /translation="MKILIIFALYILSPSLYANAADIQGKVIRVLDGDTIEILQNKTPV
                     RIRLANIDAPEKKQAYGRWSTDQLKSLIAGQSVRVTYSHNDRYGRIIGRVFTTGGSEAN
                     RFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHK"
                     /name="thermonuclease family protein"
     gene            complement(6290..7024)
                     /gene="mobC"
                     /locus_tag="I6K28_RS25555"
                     /old_locus_tag="I6K28_25500"
     CDS             complement(6290..7024)
                     /gene="mobC"
                     /locus_tag="I6K28_RS25555"
                     /old_locus_tag="I6K28_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_000905147.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKKS
                     RNPVYKLLNKALKEGFIIKHEIPSSKRKLTVWGITMQGLAMVVKADDITFPSYFEPSKL
                     KYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAIMAI
                     ETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVMRNNT
                     PVPFDAKNREMFIFRTIDELEQ"
                     /name="mobC"
     gene            complement(7034..8761)
                     /locus_tag="I6K28_RS25560"
                     /old_locus_tag="I6K28_25505"
     CDS             complement(7034..8761)
                     /locus_tag="I6K28_RS25560"
                     /old_locus_tag="I6K28_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_001068080.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNLP
                     REERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITILF
                     TLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPLDYI
                     DLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGEAVFV
                     EDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAGFSLSK
                     KGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELEEIALVN
                     SVNAKNGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINTTPRVV
                     AIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSVIGAVIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHNNNDVPSSGL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(9571..9876)
                     /locus_tag="I6K28_RS25565"
                     /old_locus_tag="I6K28_25510"
     CDS             complement(9571..9876)
                     /locus_tag="I6K28_RS25565"
                     /old_locus_tag="I6K28_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000910475.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQA
                     RLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAAIKAEDECLTEITRAQV"
                     /name="hypothetical protein"
     gene            complement(9904..10119)
                     /locus_tag="I6K28_RS25570"
                     /old_locus_tag="I6K28_25515"
     CDS             complement(9904..10119)
                     /locus_tag="I6K28_RS25570"
                     /old_locus_tag="I6K28_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001090643.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVCSIPSGMKGNFITSIGETLE
                     DLKKRYPDIILITESEALALQSKREN"
                     /name="hypothetical protein"
     gene            complement(10122..10421)
                     /locus_tag="I6K28_RS25575"
                     /old_locus_tag="I6K28_25520"
     CDS             complement(10122..10421)
                     /locus_tag="I6K28_RS25575"
                     /old_locus_tag="I6K28_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000716758.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGEC
                     RSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(10450..10848)
                     /locus_tag="I6K28_RS25580"
                     /old_locus_tag="I6K28_25525"
     CDS             complement(10450..10848)
                     /locus_tag="I6K28_RS25580"
                     /old_locus_tag="I6K28_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_021533590.1"
                     /translation="MIKHILVTVLVTLITGCSSPPPPVPVEWNKKPVSLNEKLPEWNPN
                     SLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSAKNWLY
                     HHGANGVIIYHPITNCLTCNETTIYFSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(10845..11870)
                     /gene="virB11"
                     /locus_tag="I6K28_RS25585"
                     /old_locus_tag="I6K28_25530"
     CDS             complement(10845..11870)
                     /gene="virB11"
                     /locus_tag="I6K28_RS25585"
                     /old_locus_tag="I6K28_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000125556.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELWTKIDGVWG
                     QHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTISIT
                     IRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSDEKGS
                     VVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHEAIDGF
                     ITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNVRRMNDIYFRPLHRNQYYEEMK
                     V"
                     /name="virB11"
     gene            complement(11860..12051)
                     /locus_tag="I6K28_RS25590"
                     /old_locus_tag="I6K28_25535"
     CDS             complement(11860..12051)
                     /locus_tag="I6K28_RS25590"
                     /old_locus_tag="I6K28_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000995652.1"
                     /translation="MNCKDKETWLHMLAAGHMGGLAGNPNEAAAQDAYQRLIKNKTAQA
                     VLQRCTTRITLKGANDDR"
                     /name="hypothetical protein"
     gene            complement(12035..13300)
                     /gene="virB10"
                     /locus_tag="I6K28_RS25595"
                     /old_locus_tag="I6K28_25540"
     CDS             complement(12035..13300)
                     /gene="virB10"
                     /locus_tag="I6K28_RS25595"
                     /old_locus_tag="I6K28_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000129037.1"
