LOCUS       NZ_CP070010            15883 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1288 plasmid unnamed3.
ACCESSION   NZ_CP070010
VERSION     NZ_CP070010.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357430
            Assembly: GCF_016903935.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15883)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1384.73x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:06:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,672
            CDSs (total)                      :: 5,545
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 67 of 217
            Pseudo Genes (incomplete)         :: 153 of 217
            Pseudo Genes (internal stop)      :: 34 of 217
            Pseudo Genes (multiple problems)  :: 36 of 217
            Pseudo Genes (short protein)      :: 4 of 217
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1288"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1288"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..1366)
                     /locus_tag="I6K18_RS27285"
                     /old_locus_tag="I6K18_27285"
     CDS             complement(<1..1366)
                     /locus_tag="I6K18_RS27285"
                     /old_locus_tag="I6K18_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_251301812.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEAR
                     LVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRLQA
                     YVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRSWSV
                     ALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGSNNLN
                     NVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITRNSKGW
                     LNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERTAVAGAS
                     CSPNGLVGRDNTGAILSCQSGTWRKSNSGSTVITGRIANGQQIPLPTGFSASQCSWSVS
                     NAENPQGWKPNYFAGSVATYDANRIVKCGFYDEYNFHKGTFRADLTGKCSYVVACQ"
                     /name="shufflon system plasmid conjugative transfer pilus
                     tip adhesin PilV"
     gene            complement(1366..2022)
                     /locus_tag="I6K18_RS27290"
                     /old_locus_tag="I6K18_27290"
     CDS             complement(1366..2022)
                     /locus_tag="I6K18_RS27290"
                     /old_locus_tag="I6K18_27290"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="WP_001193553.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWYK
                     THPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFTRR
                     FLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITAWSG
                     LYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI"
                     /name="prepilin peptidase"
     gene            complement(2007..2567)
                     /locus_tag="I6K18_RS27295"
                     /old_locus_tag="I6K18_27295"
     CDS             complement(2007..2567)
                     /locus_tag="I6K18_RS27295"
                     /old_locus_tag="I6K18_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000014116.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAAA
                     GARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLINMG
                     VIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYANKI
                     WRIYRDMKGICLPGQGGRQCRQS"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(2577..3191)
                     /locus_tag="I6K18_RS27300"
                     /old_locus_tag="I6K18_27300"
     CDS             complement(2577..3191)
                     /locus_tag="I6K18_RS27300"
                     /old_locus_tag="I6K18_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="WP_000959785.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVLG
                     SLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGMTV
                     QGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTGLTN
                     GITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
                     /name="type 4 pilus major pilin"
     gene            complement(3209..4306)
                     /locus_tag="I6K18_RS27305"
                     /old_locus_tag="I6K18_27305"
     CDS             complement(3209..4306)
                     /locus_tag="I6K18_RS27305"
                     /old_locus_tag="I6K18_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="WP_001208805.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTAL
                     EQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGIRS
                     GSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKISSP
                     DSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKIMPWS
                     IYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGDHLGLA
                     LRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRLIMLIFL
                     VMSLMLLVFAIMDIQSISDNSMGNF"
                     /name="type II secretion system F family protein"
     gene            complement(4319..5872)
                     /locus_tag="I6K18_RS27310"
                     /old_locus_tag="I6K18_27310"
     CDS             complement(4319..5872)
                     /locus_tag="I6K18_RS27310"
                     /old_locus_tag="I6K18_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_000362202.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLYP
                     DINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGASDI
                     HFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQDARL
                     SPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLNIMLR
                     RPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTPIICDK
                     SDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTLHTNSAL
                     GIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDDERDYLER
                     HCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEVIEPDNRLF
                     QILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDEDERLSIDDV
                     "
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(5883..6335)
                     /gene="pilP"
                     /locus_tag="I6K18_RS27315"
                     /old_locus_tag="I6K18_27315"
     CDS             complement(5883..6335)
                     /gene="pilP"
                     /locus_tag="I6K18_RS27315"
                     /old_locus_tag="I6K18_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="WP_001247336.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQAK
                     VQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKDKR
                     LNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
                     /name="pilP"
     gene            complement(6322..7617)
                     /gene="pilO2"
                     /locus_tag="I6K18_RS27320"
                     /old_locus_tag="I6K18_27320"
     CDS             complement(6322..7617)
                     /gene="pilO2"
                     /locus_tag="I6K18_RS27320"
                     /old_locus_tag="I6K18_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="WP_000752774.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHKP
                     HVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGVFR
                     YSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHPDNW
                     ESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTPEEIA
                     ARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTCTPETF
                     TLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEAVPTPSE
                     QLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVNPDELFPL
                     FQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
                     /name="pilO2"
     gene            complement(7610..9292)
                     /locus_tag="I6K18_RS27325"
                     /old_locus_tag="I6K18_27325"
     CDS             complement(7610..9292)
                     /locus_tag="I6K18_RS27325"
                     /old_locus_tag="I6K18_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA];
                     GO:0009306 - protein secretion [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="WP_000748143.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSAH
                     AREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQELG
                     QRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTSTQP
                     LTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSSSSSV
                     SSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGRYWLSA
                     SSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDWNLVYKS
