LOCUS       NZ_CP069981            34441 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1291 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP069981
VERSION     NZ_CP069981.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357433
            Assembly: GCF_016903855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34441)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34441)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1725.19x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:14:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 53 of 214
            Pseudo Genes (incomplete)         :: 163 of 214
            Pseudo Genes (internal stop)      :: 38 of 214
            Pseudo Genes (multiple problems)  :: 38 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069981.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1291"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1291"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(33418..34441,1..1130))
                     /locus_tag="I6K21_RS24290"
                     /old_locus_tag="I6K21_24300"
     CDS             complement(join(33418..34441,1..1130))
                     /locus_tag="I6K21_RS24290"
                     /old_locus_tag="I6K21_24300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235750.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSSRKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFYGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            complement(1142..1729)
                     /locus_tag="I6K21_RS24295"
                     /old_locus_tag="I6K21_24305"
     CDS             complement(1142..1729)
                     /locus_tag="I6K21_RS24295"
                     /old_locus_tag="I6K21_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="WP_042012795.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPELDFIIDSGSLSVGRPWPNK
                     CLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDDFDMVSQ
                     EILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLLSRE
                     ESLLLHTIQSERLSTDCSLFKRQPSIESALVI"
                     /name="DUF6012 family protein"
     gene            complement(1756..2076)
                     /locus_tag="I6K21_RS24300"
                     /old_locus_tag="I6K21_24310"
     CDS             complement(1756..2076)
                     /locus_tag="I6K21_RS24300"
                     /old_locus_tag="I6K21_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001020837.1"
                     /translation="MNIHLFSEVLFCVWVIALIVILFIVVKYYRRVHYRLNSLSETIKR
                     TQGGVNKRISENRELLELIKNQHPEILDEYPWVSGWLDSQEKFLVALADKSGIDINKSG
                     LI"
                     /name="hypothetical protein"
     gene            complement(2136..2540)
                     /locus_tag="I6K21_RS24305"
                     /old_locus_tag="I6K21_24315"
     CDS             complement(2136..2540)
                     /locus_tag="I6K21_RS24305"
                     /old_locus_tag="I6K21_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218539.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDLSFELNGEVYKFNFDKNESGD
                     YIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNIYV
                     CLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
                     /name="hypothetical protein"
     gene            complement(2556..3050)
                     /locus_tag="I6K21_RS24310"
                     /old_locus_tag="I6K21_24320"
     CDS             complement(2556..3050)
                     /locus_tag="I6K21_RS24310"
                     /old_locus_tag="I6K21_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_115994269.1"
                     /translation="MRHFLKALSFVVSLLPATLCAAEIQGKVIRVLDGDTIEVKTLPAK
                     IVVYEVPIRVRLTNIDAPEKKQPFGRWSTNQLKTLLAGQSVTVSYTQTDRYGRIIGRVF
                     TTNGTDASRFMVQSGAAWVYERYNEDESLPALQREAQEQKRGLWADANPVPPWEWRYKH
                     N"
                     /name="thermonuclease family protein"
     gene            complement(3047..3355)
                     /locus_tag="I6K21_RS24315"
                     /old_locus_tag="I6K21_24325"
     CDS             complement(3047..3355)
                     /locus_tag="I6K21_RS24315"
                     /old_locus_tag="I6K21_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000647576.1"
                     /translation="MKARSVRVAGIEIIDSVKTVAFVFTETLLFMVWFSAIVYGLYRVV
                     IVLWDDPLKPQSLMEFLNFIVELYSYVLPSLAVIAVALIAVLSICKILSAIWEGRKQ"
                     /name="hypothetical protein"
     gene            complement(3458..3697)
