LOCUS       NZ_CP070056             1013 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed8.
ACCESSION   NZ_CP070056
VERSION     NZ_CP070056.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070056.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..695)
                     /locus_tag="I6K20_RS25015"
                     /old_locus_tag="I6K20_25020"
     CDS             complement(<1..695)
                     /locus_tag="I6K20_RS25015"
                     /old_locus_tag="I6K20_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063268940.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMT"
                     /name="replication initiation protein"
     Typing          complement(2..695)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcatcttga tctgtttgtc ttccttgaag cgaaaatgca gggctacaac cgtgcgcccc
       61 tttttgatcg tctcaacagt tacaaccaga tcgctttttt cgttcagttc ggcgatggct
      121 ggcttgatta tcatcttatt caagtctttg aattgttgat atttatcttc aacggcaagc
      181 atggttcgga aatcatcgat agctatcgtt ctgtcaccgg tgctgcggaa ctgctggagc
      241 agctcgtaaa tgcggatgct gtaggtgctg gaaaggctgg agacgttctt cacgactacc
      301 cgcgtaaact gccccttgag ctgtgtcaga tacggcatga tggcatcgga gaaggtgata
      361 tctactttcg cctcaccttt gaaatactgc gcccggtact gcacccagcg gaactcttct
      421 cgtttttcct cgttcttgat gattactgac ctgtcccaga ggcgatctat cgcttccttg
      481 agcctagctt cagcgtgctg ccgtcccatg tcaggaaagg catcatgaaa ctctgcagcc
      541 gtgatggtca tggttttttg ttcatccggc gaaggggaat cttctccgga ttttaggcgg
      601 ctgatacaga ccagcaggaa gcgctgttcc tgcaaagtga gcttgtagct tgcctcgata
      661 agggcgtttg atttgtaagc tatcagatct gacatttcgc ttgctcgttg ctattggtgg
      721 cgttttttta ctgtacgcca cttatcatat gggtggaagt tttttttttg caaccccctt
      781 tttctgtgga ttacaccccc aaaagctcca cctttcaccc ccaaaagctc cacctttcac
      841 ccccaaaagc tccacttatc ttgccagaac atctttaaaa tcatggtgtt acaagaccta
      901 aaaaggtttt aaaaaaaaga tctaaaaata agatcttaaa aacttaacgg cgagcctgtg
      961 gataacttgg ggtgctgaaa acaaaaaacc aataaaaaca atgcgttgca aga
//