                     LHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDVSVVTSQE
                     STVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLLPLIQKTGN
                     LQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLVVTGFDQNNT
                     TTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
                     /name="PilN family type IVB pilus formation outer membrane
                     protein"
     gene            complement(9306..9743)
                     /gene="pilM"
                     /locus_tag="I6K18_RS27330"
                     /old_locus_tag="I6K18_27330"
     CDS             complement(9306..9743)
                     /gene="pilM"
                     /locus_tag="I6K18_RS27330"
                     /old_locus_tag="I6K18_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="WP_000539807.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQILM
                     IANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETLRS
                     KSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
                     /name="pilM"
     gene            complement(9743..10810)
                     /gene="pilL"
                     /locus_tag="I6K18_RS27335"
                     /old_locus_tag="I6K18_27335"
     CDS             complement(9743..10810)
                     /gene="pilL"
                     /locus_tag="I6K18_RS27335"
                     /old_locus_tag="I6K18_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="WP_000742600.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISESA
                     GVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKPGS
                     APVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLNRLL
                     EEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQNKPRN
                     PFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLRENIIKWA
                     EETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKPLYAQAS
                     RMQCIITVSDTRDGR"
                     /name="pilL"
     gene            complement(11484..11936)
                     /locus_tag="I6K18_RS27340"
                     /old_locus_tag="I6K18_27340"
     CDS             complement(11484..11936)
                     /locus_tag="I6K18_RS27340"
                     /old_locus_tag="I6K18_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000008892.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFLI
                     IMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIENSIISRETTDDESDINT
                     LYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
                     /name="hypothetical protein"
     gene            complement(11979..12233)
                     /locus_tag="I6K18_RS27345"
                     /old_locus_tag="I6K18_27345"
     CDS             complement(11979..12233)
                     /locus_tag="I6K18_RS27345"
                     /old_locus_tag="I6K18_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001136192.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNCL
                     VVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(12501..13067)
                     /gene="traD"
                     /locus_tag="I6K18_RS27350"
                     /old_locus_tag="I6K18_27350"
     CDS             complement(12501..13067)
                     /gene="traD"
                     /locus_tag="I6K18_RS27350"
                     /old_locus_tag="I6K18_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TraD"
                     /protein_id="WP_000776428.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIALP
                     LLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKLPP
                     LLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRILSQ
                     WKVPHINPRCGIVILTLKNDSGNRE"
                     /name="traD"
     gene            complement(13081..13764)
                     /locus_tag="I6K18_RS27355"
                     /old_locus_tag="I6K18_27355"
     CDS             complement(13081..13764)
                     /locus_tag="I6K18_RS27355"
                     /old_locus_tag="I6K18_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="WP_000213857.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSGA
                     SRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKLQK
                     AGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITPLHR
                     KTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGATQEQT
                     TITLH"
                     /name="conjugal transfer protein TraC"
     gene            complement(14018..14551)
                     /locus_tag="I6K18_RS27360"
                     /old_locus_tag="I6K18_27360"
     CDS             complement(14018..14551)
                     /locus_tag="I6K18_RS27360"
                     /old_locus_tag="I6K18_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_001303319.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITRK
                     IRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNKDI
                     VDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLELVSN
                     AEHHGFMERLVNIP"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            complement(14993..15280)
                     /locus_tag="I6K18_RS27365"
                     /old_locus_tag="I6K18_27365"
     CDS             complement(14993..15280)
                     /locus_tag="I6K18_RS27365"
                     /old_locus_tag="I6K18_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="WP_000074849.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQN
                     CRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
                     /name="conjugal transfer protein TraA"
     gene            complement(15198..15476)
                     /locus_tag="I6K18_RS27370"
                     /old_locus_tag="I6K18_27370"
     CDS             complement(15198..15476)
                     /locus_tag="I6K18_RS27370"
                     /old_locus_tag="I6K18_27370"
                     /inference="COORDINATES: protein motif:HMM:NF022029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="WP_219065241.1"
                     /translation="MWRRFLPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWA
                     ITNERRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
                     /name="ash family protein"
ORIGIN
        1 tctgacacgc gacgacataa ctacattttc cggttaaatc agctctaaat gtccctttat
       61 ggaaattgta ttcatcataa aaaccacatt taacaattcg atttgcatca taagttgcaa
      121 ctgatcctgc aaagtaattc ggcttccaac cttgagggtt ttctgcattg ctcacactcc
      181 atgagcactg actggcagaa aatcctgttg gcagcggtat ttgctgacca tttgctatac
      241 gaccagttat caccgtacta ccagaattgc ttttacgcca agtaccggat tggcacgaaa
      301 gtattgcccc tgtattatcg cggcctacaa ggccgttagg cgaacatgat gcgccagcaa
      361 cggcagttct ttccagttgt aagtattcac cagtataaag gcgaccatca gcccgaacag
      421 taccgccttt cacctgaccg ccggtataga tgcccttgtt gtttacactt cgaacccatg
      481 atccatcgga catataaaat ccgccaccgt gagtttcatt gagccagcct ttactgttac
      541 gagtaattaa ccaaccatta ttacttctga tatcaccgcc agcggtgaca tttacgtcaa
      601 agtttccgct attgcctttt acctgaccgc tgaaagtgcc atttacacca ttcacattgc
      661 cgctgaaatt acctgtctgg gcatttactg ccccaacgtt attcaggtta tttgatccca
      721 tatcaatggc cgtgtgcatt ttgtttaagt cagggcgacc attgacctgg aatctgtaaa
      781 gacgatcagt gtcctcagct gcaccactaa gttcatctgt cgataacaat acggcaatat
      841 gcccgttacc gcttttggca ccataattac ttaaagctac tgaccaggaa cgtaatgcac
      901 ctgtggctgt tttgccgtcc tggatatatc caccaagacc agtggtaata tccttagcca
      961 tctggataag tgctttcact ggataaggtg tgccaccact ggatacaacc attgcctgta
     1021 gtaattccgg gttttgggcg tttcgaacca catatgcctg taaccgctgc ccctcgctgt
     1081 ttgtctcagt aaacccgctg gacaaaaagc cggtattttt cagcatggtt gtcgtgataa
     1141 cggcaggagt tgttgtggta ctactgccct ggagagtcgt gtagtttttc cctatataag
     1201 agcgggccgc actggtccag ttgctgacaa gacgtgcttc agtttgccat ccttttgttt
     1261 gaatatagtc ctgccatatg cccgctcccc aggcaatcag gagcataaca atcagtaaag
     1321 ccgcgccagt ttccagcgat gcccagcctc ggtcatattt tttcatcaga ttacccatag
     1381 tggttgataa agattatcca gcagcaaaag gacatggcca aaacatagcc atggacccag
     1441 agggccttct ttatgccctt ttatgcacca tgttgaatgc cataagacaa atcctcctgt
     1501 gcccagcaac acacaccata tggcgttata taaccctgac catgcggtaa tacctgcaat
     1561 aagccacagg tctccagtgc caatattaag acgatggcgg ttaactaatt tatgaaggca
     1621 gaataagaca attgcagcag tggcaaattc tgttgtacgg aaccaccata tatcagtcgt
     1681 gatttgtgat agcattcctg caattaaaaa acgtcgggtg aatgttcctg gtaataaacc
     1741 tgtcagtgca tccgttaccg ccatttgcag tatgaacgaa agaataagga cagcatgaat
     1801 acggtagagc agtggtgatg atgataaaat aattaacgcc acagctatac tgtatagcca