                     /locus_tag="I6K21_RS24320"
                     /old_locus_tag="I6K21_24330"
     CDS             complement(3458..3697)
                     /locus_tag="I6K21_RS24320"
                     /old_locus_tag="I6K21_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001290043.1"
                     /translation="MSEIRFVTLASDINNIKEVYSDDELKRLLEAVNQNCPARIVLGSV
                     EKGKEVPLKWCFRTIPERISVEEINEVKIYPELF"
                     /name="hypothetical protein"
     gene            complement(3752..4057)
                     /locus_tag="I6K21_RS24325"
                     /old_locus_tag="I6K21_24335"
     CDS             complement(3752..4057)
                     /locus_tag="I6K21_RS24325"
                     /old_locus_tag="I6K21_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000147047.1"
                     /translation="MTGKNRVVLPVFLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(4041..4463)
                     /locus_tag="I6K21_RS24330"
                     /old_locus_tag="I6K21_24340"
     CDS             complement(4041..4463)
                     /locus_tag="I6K21_RS24330"
                     /old_locus_tag="I6K21_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_001353737.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPAQFEKANEVINPSLPYVPDFH
                     GVIKSEVSGKGWVYEITSLSGVQARTPTFYYALAHADRVVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(4472..6334)
                     /gene="taxB"
                     /locus_tag="I6K21_RS24335"
                     /old_locus_tag="I6K21_24345"
     CDS             complement(4472..6334)
                     /gene="taxB"
                     /locus_tag="I6K21_RS24335"
                     /old_locus_tag="I6K21_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_000847107.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSPVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLGIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESENLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLHEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLAVKDVPDLDKAA"
                     /name="taxB"
     gene            complement(6321..7349)
                     /gene="virB11"
                     /locus_tag="I6K21_RS24340"
                     /old_locus_tag="I6K21_24350"
     CDS             complement(6321..7349)
                     /gene="virB11"
                     /locus_tag="I6K21_RS24340"
                     /old_locus_tag="I6K21_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_001014668.1"
                     /translation="MNGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSQTLASFRGDEISDTKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(7351..8460)
                     /gene="virB10"
                     /locus_tag="I6K21_RS24345"
                     /old_locus_tag="I6K21_24355"
     CDS             complement(7351..8460)
                     /gene="virB10"
                     /locus_tag="I6K21_RS24345"
                     /old_locus_tag="I6K21_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131270.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSEPQEERKESKENV
                     PGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHTKLL
                     EQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGWIDS
                     HFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSINIPP
                     TMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            complement(8453..9361)
                     /gene="virB9"
                     /locus_tag="I6K21_RS24350"
                     /old_locus_tag="I6K21_24360"
     CDS             complement(8453..9361)
                     /gene="virB9"
                     /locus_tag="I6K21_RS24350"
                     /old_locus_tag="I6K21_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001353735.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKAFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(9358..10044)
                     /locus_tag="I6K21_RS24355"
                     /old_locus_tag="I6K21_24365"
     CDS             complement(9358..10044)
                     /locus_tag="I6K21_RS24355"
                     /old_locus_tag="I6K21_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290764.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REARGE"
                     /name="type IV secretion system protein"
     gene            complement(10559..11653)
                     /locus_tag="I6K21_RS24360"
                     /old_locus_tag="I6K21_24370"
     CDS             complement(10559..11653)
