LOCUS       NZ_CP070043             1234 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1293 plasmid unnamed2.
ACCESSION   NZ_CP070043
VERSION     NZ_CP070043.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357435
            Assembly: GCF_016904055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1284.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:33:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,864
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 98 of 222
            Pseudo Genes (incomplete)         :: 132 of 222
            Pseudo Genes (internal stop)      :: 49 of 222
            Pseudo Genes (multiple problems)  :: 51 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1293"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1293"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..314)
                     /locus_tag="I6K23_RS24630"
                     /old_locus_tag="I6K23_24630"
                     /pseudo=""
     CDS             complement(<1..314)
                     /locus_tag="I6K23_RS24630"
                     /old_locus_tag="I6K23_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(317..>1234)
                     /locus_tag="I6K23_RS24635"
                     /old_locus_tag="I6K23_24635"
     CDS             complement(317..>1234)
                     /locus_tag="I6K23_RS24635"
                     /old_locus_tag="I6K23_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_204628227.1"
                     /translation="YHPSVKFGGTGQAANHADYIERKEKYRDRQALEYSAHGNMPEWAR
                     DNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWSFAI
                     HNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLTLTQ
                     QKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRLDDA
                     QRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFDMRD
                     RVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(321..1234)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.705"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgtgaattg tcgcaatatc cctgttcatg gcatccgcgt ttgccttcat gtttgcgact
       61 gactcttcca gccgctgaaa atcctggctg atgcgctcac ctatcagccc tgctacgtcc
      121 agattctgca cctcatttct gacttcatcc ctgacctgtt ttgccacacc gtcagaaata
      181 tcccgatatc cgcgaagtgt ttcccttgcc acagccagag aatcagcaac agctttctgt
      241 tgctccattg cggtatgttg caaatgctcc accgataaca ggagcgtgag taatttttct
      301 tctgtgctgg ccatgttcag aatcccatag aaaatccacg tgacggtgat ttaacccgtt
      361 caggttcagt tcggtcctgt ctgctcactc tgtctctcat gtcaaagcca tgctcccgcg
      421 caacatcatg ttgcagcctt ctgaccagtt catgttgctt aaccggagtg tccagccatt
      481 ccatgatgtc atgcccgacc gttcgcgcct gttcaagatg gcgggaatag tccggttcag
      541 accgtttttg catgatgtca tgacgctggg catcatcaag ccgtccggca gcttcaaaac
      601 ccagatgcac ttcgggacgt cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga
      661 agcccctttc aaagccatgc tttctcatat gttcgttgtg cttcacctcc cagcgttttc
      721 gcaattcccg caactgctct ttttgctgag tgagagtcag gcttccggaa tcctttttcg
      781 cacctccccg ctcgggatac ctggcgttat agcgccggaa atactgttcc ggtgtacgtt
      841 caatgccatc attcacacgc tgactcatca tgatatgcgc atgaggctgt tcgccgccgt
      901 caatgctcgc tttcggattg tgaatggcaa aactccaggc gtgccgttcg ccagcttcct
      961 gacgtacaaa atcctgcacc agttcaaggc gctgttccgg tgtcagttca cggggtaatg
     1021 caatttcaag ttccctatag gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc
     1081 agaaatggga aggattatcc ctggcccatt ccggcatgtt tccgtgtgcg gaatattcca
     1141 aagcctgacg gtcgcggtat ttctctttgc gctcgatgta atcggcatgg tttgcagcct
     1201 gtcccgtgcc accaaactta acgctggggt gata
//