     1861 tattcctgac agaacagcag ggtgtgtctt ataccagaga tgtcccgtat cagataaaaa
     1921 ataacgaact ctgaatagca gagttcgtcc aataatggaa aatgtgaaca tcaatggcag
     1981 tatataaaca ggggatagac ttatgcctac gattgtctgc attgcctgcc tccctgtccg
     2041 ggaagacata tccctttcat gtcacgataa attctccata ttttattggc atattgctct
     2101 cttgtttcat gtctgtcctt acggaatccg gcattgtatg acccaaggca attccagctg
     2161 acgccacata tctgaaaatg tcttgccagt atccagcttc ctatatggat attaaggcat
     2221 ggtttggtaa ttaaatcctc agactttttt attacgccca tatttatcag ctttggaata
     2281 tgggttgagt ttatttgcat caacccataa tccgtgctac tggctttacc tgtttttttg
     2341 tccttattta tattaattgc gcccggcctc aacgaagatt ccccggcaga aatagccttc
     2401 aataataatg gttcaatttg atagcgcgcg ccggcagcgg caaagcaatt gtcccacttg
     2461 ttggtccaga tctgtttggg agctacgtat gaagctcggg cgggtggaat aagagaaaaa
     2521 agcaggatta atgaaataag tctgaggggt actgtacccc ctgacattaa aactccttaa
     2581 ccattgatgg tgaagataag tttgtttgta ccagtgctgc cattatctgc cttgcattgc
     2641 gtactggctt cttcagtagt gacttttcca tcactgtgag cagtactgtt cagggtaatg
     2701 ccgttagtta aaccagtttt actgattcgt gtggcaatct ggatacaggc atcctgtggg
     2761 actttatcat aggtaacggt aaaaccgttg ttgaaccctg atgttgaagc cggggcaaca
     2821 gttacagcac cgccccatgc gttatagagc gttgctgtac cagatgtttt gtcgccctgt
     2881 acggtcatcc cagagggaat gacacccatc tgaatgaggg cacctgtcat tttggcactg
     2941 ctggtaaatg tatatccatc gctacctttt agcaggctct gggcacttgt gataattgtt
     3001 tgtatatttg cggtttcagt tgccacattg gctcttgtac gcaacgcata caaactaccc
     3061 agaacaacga ctataacaaa aagaacaacg agcgctattg ttccctgctc caaaattgcc
     3121 catcctttat catgaggctc attcttctta ttatttccag ttaaagtggt attaatattt
     3181 tctactaaca taaatatcct cgttttgttt aaaagttacc catgctgttg tcactaatag
     3241 actgaatatc cataatggca aaaaccagta acatgagaga catgacaaga aatatcagca
     3301 taattaaacg aatcacgttt gcccgtttct ttacacgttg aagtgtttga ctaagccatc
     3361 gttgaccata attgctgatt agctctgttg cgccatctcc ctgtaataaa gacaggaagt
     3421 tggctgcttc cctggatggg aactgataac cgcactgacg caaagccagt ccaagatgat
     3481 ccccctggcg gacgcggtaa ataatgctgt caagacgtgt gcttaaccat ggtgatgcaa
     3541 actcctgaag aatgtttagc gagttgagcg tcgtcatttt ggctttcaga agtgcagcca
     3601 tattcaggag aaatgttgct ccctgtatat cctgataaat actccatggc attatcttgt
     3661 cggcaaaagt acgaacactg tcagggcttt tccagtttgg cagagaccat gatattaaac
     3721 ctgtgatcac tgcaaaaaga acggcacata tagcgccgta attatcgaca aaaacagata
     3781 atccataaag aaaaccaaga gcaccactcc atgaatcggg ggagcttatt ttgctcagtt
     3841 caggtattag ctctgtattc agaacatata atgtccccgt catcatgatt aacaatccca
     3901 ccggataaat agccatttgc catatagcct gatggatttg ttcttttgca tctgtaagag
     3961 tggtggcgaa ttgcagtgca tccacaattg aaccacttct tatcccggcg ctgattactg
     4021 cggcctcttc ctgaggaacc cataaactaa gagtatattc cagagagttt tcgccactgt
     4081 tttcacgaag agactcaatg cagtctgtgg ccagttcagc aaagggatgc cattttcgtc
     4141 caaagtctgt ccaggcatcc cgcatctgtt ccagtgcggt ctttaatggc tgtttatttt
     4201 ccagcagaaa acgcagggct tcgtaaaact gcaccctgta gggggcgctg aacgtttttc
     4261 tgacaatgaa acgcctcagc cgttgactga aattcatttc acgcatgttg ttctcctgtt
     4321 agacatcatc gatgctgagg cgctcgtctt cgtccagagg gatgattctg tcggcttcaa
     4381 gagggtctac cagaccttca ttgatacggc gaagaagatg ctctacacga ctgatgcctt
     4441 tcatgttttc cagccagtat ttacgtgctg caactttgcc gcgtgttttc agaatctgaa
     4501 ataagcggtt atcaggttct atcacctcag caattacagt cctgccggtg aggcctttac
     4561 ctatttcacc gcgctttctg ccgttaatga ttacgtcgtg attacattca gagcatccgt
     4621 gatgatttcg gaaccagata ttatctgtac tgcagagtga atctttattg cagtgccgct
     4681 caaggtaatc acgctcgtcg tcgctaagtt caggtgctcg cttttcccat ggaatacggc
     4741 aggagggaca cagagttggt acaaggcgct ggctgatcat cccaatcagt aattgcgcat
     4801 cgcaaataag atcggcattc atacccatcg taatcattcg ctccggtata cccagagctg
     4861 agtttgtatg taatgtcgtc agaactatgt gcccggtctg tgccgcgtag gtggttgaca
     4921 tcatcgagat aagatcgcgc atttcccctt ccatgatggc atcagggtca agtcgcattg
     4981 ccgatgaaat agcccgactc catgccagtt tgacggcatc ttcgtcggat ttgtcgcaga
     5041 taatcggagt ctgtgttgct cccagtatct gtccttccag cggatcttcg attgttagca
     5101 ggtgtcgccc ctgattatcg tcaagatata cacggcaggc gctgcgtaac gttgtcgatt
     5161 taccggaacc ggttggacct gacaggacta tttttccttc cgggcggcgc agcattatgt
     5221 tcagcaaccg gatttgttcc gggataaagc ccagttgttt aaacgtggga actttatctc
     5281 cgtcatcggg tatcaggcgc ataactgcaa tcagaccgtc tcccgtaggt ctgtgactgt
     5341 agcgtgcacc aaatattcct atttttcgca ttaattgtgg ggacaggcgc gcatcctgct
