LOCUS       NZ_CP070085             5585 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1328 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP070085
VERSION     NZ_CP070085.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357470
            Assembly: GCF_016904275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 688.63x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,781
            CDSs (total)                      :: 5,656
            Genes (coding)                    :: 5,521
            CDSs (with protein)               :: 5,521
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 60 of 135
            Pseudo Genes (incomplete)         :: 79 of 135
            Pseudo Genes (internal stop)      :: 17 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070085.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1328"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1328"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            195..704
                     /locus_tag="I6K58_RS01405"
                     /old_locus_tag="I6K58_01405"
     CDS             195..704
                     /locus_tag="I6K58_RS01405"
                     /old_locus_tag="I6K58_01405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            711..923
                     /locus_tag="I6K58_RS01410"
                     /old_locus_tag="I6K58_01410"
     CDS             711..923
                     /locus_tag="I6K58_RS01410"
                     /old_locus_tag="I6K58_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1299..1427)
                     /locus_tag="I6K58_RS29050"
     CDS             complement(1299..1427)
                     /locus_tag="I6K58_RS29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1590..1814
                     /locus_tag="I6K58_RS01415"
                     /old_locus_tag="I6K58_01415"
     CDS             1590..1814
                     /locus_tag="I6K58_RS01415"
                     /old_locus_tag="I6K58_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1798..2064
                     /locus_tag="I6K58_RS01420"
                     /old_locus_tag="I6K58_01420"
     CDS             1798..2064
                     /locus_tag="I6K58_RS01420"
                     /old_locus_tag="I6K58_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2134..2484)
                     /locus_tag="I6K58_RS01425"
                     /old_locus_tag="I6K58_01425"
     CDS             complement(2134..2484)
                     /locus_tag="I6K58_RS01425"
                     /old_locus_tag="I6K58_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2550..3095)
                     /locus_tag="I6K58_RS01430"
                     /old_locus_tag="I6K58_01430"
     CDS             complement(2550..3095)
                     /locus_tag="I6K58_RS01430"
                     /old_locus_tag="I6K58_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3121..3447)
                     /locus_tag="I6K58_RS01435"
                     /old_locus_tag="I6K58_01435"
     CDS             complement(3121..3447)
                     /locus_tag="I6K58_RS01435"
                     /old_locus_tag="I6K58_01435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3809..3967)
                     /locus_tag="I6K58_RS29135"
     CDS             complement(3809..3967)
                     /locus_tag="I6K58_RS29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071557585.1"
                     /translation="MHEPPVQSDYHALSGNYRLESNPERHDKSPLAVATGNQELVDLDI
                     ESLEVVA"
                     /name="replication initiation protein"
     gene            4592..4768
                     /locus_tag="I6K58_RS01440"
                     /old_locus_tag="I6K58_01440"
     CDS             4592..4768
                     /locus_tag="I6K58_RS01440"
                     /old_locus_tag="I6K58_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4758..5102
                     /locus_tag="I6K58_RS01445"