                     /locus_tag="I6K21_RS24360"
                     /old_locus_tag="I6K21_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001353734.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLVTVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            complement(11665..12381)
                     /locus_tag="I6K21_RS24365"
                     /old_locus_tag="I6K21_24375"
     CDS             complement(11665..12381)
                     /locus_tag="I6K21_RS24365"
                     /old_locus_tag="I6K21_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832281.1"
                     /translation="MKRVKTFMLISLLTASFYSSAGIPVAVDASPEWAVEAARWTERLK
                     QWSETAKHYQGQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLLTHEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            complement(12393..15140)
                     /locus_tag="I6K21_RS24370"
                     /old_locus_tag="I6K21_24380"
     CDS             complement(12393..15140)
                     /locus_tag="I6K21_RS24370"
                     /old_locus_tag="I6K21_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105980.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFILRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDIHIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTIMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANSQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIIKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(15159..15293)
                     /locus_tag="I6K21_RS24375"
                     /old_locus_tag="I6K21_24385"
     CDS             complement(15159..15293)
                     /locus_tag="I6K21_RS24375"
                     /old_locus_tag="I6K21_24385"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_251301673.1"
                     /translation="MTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVASMMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(15333..16541)
                     /locus_tag="I6K21_RS24380"
                     /old_locus_tag="I6K21_24390"
     CDS             complement(15333..16541)
                     /locus_tag="I6K21_RS24380"
                     /old_locus_tag="I6K21_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="WP_001339175.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL
                     VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ
                     KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNLFTHGYVL
                     GKL"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            complement(16611..16823)
                     /locus_tag="I6K21_RS25715"
     CDS             complement(16611..16823)
                     /locus_tag="I6K21_RS25715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251301674.1"
                     /translation="MSFKKSVCNCISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLQKVKTGLGADESPNDFYQNH"
                     /name="hypothetical protein"
     gene            complement(16837..17457)
                     /locus_tag="I6K21_RS24390"
                     /old_locus_tag="I6K21_24400"
     CDS             complement(16837..17457)
                     /locus_tag="I6K21_RS24390"
                     /old_locus_tag="I6K21_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(17814..18029)
                     /locus_tag="I6K21_RS24395"
                     /old_locus_tag="I6K21_24405"
     CDS             complement(17814..18029)
                     /locus_tag="I6K21_RS24395"
                     /old_locus_tag="I6K21_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(18026..18319)
                     /locus_tag="I6K21_RS24400"
                     /old_locus_tag="I6K21_24410"
     CDS             complement(18026..18319)
                     /locus_tag="I6K21_RS24400"
                     /old_locus_tag="I6K21_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_000134733.1"
                     /translation="MTEHDAICISALHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(18396..19625)
                     /gene="taxC"
                     /locus_tag="I6K21_RS24405"
                     /old_locus_tag="I6K21_24415"
     CDS             complement(18396..19625)
                     /gene="taxC"
                     /locus_tag="I6K21_RS24405"
                     /old_locus_tag="I6K21_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_000539543.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVHSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEYGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(19629..20132)
