LOCUS       NZ_CP070055             1021 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed7.
ACCESSION   NZ_CP070055
VERSION     NZ_CP070055.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070055.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(218..541)
                     /gene="mobA"
                     /locus_tag="I6K20_RS25005"
                     /old_locus_tag="I6K20_25010"
     CDS             complement(218..541)
                     /gene="mobA"
                     /locus_tag="I6K20_RS25005"
                     /old_locus_tag="I6K20_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            826..1020
                     /locus_tag="I6K20_RS25010"
                     /old_locus_tag="I6K20_25015"
     CDS             826..1020
                     /locus_tag="I6K20_RS25010"
                     /old_locus_tag="I6K20_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041645.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(551..789)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.917"
                     /coverage="99.0"
                     /evalue="5.9899999999999996e-114"
     Typing          complement(222..541)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.48e-67"
ORIGIN
        1 taccataatc ccttaattgt acgcaccact gaaacgcgct gaacgcgatt ctgacgcaga
       61 caccgcaaaa agagatgttt taccccggaa aaacaggaag gatgcgtcag gatcgttttc
      121 agctcgctgc atagctatgc atgaaagcga atggtgatca ctttgggagc ttacggtgtt
      181 cataccgtct gttttcgaca gttttctctc cgggaagcta atctgccata agcctggata
      241 acagggcacg gtgatactcc gtaatagcga tcagcacctc cgcatactcc ctgtccccga
      301 cacgcttgcc gtcgataaag agttttttct gcaacgcccc cagtcgcata acttctttca
      361 gcacccggtc atcagtcagt gagttaactt tcttaccgag agctgccgcc cgtaaaaaac
      421 cagaaacggt cttgccgcat tcagcagccc tttttctgat ttcctggtct tcttctgccg
      481 tcagacgaac agggcgactg atagcccgcc tgcgcgtatt acttccactt cgttttgtca
      541 taaaacatgg ctccgtatct gacatagggt gtcggggcga agccctgacc agggtaattg
      601 taatagcgtg catgtatgcg cggtataaca attgcacatc ctgtcctgtc agcaagctcg
      661 aattaccgac agaggcacgg cagttttttg gcagtgacag tattgtcagg cagaaatacc
      721 cgtcaaagcg tcagccggca gaataccgac ggtaaaacag tcgaaaagtg gtgcgcgata
      781 taatcacacg acgcagatga cagacagaac ggagaaaaca gaaagatgaa caagcagcag
      841 caaactgtac tcaacatggc gggattcata aaaagccaga gcctaacact gctcgaaaag
      901 ctggacgcac tcgatgctga cgaacaggct accatgtgtg agaagctgca cgaactcgca
      961 gaagaactcc agaacagcat ccagacacgc tttgaagtag aaaatagcac agagagataa
     1021 c
//
