LOCUS       NZ_CP070091             8868 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1306 plasmid unnamed3.
ACCESSION   NZ_CP070091
VERSION     NZ_CP070091.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357448
            Assembly: GCF_016904295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1210.1x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,350
            Genes (coding)                    :: 5,228
            CDSs (with protein)               :: 5,228
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 61 of 122
            Pseudo Genes (incomplete)         :: 62 of 122
            Pseudo Genes (internal stop)      :: 21 of 122
            Pseudo Genes (multiple problems)  :: 19 of 122
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070091.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1306"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1306"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            492..929
                     /locus_tag="I6K36_RS27165"
                     /old_locus_tag="I6K36_27175"
     CDS             492..929
                     /locus_tag="I6K36_RS27165"
                     /old_locus_tag="I6K36_27175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101857179.1"
                     /translation="MQQAIRMRIEADKAALDAGFTTNNFVGELGEKLAHAVYGGHILPN
                     SAKSADIQTDDGTLLQVKTRKPNKGKQFGSIRSWDFDYLIAITLGDKYEVIRALKIPVS
                     TCKELAEHRAHTNSFIIKPTRKLLSHADIEDVTDKFQSVFL"
                     /name="hypothetical protein"
     gene            complement(1000..1236)
                     /locus_tag="I6K36_RS27170"
                     /old_locus_tag="I6K36_27180"
     CDS             complement(1000..1236)
                     /locus_tag="I6K36_RS27170"
                     /old_locus_tag="I6K36_27180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101857178.1"
                     /translation="MESIQRRFQRWKVIMIPGGKSYESWDEMVTGEDFPEEARQTYKTH
                     RTKAIDRTLKQPDYVHTMSESGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            1471..1731
                     /locus_tag="I6K36_RS27175"
                     /old_locus_tag="I6K36_27185"
     CDS             1471..1731
                     /locus_tag="I6K36_RS27175"
                     /old_locus_tag="I6K36_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018632216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101857177.1"
                     /translation="MSTDKQNNDNAAIAGAIGGAAGAAGSVGSVAAAGSVAGLGATGIT
                     SGLAAIGSIVGGGMGAGLIVTAAAPVAVGAAAYGLYRWIKK"
                     /name="hypothetical protein"
     gene            complement(1891..2163)
                     /locus_tag="I6K36_RS27180"
                     /old_locus_tag="I6K36_27190"
     CDS             complement(1891..2163)
                     /locus_tag="I6K36_RS27180"
                     /old_locus_tag="I6K36_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032725101.1"
                     /translation="MATDAQIKQINALIKKYPDSCSICQQDYDEDYVTYTVFGYDKNRK
                     MQVTTGCCARTLTELVLLGVCGCFDPDERDEIMKNHPMAKKFFTN"
                     /name="hypothetical protein"
     gene            complement(2600..2815)
                     /locus_tag="I6K36_RS27185"
                     /old_locus_tag="I6K36_27195"
