LOCUS       NZ_CP069987             1464 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1291 plasmid unnamed7.
ACCESSION   NZ_CP069987
VERSION     NZ_CP069987.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357433
            Assembly: GCF_016903855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1725.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:14:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 53 of 214
            Pseudo Genes (incomplete)         :: 163 of 214
            Pseudo Genes (internal stop)      :: 38 of 214
            Pseudo Genes (multiple problems)  :: 38 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069987.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1291"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1291"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(1114..1449)
                     /locus_tag="I6K21_RS25330"
                     /old_locus_tag="I6K21_25340"
     CDS             complement(1114..1449)
                     /locus_tag="I6K21_RS25330"
                     /old_locus_tag="I6K21_25340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_273683913.1"
                     /translation="MTLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(6..87)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(544..731)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
ORIGIN
        1 tttggggtgt cggggcgaag ccctgaccca gtcacgtagc gatagcggag tgtatactgg
       61 cttaaccatt cggcatcagt gcggattgta tgaaaagtgc gccatgtccg gtgtgaaatg
      121 ccgcacagat gcgtaaggag aaaatgccag ttcaggcgct cttccgcttc ctcgctcact
      181 gactcgctac gctcggtcgt tcggctgcgg cgagcggact ctcctttctc aaaaacgcta
      241 accctgttat ccacagaacc cggggataaa ccaggaaaga acatgtgcgc aaaaaacgaa
      301 accccgggaa aggccgcgcc ggaggcgctt tttccatagg ctccgccccc ctgacggact
      361 tcacaaaaca gacacttttt cggtgcatct gtgggagcct tgcggctcaa ccatgaaatc
      421 cgccagcggt ggcgaaaccc gacgtggctt aaagatcatc accgtttccg gtgaatcgct
      481 ccctcctgcg cccccacgtt cccgccatat cgattaccgg ataccttttc cgcctttctc
      541 ccttacggga cacgtggcgc tttctcatag ctcacgtctt ctggtatctc ggggcgactt
      601 aggcagttcg ctccgggctg ggctgtatgc aggaaccccc cgttcagccc gactgctgcg
      661 ccttctccgg taactgttct cttgggtcca acccggaaaa gacaccacaa agcgccactg
      721 gcagcagcca ttggtaaaag tcatgtgcga gatatttaga gagtacttga agtggctgcc
      781 ggagtgcggc tacactagaa gaacaatttt ggtgactcgt ctcgcataag acagttacct
      841 ggttaagcag tctgcaagct gacttaacct tcgatcaaac cgccctgcaa ggcggttttt
      901 tcgtttacag gcagcagatt acgcgcagaa aaaaaggatc tcaagaggat cctttgatct
      961 ttttgttaca aacgtctcta tttgactccc ctgagagcgt tttagcgcgc ctctgacccc
     1021 cttggttaca ttccgacacc agaaacgctc ccgtttttta cgtgcatttg gcacggtatc
     1081 gatggaggcc ggggggcaat cctgaacaga atgttactcc tctgagtatt ttctgttaag
     1141 aaaatataca cttagcacag ttacaataaa tcctaccccc tgtaagaatg tgaattgttc
     1201 atccaaaaac aaccatgaca aaaataatgc aactagcgga ataaagtaca acattatccc
     1261 tgccaaatga actgggagcc aacgcaagga gttcaagtaa aaacaaaaag ctaaaaaata
     1321 ttgtaatatt ccagatgaaa aaacaataat cacggattcc aacggaagat aaattgatga
     1381 tttagatata aaaataaaga gcgatgacag tataagggag aatgtcaact gtccgctcaa
     1441 taatgtgata ggctcaaacg attt
//
