LOCUS       NZ_CP070001             5164 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1300 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP070001
VERSION     NZ_CP070001.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357442
            Assembly: GCF_016903915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1255.12x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,439
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 5,075
            CDSs (with protein)               :: 5,075
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 94 of 247
            Pseudo Genes (incomplete)         :: 157 of 247
            Pseudo Genes (internal stop)      :: 46 of 247
            Pseudo Genes (multiple problems)  :: 48 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1300"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1300"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(4822..5164,1..56))
                     /locus_tag="I6K30_RS26745"
                     /old_locus_tag="I6K30_26750"
     CDS             complement(join(4822..5164,1..56))
                     /locus_tag="I6K30_RS26745"
                     /old_locus_tag="I6K30_26750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            233..1159
                     /locus_tag="I6K30_RS26750"
                     /old_locus_tag="I6K30_26755"
     CDS             233..1159
                     /locus_tag="I6K30_RS26750"
                     /old_locus_tag="I6K30_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1162..1695
                     /locus_tag="I6K30_RS26755"
                     /old_locus_tag="I6K30_26760"
     CDS             1162..1695
                     /locus_tag="I6K30_RS26755"
                     /old_locus_tag="I6K30_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1761..1970)
                     /locus_tag="I6K30_RS26760"
                     /old_locus_tag="I6K30_26765"
     CDS             complement(1761..1970)
                     /locus_tag="I6K30_RS26760"
                     /old_locus_tag="I6K30_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            3704..4618
                     /locus_tag="I6K30_RS26765"
                     /old_locus_tag="I6K30_26770"
     CDS             3704..4618
                     /locus_tag="I6K30_RS26765"
                     /old_locus_tag="I6K30_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          3705..4618
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          234..1156
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcttcctga tgaatccgat ccgtttcttc agccattcga tgttgctgat gcgcatgttc
       61 gtgatgctcc ctggtgtttt tttgcggtgt gttgtggtcg tgcagtatat cgcttgcggt
      121 cggcgggtgc agggggtatc ttccgtgcta attcgtaaaa ttagaaggaa gcgcacttat
      181 atgtttctct ccttcgtcga gaaacagtgc gctcctgcag agggctgaaa ccgtggcaac
      241 ctatcacctc agcgttaaat ttggtggcaa ggggcaggct gcaaaccacg ccgattacat
      301 cgagcgcaaa gagaaatacc gcgaccgtca ggatttggaa tattccgcac acggaaacat