                     /gene="taxA"
                     /locus_tag="I6K21_RS24410"
                     /old_locus_tag="I6K21_24420"
     CDS             complement(19629..20132)
                     /gene="taxA"
                     /locus_tag="I6K21_RS24410"
                     /old_locus_tag="I6K21_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_139455609.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINTFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKRIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            complement(20493..20624)
                     /locus_tag="I6K21_RS24415"
                     /old_locus_tag="I6K21_24425"
     CDS             complement(20493..20624)
                     /locus_tag="I6K21_RS24415"
                     /old_locus_tag="I6K21_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="WP_001353745.1"
                     /translation="MEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEEVSNHRAGA"
                     /name="toxin-antitoxin system HicB family antitoxin"
     gene            complement(20929..21222)
                     /locus_tag="I6K21_RS24420"
                     /old_locus_tag="I6K21_24430"
     CDS             complement(20929..21222)
                     /locus_tag="I6K21_RS24420"
                     /old_locus_tag="I6K21_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000954638.1"
                     /translation="MLTEAKKMAVEMMFWPACPVDDDYCFDAIMYTSVIDRKFEDEHGV
                     YLHQVYKELCSTPELKKLLKKKAIKLIAFKYDIDLDSANKILMKNMLRRIEN"
                     /name="hypothetical protein"
     gene            complement(21237..21611)
                     /locus_tag="I6K21_RS24425"
                     /old_locus_tag="I6K21_24435"
     CDS             complement(21237..21611)
                     /locus_tag="I6K21_RS24425"
                     /old_locus_tag="I6K21_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail-associated lysozyme"
                     /protein_id="WP_000844303.1"
                     /translation="MKSVYVVGKGLIVAGNKITIDGKEHVSVENSENIVVTDDCIKIGN
                     KTVVFKGRSIKVEIHGNVNKIVTENADVSIHGNAGDVETVSGDVDCHQVNGDITTVSGD
                     VTCGDVSGDVTTVSGDINRK"
                     /name="tail-associated lysozyme"
     gene            complement(21663..21962)
                     /locus_tag="I6K21_RS24430"
                     /old_locus_tag="I6K21_24440"
     CDS             complement(21663..21962)
                     /locus_tag="I6K21_RS24430"
                     /old_locus_tag="I6K21_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650311.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLR"
                     /name="hypothetical protein"
     gene            complement(22416..22643)
                     /locus_tag="I6K21_RS24435"
                     /old_locus_tag="I6K21_24445"
     CDS             complement(22416..22643)
                     /locus_tag="I6K21_RS24435"
                     /old_locus_tag="I6K21_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(22630..22881)
                     /locus_tag="I6K21_RS24440"
                     /old_locus_tag="I6K21_24450"
     CDS             complement(22630..22881)
                     /locus_tag="I6K21_RS24440"
                     /old_locus_tag="I6K21_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(22878..23420)
                     /locus_tag="I6K21_RS24445"
                     /old_locus_tag="I6K21_24455"
     CDS             complement(22878..23420)
                     /locus_tag="I6K21_RS24445"
                     /old_locus_tag="I6K21_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(23525..24040)
                     /locus_tag="I6K21_RS24450"
                     /old_locus_tag="I6K21_24460"
     CDS             complement(23525..24040)
                     /locus_tag="I6K21_RS24450"
                     /old_locus_tag="I6K21_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            complement(24680..25030)
                     /locus_tag="I6K21_RS24455"
                     /old_locus_tag="I6K21_24465"
     CDS             complement(24680..25030)
                     /locus_tag="I6K21_RS24455"
                     /old_locus_tag="I6K21_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="WP_000127482.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALEE
                     INEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLTSV
                     AGLQFTLSKSSH"
                     /name="addiction module antitoxin"
     gene            complement(25027..25362)
                     /locus_tag="I6K21_RS24460"
