LOCUS       NZ_CP070029             4082 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1337 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP070029
VERSION     NZ_CP070029.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357479
            Assembly: GCF_016903995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 909.52x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 60 of 192
            Pseudo Genes (incomplete)         :: 133 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            Pseudo Genes (short protein)      :: 4 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070029.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1337"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1337"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(2793..4082,1..870)
                     /gene="mobQ"
                     /locus_tag="I6K67_RS23540"
                     /old_locus_tag="I6K67_23540"
     CDS             join(2793..4082,1..870)
                     /gene="mobQ"
                     /locus_tag="I6K67_RS23540"
                     /old_locus_tag="I6K67_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063610029.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCMNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1126..1827)
                     /locus_tag="I6K67_RS23545"
                     /old_locus_tag="I6K67_23545"
     CDS             complement(1126..1827)
                     /locus_tag="I6K67_RS23545"
                     /old_locus_tag="I6K67_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2360..2614)
                     /gene="mobC"
                     /locus_tag="I6K67_RS23550"
                     /old_locus_tag="I6K67_23550"
     CDS             complement(2360..2614)
                     /gene="mobC"
                     /locus_tag="I6K67_RS23550"
                     /old_locus_tag="I6K67_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2616..2792)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2793..3151
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1127..1827)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..867
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2794..4082
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.07"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 aatgccgtta tggattcagg ggtactgagc gcagtcgtta cggggtatgg cgaggccccc
       61 atgcctggta acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatac
      121 acgctctggg ggaacgagct ggagaaatac cgcaccagaa tctttgagcg tgttgattta
      181 atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa tcggtcaaca tccaccactg
      241 caacgtgttt attcctctgc gacctttgag caacttctga cgcaggtttg ccagacatgg
      301 ccgcaatata cccgcaatct ccgccagccg aagacatggc cggagtcttt ctgtcttgga
      361 gaagaccgcc agccagccat gccatcactg gcagcaagga aggtggattt tacgcaggga
      421 cgtctgctac cgacgctgat gccggtaatg agttctgtcg accgggaaac ccgccagctt
      481 cagcttttgc tggtcatggg cgtggatgac tctctgggcg gtgttgtccg gcttaatggt
      541 acgctgtatc ccgcgtttgc cgttccctca gccgataaca gccagctggt catcagtgca
      601 ctgaccgata aggggttacg gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac
      661 agccacatat cacctgcgcc ggaacttatg gagtttcatc tgaaaacccg tgaggcaccg
      721 ctttttgctg ccgtcaacac accagaaaaa cagccggatc atctgttccg ctcgctgggt
      781 tttaatcgta cctgggacga atggcggcgc gaggaagatg cacgaacaca cgccaccgag
      841 aggagacatg atcgcggttg gtcacagtga atgtgaacaa tattttgttt tgtttaacat
      901 cgtgttatgt tcgtcgatgt tcttcgatgt tcatcgacag ggatgaatgc aagaagcaca
      961 cacataatgc aagcatagtt ttgtttttcc gcccccaaaa aatgggggtt cttttttcgc
     1021 tgcattagag cgcttttgac gcaatttttc acttcaatag taccttcgca ctactaacac
     1081 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
     1141 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
     1201 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
     1261 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
     1321 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
     1381 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
     1441 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
     1501 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
     1561 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
     1621 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
     1681 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
     1741 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
     1801 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
     1861 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
     1921 atattacctt aaatcatatt gcctttacct taaattcctc cacctaaaca ccttaaatca
     1981 tattaccttt accttaaatc atattaccta tagcgttgta acattttgtt ttctataagg
     2041 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     2101 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     2161 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     2221 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     2281 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     2341 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     2401 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     2461 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     2521 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     2581 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     2641 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc atggaaaatc
     2701 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     2761 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     2821 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     2881 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     2941 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tatgaaccgt gccgaactct
     3001 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     3061 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     3121 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     3181 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     3241 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     3301 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     3361 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     3421 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     3481 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     3541 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     3601 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     3661 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     3721 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     3781 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     3841 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     3901 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     3961 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     4021 agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc aggaaaaatg
     4081 ac
//