     5401 ccctttgagg gaagaaactg gtttcagtga catcagccat tgacagaatg gctgttgcgc
     5461 aaagtgaata gcccagtgct ggttgatctt catctacagt ctgcagttca ccgaatattc
     5521 tcatgcgaac tttgaaaata gactctgaaa ttaagaaatg aatatctgaa gcaccaagtt
     5581 ttctggcagt ttcaaaataa gatagaactt tcttctcact ttcactgaca tcagcaaggt
     5641 ctttcagatg aataattcgt tctttcgggc ctctgttttt ctcatcctga cctgcctcct
     5701 gtaactccga caacgagact acttttgggt tgatatccgg atataatttt agcagctcct
     5761 ggagattact ttgtacgtca ggccttgtcc gctggtttgt ttctataaga atttcatggg
     5821 tgtctcccct ggatataaaa aggagagcat ccttcagatt atatttatcc atatcacgtt
     5881 actcagaaag taagggtggt gccatcactt aacgtgacgc cagacaatga gatcgacttc
     5941 actgtgacgg atgtgcctgg aagctggctg ccagtggtga cgcttgtctg acgtccatca
     6001 gccatgcgaa gaacggcatt aagacgttta tctttgccgt ttatttccat aataacgggc
     6061 aattcctttc tgtttttaga tgtcggctgc tctgaaactg atggcaaaga ggcagtcaca
     6121 ccagagaaac ctgaaacagt ttcagatgat gaagtaacat ctgattcttc gagttgtttc
     6181 tgtagctgtg cgccctgaac ttttgcctgt aaaagaatat tccggttttg ttgtgcttca
     6241 agttctccta ttgtgacaag aggttgtgtt gttgcaaatg aaaaaccgga gaagaaaaat
     6301 atggatggaa taatgagtaa tttacctgga cgcataaata tgaccttctg aggaataact
     6361 gaaagtaccg ccgtttaatt caaaatggat attgctcaga cgaacgccgg tatcctgaaa
     6421 taaagggaac aactcatcag gatttacagg cgttgataaa ctgaactgat attctttcca
     6481 tttctgaaca ggggctggtt caccatcgtt acctggtaat ggttccggta tggctatttc
     6541 attaatggcc ggcgttaact gttttttctg aaaccacgtg aacacccgca tcagttgttc
     6601 tgagggggta gggactgcct catccctgcg gggcagggaa ggcagtggcc gggttacaga
     6661 agccaggcgt gcaccatctt taaggttaaa gtcaggaata acattaaaga tttctttgct
     6721 tcttgccaga aagccttcaa tagttcctcc tggctgtcgt tcgtaaatca gtgtgaatgt
     6781 ctcgggagta catgttccac cggtaagttt ccagccttct agcgcgaccg gtgatggttt
     6841 tcgaaggtcc gcgcaggctt tcagaaaatc actgattaca ggttgtgatg cccatggatg
     6901 cgggagttct ggcggtggat cgggcttttt aaactgcaat cttgcccgtg cagctatctc
     6961 ctcgggcgtc ggtacgacat ccggctctgg ctgagtcatc cagaatacag tgcctgcgct
     7021 ggctgctact aaaaaaagta cggcaggcag tgtgaatttg ctgcgattgc cgacagtaag
     7081 tttgcaacgg cggagatctg ctgatgataa ccgggtaata atactttccc agttatcagg
     7141 atgttccaga ggagatacga cctgccattt ttcggggggc tcttcgttca tcgtcagaaa
     7201 aagactgacc ttttgagcaa catcagcatc atttcctgac agatcagcca taacggcagg
     7261 ctggccatta atggaggcaa ggaataaaag ctccttatcg ctgtaccgga agacgccgta
     7321 tccatttctc acccatgagc agaaagccag agcaagggaa aaataatgat tgcgctccgg
     7381 cctggccagt attcctgtgc cggtaatgcg gctgatcttc ccacgggaac ttctgctgcc
     7441 tacagtcact ttcagagagg taagccgtct ggattttaca tggggcttgt gtttctgatg
     7501 agaagggact cgctgttttt ttacgacagg actccattta agacaggctg cccacacgcg
     7561 atgatttact ttaggatctg cgagaagtac aaccgggttg atatcttcat cagccattgt
     7621 tgcctcctga cgggaaagtc ggggtaatga tgattacaag cgtgctgcgt tcatttttcc
     7681 cggtttgtga accaccaaat aatggatttg ctggcgtaaa cgtaccggct ttacttgtcg
     7741 tcgtattgtt ctgatcgaaa ccagtaacga caagtgattg cccttctttc agattgacct
     7801 tctggctcag tgaacgcagt ttggtatacg gcatttcgat gtaactgttt ccgtcttttg
     7861 acgtaaagct acggattgtt gggggatctg acagattaaa attcatctgc aactggagat
     7921 tgcccgtttt ctgaattaaa ggcagcaggg tcatattgaa tccggtggtg atcatgcccg
     7981 gcgttaatgt tgttgttgct cctacatccg tagtcgttgt tgttgctgac tgggcgacgt
     8041 aaaccgtctg atctgccatc tggataggta ccggcgtcag gtttgtgaca gtgctttctt
     8101 gtgaagtcac aacactgaca tcgccctgtt cactcagcgc tttaatcaga agactggaac
     8161 cgctgaattt ggcggcattc cctgttgccg tatccagaat tgatacgcct gcagatgtag
     8221 cgcctgtaaa atctccgctt gcattgttca acgttgctcc ggcggaatgt agcgatttat
     8281 aaacaaggtt ccagtccaga ccgaactgtt cgtttctggt attgctgacg ctcagaacct
     8341 gtacgttcag ggctacctgg cggttcataa tactgttttg ttcgtccaca tatcgtgcga
     8401 cggcttcctg gacagctgga gtatcagtga cagtcagcgt tgagctcgat gcagataacc
     8461 agtaacggcc tttttctggt gtcagcattg cttcaatagt tttccggatg tcttcataca
     8521 gatcgtattc ctgaccaacg gttgtgctct gagaggacgt tgcatcaccg gatgctgagt
     8581 tatcctggcc tcctgttgcc cccattgtgc ttgttgagcc agagctgaca ctggaactgc
     8641 tgctggtttt ggtgttcagc atatgaagtg gatacgttct ggtttcagtc agatagaata
     8701 caatccgacc attatccatg cgccagtaca gaccactccg gctggccatc agatccagaa
     8761 gaccattgat atctccctgc cacatgaggt tattcagcgt taatggctga gtggaggtag
     8821 tcattgttgt gctgcccagg ctgcttaacg gtagacgccc attttcatct ggtgctggta
