LOCUS       NZ_CP069988             3909 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1291 plasmid unnamed8.
ACCESSION   NZ_CP069988
VERSION     NZ_CP069988.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357433
            Assembly: GCF_016903855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1725.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:14:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 53 of 214
            Pseudo Genes (incomplete)         :: 163 of 214
            Pseudo Genes (internal stop)      :: 38 of 214
            Pseudo Genes (multiple problems)  :: 38 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069988.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1291"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1291"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..1187)
                     /locus_tag="I6K21_RS25335"
                     /old_locus_tag="I6K21_25345"
     CDS             complement(<1..1187)
                     /locus_tag="I6K21_RS25335"
                     /old_locus_tag="I6K21_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_204652622.1"
                     /translation="MFVCLTIENFRSVQENFTLDLSAFGSNSHLVNHIYKNAEMSVGTL
                     MSAGIYGANASGKSNVLMAFEAIKYIATDSGDLKEGTRIPCYEPYALSLATNESPVRFV
                     ADFYSVEGNRFNYEVKYIKDRIIFESLDYYPSRVKANLFTRDEGDTWETIKFGGHYRGG
                     VKKIPFFPNNSYLAKAGNNAASPDIIKEAYDFFRKGIRHIGLNEKIRISSFGKREEVVS
                     ETAEVLCMVDTGVAGISVREVESDLPIRMDDDIPPEIKEMIEEDYKYRYLFSHATEEGG
                     SVSFPMSRESEGTQKLFEIIPLIRSAFNNSMVVIIDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLIFSTHNMQLMTPEKMRRDQIWFCEKNKGASALYSLDDFDKKKVKTTTP"
                     /name="ATP-binding protein"
     gene            complement(1535..1858)
                     /gene="mobA"
                     /locus_tag="I6K21_RS25340"
                     /old_locus_tag="I6K21_25350"
     CDS             complement(1535..1858)
                     /gene="mobA"
                     /locus_tag="I6K21_RS25340"
                     /old_locus_tag="I6K21_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2130..2324
                     /locus_tag="I6K21_RS25345"
                     /old_locus_tag="I6K21_25355"
     CDS             2130..2324
                     /locus_tag="I6K21_RS25345"
                     /old_locus_tag="I6K21_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2811..2981
                     /locus_tag="I6K21_RS25770"
     CDS             2811..2981
                     /locus_tag="I6K21_RS25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059287940.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGNRIRRALMC
                     NQYYRVLEVMA"
                     /name="hypothetical protein"
     gene            complement(3165..>3909)
                     /locus_tag="I6K21_RS25350"
                     /old_locus_tag="I6K21_25360"
     CDS             complement(3165..>3909)
                     /locus_tag="I6K21_RS25350"
                     /old_locus_tag="I6K21_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189381.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_204652623.1"
                     /translation="NCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPYDAIRDFTMEYPT
                     HIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFFQKNQIGKLVDLV
                     MPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETINIL
                     YEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYTDSEKEEIQQSIL
                     KQIEKYSDFPYVVNYYRNRLSALFD"
                     /name="type II restriction endonuclease"
     Typing          complement(1868..2093)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.9e-75"
     Typing          2354..2883
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.786"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1539..1858)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.35e-60"
ORIGIN
        1 taaggtgttg ttgtttttac ttttttctta tcaaaatcat ctaaactgta taatgccgaa
       61 gcacctttgt ttttttcgca aaaccatatc tgatcccttc tcattttttc aggagtcatc
      121 aactgcatat tatgcgtaga gaatattagc tgagatccct ttttgttaac atcaggatca
      181 ttaaatagct taacaattag atctgcaata tgtggatgca aactattatc tagttcatcg