     CDS             complement(2600..2815)
                     /locus_tag="I6K36_RS27185"
                     /old_locus_tag="I6K36_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2822..3319)
                     /locus_tag="I6K36_RS27190"
                     /old_locus_tag="I6K36_27200"
     CDS             complement(2822..3319)
                     /locus_tag="I6K36_RS27190"
                     /old_locus_tag="I6K36_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3360..4058)
                     /locus_tag="I6K36_RS27660"
                     /old_locus_tag="I6K36_27205"
                     /pseudo=""
     CDS             complement(<3360..4058)
                     /locus_tag="I6K36_RS27660"
                     /old_locus_tag="I6K36_27205"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3997..4338)
                     /locus_tag="I6K36_RS27200"
                     /old_locus_tag="I6K36_27210"
     CDS             complement(3997..4338)
                     /locus_tag="I6K36_RS27200"
                     /old_locus_tag="I6K36_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4969..5082
                     /locus_tag="I6K36_RS27665"
     CDS             4969..5082
                     /locus_tag="I6K36_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_087796368.1"
                     /translation="MHEPPVQSDCCALSGNYRLDSNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            5447..5686
                     /locus_tag="I6K36_RS27205"
                     /old_locus_tag="I6K36_27215"
     CDS             5447..5686
                     /locus_tag="I6K36_RS27205"
                     /old_locus_tag="I6K36_27215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153677472.1"
                     /translation="MKPGGAGLFKTQLPSTKNAIQLLADESKQATLRFPNLSFALFSHQ
                     FMWLADDFATQKKWKDKLLAFLATPIFSRVNQNI"
                     /name="hypothetical protein"
     gene            complement(5633..5815)
                     /locus_tag="I6K36_RS27485"
     CDS             complement(5633..5815)
                     /locus_tag="I6K36_RS27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087796369.1"
                     /translation="MKPGNITMRSGQYEIVGPRGGHTGVEVTSVKGKPLPPTPKSGQSY
                     VLVDPTKNGSGKKSK"
                     /name="hypothetical protein"
     gene            6042..6335
                     /locus_tag="I6K36_RS27210"
                     /old_locus_tag="I6K36_27220"
     CDS             6042..6335
                     /locus_tag="I6K36_RS27210"
                     /old_locus_tag="I6K36_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_087796370.1"
                     /translation="MLSEKIRELRTSRGLSLDQLASNVGSTKSYIWELEKKPGIKPSAE
                     LVSKLAQALDVTMDSLMDEKKENDQDIVFFREYKSLSEETKVQLMNILRAIK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            6344..7108
                     /locus_tag="I6K36_RS27215"
                     /old_locus_tag="I6K36_27225"
     CDS             6344..7108
                     /locus_tag="I6K36_RS27215"
                     /old_locus_tag="I6K36_27225"
                     /inference="COORDINATES: protein motif:HMM:NF017885.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="WP_087796371.1"
