LOCUS       NZ_CP070051             1549 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP070051
VERSION     NZ_CP070051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(90..566)
                     /locus_tag="I6K20_RS24970"
                     /old_locus_tag="I6K20_24975"
     CDS             complement(90..566)
                     /locus_tag="I6K20_RS24970"
                     /old_locus_tag="I6K20_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          646..754
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          complement(950..1048)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1148..1408
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="8.62e-98"
ORIGIN
        1 gattttgagg cggcaaaaga gcaatgcagg atagacaatc atggtcagaa cgttaacacg
       61 tagcccctga ttgtccgcct gccgctagct caatttgaaa acatggccat gattcggcgc
      121 tgaatatcac cgtctttgtt gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta
      181 gtgttgtatc ccttccaaac ttcatgtgga tcgagggcat acgcacaaac cttaccagca
      241 acggggattc taactaatgc attttttgat tctaagtaat tgagccaccg aataattgtg
      301 cttctgctaa ctccaagttt ttcagctagg aattgctgat ccgcaacgac agcgccacac
      361 gtaggatcga tatgctccgc taaaaatgag tacagcttca gtgctgatat gccttggcta
      421 tccttcagca gctctcgaac acgttcccaa ccttttgggg atacctgtgt aaaccttgaa
      481 tttttctttg cttcttcgag atcctcggtg tttttctttg cctcatctga aagttgtttc
      541 agtcgttttt ctgaatcaat aaacatgatt ttttccctct gaaaatccca tgcactaacg
      601 ttggttcaaa tctaagccat catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca
      661 tccaggatgc gacaacgttg tgtcatcctg gatgcgacag gtaaataaaa aacacactaa
      721 aaaaacaacc acttataaat cggcgtaaaa gatctattct aggcaagggt gttgcgtgca
      781 acgcaacgcc ctttgggggt gtcggggcga agccctgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttactcc tatgcaagcc tcgccagccg tgatttttgc cagatttcgt
      901 ggttttcacg ttcaacagct ccatcgtgtt tttgccgtcc gttcaccgtt aaaacgcctc
      961 agaaatcggg acaggatgtg taatcgtata accgcgcatg cacgccgata cgattaccac
     1021 ttggtcaggg cttcaccccg acaccccgca tcagtgcgta agtgctcaat gtggcttccg
     1081 gttctcttcc gcttcctcgt cgattcgctc gctacgctcg gtcatcgact tgcggcgagc
     1141 gggatcacct ctcgcatgtt gtcggtgatc ccggtgctgt tcttttgggg atgagtcgcc
     1201 agatagcccc tcaaacgcgc tcaaaagctc tcagacgcac gaaacgggtt ttagatgccc
     1261 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aagcgcgccg
     1321 atacccctaa ccaggggctt tcaggtcgcc aggaaccgcg ccagccccct gcaaagccca
     1381 tttgcacgga acaggcagcc atgcggttgg taggtggtcg tagaccgtcg gtgttgcagc
     1441 gatgggggtc tggggttctc cccagacggt ttccggtagc gctcaaggcg ctgccggaac
     1501 cgtggcggct cggttttttc tggagaacgg ggagcaaaaa cacgcaaag
//