                     /translation="MTDENKSPSTEEEKPVMTSDQDDIATLEREARAKREAELQASQDD
                     EKENSLQPAVNKLKKRQKGKAAAFLAICAVALIGLTWGGNWVYQNFLKQENGEKTKEVT
                     PPKKTNDYRKRTDLGMATNEPENSTEGEQAVSQDNTGNVSGTAGQMKTFEPPQLNKASF
                     LVRRNNTASPQNQVKTRQQEMILAAENNATQPTDSTATTTQHGTASQAISPATVRRIPY
                     NPDLYIPENTVIPCSMDYRFVSDRAGKIRCTVAKDIWSASGNTKLIEKGTTATGIYQTG
                     ITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERFGGALMV
                     GMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEIINL
                     ITGQDIDFSNIYTLRLKNELQR"
                     /name="virB10"
     gene            complement(13342..14247)
                     /gene="virB9"
                     /locus_tag="I6K28_RS25600"
                     /old_locus_tag="I6K28_25545"
     CDS             complement(13342..14247)
                     /gene="virB9"
                     /locus_tag="I6K28_RS25600"
                     /old_locus_tag="I6K28_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001230135.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTVV
                     NTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTTQV
                     VIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKEAAA
                     RQKEWEQKQEQTQIQKALRTAQTPRNWDYTKYPGKDSYNIEPDFAYDDGRFTFLGFSPN
                     KSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNGFGKVH
                     AADNSTVSDKVERVEKTDNT"
                     /name="virB9"
     gene            complement(14247..14930)
                     /locus_tag="I6K28_RS25605"
                     /old_locus_tag="I6K28_25550"
     CDS             complement(14247..14930)
                     /locus_tag="I6K28_RS25605"
                     /old_locus_tag="I6K28_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000139290.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLITILSLIA
                     IVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYVYP
                     ALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATAPDK
                     VATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            complement(14923..15066)
                     /locus_tag="I6K28_RS25610"
                     /old_locus_tag="I6K28_25555"
     CDS             complement(14923..15066)
                     /locus_tag="I6K28_RS25610"
                     /old_locus_tag="I6K28_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825480.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPHAFSGQGSPVNTPQIIAELTT
                     HD"
                     /name="hypothetical protein"
     gene            complement(15116..16186)
                     /locus_tag="I6K28_RS25615"
                     /old_locus_tag="I6K28_25560"
     CDS             complement(15116..16186)
                     /locus_tag="I6K28_RS25615"
                     /old_locus_tag="I6K28_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_024187435.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYIM
                     WRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSSTSI
                     WQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIAEIM
                     ILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSRMSEA
                     VTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGIGAAAY
                     GAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEARMLTDQ
                     SAPKLPPSIKRLPPPR"
                     /name="type IV secretion system protein"
     gene            complement(16196..16423)
                     /locus_tag="I6K28_RS25620"
                     /old_locus_tag="I6K28_25565"
     CDS             complement(16196..16423)
                     /locus_tag="I6K28_RS25620"
                     /old_locus_tag="I6K28_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000720509.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAECQ
                     KTGSASENCKHANEAHWQIQQLNAPEISFN"
                     /name="EexN family lipoprotein"
     gene            complement(16435..17139)
                     /locus_tag="I6K28_RS25625"
                     /old_locus_tag="I6K28_25570"
     CDS             complement(16435..17139)
                     /locus_tag="I6K28_RS25625"
                     /old_locus_tag="I6K28_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000819778.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQW
                     LQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLSGG
                     SSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLGEIN
                     ELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLADERRKKWN
                     QQQLNAPEIMFN"
                     /name="type IV secretion system protein"
     gene            complement(17154..19898)
                     /locus_tag="I6K28_RS25630"
                     /old_locus_tag="I6K28_25575"
     CDS             complement(17154..19898)
                     /locus_tag="I6K28_RS25630"
                     /old_locus_tag="I6K28_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001096401.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFLP
                     IEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDAMK
                     LNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLHGLI
                     RSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFVTICY
                     RPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTENEAVF
                     SSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFRGLEVKE
                     FSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDAVSQRLDL
                     DVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVPAEISLSAA
                     YMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVRTPSGDGYYL
