LOCUS       NZ_CP070032             1551 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1337 plasmid unnamed10, complete
            sequence.
ACCESSION   NZ_CP070032
VERSION     NZ_CP070032.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357479
            Assembly: GCF_016903995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 909.52x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 60 of 192
            Pseudo Genes (incomplete)         :: 133 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            Pseudo Genes (short protein)      :: 4 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1337"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1337"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(9..485)
                     /locus_tag="I6K67_RS23600"
                     /old_locus_tag="I6K67_23600"
     CDS             complement(9..485)
                     /locus_tag="I6K67_RS23600"
                     /old_locus_tag="I6K67_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            895..1116
                     /locus_tag="I6K67_RS24395"
     CDS             895..1116
                     /locus_tag="I6K67_RS24395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158414302.1"
                     /translation="MCKCNTVTRDDIPFAVWSGLRPDTPHQCVRAQCGFRFSSASSPTR
                     SLRSVAGAAASGVTSRMLSVIPLLFFRG"
                     /name="hypothetical protein"
     Typing          565..673
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(876..956)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="87.0"
                     /evalue="9.59e-28"
     Typing          1070..1328
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="1.09e-111"
ORIGIN
        1 ccgctggctc aatttgaaaa catggccatg attcggcgct gaatatcacc gtctttgttg
       61 accagtgttt tagtgacaaa cgctgcatgg tttttcgtag tgttgtatcc cttccagact
      121 tcatgtggat cgagggcata cgcacaaacc ttaccagcaa cggggattct aactaatgca
      181 ttttttgatt ctaagtaatt gagccaccga ataattgtgc ttctgctaac tccaagtttt
      241 tcagctagga attgctgatc cgcaacgaca gcgccacacg taggatcgat atgctccgct
      301 aaaaatgagt acagcttcag tgctgatatg ccttgactat ccttcagcag ctctcgaacg
      361 cgttcccaac cttttgggga tacctgtgta aaccttgaat tcttctttgc ttcttcgaga
      421 tcctcggtgt ttttctttgc ctcatctgaa agttgtttca gtcgtttttc tgaatcaata
      481 aacatgattt tttccctctg aaaatcccat gcactaacgt tggttcaaat ctaagccatc
      541 atgacacatc tgtgcaacac cttgttgcgt gttgtcgcat ccaggatgcg acaacgttgt
      601 gtcaccctgg atgcgacagg taaacaaaaa acacactaaa aaaacaacaa cttacaaatc
      661 ggcgtaaaag atctattcta ggcaagggtg ttgcgcgcaa cgcaacgccc ttttggggtg
      721 tcggggcgaa gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttattccg
      781 ctacaagcct cgccagccgt gatttttgcc agatttcacg ctctcaacgc tcaacagctc
      841 catcatggtt ttgccgtccg ttcaccgcta aaacgcctca gaaatcggga caggatgtgc
      901 aaatgtaata ccgtcacccg cgacgatatt ccgtttgccg tctggtcagg gcttcgcccc
      961 gacaccccgc atcagtgcgt acgtgctcaa tgtggtttcc ggttctcttc cgcttcctcg
     1021 cccactcgct cactacgttc ggtcgctggg gctgcggcga gcggtgtcac ctctcgcatg
     1081 ttgtcggtga ttccgttgtt gttctttcgg ggatgagtcg ccagatagcc cctcaaacgc
     1141 gctcagaagc tctcagacac acgaaacggg ttttaaatgc ccgtcagggc atggaaggcg
     1201 cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct aaccaggggc
     1261 tttcaggtcg ctaggaaccg cgtcagcccc ctgcaaagcc tatttgcacg gcacaggcag
     1321 ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg tctggggttc
     1381 tccccagacg gtttccggtt gcgctcaagg cgctgccgga accgtggcgg cgcggtcgtt
     1441 ctggggatcg gggagcaaaa acaagcaagg gattttgagg cggcaaaaga gcaatgcagg
     1501 atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct g
//
