LOCUS       NZ_CP070031             2102 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1337 plasmid unnamed9, complete
            sequence.
ACCESSION   NZ_CP070031
VERSION     NZ_CP070031.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357479
            Assembly: GCF_016903995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 909.52x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 60 of 192
            Pseudo Genes (incomplete)         :: 133 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            Pseudo Genes (short protein)      :: 4 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070031.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1337"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1337"
                     /db_xref="taxon:562"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(1900..2102,1..763))
                     /locus_tag="I6K67_RS23590"
                     /old_locus_tag="I6K67_23590"
     CDS             complement(join(1900..2102,1..763))
                     /locus_tag="I6K67_RS23590"
                     /old_locus_tag="I6K67_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1630..1896)
                     /locus_tag="I6K67_RS23595"
                     /old_locus_tag="I6K67_23595"
     CDS             complement(1630..1896)
                     /locus_tag="I6K67_RS23595"
                     /old_locus_tag="I6K67_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(139..370)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcagctcaag aaaccactca ggatcgtttg ccatatcttc cggtttaacg gagtatttca
       61 gcgtttcagc aaccgcgctt tgcagttgct cggcaacgtt ggccacaacc tcacctgtct
      121 tagtttttac tgcccgaata tcgatattcg gctcatagtt cacccgcaag caatcgcgcc
      181 agagttctac ccaacgttcg tgcttaacgt agttcttccc tttaaaccaa gaaggttgca
      241 ccatcagcag acagtgaaaa tgcggatgtg cgctgccatc cttacctcgc gtcacctccg
      301 tagccctgat ccacccctga acaggtgata gctcctttcg cttttccatt cgcttaaacg
      361 ccgcattcat tgctgtaagg actgttccaa gttcacctat ctcgcagttc ctgacagtta
      421 acgtcagaaa caaccatcgg gaagacgggt aatccacaac gattttcggt agagcctgat
      481 aaaaacgggc ttgccacatg agggttcttc tccactggca gacagggcaa tgacggacac
      541 ggcaaaattg cgcactacga agccgtaggc gcgtttctcc ggtttccatg atggtagacc
      601 aaccaaaacg taacaaaccg ctacaggagg ccatacggag tgcatagcgc tcgaattcag
      661 cagcagtctg gtagataccc ccgacagaat cggtaagcgt gcgctgggta tcccaaactg
      721 catcacgggg gctgtagtcc gaaaggaatt tatcttctga cattaagcct gctccgatct
      781 gggcaggctg tgatttgatg tggtatattc atcacgtacc tgtgcattgc gagccagtac
      841 atgtcaccag agtttgccgc tgaggtgaca cattcgaaat gttggggagt gttgacgcac
      901 tccccaacgc cgattaaaaa cgcctcaatt ctgctccatt ttctttgacc tgtcaaaacc
      961 cactcaaagc ctcgtcatta ctgggctaaa ccactatttc atatccgcca tttatccctt
     1021 aagtataaat acaaggatca cgcgcgagaa ttgcgcaaaa aataaccaca attttaaccc
     1081 ccccaaaaca agcacccccg aacggcttac cccaaccttt tccacacacc cgatcaccaa
     1141 aacagggaat aaaaacaaaa ccctgtctta caggtggatg gcctcacttc gtgaaaccat
     1201 ccaccccgca taagtttttt aatcggtggt gtcgtcaatc actaaatcgg aaccccaaag
     1261 gggaccccca cgatttagca tttgacgaga ttaactctcg gagatggcat catgctttta
     1321 acggacttta aaaaacggtg aacggatgcg gattttaatc cgtgtctgtg aggcgttgag
     1381 caacgagcaa agcgaggcgg tagaaagaga gccagcggca ggctcaggac gataatccgg
     1441 ggaaaatgta ccactcgcag agtgttaaca tttgacatcg gattgtctat cctgcattgg
     1501 tgtcgagttg gcaaggctga ggataatgct gaggataatg ctgaggataa tgcaggatag
     1561 acagagcact agagattgca tgcaatctcg tacctatcac ctcagcctgc cgctggctct
     1621 tttccagaac taagaatgcg ttcttaaaaa tccaaacttg ataccgttta aatctatttt
     1681 cacaaaaaga gtttttaccc tctcgtttgt tttctgaata tataaaatta attcatcctc
     1741 aaaaccttcg tgcggatgaa aaccaaatgt gccaaaactt aatccatcaa taagcaaaat
     1801 aaggttttta aacctgtcag gaatcaccgc ttcacttctt aaaaaaaata aaccaatagt
     1861 cttatttcca ttcaggtatt caatattaat attcatacgt taatccgatt tatccttctc
     1921 aggggcgcgt ttgtaacgcc gtttacctga atcccagaca aacgccgtcc gcttcccgtc
     1981 atcagtgcca tcccctacat cgtcggcaat gacaagatcc tcattggttt ctcgatccaa
     2041 ctggaggacg ttttttagcg ccccaccggt cgagataaaa cggcgcttgt gaagctgccg
     2101 cg
//