                     /old_locus_tag="I6K58_01445"
     CDS             4758..5102
                     /locus_tag="I6K58_RS01445"
                     /old_locus_tag="I6K58_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5284..5529)
                     /locus_tag="I6K58_RS28775"
     CDS             complement(5284..5529)
                     /locus_tag="I6K58_RS28775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4330..4500
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="1.64e-38"
     Typing          complement(3859..4349)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.481"
                     /coverage="81.0"
                     /evalue="0.0"
     Typing          3..1039
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.711"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5093..5583
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.76e-102"
ORIGIN
        1 aacgaagcag gaagccgcag aggctattac gcggggctta ctggccctcg cctcgtcagg
       61 ggagcttaaa acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt ttgaggtcgt
      121 gcgcaccaca aagagcagca tcagcattgc cgacccggac ggagggcgaa acatccgact
      181 taagggagcc atctatgaac agtcttttaa cgctggcgaa ggacttagag cagaaatcga
      241 aagcgcagca gcagagtacc ggcgaaatgc tgaaagccgc attcagcgag cacgagaagt
      301 ctgtcagagc ggaactgagc gaaagcggga agagaatcag cgccgccatc cgcgcccacg
      361 agcagggtat gagcgcggcc atgcagtcga accgcctgag cgtgatgcgt atggtcgggc
      421 ggacatggct gaccatcacg atggtgtcag gactgctgtt cgccagcctg agcggggtgt
      481 tgtggtatca ggggagcctg atagcgtcaa atctggccga aatcgaccgg cagaacgcgg
      541 cgctgtcgaa gctgaacgcg aagacctggg gcgtgacgta cctggaagac agcaacggac
      601 gttttctggt gctgccgaag gggacggccg tagacaggac gcagagctgg acggtcggga
      661 acgggcggtc gaagcagaac gcgctgagac tggtgaagga gtaagtgccg atgaccgagc
      721 tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg aaaaggctgg
      781 acgagtggga gagcgccttc ggggaatggc ggaaaatgtc agggcttatg caacgggaga
      841 acgcggcgct gagcgagcgc gtgacgcgct tgagtcagca ggtggaacgc ttgagcgggc
      901 aactgcagcg tttgagccgg tagtgcagcg tcatgagatg gccgtggccg cagaacgggc
      961 gcacgagcaa cggcagcacg aaaaggaact gacggaagcg cgctcccggc agcgaagcta
     1021 tgacggtccg tcgctgggct gaaatgcagg aaaacagggt tctcatcggg catttacgct
     1081 gaaaccggat gtcattcacc tggctgcatg gctatgcagc caggtaaaat ttatctgtcg
     1141 cgaacggggc ggtttatcgg gtggtttgtt gccggtttta cctgactgcc accccgttcg
     1201 cggcgaaccc gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag cgtgaacggc
     1261 agatacaaaa aagccccgga gggcttttaa ctactgactt atttttttga agatttgctg
     1321 ggtttcaatt tgtcagtatt tcccggcgtt tttttattgt cacgctggcg tttatcaggt
     1381 tcccctgttg aatcggcaat tcgtttcgga cccggcttaa gacccataat cgttatcctt
     1441 tcaatagtta agaggaaagc cctcaagcta gatatattga ccgcgttacg gattatcaag
     1501 aggggctaac aagattattt tttgttcttt taggttgatt gtggtactgt tgatatacat
     1561 gtatagcaaa tggtgttcgg gaggtttata tgcttgctat ccggttatct gatgagattg
     1621 agtcccgtct ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt tatgcgcggg
     1681 aagcaatact ggcgcatctg gaagacctgg aggattatta tctttcagca gaaactgctg
     1741 cacgcgttcg ccgtggtgat gaagcagtgc attcgtctga agacgtgagg aagtcacttg
     1801 gtctggacga ttaactattc cgatcgggcg ctcaaatcgt tacgcaagat ggacaaacag
     1861 aacgcacgac ggattgtgga ttttatgagt ttacgcattg cagttgctgc cgatcctcgc
     1921 cagtcaggga agccgctcaa aggtgagctg ggcgagttct ggcgctatcg ggtgggagat
     1981 tatcgcgttc tgtgtgagat ccgagatgac gagcttgtta tccttgccgc cacgattgga
     2041 catcgccgcg aagtttacga ctgaagcccg ctatgcgggc ttttttgatg ccgacaacag
     2101 gtattatggt aaatactcga ctaactacgt gttctactgt ttgtcggttt tagttaacca
     2161 gcaggttgtc ttaacgataa tgtagccgtt atttttaact aataaggtca tattgttaac
     2221 gtcaggcata ataaaagaca ttatggcctc agagttatca tcagaagcag ctctctgcat
     2281 tactacttcg attccctttt ctttgaatct ataagtatcc agttgcctat taaacttaga
     2341 aagacctaca accatactta gtttgttatc tttaacagta agagatgctt cctgagtacc
     2401 attacatcgg tagtaagcgg tgtaaccgtc agcaaaagat cgaccgggga atataaatgc
