LOCUS       NZ_CP070018             2804 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1309 plasmid unnamed3.
ACCESSION   NZ_CP070018
VERSION     NZ_CP070018.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357451
            Assembly: GCF_016903955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1209.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:53:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,448
            CDSs (total)                      :: 5,325
            Genes (coding)                    :: 5,168
            CDSs (with protein)               :: 5,168
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 57 of 157
            Pseudo Genes (incomplete)         :: 101 of 157
            Pseudo Genes (internal stop)      :: 26 of 157
            Pseudo Genes (multiple problems)  :: 23 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1309"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1309"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            163..1089
                     /locus_tag="I6K39_RS27080"
                     /old_locus_tag="I6K39_27080"
     CDS             163..1089
                     /locus_tag="I6K39_RS27080"
                     /old_locus_tag="I6K39_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_204779901.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLKEIKKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1092..1625
                     /locus_tag="I6K39_RS27085"
                     /old_locus_tag="I6K39_27085"
     CDS             1092..1625
                     /locus_tag="I6K39_RS27085"
                     /old_locus_tag="I6K39_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1691..1900)
                     /locus_tag="I6K39_RS27090"
                     /old_locus_tag="I6K39_27090"
     CDS             complement(1691..1900)
                     /locus_tag="I6K39_RS27090"
                     /old_locus_tag="I6K39_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          164..1086
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.078"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggtgtttt tttgcggttt gttgtggtcg tgcagtatat cgcttgcggt cggcgggtgc
       61 agggggtatc tttcgtgcta attcgtaaaa ttagaaggaa gcgcacttat atgtttctct
      121 ccttcgtcga gaaacagtgc gctcctgcag agggctgaaa ccgtggcaac ctatcacctc
      181 agcgttaaat ttggtggcaa ggggcaggct gcaaaccacg ccgattacat cgagcgcaaa
      241 gagaaatacc gcgaccgtca ggatttggaa tattccgcac acggaaacat gccggaatgg
      301 gcgcgagata atccctccca tttctggcag gcggctgacc agtttgaacg tgcgaacggt
      361 tcgacctata gggaacttga aattgcatta ccccgtgaac tgacaccgga acagcgcctt
      421 gaactggtgc aggattttgt acgtcaggaa gctggcgaac ggcacgcctg gagttttgcc
      481 attcacaatc cgaaagcgag cattgacggc ggcgaacagc ctcatgcgca tatcatgatg
      541 agtcagcgtg tgaatgatgg cattgaacgt acaccggaac agtatttccg gcgctataac
      601 gccaggtatc ccgagcgggg aggtgcgaaa aaggattccg gaagcctgac tctcactcag
      661 caaaaagagc agttaaaaga aataaaaaaa cgctgggagg tgaagcacaa cgaacatatg
      721 agaaagcatg gctttgaaag gggcttcatt gactgccgga cgctgaaaga acagggcatt
      781 gaacgacgtc ccgaagtgca tctgggtttt gaagctgccg gacggcttga tgatgcccag
      841 cgtcatgaca tcatgcaaaa acggtctgaa ccggactatt cccgccatct tgaacaggcg
      901 cgaacggtcg ggcatgacat cagggaatgg ctggacactc cggttaagca acatgaactg
      961 gtcagaaggc tgcaacatga tgttgcgcgg gagcatggct ttgacatgag agacagagtg
     1021 agcagacaga accgaactgc acctgaacgg gttaaatcac cgtcacgtgg attttctatg
     1081 ggattctgaa catggccagc acagaagaaa aattactcac gctcctgtta tcggtggagc
     1141 atctgcaaca taccgcaatg gagcaacaga aagctgttgc tgattctctg gctgtggcaa
     1201 gggaaactct tcgcggatat cgggatattt ctgacggtgt ggcaaaacag gtcagggatg
     1261 aagtcagaaa tgaggtgcag aagctggacg tggcaggact gataggtgag cgcatcagcc
     1321 aggattttca gcggctggaa gagtcagtcg caaacatgaa ggcaaacgcg gatgccctga
     1381 acagggatat tgcgacaatt cacagcaata tcgtgcagga atacaacacc cttaaagggt
     1441 atagctggaa atggctgggt ggggtgtcag tggcttttct ggttatgttc tgtgtgctcg
     1501 gatggggaat gaaaacaata ctcgacagga actacaacgc cacagcggcg gtgtatcaga
     1561 aagttgtgac actggaagat aaactggtgg agaaacctgt cacggggaag agccggaaaa
     1621 actgacgcgc ttcgcttgtc caacgcctca cgacccgcga aaggatttcg cgaaccgttc
     1681 accgtttttt tcaatatgat tctgtctgct ttggtcgata tagcagacga tagtattcca
     1741 gccttcgtat atataacggt acgtgctcat cagtaagagc tggcggtatt gtgttatcag
     1801 ggggaagttt tttcagatac tcacgaaatg cctggcttga tgccataaaa tctacagtag
     1861 catcgataat atgtggggtg ttttcaccta attttgccat gatgataatc cttatattat
     1921 cacggtttgt gaatgatagg gtgtcactat tatttatgcg ttagtgaacc agaaaaaatc
     1981 ccccgttggc atagggggat atgtaaggag aaataacttc gcaggtgttc ttgtttgtat
     2041 aacgtgaaaa gactgcacgc aacttaataa gtctgccgta cttactttca gttttcaaca
     2101 taaggtttat aacaatgtat ggaatggaag tgagctttaa ggtttcagct ctaaaaaata
     2161 aaatatttat tagttggctg gttgttgttc ttgttctgta tgtatataaa cactatcgac
     2221 atattatgac cgggaacatt ccttaccccc tctgcgctcg cagaggtttt ttttgtcatt
     2281 aattaataat gaactgaaat gcagttccga tacctccatt tgcacattat ccctgacata
     2341 tggtaccgga accgcattaa ttttatgtta taagagttta tttttaatgt ccagatggtt
     2401 taatagtatt tatatgtaat tagctgtatg gatagtactg gttttattgt ataacttgtc
     2461 gtttttttta ttgtttttgt gtggtgtgct aatctttaag ttataaaaaa accccttgga
     2521 atacaggggc aaaagtaagg aaaacaaatg gatgctcagt ttgtgtgaca ctttaaacta
     2581 atggaactat agagtggagg ttgttacagt cgcttttata aaacagcaac cagttttttt
     2641 caatagtctg atgatggttg tgacagtttt gtgatattgt tcaaaatgtt tcttgttgaa
     2701 tgtttgtttt ttggtctatt ggtgtctggt tttatttttt ctgctattac atatcaagaa
     2761 atgtgattgg tgtagcaaga gtggaatgcg atgtggataa acta
//
