LOCUS       NZ_CP070019             1668 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1309 plasmid unnamed4.
ACCESSION   NZ_CP070019
VERSION     NZ_CP070019.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357451
            Assembly: GCF_016903955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1209.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:53:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,448
            CDSs (total)                      :: 5,325
            Genes (coding)                    :: 5,168
            CDSs (with protein)               :: 5,168
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 57 of 157
            Pseudo Genes (incomplete)         :: 101 of 157
            Pseudo Genes (internal stop)      :: 26 of 157
            Pseudo Genes (multiple problems)  :: 23 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1309"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1309"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(603..797)
                     /locus_tag="I6K39_RS27095"
                     /old_locus_tag="I6K39_27095"
     CDS             complement(603..797)
                     /locus_tag="I6K39_RS27095"
                     /old_locus_tag="I6K39_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041620.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1081..1404
                     /gene="mobA"
                     /locus_tag="I6K39_RS27100"
                     /old_locus_tag="I6K39_27100"
     CDS             1081..1404
                     /gene="mobA"
                     /locus_tag="I6K39_RS27100"
                     /old_locus_tag="I6K39_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_204779902.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIAGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          833..1072
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(45..572)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.071"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          1082..1401
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.42e-63"
ORIGIN
        1 gacttttacc aatggctgct gccagtggcg ctttgtggtg tcttttccgg gttggaccca
       61 agagaacagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      121 cccagcttgg agcgaacgac ctacaccgag ccgagatacc aacagcgtga gctatgagaa
      181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      301 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gctcgtcagg ggggcggagc
      361 ctatggaaaa acgcctgcgg cgctggcttc ttccggttcc ttgttttttg ctcacatgtt
      421 ctttccggct ttatcccctg attctgtgga taaccgtatt accgcttttg agtgagctga
      481 caccgctcgc cgcagtcgaa cgtccgagcg tagcgagtca gtgagcgagg aagcggaaga
      541 gcgccttatg tgacattttc tccttacgct ctattgtgcc gttcggcatc ctgtcctgag
      601 cgttatctct ctgtgctatt ttctacttca aagcgtgtct ggatgctgtt ctggagttct
      661 tctgcgagtt cgtgcagctt ctcacacatg gcagcctgtt cgtcagcatc gagtgcgtcc
      721 agcttttcga gcagcgtcag gctctggctt tttatgaatc ctgccatgtt gagtacggct
      781 tgctgctgct tgttcatctt ttcgttttct ccgttctgtc tgtcatctgc gttgtgtgat
      841 tatatcgcgt accacttttc gactgtttta ccgccggtat tctgccgtct gaagctttga
      901 cgggtatttc tgcctgacaa tactgtcact gccaaaaaac tgccgtgcct ttgtcggtaa
      961 ttcgaacttg ctgacaggac aggatgtgta attgttatac cgcgcataca tgcacgctat
     1021 tacaattacc ctggtcaggg ctttgccccg acacccatgt cagatacgga gccatgtttt
     1081 atgacaaaac gaagtggaag taatacgcgc aggcgggcta tcagtcgccc tgttcgtctg
     1141 acggcagaag aagaccagga aatcagaaaa agggctgctg aatgcggcaa gaccgtttcc
     1201 ggttttttac gggcggcagc tctcggtaag aaagttaact cactgactga tgatcgggta
     1261 ctgaaagaag ttatgagact gggggcgtta cagaaaaaac tctttatcgc cggcaagcgt
     1321 gtcggggaca gggagtatgc ggaggtgctg atcgctatta cggagtatca ccgtgccctg
     1381 ttatccaggc ttatggcaga ttagcttccc ggagagaaaa ctgtcgaaaa ctgacggtat
     1441 gaacaccgta agctcccaaa gtgatcacca ttcgctttca tgcatagcta tgcagcgagc
     1501 tgaaaacgat cctgacgcat ccttcctgtt tttccggggt aaaacatctc tttttgcggt
     1561 gtctgcgtca gaatcgcgtt cagcgcgttt cagtggtgtg tacaattaag ggattatggt
     1621 aaatatatga gctatgcgat aactttaact gtgaagcgat gaacccat
//
