LOCUS       NZ_CP070033             9822 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1337 plasmid unnamed4.
ACCESSION   NZ_CP070033
VERSION     NZ_CP070033.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357479
            Assembly: GCF_016903995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 909.52x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 60 of 192
            Pseudo Genes (incomplete)         :: 133 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            Pseudo Genes (short protein)      :: 4 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1337"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1337"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..417
                     /locus_tag="I6K67_RS23610"
                     /old_locus_tag="I6K67_23610"
     CDS             <1..417
                     /locus_tag="I6K67_RS23610"
                     /old_locus_tag="I6K67_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="WP_285804148.1"
                     /translation="VFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERA
                     KRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDPG
                     LIGGYGKNQMVCRAPLKSLNNLEAAAVYKKITLI"
                     /name="glycosyltransferase family 9 protein"
     gene            complement(465..1718)
                     /locus_tag="I6K67_RS23615"
                     /old_locus_tag="I6K67_23615"
     CDS             complement(465..1718)
                     /locus_tag="I6K67_RS23615"
                     /old_locus_tag="I6K67_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="WP_022646273.1"
                     /translation="MEVKIEQFRNNSCNFIFLLYLIGLLFVPIDLNITTKIINATGAFS
                     LFLLIVNKKHNYNKNVIILILGLLLLGAMNLIWYEFYKTEDVVYRNAYRGYLESGKLLV
                     FSSLTFLLLSKNKFRQNEKLHFISAILVQIIMLCRAYYQGIYLHADRIPLSAMNGNIGQ
                     MGAATIAAYVITFTTLYSSIVFIKMQSKYKWFLFYANFALSFAAVMMTGTRAAIFTFPL
                     MIMVILFLQHRDQKVFLFKGLSGVFILLLACGLIFNKEIDRRINSLKADVISYATKNNS
                     QSSVGARFAMVNAGIKGSPDGFNWQSLEQRAEKIKALSAENNIYSGALLFLDVHMHNEI
                     VESLSTKGKIGLLVLIMFYVAMIYYCIREKKYILLVFPASIMLFGISDVITHAKPIPAS
                     WIVCLFLSISFLSKKEPQ"
                     /name="O-antigen ligase family protein"
     gene            complement(1753..2526)
                     /locus_tag="I6K67_RS23620"
                     /old_locus_tag="I6K67_23620"
     CDS             complement(1753..2526)
                     /locus_tag="I6K67_RS23620"
                     /old_locus_tag="I6K67_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="WP_001626263.1"
                     /translation="MNLPIYIVSLKRDIERRNKINDVFHRLNINFDFFDAIDAKDPQNK
                     EIIDKMRLSGVGAEMTDGEIACTLSHQLIYQDMIDKNIEWAVILEDDVIVNEKFKKFLQ
                     YFNLSEKDKLKHNNLYLLGGQKGLHDYPVLGQSLFSKVKVSTCTFRRVNFNKNKIRRTC
                     SYLMNKDMAQKLLKLTKDYGTYRADSWKLMHQHNIIKEFYLDEIILHPILNEFNSHLES
                     ERLLTSEKKQPRTRLQKRMKFIRSWIKVAFFSLLK"
                     /name="glycosyltransferase family 25 protein"
     gene            complement(2558..3586)
                     /locus_tag="I6K67_RS23625"
                     /old_locus_tag="I6K67_23625"
     CDS             complement(2558..3586)
                     /locus_tag="I6K67_RS23625"
                     /old_locus_tag="I6K67_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="WP_022646274.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQSV
                     VMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQWSY
                     ATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQEKP
                     AKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDILNIL
                     FFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWTNYPCQ
                     KYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGKIASSLYYFMLYYSKKIARKHF
                     SK"
                     /name="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
     gene            complement(3612..4304)
                     /gene="rfaY"
                     /locus_tag="I6K67_RS23630"
                     /old_locus_tag="I6K67_23630"
     CDS             complement(3612..4304)
                     /gene="rfaY"
                     /locus_tag="I6K67_RS23630"
                     /old_locus_tag="I6K67_23630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="WP_000633089.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDDT
                     KVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFY
                     FLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHR
                     GNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIVNEIKDYGYYSVIYRTKLRKF
                     IKKLKGKA"
                     /name="rfaY"
     gene            complement(4314..5309)
                     /locus_tag="I6K67_RS23635"
                     /old_locus_tag="I6K67_23635"
     CDS             complement(4314..5309)
                     /locus_tag="I6K67_RS23635"
                     /old_locus_tag="I6K67_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_001001728.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSVL
                     INNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYAT
                     YFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKR
                     LNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNM
                     NNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYF
                     NIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
                     /name="glycosyltransferase"
     gene            complement(5326..6342)
                     /gene="waaO"
                     /locus_tag="I6K67_RS23640"
                     /old_locus_tag="I6K67_23640"
                     /gene_synonym="rfaI"
     CDS             complement(5326..6342)
                     /gene="waaO"
                     /locus_tag="I6K67_RS23640"
                     /old_locus_tag="I6K67_23640"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /GO_function="GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 3-alpha-galactosyltransferase"
                     /protein_id="WP_022646275.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGCG
                     VSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALPTT
                     KNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTERDAN
                     WWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTYMDQD
                     ILNLILLGKVKLIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGYPS
                     AQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYLKIIK"
                     /name="waaO"
     gene            complement(6358..7155)
                     /gene="rfaP"
                     /locus_tag="I6K67_RS23645"
                     /old_locus_tag="I6K67_23645"
     CDS             complement(6358..7155)
                     /gene="rfaP"
                     /locus_tag="I6K67_RS23645"
                     /old_locus_tag="I6K67_23645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="WP_021516572.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGK
                     SYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGINP
                     LTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDC
                     YICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRD
                     IWRFMKVYFVAPLKDILKQEQGLLSQAEEKATKIRERTIRKSL"
                     /name="rfaP"
     gene            complement(7148..8272)
                     /locus_tag="I6K67_RS23650"
                     /old_locus_tag="I6K67_23650"
     CDS             complement(7148..8272)
                     /locus_tag="I6K67_RS23650"
                     /old_locus_tag="I6K67_23650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_022646276.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGEC
                     PDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREYPVDRVVGFNKMPGLDVYYAADVCYA
                     EKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAERFH
                     ILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLP
                     DSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQE
                     AAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKALTQSSL
                     RQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(8269..9327)
                     /gene="rfaQ"
                     /locus_tag="I6K67_RS23655"
                     /old_locus_tag="I6K67_23655"
     CDS             complement(8269..9327)
                     /gene="rfaQ"
                     /locus_tag="I6K67_RS23655"
                     /old_locus_tag="I6K67_23655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase RfaQ"
                     /protein_id="WP_000360395.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQD
                     TIPILSENPEINALYGIKNKKAKASEKIANFFHLIKILRANKYDLIVNLTDQWMVAILV
                     RLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQTTMS
                     YPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVLTS
                     GPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAAAVN
                     TPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVIAAVD
                     KLLPSSTTGTSL"
                     /name="rfaQ"
     gene            9740..>9822
                     /locus_tag="I6K67_RS24400"
     CDS             9740..>9822
                     /locus_tag="I6K67_RS24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_237901471.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKA"
                     /name="hypothetical protein"
ORIGIN
        1 gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa
       61 ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat
      121 gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag
      181 atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat
      241 acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca
      301 accgatcctg gattaattgg tgggtatggg aagaatcaga tggtttgtag ggctccgcta
      361 aagagtttaa ataatttaga agcagcggct gtttataaaa aaatcacatt gatttaataa
      421 atagtaagtg ggcattatac ccacttactt agtcaacgct tttattattg tggttctttt
      481 ttactcaaaa acgatataga caggaagagg cacacaatcc acgatgccgg tattggttta
      541 gcatgtgtaa tgacatcact aatgccaaat aacataatag aagcagggaa aaccaagagt
      601 atatattttt tctcccggat acagtaatag atcattgcaa catagaacat aataagaaca
      661 agaagaccaa tttttccttt cgtggaaaga gattccacaa tttcgttatg catatgtaca
      721 tcaaggaata ataaagctcc actataaata ttattttctg cacttaatgc ttttatcttt
      781 tcagcacgct gctcaagaga ttgccaatta aaaccatcag gacttccttt aattcctgca
      841 ttaaccattg caaaacgtgc tccgaccgag gattgactgt tattttttgt agcatatgaa
      901 ataacatcag cttttaatga attaattcgc ctatcaattt ccttattaaa tatcaaacca
      961 caagcaagta gcaatataaa aacaccactc aatcctttaa ataaaaacac tttttgatca
     1021 cgatgctgaa gaaataatat gaccataatc ataagtggga atgtgaaaat tgccgcacgc
