LOCUS       NZ_CP069984             2042 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1291 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP069984
VERSION     NZ_CP069984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357433
            Assembly: GCF_016903855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1725.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:14:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 53 of 214
            Pseudo Genes (incomplete)         :: 163 of 214
            Pseudo Genes (internal stop)      :: 38 of 214
            Pseudo Genes (multiple problems)  :: 38 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069984.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1291"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1291"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(1760..2042,1..734)
                     /gene="mobV"
                     /locus_tag="I6K21_RS25290"
                     /old_locus_tag="I6K21_25300"
     CDS             join(1760..2042,1..734)
                     /gene="mobV"
                     /locus_tag="I6K21_RS25290"
                     /old_locus_tag="I6K21_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_204652620.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLENKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAVAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGFSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(798..1229)
                     /locus_tag="I6K21_RS25295"
                     /old_locus_tag="I6K21_25305"
     CDS             complement(798..1229)
                     /locus_tag="I6K21_RS25295"
                     /old_locus_tag="I6K21_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_024183523.1"
                     /translation="MRRITQVDQSTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQIDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="lipoprotein"
     Typing          4..731
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="99.0"
                     /evalue="3.5300000000000003e-144"
     Typing          1761..2041
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.17e-56"
ORIGIN
        1 caagccctga gtggtggaac gaggccacac cgcggcagca ggcggagttt ttcgcccgtt
       61 ctgagcaatg gctggagaac aaatacggga aagatcgcgt tgtggcggcg gtggttcaca
      121 gggacgaggc caccccgcac ctgtctgcgt tcgtggtgcc attgacgcag gatgggcggc
      181 tgtccgcgaa agagtttatt ggcgggcggt caaagatgcg tgacgatcag agcacctacg
      241 ctgaatcggt aaaaaaactg ggtcttgagc gcggtatcga gggaagccgt gccacgcatc
      301 agacagttca gcactactac gaatcgataa accgtggcac ccgtagccag gtatcgatct
      361 caccggaggc gctggagccg cgtgtactgc ggaagggtat tttcaccaaa gacgtggaag
      421 atcaggcagc cgtagccaaa cgtttaagcc aggcagtgaa cgatggcttt gcaggaaccg
      481 tcgctatggc ctctcagagc gcccagaatg caaaaagagc gcgcgagcta cagaagacta
      541 tggattcgca gcagaaacgc ctacagagcg ttacagagcc ttttaagggc ttttccagag
      601 agcagatgac cgaaatcctg atgatggctc agcggtttaa gcagcaaaac caggaaaagg
      661 aaaaacagca gcgtattgaa cgtgaaaaac agcgacagac gcgatcgcgt ggtatgggtg
      721 gtatgagtcg ttagttttga tgcgatgcag gataggtctg acgccgcaaa acgaggtcat
      781 ttgcggcatt ccagagatca ggttctgccg ccctgtataa cgctgaggcc gttttttagt
      841 gctttcttgt ggttgctgac agtacctttc cagccgaatt ggggatttag ccggtacgtt
      901 ttgctccgcc caattttcgg tccttccagt atgatcccga aatcaatcag gttttttacc
      961 gcacgactga catttgtttt ttgcatagtc agcgcttccg caatatctat ctgcgcaact
     1021 tggatgtagt tttcgtaatc cagttctgcg agcaaggcca tcagcacacg catctggtca
     1081 tggtttagct cattggcaat ggtaattagc gcggcttggt tcatagtaaa gtgcctctgg
     1141 aatgatgact tttgtttagg acgaatcact gcgacaaaac cacctaattc ctcccctgta
     1201 ctttggtcta cctgcgtgat tctccgcatc taaaaatctc cattcgggaa aaaatagtta
     1261 tctctcaaga taactaagtt atctctagag ataactattg tcaaaaactt tccgcttgtg
     1321 gcgcggcttc cgagagatcc ccttctgtat cttattactc tccgtactca tacatcggca
     1381 aaatcaggtg tttttctgcc agtattgagt gtccgaattt ctatgtgcgg ggagtggaaa
     1441 ttccgcttcc tcgaacggac agactcgcgg cgatgccgct ccctaatccg ttcttgcggc
     1501 gagcggttcc tgctctttca aaaacgatat tcattgcctg atttcgtcag gggcgagcga
     1561 agcgagtgcc atttaccctt gacgactcac cggataaaac aggcttggca gtggtggggg
     1621 gtggcgcgga gcgacgcctt ccacctgcca gactgccgtt gattttgacc ttctggcagc
     1681 ttgctgcccg aacgattttg cgtagcaaag tctagtgagt acactatcat cctgatagtg
     1741 gcccgccgga ggcatcgcaa tgcgatcccc cgactggctg gatgtacgcc agaacgaggg
     1801 ggtaagcatg gcgaattacg cgataatgcg ctgcaaaaag ctgacgggaa tgggaagcgt
     1861 ggccagcgct ttgcagcact gctacaggga gcgtgagacg ccgaatgctg acgctgaacg
     1921 gacaccagaa aactactgct ctgtgtctca gtcggcagat gaggcgatgg gaaagctgcg
     1981 ggagctgttg ccggaaaaac gccgtaaaga tgcggtactg gctgttgaat acgttatgac
     2041 gg
//
