LOCUS       NZ_CP070090             1916 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1306 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP070090
VERSION     NZ_CP070090.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357448
            Assembly: GCF_016904295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1210.1x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,350
            Genes (coding)                    :: 5,228
            CDSs (with protein)               :: 5,228
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 61 of 122
            Pseudo Genes (incomplete)         :: 62 of 122
            Pseudo Genes (internal stop)      :: 21 of 122
            Pseudo Genes (multiple problems)  :: 19 of 122
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070090.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1306"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1306"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(169..471)
                     /locus_tag="I6K36_RS27155"
                     /old_locus_tag="I6K36_27165"
     CDS             complement(169..471)
                     /locus_tag="I6K36_RS27155"
                     /old_locus_tag="I6K36_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            1590..1826
                     /locus_tag="I6K36_RS27160"
                     /old_locus_tag="I6K36_27170"
     CDS             1590..1826
                     /locus_tag="I6K36_RS27160"
                     /old_locus_tag="I6K36_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1252..1425
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(782..1273)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 caggggcgtt tttccgggat actggtgagg aaaggtgctg gaaacggccc tgatgcagca
       61 ggattgcgct gagggcgcct gcgggggtgc gggcaaggga tgttatgttt aatcggtctg
      121 accgtcaggt acaaaaaaag cccttagggg gctttttttt gctatgagtt agccatattt
      181 tcgtgcgaat ttttcatcgg aatcgcatag ataaataata aactcaataa acgcgatgat
      241 cgcgggtatg aatgtccagc aaaaaattag atacaggaac ccctgtccta tttttcctaa
      301 gtaaaattta tgtgcgccaa atccaccaag gaagaatgct aatagcgcgg ctgcgattct
      361 acttttttca cctgaacctg aagaggcgtt tgtggctccg cagtgcgggc atgactttgc
      421 tgtttcgtgt atttccttgc cacatcctcg gcagtaaacc attgaactca ttttttatcc
      481 tcgagcttat tttaattggg atcataatat ccgatgtttt ataacgatgt gtatcctatg
      541 tgctcggatt ggatagattt ccattacggt gaaggatctt cttgagatcc tttctgtggc
      601 gtcgtaatct cttgctctgt aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt
      661 ctcagagcag caactctttg aaccgtggta actggcttac gaggagcgca gtaaccaaat
      721 ctgttctttc agtttagcct taacaggcgc ataacttcaa gactaactcc tctaaatcag
      781 ttaccagtgg ctgctgccag tggcgataag tcgtgtcttt ccgggttgga ctcaagatga
      841 tagttaccgg ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccatc
      901 caggagcgaa ctgccttcgc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca
      961 taacagcgga atgacaccgg taaaccgaaa ggcaggaatg cgggggagca cgagggagcc
     1021 accaggggga aacgcctggt atctttaagc cgcatcgggt ttcgccacca ctgatttgag
     1081 cgtcagattt catgatgttt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg
     1141 gccttatctc ttccctgtta agctccttcc tggtatctcc caggaaattt ccgccccgcc
     1201 tgtaagccgg caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1261 aagcggaata tatcctgtac tacatatcct gctgacgcgc tggtgccgcc ttttttctcc
     1321 tgctacatga agcacttcac tgatttccac atccgtgcca acatagtcag ccagtataca
     1381 ctctgctagc gctacgtgac tggttcaggg ctgcgccccg aaacccgcta aaagctaatg
     1441 acgcgcctgc ggcttgtcca acgcctcacg gcaccggatt aaaatccgct cccgttcacc
     1501 gtttctgact gcctttcacc gtcgctggcg cttcatcgtt tctactgtgt ctggcagact
     1561 gtgctgattt attctgactg gagaacgtta tggccagact gacaaacttg acgcctgcag
     1621 agaaaaaatt tcttgatgat gctgttgccg cagctgaaag agcgtcaggt aaaaagctaa
     1681 atcagcctaa ccggcacatc gtcctgaaca gagcgcgtgc gcaaattgag ttgcagcgtt
     1741 acgccgaccg ccagcgtgca ttacgtgagg atgagcggca gcagtccgat tttgcctggt
     1801 cacgtccgcg cgcgccgcgc cgctgatttt ggcttttggc ggtttagtgg tgctgacgct
     1861 gcgaaatcac ctgccattca cctgcgtgca tagccatgca gtcgcgcaga aatcat
//
