LOCUS       NZ_CP070040             1917 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1322 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP070040
VERSION     NZ_CP070040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357464
            Assembly: GCF_016904035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 919.04x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:50:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,346
            CDSs (total)                      :: 5,216
            Genes (coding)                    :: 5,088
            CDSs (with protein)               :: 5,088
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 57 of 128
            Pseudo Genes (incomplete)         :: 82 of 128
            Pseudo Genes (internal stop)      :: 22 of 128
            Pseudo Genes (multiple problems)  :: 27 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1322"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1322"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            493..729
                     /locus_tag="I6K52_RS26605"
                     /old_locus_tag="I6K52_26605"
     CDS             493..729
                     /locus_tag="I6K52_RS26605"
                     /old_locus_tag="I6K52_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(989..1291)
                     /locus_tag="I6K52_RS26610"
                     /old_locus_tag="I6K52_26610"
     CDS             complement(989..1291)
                     /locus_tag="I6K52_RS26610"
                     /old_locus_tag="I6K52_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          155..328
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(3..182)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.818"
                     /coverage="95.0"
                     /evalue="1.93e-67"
     Typing          complement(1396..1891)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.0"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 tttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc gcggccttat ctcttccctg
       61 ttaagctcct tcctggtatc tcccaggaaa tttccgcccc gcctgtaagc cggcaccgct
      121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatatcctg
      181 tactacatat cctgctgacg cgctggtgcc gccttttttc tcctgctaca tgaagcactt
      241 cactgatttc cacatccgtg ccaacatagt cagccagtat acactccgct agcgctacgt
      301 gactggttca gggctgcgcc ccgaaacccg ctaaaagcta atgacgcgcc tgcggcttgt
      361 ccaacgcctc acggcaccgg attaaaatcc gctcccgttc accgtttctg actgcctttc
      421 accgtcgctg gcgcttcatc gtttctactg tgtctggcag actgtgctga tttattctga
      481 ctggagaacg ttatggccag actgacaaac ttgacgcctg cagagaaaaa atttcttgat
      541 gatgctgttg ccgcagctga aagagcgtca ggtaaaaagc taaatcagcc taaccggcac
      601 atcgtcctga acagagcgcg tgcgcaaatt gagttgcagc gttacgccga ccgccagcgt
      661 gcattacgtg aggatgagcg gcagcagtcc gattttgcct ggtcacgtcc gcgcgcgccg
      721 cgccgctgat tttggctttt ggcggtttag tggtgctgac gctgcgaaat cacctgccat
      781 tcacctgcgt gcatagccat gcagtcgcgc agaaatcatc aggggcgttt ttccgggata
      841 ctggtgagga aaggtgctgg aaacggccct gatgcagcag gattgcgctg agggcgcctg
      901 cgggggtgcg ggcaagggat gttatgttta atcggtctga ccgtcaggta caaaaaaaag
      961 cccttagggg gctttttttt gctatgagtt agccatattt tcgtgcgaat ttttcatcgg
     1021 aatcgcatag ataaataata aactcaataa acgcgatgat cgcgggtatg aatgtccagc
     1081 aaaaaattag atacaggaac ccctgtccta tttttcctaa gtaaaattta tgtgcgccaa
     1141 atccaccaag gaagaatgct aatagcgcgg ctgcgattct acttttttca cctgaacctg
     1201 aagaggcgtt tgtggctccg cagtgcgggc atgactttgc tgtttcgtgt atttccttgc
     1261 cacatcctcg gcagtaaacc attgaactca ttttttatcc tcgagcttat tttaattggg
     1321 atcataatat ccgatgtttt ataacgatgt gtatcctatg tgctcggatt ggatagattt
     1381 ccattacggt gaaggatctt cttgagatcc tttctgtggc gtcgtaatct cttgctctgt
     1441 aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt ctcagagcag caactctttg
     1501 aaccgtggta actggcttac gaggagcgca gtaaccaaat ctgttctttc agtttagcct
     1561 taacaggcgc ataacttcaa gactaactcc tctaaatcag ttaccagtgg ctgctgccag
     1621 tggcgataag tcgtgtcttt ccgggttgga ctcaagatga tagttaccgg ataaggcgca
     1681 gcagtcggac tgaacggggg gttcgtgcat acagtccatc caggagcgaa ctgccttcgc
     1741 ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg
     1801 taaaccgaaa ggcaggaatg cgggggagca cgagggagcc accaggggga aacgcctggt
     1861 atctttaagc cgcatcgggt ttcgccacca ctgatttgag cgtcagattt catgatg
//