                     NLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDSFSPDARTKRL
                     TTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFIKQLMKILCTRN
                     GATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQENGLSIRLSQWA
                     RGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLLYRITSLLDGRRLV
                     IFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDEIAPAVIEQCGTQ
                     ILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQFRRGDTKRFAARITLD
                     LSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(19911..20207)
                     /locus_tag="I6K28_RS25635"
                     /old_locus_tag="I6K28_25580"
     CDS             complement(19911..20207)
                     /locus_tag="I6K28_RS25635"
                     /old_locus_tag="I6K28_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000608756.1"
                     /translation="MINKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVSN
                     GLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(20211..20915)
                     /locus_tag="I6K28_RS25640"
                     /old_locus_tag="I6K28_25585"
     CDS             complement(20211..20915)
                     /locus_tag="I6K28_RS25640"
                     /old_locus_tag="I6K28_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000953548.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPKR
                     ERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLFNP
                     CTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSPEKP
                     RYVVPGTRDDIATQSAILNATPVEAPTRPRVVWPGAIVRGVPAQLRQKKADTVYYPAQV
                     IRGNRFSTEKED"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(20931..21137)
                     /locus_tag="I6K28_RS25645"
                     /old_locus_tag="I6K28_25590"
     CDS             complement(20931..21137)
                     /locus_tag="I6K28_RS25645"
                     /old_locus_tag="I6K28_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204628279.1"
                     /translation="MPKPTYNVYVTQEVPTDENSGKNTYWTKVGAAFAHNGKPGLNIML
                     VPGIAVSGKLVLLEPKEDAPQGN"
                     /name="hypothetical protein"
     gene            complement(21138..21350)
                     /locus_tag="I6K28_RS25650"
                     /old_locus_tag="I6K28_25595"
     CDS             complement(21138..21350)
                     /locus_tag="I6K28_RS25650"
                     /old_locus_tag="I6K28_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001133545.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAFL
                     QYMIDKLSNEAHNEMLKELMSKEDK"
                     /name="hypothetical protein"
     gene            complement(21427..21876)
                     /locus_tag="I6K28_RS25655"
                     /old_locus_tag="I6K28_25600"
     CDS             complement(21427..21876)
                     /locus_tag="I6K28_RS25655"
                     /old_locus_tag="I6K28_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_000995159.1"
                     /translation="MNAWYLLYCNNNDLDRIAYRCKTMNVVAFCPRYIKITPRKDCNAV
                     RKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAKIL
                     ALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            22131..22496
                     /locus_tag="I6K28_RS25660"
                     /old_locus_tag="I6K28_25605"
     CDS             22131..22496
                     /locus_tag="I6K28_RS25660"
                     /old_locus_tag="I6K28_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000248966.1"
                     /translation="MVPKRLKEARMEAGLSQEKLSQLLGVAEGINTRSRFSSYEVGRTE
                     PPFKLVKKIAELLDYPENYFYTEDDDFAKAVLDFHRKRTSPELNPNYDSEVEARRMASK
                     LDNVKKIVAQLNEYLKE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            23114..23779
                     /locus_tag="I6K28_RS25665"
                     /old_locus_tag="I6K28_25610"
     CDS             23114..23779
                     /locus_tag="I6K28_RS25665"
                     /old_locus_tag="I6K28_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_001350943.1"
                     /translation="MAKQSPCYNVVSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIRH
                     NRYWVNEANKFGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKSIS
                     RASRGCDRLVWLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLL
                     KADPDEVIYSGDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTPIPA"
                     /name="RepA family replication protein"
     Typing          complement(6294..7024)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="9.03e-162"
     Typing          complement(14251..14930)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="85.463"
                     /coverage="100.0"
                     /evalue="4.2000000000000004e-119"
     Typing          complement(17158..19898)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.527"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagaaagatc acaaaaaggt tcctgttggg ggtcagtttg gaaatcggaa atttcggcgt
       61 ttccggtgta caaccctagg aaagtagatc cgctcgccgc aaccctagcg accgaacgtt
      121 agtgagcgag tgggtgagga agcggaatag tgcccttgct gaattgagca catacgcgaa
      181 ggaattaaag gtgagtggtg gaaaattttt cagttcgaat agtgaaaaat agttgcaata
      241 cctgcatatg caggtataat aaaatcatcg aaagggaaat aggttccctt tccacttagc
      301 cccatagggg cgggagatta aaatgagtgc atttattttt agaaccacat ttaaagcaac
      361 tggcgatcag caaaaagacg ctattaccca aattgaagtt atgcaggaac aagcagtaaa
      421 agcctttgtt tcctggatga ccaaccagga gggtacagaa tttttaagca ctttgcagga
      481 aatcacaaca gaacttgacg cactgattga tgcggtagag tcccattccc ctgttttcag
      541 aaagaaagcg actgaactgg aagcgctttt agcccattac aaaacgttgc tgtaagtcct
      601 caacggggca attgccccgt tatatggctc tttatttaat ccaaatctaa agcaagtgga
      661 gtttaaaaat ggctcgctta ccttatattg aatcaaccac gcaacagatt tttattttta
