LOCUS       NZ_CP070050             5163 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1290 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP070050
VERSION     NZ_CP070050.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357432
            Assembly: GCF_016904115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1253.22x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,063
            CDSs (total)                      :: 4,942
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 38 of 142
            Pseudo Genes (incomplete)         :: 104 of 142
            Pseudo Genes (internal stop)      :: 29 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            Pseudo Genes (short protein)      :: 4 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1290"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1290"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(4833..5163,1..68))
                     /locus_tag="I6K20_RS24945"
                     /old_locus_tag="I6K20_24950"
     CDS             complement(join(4833..5163,1..68))
                     /locus_tag="I6K20_RS24945"
                     /old_locus_tag="I6K20_24950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            245..1171
                     /locus_tag="I6K20_RS24950"
                     /old_locus_tag="I6K20_24955"
     CDS             245..1171
                     /locus_tag="I6K20_RS24950"
                     /old_locus_tag="I6K20_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_015231136.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1174..1707
                     /locus_tag="I6K20_RS24955"
                     /old_locus_tag="I6K20_24960"
     CDS             1174..1707
                     /locus_tag="I6K20_RS24955"
                     /old_locus_tag="I6K20_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1773..1982)
                     /locus_tag="I6K20_RS24960"
                     /old_locus_tag="I6K20_24965"
     CDS             complement(1773..1982)
                     /locus_tag="I6K20_RS24960"
                     /old_locus_tag="I6K20_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            3715..4629
                     /locus_tag="I6K20_RS24965"
                     /old_locus_tag="I6K20_24970"
     CDS             3715..4629
                     /locus_tag="I6K20_RS24965"
                     /old_locus_tag="I6K20_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          3716..4629
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.802"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          246..1168
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.675"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtctgttctc cgagcttcct gatgaatccg atccgtttct tcagccattc gatgttgctg
       61 atgcgcatgt tcgtgatgct ccctggtgtt tttttgcggt gtgttgtggt cgtgcagtat
      121 atcgcttgcg gtcggctggt gcagggggta tcttccgtgc taattcgtaa aattagaagg
      181 aagcgcactt atatgtttct ctccttcgtc gagaaacagt gcgctcctgc agagggctga
      241 aaccgtggca acctatcacc tcagcgttaa atttggtggc aaggggcagg ctgcaaacca
      301 cgccgattac atcgagcgca aagagaaata ccgcgaccgt caggatttgg aatattccgc
      361 acacggaaac atgccggaat gggccaggga taatccttcc catttctggc aggcggctga
      421 ccagtttgaa cgtgcgaacg gttcgaccta tagggaactt gaaattgcat taccccgtga
      481 actgacaccg gaacagcgcc ttgaactggt gcaggatttt gtacgtcagg aagctggcga
      541 acggcacgcc tggagttttg ccattcacaa tccgaaagcg agcattgacg gcggcgaaca
      601 gcctcatgcg catatcatga tgagtcagcg tgtgaatgat ggcattgaac gtacaccgga
      661 acagtatttc cggcgctata acgccaggta tcccgaccgg ggaggtgcga aaaaggattc
      721 cggaaacctg acccccactc agcaaaaaga gcagttgcgg gaattgcgaa aacgctggga
      781 ggtgaagcac aacgaacata tgagaaagca tggctttgaa aggggcttca ttgactgccg
      841 gacgctgaaa gaacagggca ttgaacgacg tcccgaagtg catctgggtt ttgaagctgc
      901 tggacggctt gatgatgccc agcgtcatga catcatgcaa aaacggtctg aaccggacta
      961 ttcccgccat cttgaacagg cgcgaacggt cgggcatgac atcagggaat ggctggacac
     1021 tccggttaag caacatgaac tggtcagaag gcttcaacat gatgttgcgc gggagcatgg
     1081 ctttgacatg agagacagag tgagcagaca ggaccgaact gaacctgaac gggttaaatc
     1141 accgtcacgt ggattttcta tgggattctg aacatggcca gcacagaaga aaaattactc
     1201 acgctcctgt tatcggtgga gcatctgcaa cataccgcaa tggagcaaca gaaagctgtt
     1261 gctgattctc tggctgtggc aagggaaaca cttcgcggat atcgggatat ttctgacggt
     1321 gtggcaaaac aggtcaggga tgaagtcaga aatgaggtgc agaagctgga cgtggcagga
     1381 ctgataggtg agcgcatcag ccaggatttt cagcggctgg aagagtcagt cgcaaacatg
     1441 aaggcaaacg cggatgccct gaacagggat attgcgacaa ttcacagcaa tatcgtgcag