      361 gccggaatgg gccagggata atccttccca tttctggcag gcggctgacc agtttgaacg
      421 tgcgaacggt tcgacctata gggaacttga aattgcatta ccccgtgaac tgacaccgga
      481 acagcgcctt gaactggtgc aggattttgt acgtcaggaa gctggcgaac ggcacgcctg
      541 gagttttgcc attcacaatc cgaaagcgag cattgacggc ggcgaacagc ctcatgcgca
      601 tatcatgatg agtcagcgtg tgaatgatgg cattgaacgt acaccggaac agtatttccg
      661 gcgctataac gccaggtatc ccgagcgggg aggtgcgaaa aaggattccg gaagcctgac
      721 tctcactcag caaaaagagc agttgcggga attgcgaaaa cgctgggagg tgaagcacaa
      781 cgaacatatg agaaagcatg gctttgaaag gggcttcatt gactgccgga cgctgaaaga
      841 acagggcatt gaacgacgtc ccgaagtgca tctgggtttt gaagctgccg gacggcttga
      901 tgatgcccag cgtcatgaca tcatgcaaaa acggtctgaa ccggactatt cccgccatct
      961 tgaacaggcg cgaacggtcg ggcatgacat cagggaatgg ctggacactc cggttaagca
     1021 acatgaactg gtcagaaggc tgcaacatga tgttgcgcgg gagcatggct ttgacatgag
     1081 agacagagtg agcagacaga accgaactgc acctgaacgg gttaaatcac cgtcacgtgg
     1141 attttctatg ggattctgaa catggccagc acagaagaaa aattactcac gctcctgtta
     1201 tcggtggagc atctgcaaca taccgcaatg gagcaacaga aagctgttgc tgattctctg
     1261 gctgtggcaa gggaaactct tcgcggatat cgggatattt ctgacggtgt ggcaaaacag
     1321 gtcagggatg aagtcagaaa tgaggtgcag aagctggacg tggcaggact gataggtgag
     1381 cgcatcagcc aggattttca gcggctggaa gagtcagtcg caaacatgaa ggcaaacgcg
     1441 gatgccctga acagggatat tgcgacaatt cacagcaata tcgtgcagga atacaacacc
     1501 cttaaagggt atagctggaa atggctgggt ggggtgtcag tggcttttct ggttatgttc
     1561 tgtgtgctcg gatggggaat gaaaacaata ctcgacagga actacaacgc cacagcggcg
     1621 gtgtatcaga aagttgtgac actggaagat aaactggtgg agaaacctgt cacggggaag
     1681 agccggaaaa actgacgcgc ttcgcttgtc caacgcctca cgacccgcga aaggatttcg
     1741 cgaaccgttc accgtttttt tcaatatgat tctgtctgct ttggtcgata aagcagacga
     1801 tagtattcca gccttcgtat atataacggt acgtgctcat cagtaagagc tggcggtatt
     1861 gtgttatcag ggggaagttt tttcagatac tcacgaaatg cctggcttga tgccataaaa
     1921 tctacagtag catcgataat atgtggggtg ttttcaccta attttgccat gatgataatc
     1981 cttatattat cacggtttgt gaatgatagg gtgtcactat tatttatgcg ttagtgaacc
     2041 agaaaaaatc ccccgttggc atagggggat atgtaaggag aaataacttc gcaggtgttc
     2101 ttgtttgtat aacgtgaaaa gactgcacgc aacttaataa gtctgccgta cttactttca
     2161 gttttcaaca taaggtttat aacaatgtat ggaatggaag tgagctttaa ggtttcagct
     2221 ctaaaaaata aaatatttat tagttggctg gttgttgttc ttgttctgta tgtatataaa
     2281 cgctatcgac atattatgac cgggaacatt ccttaccccc tctgcgctcg cagaggtttt
     2341 ttttgtcatt aattaataat gaactgaaat gcagttccga tacctccaat tgcacattat
     2401 ccctgacata tggtaccgga accgcattaa ttttatgtta taagagttta tttttaatgt
     2461 ccagatggtt taatagtatt tatatgtaat tagctgtatg gatagtactg gttttattgt
     2521 ataacttgtc gttttttttt attgtttttg tgtggtgtgc taatctttaa gttataaaaa
     2581 aaccccttgg aatacagggg caaaagtaag gaaaacaaat ggatgctcag tttgtgtgac
     2641 actttaaact aatggaacta tagagtggag gttgttacag tcgcttttat aaaacagcaa
     2701 ccagtttttt tcaatagtct gatgatggtt gtgacagttt tgtgatattg ttcaaaatgt