                     /old_locus_tag="I6K21_24470"
     CDS             complement(25027..25362)
                     /locus_tag="I6K21_RS24460"
                     /old_locus_tag="I6K21_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001147119.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMGG
                     NFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYLQD
                     FKKRSKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            26617..27483
                     /locus_tag="I6K21_RS24465"
                     /old_locus_tag="I6K21_24475"
     CDS             26617..27483
                     /locus_tag="I6K21_RS24465"
                     /old_locus_tag="I6K21_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            27496..27900
                     /locus_tag="I6K21_RS24470"
                     /old_locus_tag="I6K21_24480"
     CDS             27496..27900
                     /locus_tag="I6K21_RS24470"
                     /old_locus_tag="I6K21_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            28035..28562
                     /locus_tag="I6K21_RS24475"
                     /old_locus_tag="I6K21_24485"
     CDS             28035..28562
                     /locus_tag="I6K21_RS24475"
                     /old_locus_tag="I6K21_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(29146..29397)
                     /locus_tag="I6K21_RS24480"
                     /old_locus_tag="I6K21_24490"
     CDS             complement(29146..29397)
                     /locus_tag="I6K21_RS24480"
                     /old_locus_tag="I6K21_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000928750.1"
                     /translation="MLMKAIKSVFNFYMLYLQPFIGVVLAGYGVWKFSGDSISALLEPA
                     SYFVIGTVVLFVWYIAWQKERSKEEEFKSSIKPEDFVK"
                     /name="hypothetical protein"
     gene            complement(29433..29735)
                     /locus_tag="I6K21_RS24485"
                     /old_locus_tag="I6K21_24495"
     CDS             complement(29433..29735)
                     /locus_tag="I6K21_RS24485"
                     /old_locus_tag="I6K21_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096507.1"
                     /translation="MAKLPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDDD
                     LDTAQVFQNKYGASRAEVIRAALAALRLVSAEERRRLFDDIRKNSPKAGRPTVNK"
                     /name="hypothetical protein"
     gene            complement(29829..30488)
                     /locus_tag="I6K21_RS24490"
                     /old_locus_tag="I6K21_24500"
     CDS             complement(29829..30488)
                     /locus_tag="I6K21_RS24490"
                     /old_locus_tag="I6K21_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516222.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCAIRKGKTACFLETDSQGSI
                     KDFIEERKTNERLSEIPYFECYTDIPAMARKLAARFDYVFVDTPGMKSPAFVKALSCAD
                     ILFTFIEPGAGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASELRRQ
                     LNDDPDWLPVPRQRIYMRTAHKKAYNSGMGVHEYDDKRGNKARGEIELLLKETDVL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(30593..31417)
                     /locus_tag="I6K21_RS24495"
                     /old_locus_tag="I6K21_24505"
     CDS             complement(30593..31417)
                     /locus_tag="I6K21_RS24495"
                     /old_locus_tag="I6K21_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_001353741.1"
                     /translation="MSHLPASIFQISRSDAWNLTLSPDAARNMRDLAAQIGAGMPKYLI
                     APEVSLLLSYIDDLNIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILKTL
                     KQRLKEAERRPGRPTTRHPPLHPDPRYRKPQEPAVRLVPVLDAGYALRMREYLATWKKR
                     IKHQPVWDITSRQTPLNWINTAVARAERDGVTFSVPVTPHTFRHSYAMHLTMSGVPPRV
                     LQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPRRAKQLLGYPERINDE"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(31973..32629)
                     /locus_tag="I6K21_RS24500"
                     /old_locus_tag="I6K21_24510"
     CDS             complement(31973..32629)
                     /locus_tag="I6K21_RS24500"
                     /old_locus_tag="I6K21_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000972912.1"
                     /translation="MMIKSKKVVVIFVALSSFLLAGCDSPKDANKQNFEKAINTRLEKE
                     CITISPLGMFDSKPYPVQVAVAQANRYTSQERADEINSRQFSALDVLVKADLLTVKDTL
                     VDDVIGFTKTGKKVPGREYALTDEGKKYLKSPERPDFCVGHYKVDEIVDFTEPGDAMGM
                     KITQVNYTFSPTSIAEWAKRDDVRTAFLGLESDLKEKQTKRITLVLKNDGWSAER"