      721 atactttgac actttgtact attggattaa ttcatataga gactggtgaa aaatatattg
      781 tagtatttac agataacaat aagattagag attataaaaa tggtgtaata gaagaattag
      841 gggaaattac gcaagaagat attgaactga ttgagtatta tgctaaaaaa tatgaaaagt
      901 attttgatga tttaaaaata ggtgaggagc ctttgaatct taaagaatat atagaatgta
      961 tgcaatagcc atatattcta taaaggtgtg atcaggttgg tcgagcaacc tgatcacttt
     1021 atcgacactc aaaacaatag agaggtgttc aaatgtcaac aggtcagatc gtacaacttg
     1081 cggattcact tgtcaaagga tgggaaacga gaatcccagc aatgaaattt acggattggg
     1141 atcaattcag gtggtggtta aattatttac agggatatga aatgttttaa gcaatgttga
     1201 tgtcctgaca gtaattataa attttttcaa aaagagagct aaaaaacttg caatacctgc
     1261 aattgaaggt ataattaaat catcgagagg gaaatggttt ccctctcatc ttagccccac
     1321 aggggcggga gctacaaaat gaatatccaa gaagcattaa atatttttgg cttgtctggc
     1381 cagttgactg aaaaagacat caaaaacgcc tttaagaaag ctgcattaaa atatcaccca
     1441 gaccgtaatc cggtaggtgc tgaaatgatg aaggcggtta attcagcatt cgatttttta
     1501 atggcgaatc ttgataagat taatcagtac caaagtcctg aacaaagtgc aatatataac
     1561 tatggtgatg aactagaaaa tgttttaaag gctctttacg gtttgtctgg tttagtttat
     1621 gaggttattg gtaactggat ctggattagt ggaaatacta tcgagcacaa agaagcatta
     1681 aaagaaatag ggtgtaaatg ggcggcaaag aaaaaacaat ggttttatcg tccagaagaa
     1741 cataaaagtt actggaaccg tgaagagcac acgatagagg aaatccgcgc aaaatatggc
     1801 acaagcggaa aattccaggc cggaggatgg caacaagttg aaggcagggc aagagttgaa
     1861 gccagagcat aacgaaacgg gggcgtaagc cccctatatc ctcgccagca atccttttcg
     1921 ggcatcgaac ccgaaaagga ttgagtagct gacactctgc gattgctggc tacgttagcg
     1981 cagtaaagga tttgaaagca tgacaacatt ttcagaaaaa tatcagcctg tctttgaagt
     2041 tgtatgccgt attcttggga acggctggcg tgttaacaaa ctggataatt atcagcatcg
     2101 tattaaactg acttctacag aaattcgtaa atactccatc attattcgtc tggagcgtga
     2161 aagatttttt atagttggta gcgttgatag tcttgtatgg cacggtaatc cttccgtctg
     2221 tactgtatct cttacgcgca atcctgttgg tattgctaat gatatatctc ggaaaatcct
     2281 tgctaatgca ctggatgaaa ttgaattgct cagggagcat gagagcgcat tacaggaaaa
     2341 acaagataaa gtgcgaataa tcaaagggat gttatcacag ttgctttcta tatctccctg
     2401 gtatggggca ttaacgggat ttaatgcgga taatggactt gcaggaaaaa tactacaaaa
     2461 tggtgatgat tatgaaatgg ttatcagagg gcttaatgtt gatcaactta tcaaagtggc
     2521 aggttttata aaaaaactat aacaaagtgt tgatgtgaat tttatatggg gttgtttatg
     2581 aaaactgata atgttttctt taaaagtaag ggtagttact cttatgttgt tacagtagat
     2641 atggataatc agatgtatat ggtcagaaag tatgttaaga catatgtgaa atcttgtgat
     2701 aaagagcaag ttaaagggaa aaagacagtt aaaaaattgg tgctcgatac gaatcttaca
     2761 ttgccagaat tattggatct tgataatggt attctggctg atttatttac aagaggtaat
     2821 aacaatgcaa actaaaccaa ctccggcaga tgttaaagag gcgagaattt ccgcaggttt
     2881 aacgcttaag gaagctgccg aaatttttgg ctatcagtta aatacctggc aaatgaaaga
     2941 aagcgcaggt aaagctacaa ggtctttatc tgtaggtgaa tataactact tattgttgtt
     3001 ggctggaaaa caccctgaat atgttctttg taaaagggat acgatcaccg cagaaccaac
     3061 cactgtctga ccccaaaagg ggcttttcgc cccttttcta ctaaatgtta aaggccactc
     3121 gctttccggt agggagttca acatctaggc tcaactttcc tcccatagct tcaacgtaac
     3181 gctttaacgt tgccagcttc aaatcatttc cgcgctgttc cagttgagtt acagcgggct
     3241 gactgattcc cattgcatca gcaacctgtt tttgtgaaag ctgcaattct tcacgcatca
     3301 tttgcagccc tgtttccaga atcatctcat cagccatagc tttgatgatc gcctgacttt
     3361 caggtgattg agccgcaatt acttcttcta atgttctcat tttttgccct ccagttcggc
     3421 taagtgagtg gaaaattctt cgtcagcaat acgaatcatt ctgtcgtaaa atttcttatc
     3481 attacttttg tcgccagcgc aaagaatgat tgcttgtcta attggatcaa atgcaaagaa
     3541 tgccctgatt gggtgtccct catattgtat acgtagttcc ttcatattct tatgacgtga
     3601 ccctttcact gtatctgcat gtggccgtga aagagagggg ccaaactctt caagatgttt
     3661 aagttcagcc agcacttttc gttttagggc tagttcctgc tcttcaaacc attcatcaaa
     3721 taatctgcca aaataaacag tccacatgtc ccttttccta taagttatag cttatagatg
     3781 attgtataag ctacgactta taatttcaag ggaaacgggg ctatttaaca taatggacgt
     3841 tatcggcacc atcggatcac cgttcgttgt cggatctgca aaatccgctc aaaagatcat
     3901 gttttcctgg gcgttttcca ccgtttttta tgttgcgtat ttgttaataa aacgtcattt
     3961 tttgttttgg ctgaatttgg ttgtgaaagc cttatattca caagttatct cttcacttgc
     4021 cgcctgtagt tcaaatagtt gttgcaattc ttggcaccaa tacggatact ataattatat
     4081 ttttctataa aaatattttt atagggggtc gtatgacggt agctattgcg ctggttaacg
     4141 gtaaaggcgg ggtgaaaaag tcctcaatga gcagggctat tgccacagag tacacgaagg
     4201 cagactggaa ggtattaggg cttgatatgg atgttgatca ggggtcattc ctggattggg
     4261 gacagcggcg attgagatat aaagttgaac cacctgtcac tattcaggga tgcggaacgg
     4321 tatcacaggc taaaaaacat attgaatcag ggatatggga tttagtcgtt atcgatagtg
     4381 ctgcttatgc aactaaatcg ctgttgagcc tggcagaaat tgttgatttg ctcgtgttac
     4441 cgtctggttt tagtgtggac gatttgagaa cgacggttac aaccgtgaat gcattgcgta
     4501 acaagggaca ctcaacaaat aagatggctg tagtttttgt cggagtcagt gaaagcgaaa
     4561 gtgagtatcg taccgcgctt gaatatctta aaccgtgtgg tgttcaagtt atcaatggcg
     4621 caataccttt tatgacctcg ttcagccagg cacaggataa aggactggca ttgacggaat
     4681 ccccttttcc aggtccacgg cagaaagctg acgaagttgt ccagggagtt attgacttgc
     4741 tgactaaact ctcacagtga tatataatta tataattata tttttataga ggtgataaga
     4801 tggctaatgt accaccaaaa ccgcgtaaca ccaaaggcga gccgccaagt ctggaggata
     4861 cacaacacgg atttaatccg acgcgtaaaa acgctgatgg gagaaaagct cagcttaacc
     4921 tttatgtgga tatggaagtt aaacgggagt tccgctcgat tgcagcggct aatgatatga
     4981 agcacgggga gttgctggag gcataccagg aattctggaa aaagcatcat taaaatggca
     5041 taggggacga actgtcccct gatattaaca cttaccaaca taatgaatat gttcaacggt
     5101 tcctaaaatc tcaaaacgat cgggagctac ctttcgccag tgatttttgg cacgagaggc
     5161 atagcttctg tcccagcgga gaatacgatt attgtcctgg ataacacata ccatcctgtt
     5221 ttctgctgta cgcaatgatt ttgtaatttt agctaaatca ttatctggac ttagatcaat
     5281 tagtttttca tggccgtcat ttgacacaac gagtattttt atcgtcatta tcgtctccat
     5341 tatcatattc tggaaacact attagtaacg cttcgccgtt ggtgcgtttc ataaaattga
     5401 acactaatct gcctacttct tcatttgtca tatagcgttc tattggcaat gtttcgtatt
