LOCUS       NZ_CP070034             9009 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1337 plasmid unnamed5.
ACCESSION   NZ_CP070034
VERSION     NZ_CP070034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357479
            Assembly: GCF_016903995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 909.52x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 60 of 192
            Pseudo Genes (incomplete)         :: 133 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            Pseudo Genes (short protein)      :: 4 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1337"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1337"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            1766..1975
                     /locus_tag="I6K67_RS23660"
                     /old_locus_tag="I6K67_23660"
     CDS             1766..1975
                     /locus_tag="I6K67_RS23660"
                     /old_locus_tag="I6K67_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2041..2574)
                     /locus_tag="I6K67_RS23665"
                     /old_locus_tag="I6K67_23665"
     CDS             complement(2041..2574)
                     /locus_tag="I6K67_RS23665"
                     /old_locus_tag="I6K67_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2577..3503)
                     /locus_tag="I6K67_RS23670"
                     /old_locus_tag="I6K67_23670"
     CDS             complement(2577..3503)
                     /locus_tag="I6K67_RS23670"
                     /old_locus_tag="I6K67_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_151309484.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSKQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3680..4078
                     /locus_tag="I6K67_RS23675"
                     /old_locus_tag="I6K67_23675"
     CDS             3680..4078
                     /locus_tag="I6K67_RS23675"
                     /old_locus_tag="I6K67_23675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4282..5196)
                     /locus_tag="I6K67_RS23680"
                     /old_locus_tag="I6K67_23680"
     CDS             complement(4282..5196)
                     /locus_tag="I6K67_RS23680"
                     /old_locus_tag="I6K67_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(5643..7862)
                     /gene="mobQ"
                     /locus_tag="I6K67_RS23685"
                     /old_locus_tag="I6K67_23685"
     CDS             complement(5643..7862)
                     /gene="mobQ"
                     /locus_tag="I6K67_RS23685"
                     /old_locus_tag="I6K67_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063106751.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPVRRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
                     /name="mobQ"
     gene            8041..8316
                     /locus_tag="I6K67_RS23690"
                     /old_locus_tag="I6K67_23690"
     CDS             8041..8316
                     /locus_tag="I6K67_RS23690"
                     /old_locus_tag="I6K67_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052995416.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNG"
                     /name="hypothetical protein"
     gene            complement(8522..>9009)
                     /locus_tag="I6K67_RS23695"
                     /old_locus_tag="I6K67_23695"
     CDS             complement(8522..>9009)
                     /locus_tag="I6K67_RS23695"
                     /old_locus_tag="I6K67_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_199391272.1"
                     /translation="EWREEAYTLDELADYLDLSASERRSVDKHYGLGRNCHLFEITRKW
                     AYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSLAECRAIGKSIAKYTHRKFSPEGF
                     SAVQAARGRKGGLAKGEAYEDKRFMALCMLENGYSQKAIAAMLDVHRNTIRNWAMHK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          7505..7863