     1501 gaatacaaca cccttaaagg gtatagctgg aaatggctgg gtggggtgtc agtggctttt
     1561 ctggttatgt tctgtgtgct cggatgggga atgaaaacaa tactcgacag gaactacaac
     1621 gccacagcgg cggtgtatca gaaagttgtg acactggaag ataaactggt ggagaaacct
     1681 gtcacgggga agagccggaa aaactgacgc gcttcgcttg tccaacgcct cacgacccgc
     1741 gaaaggattt cgcgaaccgt tcaccgtttt tttcaatatg attctgtctg ctttggtcga
     1801 taaagcagac gatagtattc cagccttcgt atatataacg gtacgtgctc atcagtaaga
     1861 gctggcggta ttgtgttatc agggggaagt tttttcagat actcacgaaa tgcctggctt
     1921 gatgccataa aatctacagt agcatcgata atatgtgggg tgttttcacc taattttgcc
     1981 atgatgataa tccttatatt atcacggttt gtgaatgata gggtgtcact attatttatg
     2041 cgttagtgaa ccagaaaaaa tcccccgttg gcataggggg atatgtaagg agaaataact
     2101 tcgcaggtgt tcttgtttgt ataacgtgaa aagactgcac gcaacttaat aagtctgccg
     2161 tacttacttt cagttttcaa cataaggttt ataacaatgt atggaatgga agtgagcttt
     2221 aaggtttcag ctctaaaaaa taaaatattt attagttggc tggttgttgt tcttgttctg
     2281 tatgtatata aacactatcg acatattatg accgggaaca ttccttaccc cctctgcgct
     2341 cgcagaggtt ttttttgtca ttaattaata atgaactgaa atgcagttcc gatacctcca
     2401 tttgcacatt atccctgaca tatggtaccg gaaccgcatt aattttatgt tataagagtt
     2461 tatttttaat gtccagatgg tttaatagta tttatatgta attagctgta tggatagtac
     2521 tggttttatt gtataacttg tcgttttttt attgtttttg tgtggtgtgc taatctttaa
     2581 gttataaaaa aaccccttgg aatacagggg caaaagtaag gaaaacaaat ggatgctcag
     2641 tttgtgtgac actttaaact aatggaacta tagagtggag gttgttacag tcgcttttat
     2701 aaaacagcaa ccagtttttt tcaatagtct gatgatggtt gtgacagttt tgtgatattg
     2761 ttcaaaatgt ttcttgttga atgtttgttt tttggtctat tggtgtctgg ttttattttt
     2821 tctgctatta catatcaaga aatgtgattg gtgtagcaag agtggaatgc gatgtggata
     2881 aactaattgg ttgtcgaatc taatttactt agaaaactac acttgttttt gtgtatgaat
     2941 ttagtgaatt tgtctgtctg tgtgttttcg tgcatttata aatccttcgt caggatctta
     3001 aatttagtgt tgaatatgta gggcctgtac ggtaattatt atttagcgta gctacttcat
     3061 cgttgttcga acacaaccaa caacctgtaa ttgttgtttc tcttggttcc ccacttttgt
     3121 ggggtttttt atctcttccg tgcatcgtaa agcgcggact ccttctggaa aggttgtttt
     3181 agggcatcac tgaggcattt tttgcgtttg tgaatattga gcatccgata actaaaatat
     3241 ttcccataga ctctgattct tgccaccgac cccaggtcgg tttttttttg tcctggcgag
     3301 atgtgaagtt tggaaacagg aaaccccgtc agagcgccac gcaccagggg agaaaacttg
     3361 tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcacatcg ctttcgtgca
     3421 tagttatgca gccctctaaa aacgattctg gcgcgttttt ctggtttggc ctggtgtttt
     3481 cttgtctttt tgcgtttttt gcgccagaaa gcgactgagg gcgttttaag gggtacgtac
     3541 aacgggagtt atggtaaatg gatcggtttt tcgggaagga tcgacaggat ttgccgttgg
     3601 gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt cgtgtctatc cgccaagact
     3661 ttcacgaaaa tacaggggcg atgtcggtcg ataacctgga aggaatcgta acacatgagc
     3721 gccgcgcttc aatacttcga agaaaattta ccccaccgtc cctatcacac ggatgatctc
     3781 gctttcggcc ttcgtatctc cggcaaaggg cgcgcgcttc ttgcacggta tatccagcag
     3841 aaccagcctc atgcacagtt ctggctggtt tttgacgttg accgcgaggg ggctgcgatt
     3901 gactggagcg accggaacgc tcccgcgccg aacatcaccg ttaaaaatcc tgtgaacgga
     3961 cacgctcacc tgctctacgc actcaacatc gccgtgagaa ccgcgcctga tgcctcggtt
     4021 aaggcgctga aatatgccgc agcggttgaa cgttcgctgt gtgagaaact ttgtgcggat
     4081 gtgaactaca gcggcctgat ttgcaaaaat ccgttccacc tggaatggct ggtgatggag
     4141 tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
     4201 gagcgtcgta gcatcgataa acattacggg atggggcgaa actgccatct gttcgaaatg
     4261 acgcgcaaat gggcttacag ggcgattcgt cagggctggc cagcgttctc acagtggctt
     4321 gaagcggtga tccagcgtgt cgaaatgtac aatgcatcgc ttcccgttcc gctttcactg
     4381 gctgaatgtc gggctattgg caagagcatt gcgaaataca cgcacaggaa cttcacgccg
     4441 gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc tgcacgcggt
     4501 cgcaagggcg gttctaagtc taagcgcagc acagtagcta catctgcacg cacgctgaaa
     4561 ccgtgggagg cattagggat cagtcgcgcc tggtattacc aactgaaaaa acgaggtctc
     4621 gtagagtaga ccaaataagc ctatatcaga taacagcgcc tttctggcgt ctttttgagc
     4681 agcaggtttt ttgccgtttt tccccgttca gccctgcaat ggcgcggctt tccgcgtgat
     4741 tgtggtggta gtgctcgccg cagtctcatg accgagcgta gcgagcgaat gagcgaggaa
     4801 gcaccaggga atcggaacac ggatggaggc acttacgcgt ctgggtttgg ggatgtctcc
     4861 agatcgtact taagttcctt aagtgcttgt aatatcctgt cttttttata gcatagctcg
     4921 cctgtttttg tgtctaccag tcctgcttcg atcagtagcc cagccgcgag aaagagccgg
     4981 tgattgcgtt cccgtcgttg tttttttccc tcgattcgtt tttggaccgc ctgttttcgt
     5041 tcgctctcct gtgcctgtaa gtcgtttttt tccgctgttg ccagtacgtg gagaagtttg
     5101 cgttcctgtt ctgtgagtcc ggcttcgttg tcgatcaggt caataatctg tctctgtcgt
     5161 gcg
//