      241 ataatcacaa ccatgctgtt attaaatgca cttctaatta aaggtattat ttcaaataac
      301 ttctgagtgc cttcagactc tctggacata ggaaaagaaa cactcccgcc ctcctcagtt
      361 gcatgagaaa acaaatatcg atacttataa tcctcctcta tcatttcttt tatttctgga
      421 ggaatatcat catccattct tattggtaaa tcagattcta cttctcgcac ggatattcca
      481 gcaacacctg tatcaaccat gcacagaacc tcagcggttt cacttactac ttcctctctt
      541 ttaccaaaag atgagattct tattttttca ttaagaccta tatgccttat ccctttccta
      601 aaaaagtcat aagcttcttt aattatatct ggagatgccg cattattacc ggccttggcc
      661 aaatatgagt tattagggaa gaatggtatt tttttcaccc cacctctata atgaccacca
      721 aacttaattg tttcccatgt gtcgccttca tccctagtaa acaaattagc ctttacccta
      781 gacggatagt aatccaatga ctcgaatata attcgatctt taatatattt aacctcataa
      841 ttaaaacgat tcccctcaac agagtaaaaa tcagccacaa accgaactgg tgactcattc
      901 gtagccaacg agagggcata cggttcgtag catggaattc gagttccttc cttcagatcc
      961 ccgctatctg tagcaatata tttaatagcc tcaaaagcca tgagaacgtt ggacttacct
     1021 gacgcattcg ccccataaat cccagcactc ataagtgttc caacagacat ctcggcgttt
     1081 ttataaatat gattaactaa atggctattt gagccaaacg cacttaaatc aagcgtaaag
     1141 ttttcctgta cggatctaaa attttcaata gtcaaacaaa caaacatagg caactcctaa
     1201 atgagttttt tcgccaaaaa agcgaaaaaa aagcaataaa caagatacag cacatgcttt
     1261 aaatttgaaa caacatcatc ataaagaatc ttgtacgacc attcaccaca caacatttaa
     1321 cataacctat aaattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
     1381 cgcaaaaaga gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
     1441 tcgctgcata gctatgcatg aaagcgaatg gtgatcactt tgggagctta cggtgttcat
     1501 accgtctgtt ttcgacagtt ttctctccgg gaagctaatc tgccataagc ctggataaca
     1561 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgacac
     1621 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagta
     1681 cccgatcatc agtcagtgag ttaactttct taccgagagc tgccgcccgt aaaaaaccgg
     1741 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1801 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1861 aacatggctc cgtatctgac atagggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1921 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtctgc aagttcgaat
     1981 taccaacaaa gcactgctgt tttttggcag tgacagtgtt gtctgactga aatatccgtc
     2041 ggcagaatac cggcggtaag acggtcgaaa agtggtgcgc gatataatca cacgacgcag
     2101 atgacagaca gaacggagaa aacagaaaga tgaacaagca gcagcaaact gtactcaaca
     2161 tggcgggatt cataaaaagc cagagcctaa cactgctcga aaagctggac gcactcgatg
     2221 ccgacgaaca ggccgccatg tgtgagaagc tgcacgaact cgcggaagaa ctccagaaca
     2281 gcatacagac acgctttgaa gtagaaaata gcacagagat ataacgctca gggcaggatg
     2341 ccgaacggca caacagagcg taaggagaaa atgtcacata aggcgctctt ccgcttcctc
     2401 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggtgtcag ctcactcaaa
     2461 ggcggtaata cggttatcca cagaatcagg ggataaagcc ggaaagaaca tgtgagcaaa
     2521 aagcaaagca ccggaagaag ccagcaccgc aggcgttttt ccataggctc cgcccccctg
     2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2761 gctgtaggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2881 taagacacga cttaacgcca ctggcagcag ccactggtaa ccggattcgc agagcattga
     2941 tgtgtaatca gtactacaga gttcttgaag tgatggccta actacggcta cactggaagg
     3001 acagtatttg gtatctgtgc tctgctgaag ccagattacc tcggttagat aatctgccag
     3061 gattagctaa ccttcgaaaa aaccacctgc cagggtggtt ttttcgttta caggtccaag
     3121 attacgcgca gaaaaaaagg atctcaagag atccttttag ttttttagtc aaaaagagca
     3181 ctaagcctat ttctataata attaacaaca taagggaaat cactatattt ttctatttgt
     3241 tttaatattg actgttgaat ttcctctttt tcagagtctg tgtaacttac attattccac
     3301 tttcttgata attccattgc tgactgtagc atatcttcaa aggttagcac cctattatta
     3361 tttttatatt tgaaattttt attttctatc gtcgttacaa cgaccacatt agcctcgtac
     3421 aatatattta ttgtctcctc gctaaaagaa tcatctagtg tagctagata catttctcta
     3481 attcctgttc tattaacctc ttcaggtact tcttgccatc gctccctcag tgttgtttta
     3541 gcggaaatca acattgtatt tcttttgtta gatgtgtact ggactacacc tggcataaca
     3601 agatctacaa gtttacctat ttggtttttt tgaaaaaatg acttcccaat agccccctgc
     3661 acatcaacag gaattcctgc ccccatcatt aatagctcaa gaatagactc aaactctttt
     3721 cctgccctgc tacgtctgga ttgagtgtta gacagtgcca aatcatatat gtgtgtaggg
     3781 tattccatcg taaaatcacg aatggcatca taaggactca tatcttttat tctttcttca
     3841 tcaataagat atgataacat tcttgttgtg aattcttttt caaatggttc aaattctttc
     3901 caacagttt
//