                     /translation="MTTPRLQAALVSEQWLRGKHFADALPVDCIKIAESLGIKVQGAPL
                     KDEFQGALFITPDVKAIIFNENIKEDGRKNFTIAHEIGHYCLHSDREEVQCRLEDMGAF
                     VLTPHGKEIEKEANEFAACLLMPEKDVQQQLLDKNLDLKLVLGLTLRYGTTVTATACQM
                     VHVSDKPLAVVMLNEDNTTKWCLRNSHFRDFFIKKGTLINIQEHLPEELSPTYQDTWFS
                     SSVKSVWNLKASSLDMEEYKQRIYMISGSKLT"
                     /name="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(7960..8361)
                     /locus_tag="I6K36_RS27220"
                     /old_locus_tag="I6K36_27230"
     CDS             complement(7960..8361)
                     /locus_tag="I6K36_RS27220"
                     /old_locus_tag="I6K36_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087796372.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHYGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8361..8552)
                     /locus_tag="I6K36_RS27225"
                     /old_locus_tag="I6K36_27235"
     CDS             complement(8361..8552)
                     /locus_tag="I6K36_RS27225"
                     /old_locus_tag="I6K36_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(4427..4606)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.153"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          2..467
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.678"
                     /coverage="99.0"
                     /evalue="1.07e-119"
     Typing          4587..5078
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.535"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          8601..8868
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.029"
                     /coverage="92.0"
                     /evalue="5.230000000000001e-73"
     Typing          complement(2485..4007)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4004..4317)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.3500000000000001e-58"
ORIGIN
        1 cctggtagct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
       61 ctgttatggc cgcgttttct cattccacgc ctgacactca gttccgggta ggcagttcgc
      121 tccaagctgg actgtatgca cgaccccccc gttcagtccg actaccgcgc catgtaaggt
      181 aactatcgtc ttgagtccaa cccttacaga cacgtcaaat cgccattggc ggtagccact
      241 ggtaaagggg tgtacaagag attactggtg cagagttctt gaagttgaag cctaactacg
      301 gctaaactga aaggacgtat ttggtggcag cggtcttgta caccctgtta ccacggttca
      361 gaagttcccc aacttactga accttcgaaa aaccacctcc ccaggtggtt ttttcgtttt
      421 caggacaaga gattacgcgc agaataaaag gatctcaaga agatctttcc ccatgaaggg
      481 ctacaaaaag tatgcaacaa gccatccgta tgagaatcga agccgataaa gcagcattag
      541 atgctggatt tactacgaat aattttgtcg gtgaactggg agaaaaatta gctcatgccg
      601 tatatggggg gcacatactt cctaattcag ctaaaagcgc agacatacaa acagacgatg
      661 gcactttact ccaagtaaaa acgaggaagc cgaacaaagg aaaacagttt ggtagcatcc
      721 gctcttggga ttttgattac ctgatagcga tcacgctggg cgataaatac gaagtaatca
      781 gggctttaaa aatcccagta tctacctgta aagaactggc cgaacatcgg gcacatacga
      841 actctttcat aataaagccg accagaaaat tactttcaca tgcggatatt gaagatgtga
      901 cggataaatt tcagagtgtt tttttatagc aggtcttata acaacaccat aaagccttaa
      961 tgaaccaaca tccctgttgt gctctggcta attgtacact cagcgccacc gctccagcct
     1021 atatctgacg gtgataccac tttcagacat ggtatggacg tagtcaggct gttttaacgt