                     /name="hypothetical protein"
     gene            complement(32733..33197)
                     /locus_tag="I6K21_RS24505"
                     /old_locus_tag="I6K21_24515"
     CDS             complement(32733..33197)
                     /locus_tag="I6K21_RS24505"
                     /old_locus_tag="I6K21_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293142.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREVDFEQLLEMQEKLNVV
                     VEERREEAEREAVERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIDKLQPGQVE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(33215..33421)
                     /gene="hha"
                     /locus_tag="I6K21_RS24510"
                     /old_locus_tag="I6K21_24520"
     CDS             complement(33215..33421)
                     /gene="hha"
                     /locus_tag="I6K21_RS24510"
                     /old_locus_tag="I6K21_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     Typing          26612..27483
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.656"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18400..19625)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.022"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4476..6334)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.871"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8457..9361)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.338"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9362..10044)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.561"
                     /coverage="100.0"
                     /evalue="4.14e-137"
     Typing          complement(10584..11653)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.11e-173"
ORIGIN
        1 gtcgggatta tagcagtatg cgcagtcacc tttgcactgt taaacgcttt gcttttacgt
       61 gaagaatcaa tatccagcga ctgaggaaag actgatttca gggcatcgat aacctgcggc
      121 gcttcgctga attgttcatc agataaatat gagcaatcag agcggttata agtaatcgct
      181 ttatattttt cacgtaattg ttgcgtaata tccagcgttt tttgtgccgt cattttgaac
      241 tttttgttca tgtattgctg gagtcgtacc aggttaaagg gcaacggtgc agccgttttt
      301 ttatcatcag ttgctgccgc ttcaactgta gccggttttc ctgcaaggga ggctgccgtt
      361 ccgtctgccc acgctttatc aagtaattta cggtctgtca gcggagcaaa ttcccctggc
      421 ttccagttcg ccctgataac atcagcacca cgctgaaaga ctccggtcat ggtgtagtaa
      481 aaactggatt tatggttctg gttagcacgg gtacgattca caatcaggcc aagaacggga
      541 gtctggacgc gcccgacaga cagaacgccc tgatatcctc tggcttttgc aggaatggtg
      601 taagcacgcg tcatggagaa gccatagacg gcatcagcaa ctgaacgcgc cagcgcctta
      661 aggtaaagcc ctttgaaatc acgattatct ttaagatttg ccagtgcctt tttcactgcc
      721 ggaagcgtgt tgtcgttaat cagaacgcgc tttacgggtt ttgtgttgcc tgcatattcc
      781 aggacttcat caacaagtaa ctgtccctca tcatcaggat cgccagcgtg aacaatttca
      841 gtcacgtctc cacgtctgat aagttcgaga atcgttttaa cctgttttgc ggcactttca
      901 acaggctgat acttcacggg ataaagacgt aaaggaagcg tttcaacctt ccaggctttg
      961 tattcaggat tatagttttc cggcggttgt gattcgataa tatgaccaaa acagttagtc
     1021 acaatggcgt tatcactttc aaaccagcca tcatggcggg taaaattgcc accaagtgcc
     1081 ttaacaatat catttgctac tgcgggtttt tctgcgataa aaagtctcat cttattatcc
     1141 tttaaataac taatgcactt tcaatagacg gttgtctttt gaataaagag caatctgtac
     1201 tcaatcgttc agactgaata gtgtgaagta aaaggctttc ttctctggac agaaggaagt
     1261 caccccattc gaactcttcc cacacatcat gactgttttc accaggttta tttaataagg
     1321 actccatttt gggctggatt ctggctggag gcttattttc ataagctgaa tgaaccctgt
     1381 tactccactt atcaaaagaa ccatttaaaa gaatttcctg tgaaaccata tcaaaatcat
     1441 catcttctat ataggtattg acttgatggt aaattaaccc catatcttca atatcccagg
     1501 tgtatatagt agtaaaccat cttaattttt tatctgtttg caaaacgagg ccattaactg
     1561 acttccttcc ttttcgcatt ccaacccata aacatttatt aggccacggt ctgcctacag
     1621 acaatgatcc actatcaatt ataaaatcaa gctcaggtat ggaaatagat ttaaggctac
     1681 atttattagc catgagatga taaagtttag gaacaatatg tatatacata gtaatttcct
     1741 ttatctttag tgtgctcaaa taagtcctga tttgttgata tcgataccag acttatcagc
     1801 aagagcgacc aaaaactttt cctgtgaatc aagccaacca cttacccacg gatattcatc
     1861 aagaatttca ggatgttgat tctttataag ttcaagcagt tctctgtttt cagatattcg
     1921 cttattaaca ccgccctgtg tgcgctttat cgtttcagac agtgaattga gtctgtaatg
     1981 gacacgcctg taatatttaa caactataaa taaaatcaca atcaaagcta taacccatac
     2041 gcagaacaat acttctgaaa ataaatggat attcatttaa gcctccttaa ttttatgccg
     2101 ccatttcatc cattatgact gtgaagtatt cagcattagt attcatcttc ataaggtagc
     2161 caatcgtaat cgtttgagtt ccagtcaatt ggaagaaatg gctctgtttg tttatggcag
     2221 gagaggcaaa cataaatatt tttctcttta tgaaaaatca tcgacttaca tttatcacag