     1081 gttcctgtca tcattactgc tgcaaagctt aaagcaaagt tagcataaaa tagaaaccat
     1141 ttatatttcg attgcatttt gatgaataca attgaagaat ataaagtagt gaatgtaatc
     1201 acataagccg caatagtcgc tgcccccatc tgaccaatat tcccattcat ggcggagagg
     1261 ggaatgcggt ctgcatgcaa atatatccct tgataatatg ctctacatag catgattatt
     1321 tgaactaaga tagctgaaat aaaatgtagt ttttcatttt gcctgaattt atttttactt
     1381 agtaataaaa atgtcaacga gctaaatact aatagttttc cagactcaag gtagcctcga
     1441 taagcatttc tatatactac atcttctgtt ttataaaatt cataccatat caggttcatt
     1501 gcacccaaaa gaagtaaacc aagaattaat ataatgacat ttttattata attatgcttt
     1561 ttatttacaa ttaataagaa aagagaaaat gcacctgtcg cattaattat tttagtggtg
     1621 atattaagat caattggcac aaacaacaaa ccgattaaat acaataaaaa aataaaatta
     1681 cagctattat ttcgaaattg ctcaattttt acttccacgt taatatctca caacaaattg
     1741 aaattaacaa tctcatttca aaagagaaaa aaaagccacc tttatccagg agcgtataaa
     1801 cttcattctt ttttgaagcc ttgtcctggg ttgttttttt tccgatgtta atagcctttc
     1861 actttcaaga tgactattaa actcatttaa aataggatgt aatattattt cgtctaaata
     1921 aaactctttt ataatattat gttgatgcat taacttccag ctatcagctc gataggtgcc
     1981 ataatccttt gtcaatttga gcaacttttg tgccatatct ttattcatca aatatgaaca
     2041 tgtacgccga attttatttt tattgaaatt aacacgccga aaagtacaag tacttacctt
     2101 aactttacta aaaaggcttt gccccaatac cggataatca tgtaatcctt tctgtcctcc
     2161 aagcaaatac aaattattat gttttaactt gtctttttca gaaagattaa aatattgcag
     2221 aaatttttta aatttctcat tgacgataac atcatcctcg agaatgactg cccattctat
     2281 atttttatct atcatatcct gatatataag ttgatgactt aatgtacaag caatttctcc
     2341 atcagtcatt tctgcgccga caccacttaa tctcatttta tcaataattt ctttattttg
     2401 agggtcttta gcatctatgg catcaaaaaa atcaaaatta atatttaatc gatgaaaaac
     2461 atcatttatt ttatttcttc gctcaatatc tcttttaagt gagacaatat aaatgggcaa
     2521 gttcattaaa aaatctctgg atgattaatc ttaataatta tttggaaaaa tgcttacgcg
     2581 ctattttctt actataatag agcatgaaat agtaaagtga agatgcaatt tttccatttc
     2641 ttttcaaatg tctggatttc acttgatact gcttttcatt cgtggctgga ataaacgcca
     2701 catcattcca gcaagaagcc tgataagcct cattaaaata cttctggcag ggataattcg
     2761 tccagtcgtt ccagggcttc gttacgccaa cataatgaat cagcttagta tcatcggtaa
     2821 tggtcttttt ataatcttca tcgcttttat tttttaactc ataatcaatg ccataaaagc
     2881 aatcataatc gccgctaata aaaatacaat gaccaaagaa cagaatattt aaaatatcct
     2941 gatcaaggca tttatacttc gtgtgctgcg ggtcacttgc cagcatagca atggctttat
     3001 tcataaaatc atttttcgcc catgcttcaa gctgcagata tactacaccg gcgttgaaat
     3061 aattccctgg aagtccctct atagccagac gcttcgctgg tttttcctgc ataaaaggca
     3121 catctttaat aacagctgcg aacttatcgc catcaaaatt aatatcaagc aatccagcta
     3181 atgagccttt acagatgaca tcagcatcga tatataacag attttcaata gtttcactca
     3241 gatactggaa cgcaactaaa cggaagaatg ttgcataaga ccactgtttc aggctgggga
     3301 agatatcaat aaaggcactg gagactttat aaattcttac ttcaacgtta gcatttttgc
     3361 tggtaaaagc attaacacgt tgtagataat cttcatcaat gtagtcagta aagagatgaa
     3421 acttaaccgc aaggtccggg ttatgcataa caactgattg catagatact gccgcaccaa
     3481 ataaaaagtt gcggtcaata ccgtaagcga tatttaacac acgatcggtg ttagcaattt
     3541 ccccagagac agcgacttca gtaatactct cagctaataa atccatgttg gttccgtttt
     3601 gactgtgtgg tttacgcttt gccttttaat tttttaataa atttgcgtag tttggttctg
     3661 tagattactg aatagtagcc ataatctttt atttcgttga caatacccag gtgacgttcc
     3721 atggccagac gatcacgagc tttacgctct gccgtacagc ttttaccgga taggtcaatt
     3781 atcctgacac catctttact aacgataaag ttaccccggt gtgggtcgcc cgatagcata
     3841 tttaaggcat gcaatttttc cattgaagcc ttaatttctg ctttaacatt ttctggaata
     3901 atcggcatat cattgagctc tacgccctcg acatactcga taagcatgat aaaaacactg
