LOCUS       NZ_CP069985             4072 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1291 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP069985
VERSION     NZ_CP069985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357433
            Assembly: GCF_016903855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1725.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:14:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 53 of 214
            Pseudo Genes (incomplete)         :: 163 of 214
            Pseudo Genes (internal stop)      :: 38 of 214
            Pseudo Genes (multiple problems)  :: 38 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1291"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1291"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(3975..4072,1..2050)
                     /gene="mobQ"
                     /locus_tag="I6K21_RS25300"
                     /old_locus_tag="I6K21_25310"
     CDS             join(3975..4072,1..2050)
                     /gene="mobQ"
                     /locus_tag="I6K21_RS25300"
                     /old_locus_tag="I6K21_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_032495499.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2308..3009)
                     /locus_tag="I6K21_RS25305"
                     /old_locus_tag="I6K21_25315"
     CDS             complement(2308..3009)
                     /locus_tag="I6K21_RS25305"
                     /old_locus_tag="I6K21_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3542..3796)
                     /gene="mobC"
                     /locus_tag="I6K21_RS25310"
                     /old_locus_tag="I6K21_25320"
     CDS             complement(3542..3796)
                     /gene="mobC"
                     /locus_tag="I6K21_RS25310"
                     /old_locus_tag="I6K21_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..261
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.02e-134"
     Typing          complement(3798..3974)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3975..4072
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.02e-43"
     Typing          complement(2309..3009)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..2047
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.064"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg aggctgtgat
       61 tctgaccccc gccaccgccc ctgcctggtg tacgaaccgt gccgaactct ggaatgccgt
      121 tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac tcgccatccc
      181 ccacgaactg ccgcaggatg ccgcacggga aaccgttctc gcctttgtca gagaaaactt
      241 tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa caaatcccca
      301 tgcgcacatc atgctgacca cgagggctat cggacctgct gggttcagcg gaaaggtcag
      361 ggactggaac gaccggacac atgccgagac gtggcgggca tcatgggctg accatgcaaa
      421 cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat acgagcgaca
      481 ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga tggaaacacg
      541 cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacaggctta acctggaaat
      601 acagatttcc cgcaccgaac tcaggaacgg agggctgaca gagcctgccc gccgtgtggc
      661 tgaccttctg aatatcgggc ttcctgacaa cgcaacttcg tacacactac gtgacattat
      721 cgaagcgttg ccgaaggaca gtaactcggc atggaaactc accagtaatg ccatgtcgat
      781 gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg aacgaaagga
      841 agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg gtttttcccg
      901 tattcccctg atgcaatggg cggcaccgga ataccgtagt gagcagggaa aaatccagtc
      961 tctgagccag caaatggaaa aattacgaca gcattaccgg aaggtggaga aacaggacat
     1021 tcccgcgagt cagatggcat ttgaaaaaca gtggcagcag tgggggaaat ccggccttgc
     1081 agctctcaga gcccagttgc ttaaacgtga agaggaattg cgccggaagg aacaggaaga