     8881 gtgttcctgt catctggcgg gtagcgcctc cttcaagagt tgaattggct gcgtcaggcg
     8941 tgatgatcac agggatgccg catacggcag taatacgttg ccccagttcc tgcagagtga
     9001 tctctccttt tcttgcctgc gtgatgtagc aggccggagc tgtttgtttt ttctctcttg
     9061 atacctgagc gaccggaaca gggtttatcc attgattatc gagccaggta agagcctgcg
     9121 atttacgggc cgaaagggct gatacctttt cccgtgcatg tgctgagtct tcctgtgctt
     9181 ttttctgcat tttgttgatt tcgctgaaag tacatccgga gatggaaaga gcgacggcga
     9241 tcatgcaggg gagcaccgcc agcttcattg aacgctggtg tgattttttc atgaatatat
     9301 ccagattagt taacgtagac gacagaacca gcagtaatgc ctgccggggg tgtcagaccg
     9361 gttgcagttc ctgatagcca ggtgagttgc ccgttttgga agatccctat tagtgcagaa
     9421 ccacgacttt ttgaacgcaa agtttcaaca agacctggtt gttcaggcat ccatacccac
     9481 agacgcccct gttgtaactg atgtttaatt cgggagtccg gagtgacagg gagagccagt
     9541 ttatctgagg aaattactcc gtcctgctgg cctgatacgt atctgatatc attgattctg
     9601 ttagcaatca tcagtatctg acttgcccat acagacgcac ttgcatattg ctgggcgttt
     9661 gtggtttcag acatcttctg attctggtag ctaccagtga taatcagaat ggtcaggccg
     9721 acggccatga ccagccatcc cattagcgac catcccttgt atcagaaact gtaatgatgc
     9781 actgcatgcg agaagcctgt gcgtataaag gtttttgcgc agtccgatat aactcaaata
     9841 cctgctccat ggctgattca aatgatcccc ggaacattaa aggcgcatcc agacggtaat
     9901 cggttacaga aagtggccat atgaccatcc agtgcgttga tgccatgctt tcgcattttg
     9961 tttcttctgc ccacttgata atgttctctc ttaatgtggt gcctgccggc gcacgccact
    10021 ctttaccctg agataccggt ttaacaggcg ttccggtcat gagcgggata gacttgactg
    10081 tggagccggt tggagtcggg gtggcggcgg gggttgacga agatacgctg tttcctctga
    10141 atggatttcg tggtttgttc tggctatttg ctgttgttgg agattcaggg gaagtggatg
    10201 tggttaccat ggctgctggc gagatagtgg ttgccgtgtc ctgtgccggt gcagcagctg
    10261 atgttgtgac ggtcaacgtt ttattatccc agtccagatg gccccatagg tgttgttctt
    10321 ccagcaaccg attcagagaa cgggtccact gatcattggc agaccatgaa accagtttgg
    10381 tattcagctt gggcgtggct gcattctctt gtctgaattt ccatccttcc ggtagtaatg
    10441 tttttatcca ctgactgacg gtccggttac gggccgtcgg cgatagtgtt acaggggcgc
    10501 taccaggctt gcctgtaatg attaaccggg agaaattctg acttattgtt ggtgaaggta
    10561 acggtcggga ttcgctgact ggatgtgctg tttttactgt ctctggcacg ctgactctcg
    10621 gggggggagt tattttaggt ggagaaagac gttgttgcgt aagtgccaga acgccagcac
    10681 tctcactgat ttgtccgtca acaaaaacca gagcaggcgt atctgatggt gccacgttat
    10741 tgctgccgga aattccggca cagccggaca ataatgacgg cagtataagt gtcagatagt
    10801 tttttttcat aacggagact ccgtgttcgt atatacgtgt ttgttaattg gtgtgaggcg
    10861 gctggagagg acgaatgacg actgtcattc cgtccgaatg caaatgcggg agagaccact
    10921 gaaataagag atatacaaaa ggaattgata atctttcata cagacttgtt ttccgtgggc
    10981 atgtaataac agagaaattc aaaccataag attccagtct ctttataagt ttatttattc
    11041 ttgcaggagt aaagagatgt gaatgcataa caatggtttc atcattatta attgcttttg
    11101 atatcagaga aagtaacaca tcccagtgtt tcctcattga ctccgggcaa cgtgtatcgg
    11161 cataggggct aagatgtatg cttgattctt tactgccatt ttttttcgca aagaatgtga
    11221 agttgtattt agatacagac cttacgataa gaagcacgca tctgaaaagt agtggggaaa
    11281 agcagataat aaccactata cagaaatata atatataaga tagtgatatg tggtcagtgt
    11341 atagataacg gaagtattgc cacggggtag tgatgtacag gcaaaacgta caccaaagta
    11401 accatagata taatggtacg ttattatttt tcatgttagt tgatgccggc gactaagata
    11461 acagagtaag ggggattaac tgtttaattc tttggaacga aaaaatgcag ttcctgaaca
    11521 tcacacacca ttatttccac aggcttacca tttttatcca gtaaatttga attggtttca
    11581 aaagatttgc agttgttttt acttttaaat gaatcaaggt acagggtgtt aatgtcagat
    11641 tcgtcatctg ttgtttctct tgaaataata ctgttttcta tcaccacatt ttcatgtatc
    11701 tcatttggca ttttgtactg ttgcaaatat tgttggcggc ggatgcgtgc ctgttcactg
    11761 ctttctatga acaccaccgt gatgattgcc ccgtacatga taatcaggaa aacccagaaa
    11821 tcttttggta tcttccaaaa aagtgcggac agtttgttat ctgatgaaat taaaatcgga
    11881 aaaaggattg caaagacaat caaaatcgcg atggttaatt taaataaaaa cgacatggtc
    11941 ccctcctgtt ttgacgaggt cttttgtttt tgttgttttt acgacagcca gtcctgcagg
    12001 tgctgatggc gggagcggct acccttgcgc cagcggcagt ggctaccggg tgcacgttcc
    12061 aggcggaaca gaaatcggtt gttgcatagt accaccacaa ggcagttatc cggcgtgcag
    12121 aagcgtcggg caagctcatc gggatctgtc tgtgttttcg ttgaaaacag cttgcgtttg
    12181 cagtgcgtgt cgacgaccag tacctgtagt cgtccgggat gttgctgtgg catcaaagac
    12241 tcctttcgga acgggaaaag aagcagctgt accgtcgtaa ctgcacgtac cggttgcgaa
    12301 aaaacggcac aagcgagtag tgacagaaag aatcccggga acggcagaaa gggtggtaca