     3961 gcatagttga aaattttacg ctcagccaga aaataaaaat cattaggaaa agtaagacct
     4021 gcacttctga cacgatcggt ttcaacgatc aaattctgat agtagtcacc ttttacaaaa
     4081 gattttaaaa aacgttcatt ccttttcgtt ttcggagcaa agactttaaa aacatagcga
     4141 ccatatttcg tatcaatcaa cgatactttg gtatcatcaa tattacgtaa tacttttact
     4201 gttttaaaat tataggccag tatttcatca aagatttctt tatatttatt atcattatct
     4261 ttgtaataaa atgagaagcc gcgataacgt atacttgtaa tcataagagt aatttatttc
     4321 ttaagcttgt acttaattaa tgaagttatg ccttttatat actcaccatg ggcaaacaga
     4381 tgcttatatt gtttctgcat ttctgcaaca gtccgcgcct ctttaagagg atatttcttc
     4441 cagggagatt gttcacgcgc gatattaaag tatgatgcag acggatatcc agcccagcta
     4501 tgccatggtt tagttatccc tgtatagtga atcaatactg ttttatctgt aatggtttgc
     4561 tgatactttc gatgactacg atcatgaagt tcgtttttca gggtataaat agtatcaaaa
     4621 tccttcgcga ggtagatatt attcatatta aatgcgatat tcaacgcatc ctgatctaaa
     4681 tatttaagag agccatattt agtttcccct cgtaaaagcg tgagtaaata cggtgtcaaa
     4741 tttgcttcat gccatttttt aagattgaca tagataacgc ctgcattgaa ataacgtcca
     4801 ttcatttcgg gaatgttaag acgttttgca catgccgcct gagtactatc attgtcagga
     4861 ataaccgcag caaactcatc tttaaatata atttttgtga gaggtttcag acttccttta
     4921 caaacgacat cggcatccag atacagcagt gtggaaatac tttcactcag atattcaaag
     4981 gacaataccc tgaagtatgt cgcgtacgac caaaactgtg acgtcggcag atcagcaaag
     5041 tatttggggt caattaaata tacaataatt gaggtattaa attgttttgc tgtttcatta
     5101 aatgacttta aataatcatc atccacataa tcagtgaaaa catgaaagac aaaattaata
     5161 tctgaattat taatcaaaac ggaagaaatt gaaacgccag caccatacaa aaaattctta
     5221 tcgataccgt aggaaacatt tagctctggg gcgttttctt ttttattatc tgctaaatac
     5281 gaaaaccgtt cttttataaa ttcattcatt taacttttct caatttcatt ttattatctt
     5341 taaataaaaa taataaatat aattcattat cccgttaatt ggtttatttt gtttaaaatt
     5401 atgcttcgcg caataacgag catagtttga gttaactggc cgcattaacg gttcattttt
     5461 ccagggcgat gcttctttgg ctttgataaa aggttgcgca cttggataac cggcccagta
     5521 atgccagggt tttgtcggac cgacataatg aattaatacg gtttcatcat taattggaca
     5581 aacaaatgat ttttttaatt cataatttaa actaaactgc gtattgtatt tagcatcaat
     5641 gagtttaact ttccctaaca ggataagatt aaggatatct tgatccatat aggttaaacg
     5701 ggaaacgatg gctttatcag caagcatcga catcgcttta gcagaaacgg actcctgcgc
     5761 ccacgctagt gtattaatta acaggacacc tgaattaaag taaccctttt caagttcatt
     5821 acactgcaga ctttgacccc gtaacgacca ccagttagca tcgcgttcag taacaacagc
     5881 agcaacgtta ttggcaagat ccattgttat cagcggcttt aagtttccct gacaggcgat
     5941 atcagcatcc aggtaaagga tcttatcttg ttgatcaata aagtaatctg caattacaaa
     6001 acggaaatac atggcaatag accaattttt ggtcgtcggt aatgccttaa gccgttcaca
     6061 atttaccaga tggatctgga tacaggtacg atagcttttc gccaattggg ctaaacgctg
     6121 gatatcggct tcagggatat catcaataaa aacgtggaaa acaaaactca cgtcgttgtt
     6181 atgtaacaaa actgacgtga ttgaaacacc acaaccaaaa agaaagtttt tatcaatgcc
     6241 ataagcaaca tggaatgatg atgccactga cgccgctggc ctttcatcga agatgattgt
     6301 cttattgatc atctcttgtg gattaaaata gtgggcactc atatttctct ccggaaatta
     6361 caacgatttt cgaatcgttc tttccctgat ttttgtggct ttttcttctg cttgcgacag
     6421 cagcccttgt tcctgcttga gaatgtcttt aagcggggcg acaaaataca ctttcataaa
     6481 ccgccagata tcccgctgag tcaggccgat attcatcgaa gaaaaataaa gcccaataag
     6541 atctttatcg cgccaacgac gtggaacgcg cgtgcgaagc tgcgcccggt gcaggtcaat
     6601 tactgaaatt tttaactcct cttccttacc ggaaaaaggc aagtgcagca ggaaatgaca
     6661 gatataacag tcacggtggt taatgcccgc agcatgcata tcgcgcacca tcgtcgcgac
     6721 acgcttaata agcatacgct ttacgcgaac atctggtggg ttggtcgccc agtcagcaca
     6781 gtaatcttcc agactgatgg ttggtgtcag atcttcggta ataataaacg aggttctggt
     6841 cagcggatta atgccttttt cgccgaatgc caccccatac atagtatcaa cgccgacatc