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7864..8034
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="97.0"
                     /evalue="1.23e-74"
     Typing          complement(4283..5196)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8523..9009)
                     /name="rep_cluster_2361"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2581..3503)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5647..7862)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.099"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
       61 cgacatcgcc cctgtatttt cgtgaaagtc ttggcggata gacacgaatt tcaggggcgt
      121 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagcct tcccgcaaat
      181 ccgatccatt taccataact cccgttgtac gtaccctgta aaacgccctc aatcgctttc
      241 tggcgcaaaa aacgcaaaaa gacaagaaaa caccaggcca aaccagaaaa acgcgtcaga
      301 aacgttttca gggcgttgca taactatgca cgaaagcgat gtgcgatcgg tttcgtgcgt
      361 tctgaacacc gaacggctca aaacgacaag ttttctcccc tggtgcgtgg cgctctgacg
      421 gggtttcctg tttccaaact tcacatctcg ccaggacaaa aaaaaaccga cctggggtcg
      481 gtggcaagaa tcagagtcta tgggaaatat tttagttatc ggatgctcaa tattcacaaa
      541 cgcaaaaaat gcctcagtga tgccctaaaa caacctttcc agaaggagtc cgcgctttac
      601 gatgcacgga agagataaaa aaccccacaa aagtggggaa ccaagagaaa caacaattac
      661 aggttgttgg ttgtgttcga acaacgatga agtagctacg ctaaataata attaccgtac
      721 aggccctaca tattcaacac taaatttaag atcctgacga aggatttata aatgcacgaa
      781 aacacataga cagacaaatt cactaaattc atacacaaaa acaagtgtag ttttctaagt
      841 aaattagatt cgacaaccaa ttagtttatc cacatcgcat tccactcttg ctacaccaat
      901 cacatttctt gatatgtaat agcagaaaaa ataaaaccag acaccaatag accaaaaaac
      961 aaacattcaa caagaaacat tttgaacaat atcacaaaac tgtcacaacc atcatcagac
     1021 tattgaaaaa aactggttgc tgttttataa aagcgactgt aacaacctcc actctatagt
     1081 tccattagtt taaagtgtca cacaaactga gcatccattt gttttcctta cttttgcccc
     1141 tgtattccaa ggggtttttt tataacttaa agattagcac accacacaaa aacaataaaa
     1201 aaaacgacaa gttatacaat aaaaccagta ctatccatac agctaattac atataaatac
     1261 tattaaacca tctggacatt aaaaataaac tcttataaca taaaattaat gcggttccgg
     1321 taccatatgt cagggataat gtgcaaatgg aggtatcgga actgcatttc agttcattat
     1381 taattaatga caaaaaaaac ctctgcgagc gcagaggggg taaggaatgt tcccggtcat
     1441 aatatgtcga tagtgtttat atacatacag aacaagaaca acaaccagcc aactaataaa
     1501 tattttattt tttagagctg aaaccttaaa gctcacttcc attccataca ttgttataaa
     1561 ccttatgttg aaaactgaaa gtaagtacgg cagacttatt aagttgcgtg cagtcttttc
     1621 acgttataca aacaagaaca cctgcgaagt tatttctcct tacatatccc cctatgccaa
     1681 cgggggattt tttctggttc actaacgcat aaataatagt gacaccctat cattcacaaa
     1741 ccgtgataat ataaggatta tcatcatggc aaaattaggt gaaaacaccc cacatattat
     1801 cgatgctact gtagatttta tggcatcaag ccaggcattt cgtgagtatc tgaaaaaact
     1861 tccccctgat aacacaatac cgccagctct tactgatgag cacgtaccgt tatatatacg
     1921 aaggctggaa tactatcgtc tgctttatcg accaaagcag acagaatcat attgaaaaaa
     1981 acggtgaacg gttcgcgaaa tcctttcgcg ggtcgtgagg cgttggacaa gcgaagcgcg
     2041 tcagtttttc cggctcttcc ccgtgacagg tttctccacc agtttatctt ccagtgtcac
     2101 aactttctga tacaccgccg ctgtggcgtt gtagttcctg tcgagtattg ttttcattcc
     2161 ccatccgagc acacagaaca taaccagaaa agccactgac accccaccca gccatttcca
     2221 gctataccct ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc
     2281 cctgttcagg gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa
     2341 atcctggctg atgcgctcac ctatcagtcc tgccacgtcc agcttctgca cctcatttct
     2401 gacttcatcc ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagagt
     2461 ttcccttgcc acagccagag aatcagcaac agctttctgt tgctccattg cggtatgttg
     2521 cagatgctcc accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag
     2581 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggtgcagt tcggttctgt
     2641 ttgctcactc tgtctctcat gtcaaagcca tgctcccgcg caacatcatg ttgcagcctt