     1081 ccggtcaatt gccttggtgc ggtgcgtttt gtacgtctgc ctagcttctt cgggaaagtc
     1141 ttcgccagtg accatttcgt cccaactttc ataggatttt ccgccaggaa tcataatgac
     1201 cttccagcgc tggaagcgac gttgaatact ttccattttt caccctgttc agctaaatcc
     1261 ccaaccattt tgctacagcc gccagcccat gcacacgaga aaaaatgtgt aagatcactg
     1321 aaggctttct tcatcggccg acaaccctgt ggcgggttcc ctactgaagc attaggtagc
     1381 cattgtgtat tttttagcta tcgaatatat acataaagag aaggtcggcc acggaggcct
     1441 accaatataa aaacatacag cgaggttaac atgagtactg ataaacagaa taatgataat
     1501 gcagcgattg caggtgccat tggtggagca gcaggtgcag caggatctgt tggttcagtt
     1561 gcagcagcag gaagtgtcgc aggattaggt gcaacaggaa tcacatcggg cttagctgct
     1621 attggaagta ttgtcggcgg tgggatggga gccggtctga tcgttacggc agcggctcct
     1681 gttgctgttg gtgccgctgc atacggctta tatcgctgga tcaaaaaata atatcccgct
     1741 catttcccta tcgttatttc ctggccgcag cctgaggcgg cctcttcctc ttctgaatct
     1801 gcctaccact cacattgaac ataacattcc tttaccgcag cgcatgaaaa agcccgcaaa
     1861 ttgcgggctt gagcaataat tttccagtat ttagtttgtg aagaacttct tcgccattgg
     1921 gtgatttttc atgatctcat cacgttcatc aggatcaaag catccgcaaa cgcccagtaa
     1981 gaccagttct gtaagcgttc gagcgcaaca acccgttgtt acctgcatct tccggttttt
     2041 gtcgtatcca aaaaccgtat acgtcacgta atcctcatca taatcctgct ggcaaataga
     2101 gcacgagtca ggatactttt tgatgagagc gttaatctgt tttatttgtg catcagtagc
     2161 cacaagttct ccgttagtac atccataact ggcattgaat gtaccacacc agaaaatccc
     2221 atagctgatt taacataaga ctacttaccc gcaccgcgcc ggaggcactc agggcgtttc
     2281 tgatggctga cgggcaaaag gtggtggcaa gtacaccgaa aaccaccaga aacgcacctg
     2341 atttatttta cgcatatgca tcacttattc acatcactgc atagccggtg atttcggtta
     2401 aaaaagactc tctgtgtgct gaaatcgcag aaaatggcca tgatccccgt cacgctcagg
     2461 ccacctgaga cgggcgttta tcagtctcca tactccctgt caccatgaga taatgtgcgt
     2521 tgccgttccg gtaccagatc tgctgtcttc tctggttcct tttccgttct ttgcccgtat
     2581 cgctgttgct caatctcatt cagcctgtcg ataaccggcg cagctgctcg ctcaaactct
     2641 gcacctgcct gctcaagccc gacacgcgct cgctcagcgc cgcgttctcc cgttgcataa
     2701 gaccagacat cttccgccat tcctcgaagg cgctctccca ctcgtccagc cttctcgagt
     2761 agtcctgctg tagctgctct aacgcgctca gcaattgttt ttccagttcc gtcattctgc
     2821 tctactcccg taccagcttc accgcgttcc gcgtcttgtt gtccaccgtc cggcccgttt
     2881 cggctttcat cccctccggc agcaccagaa accgtccgtt gcggttctcc agataggtca
     2941 cgccccacgt ctgcatctgc agtttcgaca gcgtatctct ctgctgcctg atttccgcca
     3001 ggttcgccgc tatccggttc ccctggtacc acagcacccc gctcaggctg gcgaacagca
     3061 gccctgacac catggtgatg gtcagccacg tccgcccgac cattcgcagc acgctcagcc
     3121 ggttcgactg catcgccgcg ttcatcccct gttcgtgggc gctgatggcg tcgttgattc
     3181 tcttcgcgct tgcgttcagt tccgatttga caacgttctc gtgcttgctg aatgcggctt
     3241 tcagcctctc ttcggtaagc tgctgctgtg ctttcgattt ctcctcgaag tccctcgagg
     3301 ccgttaaaag actgctcata aatggctccc ctcagtcgga tatttcgccc cccgtccggg
     3361 tcggcaatgc tgatactgtt ttttgtggtt ctgaccacct caaaaccggc cgctgtgagc
     3421 gcccctgtga cgtcatcacg cgttttcagc tctcctgatg aggctaacat cagcaaaccc
     3481 cgtgtaatcg tctctgcggc ttcctgcttc gtttctggca gtcctgacgg cgtgaccagc
     3541 gcccgccggt tctccgggtc gttcggatca tgtagcccca gcctgccgtt gaccactgtc
     3601 tgccacgcat cgatgcgcgg ccggtcggca cgatcgtaat acggctggag gcgtttcccc