     2281 tcgcaccaac cctcgactgg ttcagggcat tcaggtaatt ctgtttcctg atgtttacca
     2341 tacttttccc attcctcggc atcctgataa cctccccata ctgcttgtgt aaaactgtcg
     2401 atatagtcac ctgactcatt tttatcaaaa ttaaatttat atacttcgcc atttagttca
     2461 aatgacagat caaggtcagg tagtaattgc ttttcgaagc gttgttgtaa ctctttcttt
     2521 tctttggggg ataaagccat aaaaacctct atcagttagt tatgtttgta tcgccactcc
     2581 cacggtggaa ctggatttgc gtcggcccac aaaccgcgtt tttgttcctg cgcttcccgt
     2641 tgcagagccg gtaatgactc atcctcgttg taacgttcat atacccatgc agcgccagac
     2701 tgaaccataa agcggctggc atccgtgccg ttcgtcgtga acacacgacc gataatgcgt
     2761 ccgtagcggt ctgtctgcgt ataagagacg gtaacggatt ggcctgcgag cagggtttta
     2821 agttgatttg ttgaccagcg tccaaaaggt tgtttctttt caggggcatc aatatttgtc
     2881 aatcggactc gaatcggaac ttcatacaca acaatttttg cgggtagtgt tttaacttct
     2941 atggtgtcgc catcgagaac gcgaataact ttcccctgga tttcagccgc acacaatgta
     3001 gctggtaaca gggaaacaac aaaggaaaga gccttaagaa aatgtctcat tgctttcttc
     3061 cctcccatat tgctgagagt attttacaaa tggataaaac ggcgattaaa gccactgcga
     3121 taacagccaa cgaaggaagt acatagctat acaactcaac tatgaaatta agaaattcca
     3181 tgagcgattg cggctttaaa ggatcatccc ataaaacgat aacaacacga taaagcccat
     3241 agacaattgc cgaaaaccag accataaaca gtaaggtttc agtaaaaaca aaagcgaccg
     3301 ttttaacact atcgataatt tcaattccgg caactctcac acttcttgct ttcatataca
     3361 cctcatggat tacgaggaaa ctaacgccct tcggttgttc aaccccgcaa atcccggatt
     3421 aaaatccgga atctgcggcg tttgccgtta tctccgttca gaataactct ggataaattt
     3481 taacctcatt gatttcttca actgaaatac gctctggtat tgtcctaaaa caccacttga
     3541 gtggtacttc tttccccttc tccacactac ccaacacaat tctggctggg caattctgat
     3601 taacagcttc cagcaaacgt tttaactcat catcagaata aacttcttta atattgttaa
     3661 tgtcggatgc cagagttaca aatcggattt cgctcataat ttttctcctg tatatccctt
     3721 gatacccgtt ggcgcgggta tcaggtttta attaacctct gacacgacca aacttactca
     3781 tgattttcga tatagcagca ggatcagccg ttgaacactg attcagaaat gctttacggg
     3841 catcaaaagt tttactccag cgaatgcttc cttttttctt tttcagaata ttaaaaaaat
     3901 ccttttcagc acttctgcac tcactccctc caccttgccc gactgctttt ccatataagc
     3961 aaagtaccga agcgcaaggg tcagatgcat tagcatttgt tgatattaat cctaacgcca
     4021 gaaagacagg taaaaccact ctattttttc cagtcatttt tcacctcatt ttgtgcttta
     4081 atgaaaacta tttttacctg ttcaggtaaa aaacttttct cgtttcgcca ttccacgact
     4141 gctgttgccc cttccatctt caacagatct ctgattcgaa accacagccc agcatcatgt
     4201 gttgtcacta ccaccctgtc cgcatgagcc agagcgtaat agaacgtcgg cgttctggcc
     4261 tgtacaccgg ataaggaagt gatctcataa acccatcctt taccgctaac ttctgacttt
     4321 atgacgccgt gaaaatcggg gacatacggc aatgagggat tgatcacttc atttgccttt
     4381 tcaaactgcg cgggtgttgg ctctggcggt gatgaacaac cagcaaggat agaaatcaat
     4441 aaaaacacta acttcttcat catgttctcc tttatgccgc tttatcgaga tcaggaacat
     4501 cttttactgc cagataccct tttgcgacga tttcattttc aaatactttc tgtggaggca
     4561 taaccagctt tcctttcttc ctggggaaaa tatttgcaag ttccggagca accttcacaa
     4621 aatcaccaaa gaaatactca tggagatagt agaatgcctt ttcacaacga atcgggtttt
     4681 ctcccttcat tgccaggatt tcttcactaa atttcagtgt cagtagttca tttgttgtta
     4741 ccagtggacg ctcaataagg ttttctgatt cagatctcga tgttcctcca cgactacgac
     4801 ttttgctttt cgaaccgata gttactgttc gtgttcccat cgcctctgat aactcccgtg
     4861 catctttaac atcagaagca gaataaacga ttttgcaggg tgcacttgcc agtaatgttt
     4921 tactgcctgc ataactgtag atttcctcaa gctgagagcc gacctggtaa acaatcaaca
     4981 atttcagctt gtaaccagca ataagaccag atttactctt gatgtaatct acagcaccaa
     5041 ttgacgggaa ttcatccagt agaaaaaggc agtcatgctt tatttccgga tcatccgttg
     5101 gcatcttttc aagattcacc tgtataccaa gctgaaagaa aatatttgtt attttttcag
     5161 ccaacctggc attatcacca gaaatacaga aataaattgt catccgtttt ttccgcacct
     5221 gccgcaaatc aaaatcattt ccatcagtag cattcgccac attggggtta gcaaacggtg
     5281 ataatccatt aagaaagttg cttttgagtt gtgcttttgt tttatcctcc atctcaatga
     5341 atgccttaag gccagcgaca ccaatatcag caagcgtttt gtctttcccc cttaattctg
     5401 aacgaatatc attcatgaat gaaagaagat gttcgggttc tgcacgctca aaaagctgat
     5461 taattttaga cattgagaag acaggctcaa tatcaagcgt ttttagtcca gccttatcat
     5521 tcttaatcat aaaccataaa gcgacagcat aagatttaaa aacaccaccc gccgtcttat
     5581 taagatagct accagcatcc gcaccatctg tcttataggg gtaaataatc aacgaaagat
     5641 tttcaagctg gttaaaacct tcactcgttc ccatgctgac ataatacaaa gggttaaagc
     5701 ggtgcgttac cggactaaag ggatcaaatt tataaacctc atgacctaaa atcaccttcc
     5761 tgataagact ggtgatatta aaacactccc tttttggatc gagaataata acactccacc
     5821 agtacttcat aagattagtg atgactatac cagcaccttt accagccctt gttccggctc