    12361 ggctgcgaag acgggtcacg gggtgtgtcg ggaattaacg cacgtgaggc gtaatgagag
    12421 cgaagctctt tttgccaggg tcatctacgt tgattttcat gtatgttttc ctgtcggaaa
    12481 tcgtggtgct cttataactg ttattccctg tttccagaat cgtttttcag ggtcagaatg
    12541 acaataccac agcggggatt tatgtgaggc actttccact gagataagat tctgctgacg
    12601 atgaggatgc ggaacatgac gctattcatg gcggattccc ttcgttccgc tgttacatcc
    12661 agcccttttc ggcgcagaga gcaaatcagc ttttgtgtcc tggcttcagt cagaagatgg
    12721 gatttcatgt agatacgttt cttctggcgt aataaggggg gcagcttttc gagtaattgc
    12781 ttgaagtgtt tttttatcgc cttctttgag gaagtatctg cgagtgcgct caggaaaaca
    12841 catactgtgt gattgtcaga catgtctttg gttttaaaac tatttttcct aaatattatt
    12901 ctaagcagaa tgaaaaacgg acggactaac agcgggagag ctatcatccc caatatctct
    12961 actagcgtcc atatgttcat ctggtacaag ccatccttgc agagcaaaat cgctataatg
    13021 caccagacgc tatatatagc taatggaata cgatattgga gtttcatgta ttgctcttcc
    13081 tcagtgtaat gtgatagtcg tctgctcctg tgttgcaccc cagacatctt ctggcaggct
    13141 gggcagcccg ataaataagg tgtgaaagag ggtttctctg cgttcgatcc cgaattgatg
    13201 ttcgtatcct ctcaccacct gaaatccttc gtcggagaat attaactctg ccgcttccag
    13261 aagctgcata gttttgcggt gtagtggagt gatcagctgc aacgtaactg gtgccctcat
    13321 atcgcctgtc ggtagaaaac actcttcggt gcgacttagt tgtagcagat gacgtttccc
    13381 ggaacaggcc cgttcaatgg attcattgaa caccctctca ggccatgggc agacatatgt
    13441 gcgccagcct gctttctgta atttacccag tgcagcccga aaacgaaaca acggacattc
    13501 cccgacagtg gcagagctga gaaagagcgt attcagcgtt ccgatgatgg gaatattgcg
    13561 atctttttcg gtcaatgctt ttaacgccat gatttcgcca gggagactac ggaactcagg
    13621 atgtctgctg gcaccagaaa gccattcaag caggtaatgc cgtaatccgg tttcactggt
    13681 tatattgtgg ttttcctgtg ccgttttcag ggcattcagt gcacaccaga gcaactggcg
    13741 cacgggatgt tctgtcgtgg tcatgcgaat gtcctactgt gagatgaaaa gaagaccggt
    13801 atccgttctc tccgccacac gtgatggcac gggccggcct ttgataaaac gaatcaatac
    13861 tctggggtga agccttttat ttctttcgcc attaagaaat acacggttcc atcaaaagac
    13921 agggggagtc catggtgggg atggcttcgt gcaggcttca ctccggaata ttctcttgaa
    13981 ttgaaaaaag cccggcccga aggccgggaa atactggcta tgggatgttt acaaggcgct
    14041 ccataaagcc gtgatgttcg gcatttgata ccagttccag gagcagggat atcctcgact
    14101 caggctggag agtattttcc attctgagga gatactgata ttgtgctttt gtcagccata
    14161 tgctggaagc cgcattcagc tcttccctgg ctcccggatt tagcacaggg tccgggtaaa
    14221 tcttcattaa tccatcaaca atatctttat tgacgggttt tatttcaccg ccaaacttca
    14281 cgaaatcaat gaaggcgcta tgtcgacgta atgctgatac tgggtgaatg tcaaaattag
    14341 ccagaataaa aaagtaccct ggaaaaatag cgaatattct ttcacggtaa cagcacctgg
    14401 agtctgcccg tcttatcttt cttgttatta atggagtcca tggtacgacg ttttgctcat
    14461 tcagccatga gaataaagat tctctgtttt tgccggctgt attatattgc gcaagatacc
    14521 agttccggtt attcaggtgt tctatattca cgaagttcaa cgctccgcgc aaaaccgatc
    14581 catgatggac gattttgcga tgtcaggcga atatgctaaa tcatatgtct gaagcagtgt
    14641 ccagcccata tctggataaa ctgcagttct gacaggcata taaccagcag actctagtac
    14701 gttctgtatc ggtgatttgg cgaactcaca gataaagaac ggctaagcac taatatcagc
    14761 aatacagtgg cacagtattg tgatacgcga aataaaacat cagcacgtca gttcagagta
    14821 aggtcgccat atatcgggca tgagccagat acaaataact gggacaccgt ctgaagtgat
    14881 ttgctttatt aaagtaactt gtgcttggtg tttatgttta tcaatttggg atcgcctgta
    14941 aagtgcaaaa tgcaatttcc atgaaagtgt cccctttaat ggggacacct gttcacattt
    15001 tttcgttttc tttagctgta ttgctaatca tgaaaacgaa agttcgcatc agcacagcag
    15061 agcgaagtgc atcatatcct tccagattta aattcttcag gcttcgcttc aggtaggttc
    15121 gtaccgtttc gggagaaagc cgacagttct gagcaatggc gtcgtaagga acgcccattg
    15181 cgtaaaaaac gcagacttta agctggtttg ttgatagctc gggaaacatg ccttccaaca
    15241 gagtgatggc cgggagtttc tgtatcagtt gaggtgacat agcaaaggtc tccgatgaca
    15301 actaaaaacg ctatcaccga gttcctacgc tcattggtga tagcccagac gggggtagga
    15361 ataccggcgt catcggaaac cggccaggct aatgcctgcc ccgcctgagc taccatagat
    15421 gacgacaaga aatacaagtt cctgtcttta tggtatggca aaaaacgacg ccaaatgaag
    15481 aaattaactt ctgcgccgac gatgactacg cgggttccta cgcccgatca cagttttgcc
    15541 tgtgaccagt aaagggtact tagtataacg caccgagtca actaacagct taaaaaacat
    15601 acaaaaaagc gtcaaaactt aacgctccgc gccgaagcga tccaggcatg atgcttctgc
    15661 tttgtggcgt tcgaacatgc tggtccatat gcacgaaacg gtgtctgacc ctcgtccaga
    15721 caatccgcag ttctgacgaa catatgaaca aatatgctct tgtagaggct gttgttacac
    15781 aattggcggg ttgtgtaaca aatggtttta tcatctaaga attattctat ttacgatcga
    15841 attatctaca aaatgatttt ttaaaggtta tttagatcgt tta
//