     3661 gtcagtaatt cggtattggg gatcagaaaa ttcagctcaa gacgaccctt gtctgcgtgt
     3721 tccacccaca gaatgctgta ctgatcttta tcgagtccgg gcatcagaac ccgctcaaag
     3781 ctggccatca gtttttcacg ctgacccggc ggcaggtccg cctccgcgaa tgacagcacc
     3841 cccgaggtat attttttcac atacggcgac gcatcaatca gctcccggac ttcttccggc
     3901 ttcccctgca ggacgctggc accttcacgc tgccggtctt tacccagaag ataatccaca
     3961 ggaccggcgc ccccgccgcg accccgggga tggaatttaa caatcatcgt ccggcccttt
     4021 ttccagtacg gcatgccgca accgttccag accggcatcg atcgccatca gcgctgccac
     4081 gatctgcacc cggtcatgac cggcaccacc ggcgttcact ttccgggcga tctggttcag
     4141 gttattgccc atgccagcaa gctgacgcag cagcgcgggc gaaatcgagg gaagcctgcc
     4201 ggcgcgtgtc ggtttctcat caaggcaggt ctggcgcatc cacgccgcca gttgttttcc
     4261 gtcgcaccgc tcaagcaacc gccggtgttc gtcttccgtc acccacatcg tgagcatctt
     4321 gttgcgttta tctgccagaa tcgcctcctg ataacgccgt gacggacggg aaagcctttc
     4381 ccggtcggcg cggggttgga caagccgcag gcgcgtcagt ggcttttagc gggtttcggg
     4441 gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctaa atatgttaaa
     4501 ccgaagtggt gatttgccaa agtgcttcat gtggcaggag tgaaatgagg ctttccggcg
     4561 tcaggagaag atgctgggtc aggtgatatg ccgcttcctc gctcactgac tcgctgcgct
     4621 cggtcgttcg gctgcggcga gcggtaccag cttacaggcg gggcggaaat ttcctggtag
     4681 atgccaagaa gaagctttac ggagaagcga taaggccacg ccgaggccgt ttttccatag
     4741 gctccgcctc ccctgacaag catcacaaaa tctgacgctc aaatcagtgg cggcgaaacc
     4801 cgacaggact ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg
     4861 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
     4921 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     4981 cccgttcagt ccgactgctg cgccttatcc ggtaactatc gtcttgattc caacccggta
     5041 agacatgcaa aagcgccact ggcagcagcc actggtaact gatttagagg agatagtctt
     5101 gaagttatgc gccggttaag gctaaactaa acgaacagtt ttggtgactg tgctcctcca
     5161 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa aaccgccctg
     5221 cagggcggtt ttttcgttca aagaagacta cgctgtcctg taggtcaacg tcaagaaaca
     5281 aaaaaggccg ccttagcagc cttacttact gaggtgttta ggttcctcac ccctccctgg
     5341 ttacggtttc cctgggacta aaaatgacca tgcttcgtag ccgtaatcat gcgcatcaag
     5401 caatctcgtc gagtttttaa cccgacgata acggcagaat acccatttga agcctggtgg
     5461 tgcaggttta ttcaaaacgc agctcccatc aactaaaaat gccatccagt tattggctga
     5521 tgagagcaaa caagctacac ttcgattccc caatctaagt tttgctttat tttcccatca
     5581 gtttatgtgg ctggcggatg acttcgccac acaaaaaaaa tggaaagata agctacttgc
     5641 ttttcttgcc actcccattt ttagtcgggt caaccaaaac atatgattga cccgacttgg
     5701 gagttggcgg caaaggctta cctttcacag aagtcacttc cacacccgta tgcccaccac
     5761 gcggtccgac aatctcatat tgccccgaac gcatcgtaat atttcccggt ttcataatgc
     5821 cccctgtata gtattgtcaa aacgaactta ctcgtatcac ttcaacttga acaatacatt
     5881 gtcgctcacg gttcagtcaa gccctgacgg tacaaaaaag tatcgccacg gcgatacgtc
     5941 gtattgctat agcaatactt ttatttcata ctgcctgtaa ttaaccgtgc agaaaagcac
     6001 aaccacccaa cccataaaca caatttgaat aggaatatca tatgcttagc gaaaaaatca
     6061 gagaactgag gacatctcgc gggctgtcct tggatcaatt agcatcaaac gtaggttcta
     6121 caaaaagcta catttgggaa ttagaaaaaa aaccagggat aaaaccatct gcagagcttg
     6181 tttctaaact tgctcaagca ttagatgtta caatggactc tctgatggat gagaaaaaag
     6241 aaaacgacca agatatagtt ttctttagag aatacaaaag cttatcagaa gaaacaaaag
