LOCUS       NZ_CP070052             2042 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP070052
VERSION     NZ_CP070052.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            198..629
                     /locus_tag="I6K20_RS24975"
                     /old_locus_tag="I6K20_24980"
     CDS             198..629
                     /locus_tag="I6K20_RS24975"
                     /old_locus_tag="I6K20_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_024183523.1"
                     /translation="MRRITQVDQSTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQIDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="lipoprotein"
     gene            complement(693..1709)
                     /gene="mobV"
                     /locus_tag="I6K20_RS24980"
                     /old_locus_tag="I6K20_24985"
     CDS             complement(693..1709)
                     /gene="mobV"
                     /locus_tag="I6K20_RS24980"
                     /old_locus_tag="I6K20_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_204652620.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLENKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAVAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGFSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     Typing          complement(697..1709)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.746"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acatagaaat tcggacactc aatactggca gaaaaacacc tgattttgcc gatgtatgag
       61 tacggagagt aataagatac agaaggggat ctctcggaag ccgcgccaca agcggaaagt
      121 ttttgacaat agttatctct agagataact tagttatctt gagagataac tattttttcc
      181 cgaatggaga tttttagatg cggagaatca cgcaggtaga ccaaagtaca ggggaggaat
      241 taggtggttt tgtcgcagtg attcgtccta aacaaaagtc atcattccag aggcacttta
      301 ctatgaacca agccgcgcta attaccattg ccaatgagct aaaccatgac cagatgcgtg
      361 tgctgatggc cttgctcgca gaactggatt acgaaaacta catccaagtt gcgcagatag
      421 atattgcgga agcgctgact atgcaaaaaa caaatgtcag tcgtgcggta aaaaacctga
      481 ttgatttcgg gatcatactg gaaggaccga aaattgggcg gagcaaaacg taccggctaa
      541 atccccaatt cggctggaaa ggtactgtca gcaaccacaa gaaagcacta aaaaacggcc
      601 tcagcgttat acagggcggc agaacctgat ctctggaatg ccgcaaatga cctcgttttg
      661 cggcgtcaga cctatcctgc atcgcatcaa aactaacgac tcataccacc cataccacgc
      721 gatcgcgtct gtcgctgttt ttcacgttca atacgctgct gtttttcctt ttcctggttt
      781 tgctgcttaa accgctgagc catcatcagg atttcggtca tctgctctct ggaaaagccc
      841 ttaaaaggct ctgtaacgct ctgtaggcgt ttctgctgcg aatccatagt cttctgtagc
      901 tcgcgcgctc tttttgcatt ctgggcgctc tgagaggcca tagcgacggt tcctgcaaag
      961 ccatcgttca ctgcctggct taaacgtttg gctacggctg cctgatcttc cacgtctttg
     1021 gtgaaaatac ccttccgcag tacacgcggc tccagcgcct ccggtgagat cgatacctgg
     1081 ctacgggtgc cacggtttat cgattcgtag tagtgctgaa ctgtctgatg cgtggcacgg
     1141 cttccctcga taccgcgctc aagacccagt ttttttaccg attcagcgta ggtgctctga
     1201 tcgtcacgca tctttgaccg cccgccaata aactctttcg cggacagccg cccatcctgc
     1261 gtcaatggca ccacgaacgc agacaggtgc ggggtggcct cgtccctgtg aaccaccgcc
     1321 gccacaacgc gatctttccc gtatttgttc tccagccatt gctcagaacg ggcgaaaaac
     1381 tccgcctgct gccgcggtgt ggcctcgttc caccactcag ggcttgccgt cataacgtat
     1441 tcaacagcca gtaccgcatc tttacggcgt ttttccggca acagctcccg cagctttccc
     1501 atcgcctcat ctgccgactg agacacagag cagtagtttt ctggtgtccg ttcagcgtca
     1561 gcattcggcg tctcacgctc cctgtagcag tgctgcaaag cgctggccac gcttcccatt
     1621 cccgtcagct ttttgcagcg cattatcgcg taattcgcca tgcttacccc ctcgttctgg
     1681 cgtacatcca gccagtcggg ggatcgcatt gcgatgcctc cggcgggcca ctatcaggat
     1741 gatagtgtac tcactagact ttgctacgca aaatcgttcg ggcagcaagc tgccagaagg
     1801 tcaaaatcaa cggcagtctg gcaggtggaa ggcgtcgctc cgcgccaccc cccaccactg
     1861 ccaagcctgt tttatccggt gagtcgtcaa gggtaaatgg cactcgcttc gctcgcccct
     1921 gacgaaatca ggcaatgaat atcgtttttg aaagagcagg aaccgctcgc cgcaagaacg
     1981 gattagggag cggcatcgcc gcgagtctgt ccgttcgagg aagcggaatt tccactcccc
     2041 gc
//
