LOCUS       NZ_CP070053             3201 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed5.
ACCESSION   NZ_CP070053
VERSION     NZ_CP070053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..301
                     /locus_tag="I6K20_RS24985"
                     /old_locus_tag="I6K20_24990"
     CDS             <1..301
                     /locus_tag="I6K20_RS24985"
                     /old_locus_tag="I6K20_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204693241.1"
                     /translation="KNLTASLWITWGAENKKPIKTMCCKKAELQVPNTPLPTLWKSSIH
                     AGSAHITYRKTVRMCSTWRRIHFRFLAHSLANARSLGLRRADDTITVLAAPPAR"
                     /name="hypothetical protein"
     gene            complement(270..524)
                     /gene="mobC"
                     /locus_tag="I6K20_RS24990"
                     /old_locus_tag="I6K20_24995"
     CDS             complement(270..524)
                     /gene="mobC"
                     /locus_tag="I6K20_RS24990"
                     /old_locus_tag="I6K20_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            703..2862
                     /gene="mobQ"
                     /locus_tag="I6K20_RS24995"
                     /old_locus_tag="I6K20_25000"
     CDS             703..2862
                     /gene="mobQ"
                     /locus_tag="I6K20_RS24995"
                     /old_locus_tag="I6K20_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(526..702)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          703..1061
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          617..2859
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaaactta acggcgagcc tgtggataac ttggggcgct gaaaacaaaa aaccaataaa
       61 aacaatgtgt tgcaagaaag cagaattgca agttccgaat actccccttc caaccctgtg
      121 gaaatccagt attcatgcgg gttcagcgca tatcacctac cgaaaaaccg tgcgtatgtg
      181 ctcaacctgg cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc
      241 gctggggctg cggcgagcgg acgatacaat cactgtcctg gcagcgcctc cagctcgtta
      301 atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg
      361 agtaaccagg cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca
      421 cccacaacaa ccttctgcgc tgtgtccagg ctccggctct tcgccttcag gcgctgtaat
      481 ctcgcctcag catcagcaat cttctgttcg agcgttctgc tcatgatgta actccgtgcg
      541 cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc
      601 gcgacaaatc acaaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc
      661 tgcggggctg aaagaaaact acaaaaaggc attacggcag aaatggcgat ttatcatctc
      721 agcatgaaaa ttatctccag aagcagcggc tacagcgccg tagcttccgc tgcctatcgt
      781 tccggttcac tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc
      841 ggtgtggctg aagctgtgat tctgaccccc gcgacagccc ctgcctggtg tacgaaccgt
      901 gccgaactct ggaatgccgt tgagaaggcc gaatgccgga agaactcaca acttgcgcgg
      961 gaaattgaac tcgctatccc ccgtgaactg ccgcaggatg ccgcacggga aaccgttctc
     1021 gcctttgtca gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac
     1081 atggataaaa caaatcccca tgcgcacatc atgctgacca cgagggctgt cggacctgct
     1141 gggttcggcg gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca
     1201 tcatgggctg accatgcaaa cagagcactg gcgaacgccg gttatcagga agagatagac
     1261 catcgttcat acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct
     1321 gcctgtgcga tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt
     1381 aacaggctta acctggaaat acagatttcc cgcactgaac tcaggaacag cgggctgaca
     1441 gagcctgccc gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg
     1501 tacacactac gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc
     1561 accagtaatg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag
     1621 ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg
     1681 ttttcgtccg gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc
     1741 gaacagggaa aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg
     1801 aaggtggaga aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag
     1861 tggggggcat ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg
     1921 cgccggaagg agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc
     1981 aggaaaaatg acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat
     2041 ggcgaggccc ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg
     2101 aacggagaat acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag
     2161 cgtgttgatt taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa
     2221 catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt
     2281 tgccagacat ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct
     2341 ttctgtcttg gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat
     2401 tttacgcagg gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa
     2461 acccgccagc ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc
     2521 cggcttaatg gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg
     2581 gtcatcagtg cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat
     2641 cacgatgcag acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc
     2701 cgtgaggcac cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc
     2761 cgctctctgg gtttcaatcg tacctgggat gaatggcggc gcgaggaaga tgcaagaaca
     2821 cacaccaccg agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt
     2881 tttgtttaac atcgtgttat gttcgtcgat gttcttctat gttcatcgac agggatgaat
     2941 gcaagaagca cacacataat gcaagcatag ttttgttttt ccgcccccaa aaattggggg
     3001 ttcttttttc gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg
     3061 cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgatttta
     3121 gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg
     3181 cccctttttg atcgtctcaa c
//