     6301 tgcaactgat gaatatattg cgagcaataa aataaggttc tagatgacta cacctaggct
     6361 ccaggcggca ctggtttcag aacagtggct tagaggaaag cacttcgctg atgctcttcc
     6421 tgttgattgc attaagatag ctgaaagcct aggaataaaa gtccaagggg cccctcttaa
     6481 ggatgaattc caaggggccc tctttataac ccctgatgta aaagccatta ttttcaatga
     6541 aaacataaaa gaagatgggc ggaaaaattt caccatagcc catgaaattg gtcactactg
     6601 tctacatagc gatagagagg aagttcaatg ccgattagag gacatgggag cattcgttct
     6661 aacaccgcat ggtaaggaga tagaaaaaga agccaatgag tttgcagctt gtctattgat
     6721 gccagaaaaa gatgtacagc agcaactctt agataaaaac ctcgatctca aactcgtgct
     6781 tggtctcact cttcggtatg gaacaacagt aactgcaaca gcttgtcaga tggtgcacgt
     6841 gtctgataaa ccacttgcag tcgttatgtt aaacgaggat aacacgacaa aatggtgtct
     6901 tcgtaattcg catttccgtg atttctttat caaaaaaggg actcttataa acatccaaga
     6961 acacttaccg gaggaactgt caccaacata tcaagacaca tggttttcaa gctctgtgaa
     7021 aagcgtgtgg aatctaaagg cttcatcttt agatatggag gaatacaaac aaagaatcta
     7081 tatgatttca ggcagtaagc ttacttaaaa ttattgttct cataatcagc gacagcctcc
     7141 cccaataaac ttaggcagac aggtctaccc tctacggtaa ccatgaaaaa aaccgtactt
     7201 taacacccac caatatattg tatttaccat aatcccttaa ttgtacgcac cgctaaaacg
     7261 gcctcagggc gtttctgacg ggaaaccggt aaaacaggta acaaaccacc cgaaaacccg
     7321 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcgcaa gtgcatagcg
     7381 cggaaattga caccattcag ccaggataag tcctgtttcc ggcgttattt ccatccgaaa
     7441 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     7501 accggttttc agcacgatca ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca
     7561 agtcgccgga cgactcacca cacgattcac cggcgactct tcactagagt cgtacccaga
     7621 gtcgccgagt ctaaaaaaca accaaacggg gagtcgggtc tagagtcggg tcgttattaa
     7681 ctcattgttt ttatagccct gagtcgtcac cgactctatg acgactcgcc ggggactcta
     7741 tgacgagtcg tcatagagtc ggatctatgt aaataaaatt taaaaaccgg aatttcagga
     7801 aggtaagcta ctgatattaa atacaaatct tcaaaatttt aattttgcgt agtgtgtggg
     7861 tatttttagt cgtgaggagc gttcagagat ctggcgattt ttgggaaggt tcaggaactc
     7921 cccgggatgg ccggagagga aaactcaagc atggcaggat caggattttt tccagaattt
     7981 ccaccatggt gccggggatt ccgttaattt tgcctggtga ttttcaagaa ggagcatggc
     8041 ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt aaggtgttca ggtgttcaat
     8101 ctcctgtttc agatgacgaa tcacctgttc actctggtca gcagaggctg tatcacgctg
     8161 ttcagagacc tgtccataat ggtgttcacc cttcggttgt tcaaagggac caaagacacg
     8221 taaaagctct gaagtatcta ccatttttgc attgcgttca ccgctacagg tggacaggtc
     8281 acctgcttta attaacctgt gcagtgtcgt tctactctta cccgtcagtc gggcagcctc
     8341 actaatagac actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt
     8401 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaagatcggc ctcgacatcg
     8461 agatcaagct cgttaagctt ctccagtagg agcagagact gattctggat gaatttagcc
     8521 atgttcagcg ctgttttctg ttgtttcgtc ataaggggcc tcagagcgtc agtgctcaat
     8581 gtagggatgt tctcttttcc gcttcctcgc tcactcagtc gctacgctcc tgccgttcgg
     8641 ctgcggcgag cggtcgcaac tcacgtataa gggcacgaga cgacagcaca gagagccaaa
     8701 gagaacaagg agaaggccgc ggcgttgccg tttttccata ggctccgccc ccctgacaag
     8761 catcacaaaa tctgacgctc aaatcagtgg cggcgaaacc cgacaggact ataaagatcc
     8821 caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgcc
//
