LOCUS       NZ_CP070080             5164 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1371 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP070080
VERSION     NZ_CP070080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357513
            Assembly: GCF_016904255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 751.4x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,937
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 68 of 181
            Pseudo Genes (incomplete)         :: 109 of 181
            Pseudo Genes (internal stop)      :: 39 of 181
            Pseudo Genes (multiple problems)  :: 34 of 181
            Pseudo Genes (short protein)      :: 4 of 181
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1371"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1371"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(4444..5164,1..206)
                     /locus_tag="I6L01_RS24400"
                     /old_locus_tag="I6L01_24405"
     CDS             join(4444..5164,1..206)
                     /locus_tag="I6L01_RS24400"
                     /old_locus_tag="I6L01_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_025732090.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     FTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            209..742
                     /locus_tag="I6L01_RS24405"
                     /old_locus_tag="I6L01_24410"
     CDS             209..742
                     /locus_tag="I6L01_RS24405"
                     /old_locus_tag="I6L01_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(808..1017)
                     /locus_tag="I6L01_RS24410"
                     /old_locus_tag="I6L01_24415"
     CDS             complement(808..1017)
                     /locus_tag="I6L01_RS24410"
                     /old_locus_tag="I6L01_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            2751..3665
                     /locus_tag="I6L01_RS24415"
                     /old_locus_tag="I6L01_24420"
     CDS             2751..3665
                     /locus_tag="I6L01_RS24415"
                     /old_locus_tag="I6L01_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(3869..4267)
                     /locus_tag="I6L01_RS24420"
                     /old_locus_tag="I6L01_24425"
     CDS             complement(3869..4267)
                     /locus_tag="I6L01_RS24420"
                     /old_locus_tag="I6L01_24425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          2752..3665
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4445..5163
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.333"
                     /coverage="99.0"
                     /evalue="8.55e-160"
ORIGIN
        1 gccatcttga acaggcgcga acggtcgggc atgacatcag ggaatggctg gacactccgg
       61 ttaagcaaca tgaactggtc agaaggctgc aacatgatgt tgcgcgggag catggctttg
      121 acatgagaga cagagtgagc agacagaacc gaactgcacc tgaacgggtt aaatcaccgt
      181 cacgtggatt ttctatggga ttctgaacat ggccagcaca gaagaaaaat tactcacgct
      241 cctgttatcg gtggagcatc tgcaacatac cgcaatggag caacagaaag ctgttgctga
      301 ttctctggct gtggcaaggg aaactcttcg cggatatcgg gatatttctg acggtgtggc
      361 aaaacaggtc agggatgaag tcagaaatga ggtgcagaag ctggacgtgg caggactgat
      421 aggtgagcgc atcagccagg attttcagcg gctggaagag tcagtcgcaa acatgaaggc
      481 aaacgcggat gccctgaaca gggatattgc gacaattcac agcaatatcg tgcaggaata
      541 caacaccctt aaagggtata gctggaaatg gctgggtggg gtgtcagtgg cttttctggt
      601 tatgttctgt gtgctcggat ggggaatgaa aacaatactc gacaggaact acaacgccac
      661 agcggcggtg tatcagaaag ttgtgacact ggaagataaa ctggtggaga aacctgtcac
      721 ggggaagagc cggaaaaact gacgcgcttc gcttgtccaa cgcctcacga cccgcgaaag
      781 gatttcgcga accgttcacc gtttttttca atatgattct gtctgctttg gtcgataaag
      841 cagacgatag tattccagcc ttcgtatata taacggtacg tgctcatcag taagagctgg
      901 cggtattgtg ttatcagggg gaagtttttt cagatactca cgaaatgcct ggcttgatgc
      961 cataaaatct acagtagcat cgataatatg tggggtgttt tcacctaatt ttgccatgat
     1021 gataatcctt atattatcac ggtttgtgaa tgatagggtg tcactattat ttatgcgtta
     1081 gtgaaccaga aaaaatcccc cgttggcata gggggatatg taaggagaaa taacttcgca
     1141 ggtgttcttg tttgtataac gtgaaaagac tgcacgcaac ttaataagtc tgccgtactt
     1201 actttcagtt ttcaacataa ggtttataac aatgtatgga atggaagtga gctttaaggt
     1261 ttcagctcta aaaaataaaa tatttattag ttggctggtt gttgttcttg ttctgtatgt
     1321 atataaacac tatcgacata ttatgaccgg gaacattcct taccccctct gcgctcgcag
     1381 aggttttttt tgtcattaat taataatgaa ctgaaatgca gttccgatac ctccatttgc
     1441 acattatccc tgacatatgg taccggaacc gcattaattt tatgttataa gagtttattt
     1501 ttaatgtcca gatggtttaa tagtatttat atgtaattag ctgtatggat agtactggtt