     5461 gatccgcata gagaatattc acactttctt cttctggttc tgaaatctgg ccaattgtga
     5521 tatctgtcag acctgtatca gtgaaatttc ctgattttat gtcaaagtcg ctatctttga
     5581 atttgtatgc atcctgccat acagcgaaac aagtgtggca ggtgatgggc agttcgcctt
     5641 cagccagctt ttcacaattt tgaaccagta ctcgctcttg gttgtactgt aagttatagc
     5701 cacctgtaca aagcgtttca tacccatgag cgccagttac gatatgtgtc gcaacccttt
     5761 ccaggacttt cccattcact aataactgtt cacgtgtggt aattgtttgc atgtcaatct
     5821 cctatttatg tcgccattcc cacggtggaa cgggattggg gtcagcccac aaacctcgtt
     5881 tttgtgagcg agcttcctgt tgtagatctg ccagagcgtt atctgtgttg taatgctcat
     5941 acacccatgc agcacccgat tgcaccataa agcggttagc ctcactaccg cctgttgtga
     6001 atacacgtcc gataatgcga ccgtaacggt cgttgtgtga atatgtcacc ctgacggatt
     6061 gtccggcaat caggctttta agttggtcag ttgaccagcg tccgtaagcc tgtttctttt
     6121 ccggtgcatc aatattggcc agacgaatcc ttactggcgt tttattctgt aaaatttcga
     6181 tggtatcgcc gtcaagtaca cgaataactt ttccctgtat atccgctgca ttggcatata
     6241 gtgaaggcga caagatatat aatgcgaata tgattaatat tttcatcaac tattgttcca
     6301 gttcatcaat tgttctaaaa ataaacatct ctctgttttt tgcatcaaaa ggcacaggtg
     6361 tattattcct cattacagtt ttgattgagt cgaacagtct gattaatgat tctttacttt
     6421 tggtgtcagg catgacgtac aaagcataat gccagtttcc tttatctctt gccgtaagat
     6481 gactgtttat aatgcttata tagcgagcac gagttttcat tgttcgttct gtttcaatag
     6541 ccattattgc gccagttggt tgttttatta ctccatcagg gcgatgggaa cagccggaat
     6601 aacgtgcgat gaaggctgca ctatcaccgt taatccattc agtagcgcct ttactttcca
     6661 gagatattct cactcgctga tttaaaagac gatgctcaag agtccagtat tttagtttgc
     6721 tgggttcgaa ataactaggg aacgtaatgt catcggcttt tacaaccatt gctaatcctt
     6781 gcatagtaat accccaaaca gttaattttc tttttgatga tggtatttca tgtttgataa
     6841 taaaaccttc tttaagagcc ttgtttagta atttatatac tgggtttctg cttttttttg
     6901 tgaatcctaa ttttcttttt ataatgtgaa agtctgaata cgtttcatcc ttaaggaaat
     6961 ttaaaagaca aagcatgttg ttagctgata ttctttttct ttcgtaaaaa tcatgaatta
     7021 acatagtaaa acctcataaa ccactggaag gcacatcgtt gttattgtgt tgttctattt
     7081 gttctgtctc aaaagatagc aattcaatgg tttctttgtt tgctggtata tgagacattt
     7141 tagttgcttt tggtaaatct tttgatgtga acacaaatcc tactctttca ggtagattta
     7201 aaaacatatt actgtctaca taataacttt ctgcctgtct tatcatgcgg tcagtatcaa
     7261 ctttttctgt taatgataag tcggttctca ccttcctcat ttcatcgtca actaatattg
     7321 aaccagtcat tttagctgcc cactctgctg tttctggatt ctggatttta tagattaact
     7381 tgaattttgc gttttcaata acagcaccaa ttacagaatc gccattaaga tcagaagggc
     7441 aatctcttaa atcatctatt gcctggaagg ccataaatat atgcacactt ttatctcgtg
     7501 ctgtacctaa tccttccatt gtcgggcgtg aaatatgata tttcaactca tcaagaaaaa
     7561 ttgcaacaac acgtggagtt gtgtttattc tgtctctggt ttcagctatc tgaatcaaac
     7621 gcgtaagtat catgcgttgt gcagaaataa ttttttgatt tcgggttgac ccaatgatat
     7681 agcagcatcc tccattatca aaaatctctt taagagaaaa tccatttttt gcattgacgg
     7741 aatttacaag agcaatttct tctagttcac ctaaaaatgc tggggcatct tctcctattg
     7801 attttacgtt gtcatcgttg aataaatcat acagtgtcat tccggtttta tgaattgcgg
     7861 ctacatttct ggctgcgcgg cgatcagcta tacgataaaa atctgcggcc tctccttttt
     7921 ttgatagact aaatcctgca ttaaataatt cttctaattg ttcatgtgat atgtcagcaa
     7981 gtaagtcaag ttgatggttg gttctgttta aattaattac aacaaatttc tttcctgctt
     8041 ttttacatgc ttccctaaga acatgcacag cccattcatc atcttttgga tcttcaacaa
     8101 aaacagcctc gcctaacaga attgcctgat aaagcaaaac cgatgcggta atgcccttac
     8161 cggaacctgt tgtacctatg attgctgcat gttgtttctg aaattctttt attgttatat
     8221 attgcggttg gtcgtctttg tctaagccaa tgaaaacccc ttttttcaaa tcaatgtaat
     8281 ctaggggatt gtattctatg ctgtctggca acaattcttt tactttgcga acatctgttc
     8341 ttgtatttct ctcaagtttg cttttcttta tcattctgtg tttcaggctg tctatttcac
     8401 ctgctaataa tcgtcgcgcg gtcaaatgaa aagctaatcc agctagtgta aagagaattg
     8461 tgattatcca taatggtatt ctaagtagtg tattgggatc gagaacgtca aataggtagc
     8521 tcagaccatg aatactaagt ggtgacattg tgccgaatat gaaaaagaaa atggacacgc
     8581 acgcaacaga ttttaaccag aagggagcgt tttttctttc ctctcgtggt aggttgaata
     8641 taaatggtag tgttagtcct gccagtaatg acaatatgac agggctgttc tgtagccata
     8701 acagaaatat tgaaaaggca tcagcaatac cattcagctt ttgcattaga tgtggattca
     8761 tatttgtttt ccccattcat aaagtgaatc tatggggtac gacaatgggg tacagtaatg
     8821 ggggatataa tgtaccccat agctgtaccc cattattgta ccccaataaa agccaaaaac
     8881 gttgtgatcg cctaaggcga tcatgcccgt tatccgttct ttcaggagcc agccgataag
     8941 ttatcgtctg gctcctgaaa gcccgaagaa cgggcaacgg cgtgtgccgt ttcggcctgg
     9001 ctgaaacgcc agtccttcac gtcacatggg ggtaatggcc cgtctggacg ccggaccaaa
     9061 acccccaagg tcaaaggcgg cacaccgtcg cgcgctacgc gcgaccaccc cctttaagac
     9121 gcgcgtttag cctatttttt cttcgcaagc tcgaaaaaat tggaacgctg ctttaaaggg
     9181 ggtggtcgcg cctaatcggt aatatccccc gctggggcgg gaaatattac ctggcccgac
     9241 ggtgtgcggg gcaagccccc cacaaaaacc ggcacgtggg cgtgcctttt ttctcgctgg
     9301 ggcggaaaaa tttttgcggc tccccccggc ccgcttcaat tcgcttgggg cgaatgttcg
     9361 cgggtgggcg tggtgcctga aaattaacaa tttcttcgaa attgaatttt ctgtccacgc
     9421 accactttct ttattcctgt cttaacaatt tcttcgaaat tgaagccaga aataaagaaa
     9481 gcctgaactg caccacgccc tgcgtcggac tcgcccaaat tcgcaaagcg aatttgcccc
     9541 gcccttcggg ttgctacgca atcctctgcc tcagacctgt gcccgtgtta tttcagtcag
     9601 acattcatcc tcggctttta ttgcagcatt atatctgtct tctgttattt cataaccttg
     9661 tgaagcagcg taactaatca gcttattggc atcagaactt ttcagtttta ctgtgtgctc
     9721 aattaaccct cgcattaacc tcgcttgata tatttcataa tatagcccgc atcccctgtt
     9781 tgctttatta ctaatatcat caaagactct ttttacatgg attctgtcct gattccttga
     9841 atgaatatta ttaatgaaca tatcttcttt taacatagga ttctccagat ctgaaagaat
     9901 atgttaattt tccctcttcg attgcaatgc taatgcttcc gattctgtaa tgagaataat
     9961 atcaggatat cgtttcttta aatcttcaag cgtttcgccg atagaagtaa tgaaattccc
    10021 tttcattcct gatggaatgg agcacactat cttgtgtgca tagctataaa caagatcgga
    10081 gtttgtcaga taaaatacca tgttttcatc tgtattcata tttaccctcg aactctacca
    10141 aacttactca ttatttttga tatagctgcg ggatcagcgg atgaacactg atttaaaaat