     2701 ctgaccagtt catgttgctt aaccggagtg tccagccatt ccctgatgtc atgcccgacc
     2761 gttcgcgcct gttcaagatg gcgggaatag tccggttcag accgtttttg catgatgtca
     2821 tgacgctggg catcatcaag ccgtccggca gcttcaaaac ccagatgcac ttcgggacgt
     2881 cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc
     2941 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
     3001 ttttgctgag tgagagtcag gcttccggaa tcctttttcg cacctccccg ctcgggatac
     3061 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
     3121 tgactcatca tgatatgcgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg
     3181 tgaatggcaa aactccaggc gtgccgttcg ccagcttcct gacgtacaaa atcctgcacc
     3241 agttcaaggc gctgttccgg tgtcagttca cggggtaatg caatttcaag ttccctatag
     3301 gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc agaaatggga aggattatcc
     3361 ctggcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcctgacg gtcgcggtat
     3421 ttctctttgc gctcgatgta atcggcgtgg tttgcagcct gccccttgcc accaaattta
     3481 acgctgaggt gataggttgc cacggtttca gccctctgca ggagcgcact gtttctcgac
     3541 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     3601 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     3661 accagggagc atcacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     3721 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgatcga
     3781 caacgaagcc ggactcacag aacaggaacg caaacttctc cacgtactgg caacagcgga
     3841 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     3901 gggaaaaaaa caacgacggg aacgcaatca ccggctcttt ctcgcggctg ggctactgat
     3961 cgaagcagga ctggtagaca caaaaacagg cgagctatgc tataaaaaag acaggatatt
     4021 acaggcactt aaggaactta agtacgatct ggagacatcc ccaaacccag acgcgtaagt
     4081 gcctccatcc gtgttccgat tccctggtgc ttcctcgctc attcgctcgc tacgctcggt
     4141 catgagactg cggcgagcac taccaccaca atcacgcgga aagccgcgcc attgcagggc
     4201 tgaacgggga aaaacggcaa aaaacctgct gctcaaaaag acgccagaaa ggcgctgtta
     4261 tctgatatag gcttatttgg tctactctac gagacctcgt tttttcagtt ggtaatacca
     4321 ggcgcgactg atccctaatg cctcccacgg tttcagcgtg cgtgcagatg tagctactgt
     4381 gctgcgctta gacttagaac cgcccttgcg accgcgtgca gcctgaattt ctggcgtgtg
     4441 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
     4501 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
     4561 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
     4621 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
     4681 cccgtaatgt ttatcgatgc tacgacgctc tgaggcgctc aaatcgagat aatcagccag
     4741 ttcatcgagg gtataggctt cctcgcgcca ctccatcacc agccattcca ggtggaacgg
     4801 atttttgcaa atcaggccgc tgtagttcac atccgcacaa agtttctcac acagcgaacg
     4861 ttcaaccgct gcggcatatt tcagcgcctt aaccgaggca tcaggcgcgg ttctcacggc
     4921 gatgttgagt gcgtagagca ggtgagcgtg tccgttcaca ggatttttaa cggtgatgtt
     4981 cggcgcggga gcgttccggt cgctccagtc aatcgcagcc ccctcgcggt caacgtcaaa
     5041 aaccagccag aactgtgcat gaggctggtt ctgctggata taccgtgcaa gaagcgcgcg
     5101 ccctttgccg gagatacgaa ggccgaaagc gagatcatcc gtgtgatagg gacggtgggg
     5161 taaattttct tcgaagtatt gaagcgcggc gctcatgtgt tacgattcct tccaggttat
     5221 cgaccgacat cgcccctgta tcttcgtgaa agtcttggcg gatctacacg aatttcaggg
     5281 gcgtttgttt ttcagtcgct tacactacac ccaacggcaa atcctgtcga tccttcccga
     5341 aaaaccgatc catttaccat aactcctgtt gtacgtaccc cttaaaacgc tctcagacgc
     5401 gttctgacgc aaaaaacaca aaaagacaag aaaacaccag gccaaaccag aaaaacgcgt
     5461 cagaatcgtt ttcaggggtc tgcataacta ttcacgaaag cgatgcgcga tcggtttcga
     5521 ccgttcagaa gcgcgaacgg gtcaaaacgg caaactttct catccagaaa acgcctcacg
     5581 ggacgcggaa ttttttcgtg tcccgtgaga cgttggcggg gttcggggtg cagccccgga
     5641 tatcacatac tcatgccccg gtaatgtccg gggcgtcgtt gttgttgcct gtgctgttcg
     5701 tcccgcttcc attcatgcca ggtctgatta aatccgagtt gacggaagag ataatccggc
     5761 atctcttccg gtttgttgat ggcagcaaaa agcgtgtcct gacagccctt aagcctgaac
     5821 tgcatcagct tcggttccgg tgggtacgga gtatcagcat cacggtttat ggcctcaccg