     2761 ttcttgttga atgtttgttt tttggtctat tggtgtctgg ttttattttt tctgctatta
     2821 catatcaaga aatgtgattg gtgtagcaag agtggaatgc gatgtggata aactaattgg
     2881 ttgtcgaatc taatttactt agaaaactac acttgttttt gtgtatgaat ttagtgaatt
     2941 tgtctgtcta tgtgttttcg tgcatttata aatccttcgt caggatctta aatttagtgt
     3001 tgaatatgta gggcctgtac ggtaattatt atttagcgta gctacttcat cgttgttcga
     3061 acacaaccaa caacctgtaa ttgttgtttc tcttggttcc ccacttttgt ggggtttttt
     3121 atctcttccg tgcatcgtaa agcgcggact ccttctggaa aggttgtttt agggcatcac
     3181 tgaggcattt tttgcgtttg tgaatattga gcatccgata actaaaatat ttcccataga
     3241 ctctgattct tgccaccgac cccaggtcgg ttttttttgt cctggcgaga tgtgaagttt
     3301 ggaaacagga aaccccgtca gagcgccacg caccagggga gaaaacttgt cgttttgagc
     3361 cgttcggtgt tcagaacgca cgaaaccgat cgcacatcgc tttcgtgcat agttatgcaa
     3421 cgccctgaaa acgattctga cgcgtttttc tggtttggcc tggtgttttc ttgtcttttt
     3481 gcgttttttg cgccagaaag cgattgatgg cgttttacat ggtacgtaca acgggagtta
     3541 tggtaaatgg atcggatttg cgggaaggct cgacaggttt tgtcgttggg ggtagtgtaa
     3601 gcgactgaaa aacaaacgcc cctgaaattc gtgtctatcc gccaagactt tcacgaaaat
     3661 acaggggcga tgtcggtcga taacctggaa ggaatcgtaa cacatgagcg ccgcgcttca
     3721 atacttcgaa gaaaatttac cccaccgtcc ctatcacacg gatgatctcg ctttcggcct
     3781 tcgtatctcc ggcaaagggc gcgcgcttct tgcacggtat atccagcaga accagcctca
     3841 tgcacagttc tggctggttt ttgacgttga ccgcgagggg gctgcgattg actggagcga
     3901 ccggaacgct cccgcgccga acatcaccgt taaaaatcct gtgaacggac acgctcacct
     3961 gctctacgca ctcaacatcg ccgtgagaac cgcgcctgat gcctcggtta aggcgctgaa
     4021 atatgccgca gcggttgaac gttcgctgtg tgagaaactt tgtgcggatg tgaactacag
     4081 cggcctgatt tgcaaaaatc cgttccacct ggaatggctg gtgatggagt ggcgcgagga
     4141 agcctatacc ctcgatgaac tggctgatta tctcgatttg agcgcctcag agcgtcgtag
     4201 catcgataaa cattacggga tggggcgaaa ctgccacctg ttcgaaatga cgcgcaaatg
     4261 ggcttacagg gcgattcgtc agggctggcc agcgttctca cagtggcttg aagcggtgat
     4321 ccagcgtgtc gaaatgtaca atgcatcgct tcccgttccg ctttcactgg ctgaatgtcg
     4381 ggctattggc aagagcattg cgaaatacac gcacaggaac ttcacgccgg aaactttcgc
     4441 acagtatgtg gctgatacgc acacgccaga aattcaggct gcacgcggtc gcaagggcgg
     4501 ttctaagtct aagcgcagca cagtagctac atctgcacgc acgctgaaac cgtgggaggc
     4561 attagggatc agtcgcgcct ggtattacca actgaaaaaa cgaggtctcg tagagtagac
     4621 caaataagcc tatatcagat aacagcgcct ttctggcgtc tttttgagca gcaggttttt
     4681 tgccgttttc ccccgttcag ccctgcaatg gcgcggcttt ccgcgtgatt gtggtggtag
     4741 tgctcgccgc agtctcatga ccgagcgtag cgagcgaatg agcgaggaag caccagggaa
     4801 tcggaacacg gatggaggca cttacgcgtc tgggtttggg gatgtctcca gatcgtactt
     4861 aagttcctta agtgcttgta atatcctgtc ttttttatag catagctcgc ctgtttttgt
     4921 gtctaccagt cctgcttcga tcagtagccc agccgcgaga aagagccggt gattgcgttc
     4981 ccgtcgttgt ttttttccct cgattcgttt ttggaccgcc tgttttcgtt cgctctcctg
     5041 tgcctgtaag tcgttttttt ccgctgttgc cagtacgtgg agaagtttgc gttcctgttc
     5101 tgtgagtccg gcttcgttgt cgatcaggtc aataatctgt ctctgtcgtg cggtctgttc
     5161 tccg
//