     2461 taggaacgca attaaccaat acatagttga gaatcttggt attacacatt tcactatcct
     2521 ctctttcatt tttcgttcac catactttgt tatctgattt ttaatttttc tattttataa
     2581 accaagtccc taccaaaaag gcgaaaatta tcctcaaata ataataattg tttaagaaca
     2641 tccatgaaag cagctgcttc accctcagtt atgggagcta ctctatataa catataacct
     2701 gagttaaggg tttcttttat actttcttca atgctcatta taaatattag tggattatca
     2761 tttttcttcc cccatgggtt tctaattaat ccttcgatgt actcaggtcc tttgcgtcct
     2821 attgcctgcg caatgttttt tatttcatct gcgtgagggc tttgttgtac agctttgttt
     2881 atgcctgctt gagtgatttt cagagactct acaccgtgta tgttgtatga gataaaatgt
     2941 aataaattaa gatgtaatgc aattaatcca tttttgttta catctgagta tttggataaa
     3001 aaacgtaatg aagtgttaac tctcccttga agcctaacaa ttgggagtgc taccttgtat
     3061 atttccttct ccgttaaata ccgtttaaaa aacataattc tccgcctccg tatagcgtgc
     3121 ctattttatt tcgtcgtaaa acttagctct gaaatattcc tttagaacca tcatttcatt
     3181 aataattgaa tctatctcat ccattttttc ttggtattct tttaatggtg aagggagcgg
     3241 taactcaata tttgaaaagt gattggcgag atagggatca aggtttctca tccattcagg
     3301 cttttcgcca atgcgttctt caactgaaag tgaacctgat aatctctggt taataagatc
     3361 acttagactc attttggttt tattcgaaag ccgttgtaag gattgcttta atcctatatc
     3421 tagcatgata tttgttcttt ctctcattac cccccccctc gctattgcgt tttttgttgc
     3481 gcacaaagct accattgcgc attaagaaac gcaataaaaa aggagggagg gagtggtttg
     3541 tacatgatgt gcagtgcgct gagggcatca gtggcgggtg tttttccgtg ggatggatgg
     3601 agctccgggg ataacagaac tgacgatcat ttaaaggatc ttcttgagat cctttcagga
     3661 gcgtcgtaat ctcttgctct gaaaacgaaa aaaccaccct ggcaggtggc tttttcaaag
     3721 gttaaaaaat cctggcagat cttttaacca aggtaactgg ctttagtgga gcgcagatac
     3781 caaatctgtc ctttcagtgt agccgcagtc aggccaccac ttcaagactc tcgatatcta
     3841 aatccactaa ttcctggtta ccagtggcta ccgccaatgg cgatttgtcg tgcctttctg
     3901 ggttggactc aagacgatag ttaccggaaa gagcgtggta gtcggactga acggggggtt
     3961 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     4021 tgagataaac gcggccataa cagcggaatg acaccggtaa accgaatggc aggaacagga
     4081 gagcgcacga gggagctacc ggggggaaac gcctgggatc tttatagtcc tgtcgggttt
     4141 cgccgccact gatttgagtg tcatatttcg tgatgcttgt caggggggcg gagcctatgg
     4201 aaaaacggct tcgccgcggc cttctcattt ccctgttaag ttccttcctg gcatttacca
     4261 ggaaacttcc acccaattcg taagcccgca ccgctcgccg cagccgaacg accgagcgca
     4321 gcgagtcagt gagcgaggaa gcggaatatc tcctgtattg catcttctgc tgacgcgccg
     4381 gtgccacctt tttctcctgc catatgaagc actttcataa agtgctactc cgccgtaaca
     4441 tattgagcca gtatacacag cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc
     4501 cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg cgcgtcccgg aaaacatttc
     4561 cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga agccgctcgt
     4621 cgtaaaaaaa agatcctggc atccggcagt gatggcaaat ctgatgcgtc aggcacagga
     4681 ggaagattac cgccgggagc agatggcaga gcttgagcgt tcagtccggt cacattttgc
     4741 ggatatcgga ggagaggatg gcggttaagc gtacccgttt tttaggtatc cgggtgaccg
     4801 atggggagta ccagcagctg ctggagcgct gcaatggaag gcaactggcg gtgtggatgc
     4861 gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg
     4921 ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc cggaaaatta
     4981 acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg atggccatcg
     5041 atgccggact cgagcggctg cggcatgccg tgctggaaaa gggggcagac gatgatcgtt
     5101 aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct gctggggaaa
     5161 gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt ccgggagctt
     5221 atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt tgctgaacag
     5281 gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt tctgatgccc
     5341 ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa ggggcggctg
     5401 gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac
     5461 tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg
     5521 gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc atcttcgtta
     5581 cccaa
//