    10201 gctttacgtg catcaaatgt tttactccat cgtattgaac ctttcttttt cttaagaatg
    10261 ttgaagaatg ctttttctgc tgatctgcat tcgccacctc cgctgtttcc tgtagctttt
    10321 ccgtacatac ataaaacaac ctcgcatggg tctgcggcat aagagtaaac aggtgtggcg
    10381 gttgttaaaa caaacattgt tattgctgca atttttttca tgtcagaacc ctttgtttaa
    10441 tattttgtgt taacgcgaaa agtagatggt tgtttcattg cacgtaagac aatttgtgat
    10501 ggggtgatat ataattaccc cgtttgcacc atgatgatac aaccagtttt ttgcactgaa
    10561 ataatcagcg cctgactttg ttgctactgt gatttgtgtt gagtgagcaa tggcataaaa
    10621 gacagatggc atccaatctt ctttattgaa atcttgtgag ctaatggata acgtccattt
    10681 gtcattaata acaggcgatt ttattattaa gctattgggg ttccattcag gaagtttctc
    10741 gttaagcgat accggctttt tattccattc cacgggtact ggtggaggcg gtgaggaaca
    10801 tccggtaatt agtgttacaa gcacagtaac cagtatatgc tttatcatac tttcatttcc
    10861 tcgtaatatt gattgcggtg taacggtcgg aaataaatat cattcatacg gcgtacgttt
    10921 ccatctaaat caatgctcac aacaacatcc agagagtcga gaactttacg tagcaagaat
    10981 gaatatggta attgcgcaca ctgtgggttt tcagtacagc gggtaataaa accgtcgatt
    11041 gcttcatggg ctgaaccagc atgaatggag gtcatacttc cttcatggcc tgaccccgtt
    11101 attttcagaa aatcccatgc ctctccaccc ctcacttctg ttaataaaat tctgtctgga
    11161 ttcatccggt aattccagcg tataagcgcc gctggtgtta caacagaacc tttttcatcg
    11221 gatgactcag aaggataaaa cagatgaaca tagttcttat gtttgtagaa tattatctct
    11281 gggttgtctt ctatcgtcgt tatgcgtaaa tggacgggta tatattgtat caacattttc
    11341 atgtatgttg ttttacccgt actggtttca cctgcgacgg ctaatgtttt tccatactcc
    11401 acacattttt ccataaacaa aggaatgttt tttgatttgt ataattcaat caattcttcg
    11461 ttatgtgtgg atgctttttc aatgcctgtg acgcggttat aaaaaccgct atcaatatat
    11521 gcctgatgcg gaatttgaat ttttgacggt ttacggatcg tgattgagat cgtgtcacgt
    11581 tcgcacgccg gaggtacgac aacctgacat cgttctcctg attccagggt tgcagaaagt
    11641 cctggtttga tatcctcgat attatcgtta tggtgttttg caagacaccg agcaaagtta
    11701 tagcaatcgt caaatttcag atcaacggta tgttgccccc agacaccatc tatctttgtc
    11761 cagagttcac ctggacgatt aacagctatt tctgtcagtc ctggtaattc cagaaagtca
    11821 ccaaaaaatc gctgtttata gcggtccagg gaaatattct tagcggtcat cgtttgctcc
    11881 tttcagtgtg attctggtgg tacaccgctg caaaacggcc tgtgcggttt tgtttttaat
    11941 cagacgctgg taagcatcct gtgcggcggc ttcgttcggg ttcccggcga gaccccccat
    12001 gtgtccggcg gcgagcatgt gcaaccaagt ctctttatct ttgcagttca tttttcagcc
    12061 tcagcgtgta gatatttgaa aagtcaatat cctgccctgt aatcaggttg ataatttcgc
    12121 cctggttttt gtagagcgta ggggggatat tgatactgtt ttccagcgtg gttcgagcca
    12181 tatccgccat agcctggcgg ctgttctctg tatagtctgt attgcggtct tttttacccg
    12241 cattgtttga tgcccatgcg ccaatatctg ggatcatgcc gaccattaat gcgccgccaa
    12301 acctctccca gaagtgggta tcaatccatc catcaacacc tgattctcca agctcgccag
    12361 cggcgttggt atcaatcagc ggaatatcca gatatggtcg ctgtcgggtg cgtaattttg
    12421 tcatcatgat aaaagcgcgc ccctgccctt gcgtaattcc tgtctggtat attcctgttg
    12481 ctgtggttcc cttctcgata agttttgtat tgccgctggc actccagata tctttagcta
    12541 ccgtacatcg tatttttcca gcgcgatcgg aaacaaagcg gtaatccata gagcatggaa
    12601 tgactgtgtt ttccggtatg tacaaatcag ggttgtaggg gattcttcgc actgttgcgg
    12661 gggaaattgc ctgtgatgca gtgccatgtt gtgttgtggt tgctgtactg tctgtaggct
    12721 gtgtggcgtt gttttccgca gccagtatca tttcctgttg tcttgttttt acctgatttt
    12781 gggggctggc cgtattatta cgacgaacaa ggaagctggc cttattcagt tgtggtggct
    12841 caaatgtttt catctgtccg gcagtaccgg atacattgcc cgtgttatcc tggctaacag
    12901 cttgctcacc ctcagtcgaa ttttccggct cattggtggc catgcctaaa tcggttcgtt
    12961 tccggtagtc attcgttttt ttaggtggtg taacctcctt tgttttctct ccattttctt
    13021 gcttcaggaa gttttgatat acccagttac cgccccatgt caggccaatc agggctactg
    13081 cgcatattgc cagaaaggcc gctgctttcc ccttctgacg ttttttcagt ttgtttactg
    13141 ctggttgtaa tgagttctcc ttttcatcgt cctgtgatgc ttgtaattca gcctcacgtt
    13201 ttgctctggc ttctcgttcc agcgtggcga tatcatcctg atcagatgtc atcactggct
    13261 tttcttcttc cgtagagggg gatttatttt cgtcagtcat atatgagtcc ttatataaaa
    13321 atataattat atttttatag attaggtgtt atctgttttt tcaacgcgct ccaccttgtc
    13381 agatacggtt gaattatcag ccgcgtgtac ttttccaaag ccgttatttt tcagccccac
    13441 tacggcatca ccggaacgta atacaagccg tggtgtgatt tcctggataa cgagaacggt
    13501 aaaattgccc ttacgctgta tactgctgtt aaccacgtat tcctggccgt tctgttcttt
    13561 ggtaactgat ggaactgatt tgttgggtga gaatccgaga aacgtaaagc gaccgtcgtc
    13621 gtaggcaaaa tcaggttcaa tgttgtaact gtcttttcct ggatatttcg tgtagtccca
    13681 gttacgcggg gtttgtgcgg ttctcagtgc tttctgaatc tgggtttgtt cctgcttttg
    13741 ttcccattct ttctgacgcg ctgctgcttc cttattagct ctgttacgtt cgtctcctgg
    13801 atagcggtaa ctcaccaggt atgcgggctg ttgtggcgat ttttcatcaa tgacattcag
    13861 ctcaacgttg tacaggcgct tatttgtcac aatcagcata ttggtatgcc agtcacggct
    13921 gttcggcggg ataacaacct gtgttgtatt accgtcttca ccaggtgcgc cctgtgtgag
    13981 cgcaaccgga cgaatgttta ccctgtttgc atctgctctg gcttcccatg cttcatcaaa
    14041 gccaggactg gcactgataa cagcttcatc gttatcaaaa accagagtcg tcataaatcc
    14101 tggttttgtg tttacaacgg taacattctg cgggttataa atgacctgct ggatacgtgc
    14161 atcataacgg ctcgcttgtg gtgtagctgc ggcccatgcc tggctgttca gcattattat
    14221 cagggtaatt aacgtcagtt ttctcattac tcgcctcccc tgatttcgcg gtcagtctgc
    14281 cagcttgtta cggtgaatcc gaacgggttt atttcccgct caacatctga catttctttt
    14341 tcagggtctg aatggaaggt gaaacgtgca tcccagtatt cattgcgaat actgttatca
    14401 gccatgcgac gaattatttt tttataacga acggtggcta ctttgtctgg tgctgttgca
    14461 ttcgttatct gctcagaaat aatttcaatt ttcacggtgt gagccgcgtt attataaact
    14521 ttgtctggtg cgttttttcc ggcgtacagt gccaggtagt cgtcgtttac ctgtggtgag
    14581 ttgtagatct gtactgtctc gtaatcgtcc tgtaatgcag gataaacgta ccgttcacgt
    14641 aatttaacgt aattggcggc catagctttt tggtaggctt cttccgctgt cagggctgtt
    14701 tttgatgcac gggtaacgtg ttccactcgt ccggtatgat tatctacggt atagagttca
    14761 actgttgttt gtttaagtgg caacataatc actatcgcga tcagtgacag gatagtgatt
    14821 aataaccctg ctccaccgac cagaaagcct gcttttgtgg cgcgtttatc cctgtcaatc
    14881 atttgctgct caaacgagcg ggaatcagca attaatttct gttcagtcat gtgtggttag
