LOCUS       NZ_CP070004             2101 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1300 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP070004
VERSION     NZ_CP070004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357442
            Assembly: GCF_016903915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1255.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,439
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 5,075
            CDSs (with protein)               :: 5,075
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 94 of 247
            Pseudo Genes (incomplete)         :: 157 of 247
            Pseudo Genes (internal stop)      :: 46 of 247
            Pseudo Genes (multiple problems)  :: 48 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1300"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1300"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(2081..2101,1..945)
                     /locus_tag="I6K30_RS26815"
                     /old_locus_tag="I6K30_26820"
     CDS             join(2081..2101,1..945)
                     /locus_tag="I6K30_RS26815"
                     /old_locus_tag="I6K30_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            949..1215
                     /locus_tag="I6K30_RS26820"
                     /old_locus_tag="I6K30_26825"
     CDS             949..1215
                     /locus_tag="I6K30_RS26820"
                     /old_locus_tag="I6K30_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          374..605
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcggactaca gcccccgtga tgcagtttgg gatacccagc gcacgcttac cgattctgtc
       61 gggggtatct accagactgc tgctgaattc gagcgctatg cactccgtat ggcctcctgt
      121 agcggtttgt tacgttttgg ttggtctacc atcatggaaa ccggagaaac gcgcctacgg
      181 cttcgtagtg cgcaattttg ccgtgtccgt cattgccctg tctgccagtg gagaagaacc
      241 ctcatgtggc aagcccgttt ttatcaggct ctaccgaaaa tcgttgtgga ttacccgtct
      301 tcccgatggt tgtttctgac gttaactgtc aggaactgcg agataggtga acttggaaca
      361 gtccttacag caatgaatgc ggcgtttaag cgaatggaaa agcgaaagga gctatcacct
      421 gttcaggggt ggatcagggc tacggaggtg acgcgaggta aggatggcag cgcacatccg
      481 cattttcact gtctgctgat ggtgcaacct tcttggttta aagggaagaa ctacgttaag
      541 cacgaacgtt gggtagaact ctggcgcgat tgcttgcggg tgaactatga gccgaatatc
      601 gatattcggg cagtaaaaac taagacaggt gaggttgtgg ccaacgttgc cgagcaactg
      661 caaagcgcgg ttgctgaaac gctgaaatac tccgttaaac cggaagatat ggcaaacgat
      721 cctgagtggt ttcttgagct gacgcggcag cttcacaagc gccgttttat ctcgaccggt
      781 ggggcgctaa aaaacgtcct ccagttggat cgagaaacca atgaggatct tgtcattgcc
      841 gacgatgtag gggatggcac tgatgacggg aagcggacgg cgtttgtctg ggattcaggt
      901 aaacggcgtt acaaacgcgc ccctgagaag gataaatcgg attaacgtat gaatattaat
      961 attgaatacc tgaatggaaa taagactatt ggtttatttt ttttaagaag tgaagcggtg
     1021 attcctgaca ggtttaaaaa ccttattttg cttattgatg gattaagttt tggcacattt
     1081 ggttttcatc cgcacgaagg ttttgaggat gaattaattt tatatattca gaaaacaaac
     1141 gagagggtaa aaactctttt tgtgaaaata gatttaaacg gtatcaagtt tggattttta
     1201 agaacgcatt cttagttctg gaaaagagcc agcggcaggc tgaggtgata ggtacgagat
     1261 tgcatgcaat ctctagtgct ctgtctatcc tgcattatcc tcagcattat cctcagcatt
     1321 atcctcagcc ttgccaactc gacaccaatg caggatagac aatccgatgt caaatgttaa
     1381 cactctgcga gtggtacatt ttccccggat tatcgtcctg agcctgccgc tggctctctt
     1441 tctaccgcct cgctttgctc gttgctcaac gcctcacaga cacggattaa aatccgcatc
     1501 cgttcaccgt tttttaaagt ccgttaaaag catgatgcca tctccgagag ttaatctcgt
     1561 caaatgctaa atcgtggggg tcccctttgg ggttccgatt tagtgattga cgacaccacc
     1621 gattaaaaaa cttatgcggg gtggatggtt tcacgaagtg aggccatcca cctgtaagac
     1681 agggttttgt ttttattccc tgttttggtg atcgggtgtg tggaaaaggt tggggtaagc
     1741 cgttcggggg tgcttgtttt ggggggttaa aattgtggtt attttttgcg caattctcgc
     1801 gcgtgatcct tgtatttata cttaagggat aaatggcgga tatgaaatag tggtttagcc
     1861 cagtaatgac gaggctttga gtgggttttg acaggtcaaa gaaaatggag cagaattgag
     1921 gcgtttttaa tcggcgttgg ggagtgcgtc aacactcccc aacatttcga atgtgtcacc
     1981 tcagcggcaa actctggtga catgtactgg ctcgcaatgc acaggtacgt gatgaatata
     2041 ccacatcaaa tcacagcctg cccagatcgg agcaggctta atgtcagaag ataaattcct
     2101 t
//
