LOCUS       NZ_CP070054             1025 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed6.
ACCESSION   NZ_CP070054
VERSION     NZ_CP070054.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..686)
                     /locus_tag="I6K20_RS25000"
                     /old_locus_tag="I6K20_25005"
     CDS             complement(<1..686)
                     /locus_tag="I6K20_RS25000"
                     /old_locus_tag="I6K20_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_097706625.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMT"
                     /name="replication initiation protein"
     Typing          complement(2..686)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.924"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcatcttga tctgtttgtc ttccttgaag cgaaaatgca gggctacaac cgtgcgcccc
       61 tttttgatcg tctcaaccgt tacagccagg tcgctttttt tgttcagttc gtcaacacag
      121 ggttttatta gtattttatt taagtctctg aattgtttgt atttattttc aatccctaac
      181 attgaacgaa agtcattaag agcaataatt ctctctcccg tactgcggaa ttgctggagg
      241 aactcgtata ttcttatact gtaactactt gataaacctg atatgttttt tataacaacg
      301 cgcgtgaatt gccccttcag ttgcgtcaga taaggcatta ccgcgtcaga aaaggttatt
      361 cgagccttgg cctcaccttt agcgtactgt gcccgatact ggatccagcg gaactcctca
      421 cgcttctcgt cattcttgag aatgatcgac ctgtcccata aacgatcgat cgcttcctgt
      481 aactgaacct cggcattttt ccgccccatg tcagggaatg aatcaaaata ttccgcagcg
      541 gtaatggtca ttgtcttttg caattcagca tcagcaccag atttcaatcg actgatacac
      601 agcaacagaa aacgttgttc ctgtagggtc aacttgtagc tggcctcaac caaagcattc
      661 gacttgtagg caatcaagtc cgacatacaa agatcccgtt caaatgtggt gatgctttaa
      721 tgtgccccac aaataagaaa tgtggaatat ttttttttat atcctcacat ttctgtgaat
      781 aacaccttaa attcctccac ctttacctta aattcctcca cctaaacacc ttaaattcct
      841 ccacctttac cttaaattcc tccacatata cctctataac atattgattt tattaatgaa
      901 ttttgaccct aaaacaagta ttaaaaacaa gatcttaaaa acaagatcta aaaacttaac
      961 ggcgagcctg tggataactt ggggcgctga aaacaaaaaa ccaataaaaa caatgtgttg
     1021 caaga
//