    14941 ctccgcgata atttggggtg tgtttacagg gctaccctga ccgctgaatg cgtgcggcag
    15001 agatttagat ttttgtgcgc agccagaaag cacgcacagc aaagccagcg cgattaagcg
    15061 tttcatttta tgtacctttt tgtatttcta taaaaatata attatatttt tatagttatc
    15121 ttggaggagg taaacgttta attgatggcg gtagttttgg tgctgattga tctgtcagca
    15181 tccgcgcttc attacgcgct atcatttgtt ctatagcgtg tttacgtgca tcagcagcgt
    15241 taaatactat gcgcgattgt ggatctccta accctttatt acttgggctt gctacttctt
    15301 taggtaatac ttttcctgcc tcctttaaca tctttccagc gccgtatgca gcagcgccaa
    15361 tgccgagagc cgccgcacct tgcatagtgg ctgtagcact tgctcctgca agtgccccag
    15421 ctagttttgc ggacatgtac accagtattg cagcgcatac tccggcaaga caaacttgag
    15481 cgccaagttc tacaatgttt gctttgctgg cttgggtcac tgcttctgac attctggatg
    15541 taagaaaacc aattacaatt cgcagagcta aggctgaaaa cataattgtt agtattgcag
    15601 caaatatatt ttgaagccag ttattaaaca ttggccgtaa aaagccatac atcaggcaaa
    15661 atataaatat tggtgcggta atacctaaca ataagatcat gatctccgca atcatagata
    15721 cgaatgctgt gataatcatt aagacaaaga caccacccca tacaaataat tcagcaattg
    15781 tgccttcctc tttgataaat tcggagttgt ctagatcatg taacgtttta cctaaaactc
    15841 ttgcgctgtt ccatagagta tcaagtaatt gccagatgct tgtgctactg gaaaaacctt
    15901 cttttattcc atttacagca tcaatgacac catcaagata tccgttaatg tttgctacaa
    15961 aggataaaat gatagccatc ctcattatat cccacataac atcttccatt ggtgtagaca
    16021 gctttcccgc taaggtttga tatcctcgcc acataatgta gatagtggct gacgtagccg
    16081 ccagccccat catcattgac gaataagaca tcatcaaacc attagtcgcg tcagttatcc
    16141 caccgatgat atttttttcg acagaagaaa atataccggc catgataaac gccctttagt
    16201 tgaaacttat ttcaggtgca tttaattgtt gaatttgcca atgagcttca ttggcatgtt
    16261 tgcagttttc agatgcgcta cctgttttct ggcattcagc ataaacaact ttgagttcat
    16321 caggatgttc acgataccat tttgttgttt tagcttcttc cttacatcct gaagttaacg
    16381 ccatcaggcc tgaaataatt gtaaataaaa tgattttctt cattaagtca cctgctaatt
    16441 aaacattatt tctggtgcgt ttaattgctg ctgattccat ttcttacggc gttcatcagc
    16501 aagcatcttt tcacgcttct ccgccgcttt tacgttcatt tcccattggg tcgtgagcgt
    16561 atttagcatc acagatttta actgaatact gtttgctaag tcttgggcct ctttcatatc
    16621 tttggcattt gctacacggt ctgataattc gttgatctca ccaagcatct caccaatctg
    16681 ggtttgtact tcattggttt gttcgatttg tattgctttg ttgatgacct cttgtctgca
    16741 tgtattggca taactttttg attgttcagc attgcagtta tcgaaaatct tgtatttgtt
    16801 gtacagttca tcaaattggc cagaagaacc gccggataag agcaaatcgt tcagggcttg
    16861 ccctggctta cgtagtttat ccaggtcagc tttaaggctt ttggcctgat taacgaacgc
    16921 ctgaatgtca cggatgcctg taactgtttc cagttgtttt tgatacgcct gtagctggtc
    16981 tttatagtgt tgtaccgtct gtagccattg attggcttct tttgtccact ggcgaatatt
    17041 ctctagtctg gctgttgcat caaacacggg tataccagca gcgtttacta tcgggctggc
    17101 aataagaacc gccagaataa gggggcgaaa tcttttcatc gatatctcct tggttatagt
    17161 gctttagcta ggtaggaatc cagccagtcg ctgggcttca ttccctcctg atagagtgat
    17221 tcgaatattt ccaggttagg tgcgtcgccg ctcattactt ttgtgtatct gccgatacca
    17281 gacagatcca gcgtgatacg ggctgcaaag cgttttgtat ctccacggcg gaactgattt
    17341 ttgacaacga catactgtcg cgcctgtgga tcaagtcctt taatgacatc aaacacttct
    17401 ggttcaaatt tcattccatc cacgtaatga gaacgatctg cgcttgggtt ggcggccagt
    17461 atttgagtac cacattgctc aataactgcc ggagctatct catccttgat tatttccgct
    17521 ggagattgcg tgccaacaac cagcataccg ttcagttttc ggatagtttt cagcttgtta
    17581 tacgcaaaat ccttaaatac agggtcgcgt aaccacttcc agaattcatc catgaaaata
    17641 acaagtcgtc tgccatccag caggctggtt atccggtaca gcagataaaa cgagataggg
    17701 gcgcagacat tcgggtcatc cagaaattca gtcccgtcaa ttccgaaatt atcgcagttg
    17761 ctgatatcga aagtgtcgtg ttcgttatca aacacccaac caaattcacc accgcgcgcc
    17821 cactgtgaaa gacggatact caggccattt tcttgcgctt cacgcgttgc cggttccggt
    17881 aggttctcca gcattcgggt tatgccgtag acgcgatatt gaggctcgtc actcatgacg
    17941 gcatttacag catcattgag gcgacgttca tcacgtggcg ttatggttgc accgttgcgg
    18001 gtacaaagga ttttcatcag ttgcttaatg aaattgatgt ttcgcttagt ggctggcaga
    18061 gcaaacggat tccagcctgt tggctcaccg ctgaataccc tgtagtactt tcctccaagt
    18121 gcgcgaatgt tgatttcagc accacggtct ttatcgaggt aaactgtggt tagtcgttta
    18181 gtcctggcat ccggtgaaaa gctgtcatca cggttatatt tttgctgatt gatttgtaaa
    18241 aacgtcatca gcattgtttt acctgagccg tttgtaccca gaatgcaggt acttgcagga
    18301 tttttttcgt tgaactcgtc tttatctgcc agagtgttat gcagattcag atagtagcca
    18361 tcacctgacg gggttcgcac cagagccatc gcgtcacccc acggggcttt gtcgcgtttt
    18421 cctggataga aattatgaag tgatgccagt tcaacaaagt tcaggctact caactctccc
    18481 gctcgtggcc gcagattgta attgcctggt aattgagcca tataggccgc agacaatgag
    18541 atttcagcag gaacaggggt tatccctata ttgttgagtg cattgctgat ctcgtttgtg
    18601 tctgcaatca gactttccag tgacggcgaa aacaccataa ttgataaatg atgtttaccg
    18661 taggcgattt taccggaggt gagcaggtca agcgccacat cgagatccaa acgttgagat
    18721 acagcatcat catcagcact catcatcagc cgtcgggtgc gcttaatcgc ctttttcgct
    18781 tcctcacggg acatgcaggt ataggattgt gtaatcacat aatcgcacgg cgcgtacagt
    18841 aaggagtctg tcatgcccgt tgcggtttct tccgaaaact ctttaacctc cagaccacgg
    18901 aaatattgag tgccattaac atgatttatt tgccctgatt cggcggagaa gtacagcgca
    18961 acatttccca ttacgttgta agcgggggta cgtgtaactc tgacttttcg ccattgatgc
    19021 gtgagaaggt attcataaaa tgccagttgg gacgagaata cagcctcgtt ttcggtgaaa
    19081 gtaccgagcg cgtgagcacc ataacgggat aaggctgtat ccagcgtact tttgatttcc
    19141 agcacttcaa gtaatgcgct gtctatctct tttagcttct gaccgtcttt catacgttta
    19201 cggtttgcct tatccagact ggcaaacgga cgatagcaga tggttacaaa caggcggtta
    19261 cgtcggaatg gccttttatt cagtgatgcg taatagcgtt cgctcacggt atccgcgtaa
    19321 gggtttcctg aaaccttatg cagtgagtcc gtaaactgtt cacggctgtt atgtacataa
    19381 aatgtaaccg gaaagccttc aaatgagcgt attagcccgt gcaactggtt tgtaccgaac
    19441 tgtagtgtct cgtcagattc acaatcaaac ggcgttcctg tcagttccca ggtacagaaa
    19501 aaatcgctat tcagtgaacg gacaacatta gggtgaatat gtgacgagta aggaatgtat
    19561 ttttttatgg tggcacattg gtttagcttc atcgcatcga taaactcctg gatatcaaca
    19621 gcattatgtt caacaggcat aaatgcggtt gcgccaaata aacggtttgc taccggatta