     5881 tagcctgcca ggtgtatccc gttctctgtg atgatgctta ccatcagatg actgtaatcc
     5941 ggcgacggaa gcgcaagaac gggacgcagc tcgtcctcca ggcgcacaaa accgacaagc
     6001 atttcctccg gccctttgca caacagcaga ttcagcgtcc tgctgtttcg ctctgtcgaa
     6061 aatacaacgg gcatgagttg ctccggtgcc gcataagcgg tgacgtccac ccggtgcgct
     6121 gacggcagcg gagaggctgc gggcctgcgg tcgtcgctga aataaaaatt atccggccag
     6181 tgatgcggca gcgccggaaa accgtaagag ggccacccct ggcgcatctg ttccagtaca
     6241 tgccggaccg tcgcaccgga atacattctc acacgctcag gccgcgccat cgtctcatga
     6301 gattctggca ttccctcacg tatatccgag accatcacag ccccgcctgg cgacaggtaa
     6361 acattcaccg ggtcatgaac actctggggg taatgcgcca gctcatcacc ccataccgtg
     6421 aattctccgg ctctgttact cagcatcatg tagcgggtta tcctgcccgt tcccggctct
     6481 ctggcacgcc ccagctccgt aataaccccg gctaatgccg tttctcggct caggcgatgg
     6541 ttatcatggt gtttcagccg tgcgtcatat tcctgttcct tccggcgcgc ttccgcttcg
     6601 gcttcctccc tgcgccgctc cgcttcccgt gctgacagtt tttctttcag ctctgccatt
     6661 cccggcgaaa tccacgtatt ccactgcgtt tcaaaggcct gacgacggac gggaatgtcc
     6721 cgttctttca cggccctgta ggtgcggaga agagattcaa tctgtttctc gagtttccag
     6781 aagcggagtt gtcttttgcg gtattccggt tcagcccatg tcatcagggg aagctctgac
     6841 aggatggacg ctaccggacg ggcttttctg agtccggtca tctctttttc gagaatatcc
     6901 cgctctttca ccagcgcatt cccctcacgt ttgatgtcgt ttgccttccc gttcatcgcc
     6961 acgaactccg gtgtcatctc ccatgcgttc ccgcattcct gcggcagtgt ggcaatgaat
     7021 tcccgcaggg tatccgctga ggcattcgcg gcgggaatgg tcagattaag ggcttccgct
     7081 gcacgacgtg cggcatcgct gagttcccgt ttccgcacgt tctcctgtac ctggatgact
     7141 gccagccttg tgcgggaaac ctgtatttcc aggttaaggc tgttaatcag gcggttctgc
     7201 tcaccgcgtt ctgtttccat gccccgtttt tccatcgcac aggcagcctt tccgaggtga
     7261 aggcccggcg ctttctccag tccctgacgc tcgtatgaac gatggtctat ctcttcctgg
     7321 tagccggcgt tcgcaagcgc tctgttcgca tggtcagccc atgatgcgcg ccacgtctcg
     7381 gcgagtgccc ggtcgttcca gtccctgacc tttcctgcga atcccgtttc cccgacagct
     7441 ctcgtggtca gcatgatgtg tgcatggggg ttggtccggt ccatatgatg gaacgccaca
     7501 tcggcaatca tgccccgact gacaaagttt tcccggacaa aagcgagaac ggcctcccgt
     7561 gcggcctcgc gggaaatctc acggggaatg gcgagttcaa tctcccttgc cagctgggag
     7621 ttccggcgct gttcggcttt ctcgaccgca ttccagagaa cggaacggtc accacaccag
     7681 gacggtgcat tcgcaggggt gagaatgacc gcatcatcaa cgccgcgttt acgggtgtaa
     7741 tcgtggatta atccggtacg gtcatcgggt atgacagagc cggaacggta ggcagcagaa
     7801 gcaacggcac tgtagccgtt ttttcgcgaa atgattttca tgctgagatg ataaatcgcc
     7861 atttctgccg taatgccttt ttgccgtttt ctttcagccc cgcagggcgc acacaatcga
     7921 aacgaagtga agattgtata agtgcgcact tcgtgatatg ccgtgcatta tgccagctca
     7981 gtttttaaac taccagtgac gagctaaaat gcgatttttc accgcgcaag gagtcacgaa
     8041 atgagcagaa cactcgaaca gaagattgct gaagctgagg ccagattgca gcgcctgaag
     8101 gcgaagagcc ggagcctgga cactgcgcaa aaagtcattg tgggagcagc catgctggcg
     8161 agggtacgga ggccggaaga ggcgcaactg cgtgcgttcc tgctccagtt cctgcgaaag
     8221 gaagtcacaa gacaggccga tgtgaaccgg ctacaaccac tgattaacga gctggagaag
     8281 ctaccaagac cacctgcgaa accgcagaac ggataagccc cggcgcgtaa gtgccacatg
     8341 caccaccgga cgcctttgcg cttcctcgca cattcgctcg ctacgctcgg tcatgaggct
     8401 gcggcgagcg ctaccaccgc aatcacgagg aaaccagcgc cattgctggg cggaaccgga
     8461 aaaaccgcag gaacgccaac agctcaaaaa agcgccgaaa aggcgctgtt atctgataag
     8521 gctatttgtg cattgcccaa ttacggatcg tatttcgatg tacatccagc atcgccgcaa
     8581 tagctttctg agaatatcca ttctccagca tacatagcgc catgaaacgc ttgtcctcgt
     8641 aggcttcgcc tttagcaagg ccacctttcc ggcctcgagc ggcctgtaca gcggaaaagc
     8701 cttccggtga gaattttctg tgcgtgtatt tcgcaatgct cttgccaata gcccggcatt
     8761 cagccagtga aagaggaacg ggaagcgatg cgttgtacat ttcgacacgc tggatcacgg
     8821 catcaagcca ctgtgagaat gctggccatc cctgacgaat cgctctgtaa gcccatttac
     8881 gcgttatttc gaacaggtgg cagtttcgcc ccagcccgta atgtttatcg acgctacgac
     8941 gctctgaggc gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc
     9001 gccactcca
//