     6901 ccgcagtcga tgaatcgcat tccattcgcg gtctgcgcct aatactggca tccgcaatga
     6961 gagtaaattt ttgattatct ctttcagggt cgtgccgcga tgccatttga gaaaatagct
     7021 tttgcccgcc atttcaaagc gcagagtgcg gcgagtttcc agttcacgaa atacctcacc
     7081 ctgcaaggtt ttaacttcct caaaaggatc tttaccgcgc cataacgtgg caaacggctc
     7141 tttaagttca accatccaga ccacccgtta tgatgtccgc tgctttctct ggcagactgt
     7201 ataaatcttg tgtatcagca taatgtcgcg cattttctgc ccaggcctgg cgcaatgaag
     7261 attgcgttaa cgctttgcgt aaaatctcat tcaatgtttc ctggcggaat ggctcagcaa
     7321 tagcctcgcc gcaattggcg tcaacaatat aatgcgcata gccacaaacg gctgttgtta
     7381 gtaccggtaa tcctgcagta atcgcttcca gcagcacaat tcccgccgct tcctggtagg
     7441 caggatgaag taataaatcc gccgccgcca ttaattccga gacatcgttg cgccccgaga
     7501 agaagtgaac attactgcgc acgccgcgtt tttctgccag cgcctcaaat tttcgcggtt
     7561 tatcctgccc aacaacatat agtaatgtgt tgtggcgcag cgaatccggt aacgaagcaa
     7621 gtgcttcaat ggaacgatcg acacctttac gcgtgaagtc tgaaccgacc tgcaacaata
     7681 aatattgttg ttcggttatt ccattcttct tacggaagat ttcacggcta ttcgctggtt
     7741 gctggctata tttacgatca ggataaatcc ccggtggcag aatatgaaaa cgctccgctt
     7801 cagtctgata atgtttctgg aaatcggcga tttgcttatc tgtcagcatc agcagctgtg
     7861 tcggcttgcc ctgttcgaag gttgcccgct caaaggcggc ataatggcga taacgtgacg
     7921 tcaggcgata gaaaaagcct ttctcctgcg ctactttctc ggcataacaa acatcagcgg
     7981 cataataaac gtccagcccc ggcattttat taaatccaac gactctatcg acggggtatt
     8041 cacgtaaatg tttttgcacc caggcaaaat attccgcatt gcgcccgtga ttggtatgcg
     8101 atttaaccgg cactttgatc agttcaaata catcagggca ttcgccttcc cacgactggg
     8161 tataaacccg aacatgatga cctcgggcgg caactgtctg agcaatacgc ataaaatcgc
     8221 gctgcaaacc gccaaaggga aaatatttat ataaacaaaa agcaacgatc ataacgacgt
     8281 acctgtcgtg gaggagggaa gtaatttatc gacagctgca atgacatctg ccgccggaat
     8341 aaccgaaaga tacatctcat ttcggtcacg ctgatcgcgc gttggcattt cccggtaatc
     8401 tcccgcccag aattgaatca tgttatttga ccagggacgc cagaaaatat ggtctgtcgc
     8461 accaaacagc gatatcagtg gcgtattaac tgcagcggca atatgcgccg gtgcggaatc
     8521 aacgccaata aacagctgcg catgatcgat taacgcacca agttccggga aggtcacctt
     8581 tccggccagt gccgttactg gtggcgtctg gcatccctgc gcaatttcat tgacgcaggc
     8641 cagatcgtct ttatctgggc cggacgtcag aacgacttca taaccacgag catgtaaggc
     8701 atcaatcaca gcggaaaact tggcgttgtc ccagcatttg aagatttgcc gcgccgtagg
     8761 ttggataacc acataatttt gtccaacacc agcgtgatca agttcgcgac gcatacgttt
     8821 ccagcttgca ggcgggtaac tcatggttgt ctgcttcacc aacgattcaa gtcccaatgg
     8881 cgtcagcacg gataagttac tttccaccac atttccaccc tgcaacggca ccaaatgagt
     8941 gaaactatta cgccaaaaag cagactgccg atgatgataa tcctgggaaa ttttcacacg
     9001 ggcatttaat aagcgaacca gtatagcaac catccattga tcggtaagat taacgataag
     9061 gtcatactta ttggcacgta atatcttgat gagatggaaa aagttggcaa ttttttctga
     9121 ggcttttgct tttttatttt ttatgccgta gagcgcgtta atctctggat tttcagacag
     9181 gatcgggatg gtgtcctgat aaagcagcac atcgattttt gcgtcagggt aatttttttt
     9241 cagcgaacta atgacgggag tagttaataa catatcccca tgaaaacgca tcttaatgag
     9301 caaaattctt cgaaatggct tatccacaag taactctttt tgttgagatt atccgattaa
     9361 ggtgaagcag aaaaagcacg ctaccgcccc aggctcaaca gctacctgaa tactgatacc
     9421 gcactgatat tgacgcgcta gtgtatcact tctttcgcgg caatccgacc cgaatgattt
     9481 ttaagcacat tgttgatttt caataaagaa atcaatcaaa taaaatcatt agcaaccata
     9541 ttcttttact gttgctctgc gattccttcc tgcacgtact gcagaaatca atgaatcgca
     9601 taaaaataca aaaaaagtat agatgttcag caagaattgg cgggtaaatt gctatttaag
     9661 tcaaaaacgg gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc
     9721 cataagtcag ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca
     9781 gccgctgatc tggatacggc tctgggtgcg cggacgtaag gc
//