     1561 ttattgtata acttgtcgtt ttttttattg tttttgtgtg gtgtgctaat ctttaagtta
     1621 taaaaaaacc ccttggaata caggggcaaa agtaaggaaa acaaatggat gctcagtttg
     1681 tgtgacactt taaactaatg gaactataga gtggaggttg ttacagtcgc ttttataaaa
     1741 cagcaaccag tttttttcaa tagtctgatg atggttgtga cagttttgtg atattgttca
     1801 aaatgtttct tgttgaatgt ttgttttttg gtctattggt gtctggtttt attttttctg
     1861 ctattacata tcaagaaatg tgattggtgt agcaagagtg gaatgcgatg tggataaact
     1921 aattggttgt cgaatctaat ttacttagaa aactacactt gtttttgtgt atgaatttag
     1981 tgaatttgtc tgtctgtgtg ttttcgtgca tttataaatc cttcgtcagg atcttaaatt
     2041 tagtgttgaa tatgtagggc ctgtacggta attattattt agcgtagcta cttcatcgtt
     2101 gttcgaacac aaccaacaac ctgtaattgt tgtttctctt ggttccccac ttttgtgggg
     2161 ttttttatct cttccgtgca tcgtaaagcg cggactcctt ctggaaaggt tgttttaggg
     2221 catcactgag gcattttttg cgtttgtgaa tattgagcat ccgataacta aaatatttcc
     2281 catagactct gattcttgcc accgacccca ggtcggtttt tttttgtcct ggcgagatgt
     2341 gaagtttgga aacaggaaac cccgtcagag cgccacgcac caggggagaa aacttgtcgt
     2401 tttgagccgt tcggtgttca gaacgcacga aaccgatcgc acatcgcttt cgtgcatagt
     2461 tatgcaacgc cctgaaaacg attctgacgc gtttttctgg tttggcctgg tgttttcttg
     2521 tctttttgcg ttttttgcgc cagaaagcga ttgatggcgt tttacatggt acgtacaacg
     2581 ggagttatgg taaatggatc ggtttttcgg gaaggatcga caggatttgc cgttgggtgt
     2641 agtgtaagcg actgaaaaac aaacgcccct gaaattcgtg tctatccgcc aagactttca
     2701 cgaaaataca ggggcgatgt cggtcgataa cctggaagga atcgtaacac atgagcgccg
     2761 cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat gatctcgctt
     2821 tcggccttcg tatctccggc aaagggcgcg cgcttcttgc acggtatatc cagcagaacc
     2881 agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggct gcgattgact
     2941 ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg aacggacacg
     3001 ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc tcggttaagg
     3061 cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt gcggatgtga
     3121 actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg atggagtggc
     3181 gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc gcctcagagc
     3241 gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc gaaatgacgc
     3301 gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag tggcttgaag
     3361 cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt tcactggctg
     3421 aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc acgccggaaa
     3481 ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca cgcggtcgca
     3541 agggcggttc taagtctaag cgcagcacag tagctacatc tgcacgcacg ctgaaaccgt
     3601 gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga ggtctcgtag
     3661 agtagaccaa ataagcctat atcagataac agcgcctttc tggcgtcttt ttgagcagca
     3721 ggttttttgc cgtttttccc cgttcagccc tgcaatggcg cggctttccg cgtgattgtg
     3781 gtggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc gaggaagcac
     3841 cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat gtctccagat
     3901 cgtacttaag ttccttaagt gcttgtaata tcctgtcttt tttatagcat agctcgcctg
     3961 tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag agccggtgat
     4021 tgcgttcccg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt tttcgttcgc
     4081 tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggaga agtttgcgtt
     4141 cctgttctgt gagtccggct tcgttgtcga tcaggtcaat aatctgtctc tgtcgtgcgg
     4201 tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg atgttgctga
     4261 tgcgcatgtt cgtgatgctc cctggtgttt ttttgcggtg tgttgtggtc gtgcagtata
     4321 tcgcttgcgg tcggcgggtg cagggggtat cttccgtgct aattcgtaaa attagaagga
     4381 agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgca gagggctgaa
     4441 accgtggcaa cctatcacct cagcgttaaa tttggtggca aggggcaggc tgcaaaccac
     4501 gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc aggatttgga atattccgca
     4561 cacggaaaca tgccggaatg ggccagggat aatccttccc atttctggca ggcggctgac
     4621 cagtttgaac gtgcgaacgg ttcgacctat agggaacttg aaattgcatt accccgtgaa
     4681 ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga agctggcgaa
     4741 cggcacgcct ggagttttgc cattcacaat ccgaaagcga gcattgacgg cggcgaacag
     4801 cctcatgcgc atatcatgat gagtcagcgt gtgaatgatg gcattgaacg tacaccggaa
     4861 cagtatttcc ggcgctataa cgccaggtat cccgagcggg gaggtgcgaa aaaggattcc
     4921 ggaagcctga ctttcactca gcaaaaagag cagttgcggg aattgcgaaa acgctgggag
     4981 gtgaagcaca acgaacatat gagaaagcat ggctttgaaa ggggcttcat tgactgccgg
     5041 acgctgaaag aacagggcat tgaacgacgt cccgaagtgc atctgggttt tgaagctgcc
     5101 ggacggcttg atgatgccca gcgtcatgac atcatgcaaa aacggtctga accggactat
     5161 tccc
//