     1141 tactgaacgt agtcgcgcgg agcagctcag gaaaaatgac aatgccgtta tggattcagg
     1201 ggtgctgaga gcagtcgtta cggggtatgg cgaggctccc atgcctggta acggggaaat
     1261 gacctgttac cttctgcttc acaaccggaa cggagaatat acgctctggg ggaacgagct
     1321 ggagaaatac cgcaccagaa tctttgagag tgttgattta atgcgtgaca ggagtggtta
     1381 tatctgtgac cgaagcgaaa tcggccaacg tccgccactg caacgtgttt attcctctgc
     1441 aacctttgag caactgctga cgcaggtttg ccagacatgg ccgcaacata cccgcaatct
     1501 ccgccagccg aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat
     1561 gccatcacca gcggcaagga aggtggattt tatgcaggga cgtctgccac cgacgctgat
     1621 gccggtcatg agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg
     1681 cgtggatgac tctctgggcg gtgttatccg gcttaatggt acgctgtatc ccgcgtttgc
     1741 cgttccctca gccgataaca ggcatctggt catcagtgcg ctgaccgata aggggttacg
     1801 gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcacc
     1861 agaactgatg cagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac
     1921 accagaaaaa cagccggatc atctgttccg ctcgctgggt tttaatcgta cctgggagga
     1981 atggcagcgc gaggaagatg cacgaacaca caccaccgag aggagacatg atcgcggttg
     2041 gtcacagtga atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt
     2101 tcttcgatgt tcatcgacag ggatgaatgc aagaagcaca ctcacataac gcaagcatag
     2161 ttttgttttt ccgcccccaa aaattggggg gtcttttttc gctgcatcag agcgtttttg
     2221 acgcaatttt tcacttcaat agtaccttcg cactactaac acaaaaaaaa cgcagcaggg
     2281 agcgttttag gtgccttaaa atcgatttta gatcgtcatc ttgatctgtt tgtcttcctt
     2341 gaagcgaaaa tgcagggcta caaccgtgcg cccctttttg atcgtctcaa cagttacaac
     2401 cagatcgctt ttttcgttca gttcggcgat ggctggcttg attatcatct tattcaagtc
     2461 tttgaattgt tgatatttat cttcaacggc aagcatggtt cggaaatcat cgatagctat
     2521 cgttctgtca ccggtgctgc ggaactgctg gagcagctcg taaatgcgga tgctgtaggt
     2581 gctggaaagg ctggagacgt tcttcacgac tacccgcgta aactgcccct tgagctgtgt
     2641 cagatacggc atgatggcat cggagaaggt gatatctact ttcgcctcac ctttgaaata
     2701 ctgcgcccgg tactgcaccc agcggaactc ttctcgtttt tcctcgttct tgatgattac
     2761 tgacctgtcc cagaggcgat ctatcgcttc cttgagccta gcttcagcgt gctgccgtcc
     2821 catgtcagga aaggcatcat gaaactctgc agccgtgatg gtcatggttt tttgttcatc
     2881 cggcgaaggg gaatcttctc cggattttag gcggctgata cagaccagca ggaagcgctg
     2941 ttcctgcaaa gtgagcttgt agcttgcctc gataagggcg tttgatttgt aagctatcag
     3001 atctgacatt tcgcttgctc gttgctattg gtggcgtttt tttactgtac gccacttatc
     3061 atatgggtgg aagttttttt tttgcaaccc cctttttctg tggattacac ccccaaaagc
     3121 tccacctttc acccccaaaa gctccacctt tcacccccaa aagctccacc tttcaccccc
     3181 aaaagctcca cttatcttgc cagaacatct ttaaaatcat ggtgttacaa gacctaaaaa
     3241 ggttttaaaa aaaagatcta aaaataagat cttaaaaact taacggcgag cctgtggata
     3301 acttggggtg ctgaaaacaa aaaaccaata aaaacaatgc gttgcaataa agcagaattg
     3361 caagtttcga atacaccccc tccaaccctg tggaaaccca gtattcatgc gggttcagcg
     3421 catatcacct gccgaaaaac cgtgcgtatg tgctcaacct ggcgactgat tcacttccgc
     3481 ttcctcgccc actcgctcgc taacgctcgg tcgctggggc tgcggcgagc ggacgataca
     3541 atcactgttc tggcagcgcc tccagctcgt taatcagcgg cagtatgcgc gtcacatcag
     3601 cctgtcgtgt cacctctgcc ttcaggaact ggagtaacca ggcacgcaac tgcacctcct
     3661 ccggctttct gacctttgcc agcaatgcag cacccacaac aaccttctgc gctgtgtcca
     3721 ggctccggct cttcgccttc aggcgctgta atctcgcctc agcatcagca atcttctgtt
     3781 cgagcgttct gctcatgatg taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca
     3841 ctggtagttt aaaaactgag ctggcataat gcacggcaaa tcacgaagtg cgcacttata
     3901 caatctccac tccgtttcga ttgtgtgcgc cctgcggggc tgaaagaaaa ctacaaaaag
     3961 gcattacggc agaaatggcg atttatcatc tcagcatgaa aattatctcc agaagcagcg
     4021 gctacagcgc cgtagcttcc gctgcctatc gttccggttc actgatgctt ga
//