    19681 ccccttaccc ttagccagac agctatcagg ttaaatctca gaggttcatt tctggttagt
    19741 gatttcataa ccagaaactc aatagggagg aagagaagta gggctatgga agtccagata
    19801 gcaacaataa ttgtcacgcc cacgacagtc agcaggggca ccattggcac cccccacagc
    19861 gccgccggac gtttcatcgc cttaagcagc tttgccatat gttcggcctc ttagctcatt
    19921 agcagagcgc caatttcact gcctccgcca attaaaatgc cgccaatgat gatgtaaccg
    19981 cactgcgaca ggctttcgcc tttccacaga gtcttatagc caatccagat cacagcgacg
    20041 gtgatggtga cggctgccag accatgcaga ccattcgaaa ccttttccat gatggtattt
    20101 gctcggctga aaccgtcagc cgcgaaaacg ggactgacaa cacttactaa cggagccaga
    20161 atataagcag atgcgcggct gcaaatggcc tttaatttgt taatcatcgg ttaatcctct
    20221 ttttcagttg aaaatctgtt tccgcgtata acttgtgcgg ggtaataaac ggtatctgcc
    20281 tttttctggc gcagttgagc aggaaccccg cgcacgattg cgccaggcca gacaacgcga
    20341 gggcgtgtcg gagcttcaac gggtgttgca ttcagaatgg ccgattgagt tgcgatatca
    20401 tccctggttc caggaacgac atagcggggc ttttcaggtg agtaaccaat tcgctgtatg
    20461 taactggttc tggataacgc agcttctggt tgttgtcctg tagtaaaatt tccagagtaa
    20521 taacaactca gcgcccgttt cagtgtgccg cctcgctggt agcaatcagt gatgatcttt
    20581 tcaaaaacag aaagatttgt gcacgggttg aacaggtcag cagccgttac ggcgtaacta
    20641 ttaaaatttg tacttgtgat ttgcatcaga ccaacagaat agcgacgacc ttttgcctct
    20701 atctgtttga cgatgcttaa tgcatcctcc ttagtttttg gcatatgtga gataaagccc
    20761 ttgtcgcctg gctggcgttc acgtttgggg atgatctcag caatagcgta gggattaaaa
    20821 ccagactcga cccgcgcaac atccagtgat gtggaggggt gaactgtggc ggcacactgc
    20881 atagccaccg ccataaatga aacggtggaa agcatagtac cctcgctaaa ttagtttcct
    20941 tgtggggcgt cttctttagg ttcgagtagt accagcttgc cggaaacagc aataccagga
    21001 acgagcataa tattcagtcc tggttttccg ttatgcgcga atgccgcgcc aaccttggtc
    21061 cagtaggtat ttttaccgct gttttcgtca gtgggaactt cctgtgtgac gtatacgtta
    21121 taggtgggtt tgggcattta tttatcctct tttgacatta gctctttcaa catttcgttg
    21181 tgagcttcat tacttaattt gtcgatcatg tactgcaaaa aggcacttgg acgaatattt
    21241 acctccgcaa cataacttat atctctggcg tgttttgcca gtgtttcata gcgttttttg
    21301 ccgatgtaaa taacggctgt agatttagcc tggcctatag ggttcggcat gagtttatag
    21361 ctcctgtatg aaatatccgt gtgcaaaagc acagcggtac gactggagaa ggaatgaagg
    21421 tctggtttac tgattctgta aaagcctgga gaaggccgtt tgacgttcta acggtgagat
    21481 gattgaagat atgcgttgaa tttctgccag taggtcacta ccgatgttac ggcactctat
    21541 ggaatcatca tcaggattta gcgccagtat cttggcgcat ttaatagcga taacgacttt
    21601 ttccggtaca gtacatattg tggaaccgaa acttacgaag ccaattgcac ctggaataga
    21661 ggttattgct gatgtgtgcg tgacattcac atcaaaatgg aggaagaggt agttaggaaa
    21721 taaaactttt tcttccttgc gtactgcgtt gcaatctttc cgaggtgtga tctttatgta
    21781 tctcggacag aaagccacaa cgttcatagt tttacaacga tatgcaatgc ggtctaaatc
    21841 gttgttatta caatatagaa gataccatgc attcataagc gtcaccttac ggacacaagc
    21901 ccccattttc cgccgtccta agttgccggg cttaggacgg cgtactcatt aagtgttaac
    21961 tgtaaaaata cacagttgtg attttataca gcaattcttg cgtgtcaata tcaagaatct
    22021 aaagaaataa tagcattact cgattcaaaa tcaatcccga ttcattatgt tgactttgag
    22081 gcctgtaacg ccctgtaaga tgggattccc aaaaaaaggg aggttgtatt atggtgccta
    22141 aacgactgaa agaagcgagg atggaggctg gattatctca agaaaaatta tctcaacttc
    22201 ttggtgtagc ggaaggtatt aacactcgat ctcgattttc aagctacgag gtaggcagaa
    22261 ctgaaccacc ttttaaactt gtaaaaaaaa tcgcagaact tcttgattat cctgaaaatt
    22321 atttctatac agaagacgat gattttgcaa aggctgttct tgattttcac cgaaaacgta
    22381 cgagtccaga gctaaaccct aattacgatt cggaagtaga agcaagaaga atggcatcta
    22441 agctggataa cgtaaaaaaa atagttgctc aattaaatga atatctaaag gaatgagtgc
    22501 ttaggcatca gtgcgtatgt gctccatttg gtcaagtcgc ttttccgctt cctcacccac
    22561 tcgctcacta acgttcggtc gctagggttg tggcgagcgg taaataccct attaaattcc
    22621 gttatttttc cgtttgttat cgccgatttc ttttcgatat taatgctaat gtatgcgtac
    22681 attagcatta atatcgtgag ttatatgctg tacagtattt aatctcaatt ttgttaagaa
    22741 ttatataacc agatgaagtt taaagaaaaa taggaaggta atcggcggga tcttttgtgg
    22801 atgaattgat cttcgaatgg gttcatgcta ttttcgtgcg gtttttacgg atttttgggt
    22861 gggacgttcc cgtaacagcg tctaggggaa ggggggggaa gcattttagc ccccctgaat
    22921 gcaacaaccc cctgatatct ctcttcgatt ttgccggtcc aagaagataa tcagggggtc
    22981 caaagcaggc tgggtgcctg caaaggatta tagcaaatgc atttctcaat acaagacctc
    23041 tgtccggcaa taagtgcggg cggtggtggc tgaaaagaac aactccttaa tcccccgcat
    23101 atcgccgtca ggggtggcaa aacaaagtcc atgctataac gttgtgtcgc gtaagcgacg
    23161 ccgtttcctc aatcgctgga ttaaatcgct gacgacggtc gcagggcgta tcaaaatcaa
    23221 aaacgaactc cgccgccgta tccggcataa ccgctattgg gttaatgagg ctaacaagtt
    23281 tggcatagag gctctttgcg aattgatgtt agctattctg gacgatctcg atttacgctc
    23341 gtgggaaacc aggcataacc ttgaaacgct tgccgaacgt gccggactgg ttacacgcag
    23401 cgatgcggga cataaatcca tttctcgcgc cagtcgtggt tgtgatcgcc tggtttggtt
    23461 gaatgccatc attacagaga aggctccgtt taacccgtat gatgcacggt gtgcctgtaa
    23521 acacattgaa gtaaccgagg atttctttgc catccttggc attccgttaa aactggttta
    23581 tcgggaacgt gccaggctgt taaaggcaga tcctgatgaa gtgatttact ccggtgatgc
    23641 cagacttata gctatcagag ttcagaactg gacgcggaaa gcggcagccg gattagccag
    23701 gatgaaagca aaacgcgacg cagctcgaca acgcaaaaaa gaatattacg cacattcacc
    23761 tacccccatt ccggcctgat gctggctact taccgcctta tggcggtata tcgtcacgtc
    23821 cataataaat ctctgtacaa aaaatagtct tagcgtgaga tatttccgtt ttccaagcgg
    23881 ctccctgctc aggaaagagc agccataggt tatggccaga gggtaaacga tgtacagctt
    23941 gtggataatc ataagtgaag tgcaaacgat atgacgcaat tactcttttg cttctctttt
    24001 agttacttac gtactaaaag agaagcaaaa agaaaaacat tactgttatc cccttttaca
    24061 agtggataac tgaaaaacat cggcgcaagc gcctcaaagg tagcacccct cgcaagcatc
    24121 ggggcattag agacaccata tccggtcatt aaaaacgaac aatcattcac ctatcatcgc
    24181 tgtaacatcg acggtctacg accacctacc acctaagtag ctgtaagttc cgtgcaattt
    24241 tggcttagca gggggctgac gcggttcctg gcgacatgaa cgtccttgat taggggtatc
    24301 ggcgcgcctt agcatttgtg attctatgga actatatttc gcgccttaca tgccgctaac
    24361 gcggcatctt cccaccatcg agcataactt aactaactgc taacaatatg atc
//
