LOCUS       NZ_CP070081             2101 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1371 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP070081
VERSION     NZ_CP070081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357513
            Assembly: GCF_016904255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 751.4x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,937
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 68 of 181
            Pseudo Genes (incomplete)         :: 109 of 181
            Pseudo Genes (internal stop)      :: 39 of 181
            Pseudo Genes (multiple problems)  :: 34 of 181
            Pseudo Genes (short protein)      :: 4 of 181
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1371"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1371"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(1299..2101,1..163)
                     /locus_tag="I6L01_RS24425"
                     /old_locus_tag="I6L01_24430"
     CDS             join(1299..2101,1..163)
                     /locus_tag="I6L01_RS24425"
                     /old_locus_tag="I6L01_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            167..433
                     /locus_tag="I6L01_RS24430"
                     /old_locus_tag="I6L01_24435"
     CDS             167..433
                     /locus_tag="I6L01_RS24430"
                     /old_locus_tag="I6L01_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1693..1924
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ggcgctaaaa aacgtcctcc agttggatcg agaaaccaat gaggatcttg tcattgccga
       61 cgatgtaggg gatggcactg atgacgggaa gcggacggcg tttgtctggg attcaggtaa
      121 acggcgttac aaacgcgccc ctgagaagga taaatcggat taacgtatga atattaatat
      181 tgaatacctg aatggaaata agactattgg tttatttttt ttaagaagtg aagcggtgat
      241 tcctgacagg tttaaaaacc ttattttgct tattgatgga ttaagttttg gcacatttgg
      301 ttttcatccg cacgaaggtt ttgaggatga attaatttta tatattcaga aaacaaacga
      361 gagggtaaaa actctttttg tgaaaataga tttaaacggt atcaagtttg gatttttaag
      421 aacgcattct tagttctgga aaagagccag cggcaggctg aggtgatagg tacgagattg
      481 catgcaatct ctagtgctct gtctatcctg cattatcctc agcattatcc tcagcattat
      541 cctcagcctt gccaactcga caccaatgca ggatagacaa tccgatgtca aatgttaaca
      601 ctctgcgagt ggtacatttt ccccggatta tcgtcctgag cctgccgctg gctctctttc
      661 taccgcctcg ctttgctcgt tgctcaacgc ctcacagaca cggattaaaa tccgcatccg
      721 ttcaccgttt tttaaagtcc gttaaaagca tgatgccatc tccgagagtt aatctcgtca
      781 aatgctaaat cgtgggggtc ccctttgggg ttccgattta gtgattgacg acaccaccga
      841 ttaaaaaact tatgcggggt ggatggtttc acgaagtgag gccatccacc tgtaagacag
      901 ggttttgttt ttattccctg ttttggtgat cgggtgtgtg gaaaaggttg gggtaagccg
      961 ttcgggggtg cttgttttgg ggggttaaaa ttgtggttat tttttgcgca attctcgcgc
     1021 gtgatccttg tatttatact taagggataa atggcggata tgaaatagtg gtttagccca
     1081 gtaatgacga ggctttgagt gggttttgac aggtcaaaga aaatggagca gaattgaggc
     1141 gtttttaatc ggcgttgggg agtgcgtcaa cactccccaa catttcgaat gtgtcacctc
     1201 agcggcaaac tctggtgaca tgtactggct cgcaatgcac aggtacgtga tgaatatacc
     1261 acatcaaatc acagcctgcc cagatcggag caggcttaat gtcagaagat aaattccttt
     1321 cggactacag cccccgtgat gcagtttggg atacccagcg cacgcttacc gattctgtcg
     1381 ggggtatcta ccagactgct gctgaattcg agcgctatgc actccgtatg gcctcctgta
     1441 gcggtttgtt acgttttggt tggtctacca tcatggaaac cggagaaacg cgcctacggc
     1501 ttcgtagtgc gcaattttgc cgtgtccgtc attgccctgt ctgccagtgg agaagaaccc
     1561 tcatgtggca agcccgtttt tatcaggctc taccgaaaat cgttgtggat tacccgtctt
     1621 cccgatggtt gtttctgacg ttaactgtca ggaactgcga gataggtgaa cttggaacag
     1681 tccttacagc aatgaatgcg gcgtttaagc gaatggaaaa gcgaaaggag ctatcacctg
     1741 ttcaggggtg gatcagggct acggaggtga cgcgaggtaa ggatggcagc gcacatccgc
     1801 attttcactg tctgctgatg gtgcaacctt cttggtttaa agggaagaac tacgttaagc
     1861 acgaacgttg ggtagaactc tggcgcgatt gcttgcgggt gaactatgag ccgaatatcg
     1921 atattcgggc agtaaaaact aagacaggtg aggttgtggc caacgttgcc gagcaactgc
     1981 aaagcgcggt tgctgaaacg ctgaaatact ccgttaaacc ggaagatatg gcaaacgatc
     2041 ctgagtggtt tcttgagctg acgcggcagc ttcacaagcg ccgttttatc tcgaccggtg
     2101 g
//