     5881 ccatagcact gaatgctgct cccacatagc gtattatttt cttgtctttt ataccaacga
     5941 taatcccgtc tttttcgtca ccgtcaagcg tgaaactgtt agactcacga atatcattaa
     6001 ttgttgcaaa tcgcgcatca ccgaaaatat tttctttctt ctcgttcagt tttatcaacg
     6061 ccgcaacagg gatgataaga gacacgacaa atcctgctcc aagtgcaaca aaggctaaaa
     6121 ctctgatatc cgtagtcaca acagataaag taaggctgtc tgtaaaaata tccatcctgt
     6181 acgcatcaat ggatctgaac acagccttca atggtttcag attctttatc tggctgaagt
     6241 aaagcacaaa gaaaataaat gaacctgcaa accatgccgc aacaagcata acagcgaata
     6301 taaaaacaga ttgcgactta ttaaaagttt tcacattcag cccctttgta atagatgtcg
     6361 ctcatatgcc tgacaacacc atccctgcct acatgaacaa caatatcaat actgtccata
     6421 atgattttac gaagaacatc aaaaggaaga ttctgacatt ctctgttctg ataacatttt
     6481 gtcaccatct gaatgatggc atcttttgcc gaggccgcgt gaatggacgt catgcttcca
     6541 ctgtgaccag aaccagcaac tttcacaaaa tcccaggcat cgccaccttt aatttccgcc
     6601 agtaaaattc tgtcaggttt catcctcaaa caggatttga ttaatttcgc tgctgttatg
     6661 atagagccgg aatcactgcc ggattccgaa gggtaaaaaa gatgcacata atttttatgg
     6721 ataaaaaatt tgatttcctc tgcatcttca atcgtaatca gccgtgtatt caatggtatg
     6781 aaatcgataa gactcttcat atatgttgtt ttaccggaac ccgttgctcc ggcaaataca
     6841 atggttttcc ctgctgctac ggcaagctcc ataaaagcag cgatatttct cgctctgaat
     6901 aaatcaagaa gctcatcatc gtatgtcctg tgtttttttc ctgactggac ataatcataa
     6961 aaaccgttcg ccacatattc tttatgcgtt atctgccgtg tcgatggttt acgtatggtg
     7021 attgaaatag tatttctctc acatgcagga ggaaaaacga cctgtacacg ttctcctgat
     7081 tccagtgttg ctgaaagtaa tggcttggta tcgctgattt catctccccg aaatgatgcc
     7141 agcgtctgag aaaaacgttt acaaaaatca aaggtcacac tgtcattatt atggaatgtc
     7201 cagtcaccat taatttccgt ccagatttca ccaggacggt tgacagcaat ttctgttagc
     7261 ccttcaatat caagaaaaga ctggaatact tgatttttat aaaaatcgag cgatttattg
     7321 ctaatgctga caggaataac gccattcata ctattttacc tttaactcat agatatcaga
     7381 aaaatcaatg tcttccccga ccataatagt gattatgtca ccctggtttt tatacattgt
     7441 tggaggaata tttatcgaat tctcaagtgc gactttagcc atttcagcca ttgcctgtcg
     7501 tgagttttcg gtataatcca cattacgatc tttaccaggt gcattatccg ccgccgcagc
     7561 cgctacatcc tgaaccatac tgagcataag agaaccacca aaacgctccc agaagtgaga
     7621 atcgatccat ccatcaatgc ccgcttctcc cagggggcca gccgctgctg tatttaccat
     7681 atcgattcgt ttattatcag gcgtccgcaa ctgctcccac ataacgaaca ttctgccctg
     7741 tccatgattc agcgttccgg tctggtaacg accaaaagcc ttagttccct gttccagtaa
     7801 ttttgtgtgg tgattagcac tccatacatc ttctgtaaaa acacaactga tacgacctgc
     7861 aacatcagaa acaaagcgtg tggttaaagc acacggtata agcctgtttt cctcaatata
     7921 gagatcagga tcgagattaa tcgcagtcac tttcattcct gatttaccag gcacattttc
     7981 ttttgattct ttcctttctt cctgtggctc tgatgtcctg ctgctgccac tcgcgccagt
     8041 ctgactccct ccctgaccag caacaggtat tgacaaatat ttcctgaacc cctcctgtgg
     8101 cacttcttta tgaccgtcag aaacagtatt accagaacca gtagcgcctg gattgataat
     8161 tgctttgttc tcaacagggt caaaattctg accaagacca gtacggattt tacttacctg
     8221 agaaatgctg gtatcgcctg ttgattctga tgctttctgc tggtcatctg caaaataatt
     8281 tttatatgtt ttcagcgcaa aaataccaat aactaataca gccatcagac caaacagaaa
     8341 gccagaacgc atacgattat ttttgttcct gatatcaccg atatcatgaa cgggtttact
     8401 ttccagcttt gactgattcg catcatcaac taaatcatct gtttttttca tatcagtcat
     8461 tattcacctc aacacgttct accgcaggag aagatgtatt cctctggtcg gttaatactt
     8521 ttccgaaaga tttattcaca acaccaacaa cgctgtttcc atagcgtaaa acaaatttat
     8581 cattaacgtt gtggataacc ataattttat acttgccttt tgtttccaca ttaaaagcaa
     8641 gtgtctgttc ctgaccatct cttacagcaa atggagctgg gaatgctttc cctgagttaa
     8701 aacctaaaaa ggtaaatata ccattatcat aagcaaaatc aggggcgata cgacggctgt
     8761 catatttttc attgactcgc atgaaataat tccagttttt aggcgcatcg gctttttcaa
     8821 aactttcaga aatcagtttc ttctgtctga gtttttcctg tttttctttt gccagcctta
     8881 cttcttctgc attctttttc gctatttctg atggatagcg gaattgtaca acataagcag
     8941 gttgcgcttg tttttcttct gacagaagga ttagatccag actgtaaatc cgctttgttg
     9001 ttctgacaaa aagattcgta tcccattcct tttctgttgg ctggaaaacc tttttcagtt
     9061 tttcgccttc atcgccttcc tgttcctgaa caacgggacg cggattgatg taaacaacat
     9121 tatcatcagt tgtgatttcc catccggcag gaaaaccagc ctttgcactg ataacagcct
     9181 caccttcatc gaaaacaact gatgtgagaa atcctgtctt tgaccggata acggtcgtat
     9241 tcgcaccgtt ataactcaca gtctgcatcc ttgcatcaaa tccagaaggt aacggagtcg
     9301 ctgctgaata cacaagccca ggaagcaata aacatgtagc aataaataat ttcttcatca
     9361 ttcccccctg gcttcccgat ctgtctggta actggttaca gtaaaaccga gaggattaag
     9421 ctcacgactg gaacttttaa ccgacttttc tggttcaaaa cgatatgtca cacgagccgt
     9481 ccagtatgaa actgaaacct gtccggtcgt gaagtttctc gtcgtttttt taaagcgcac
     9541 agaggccagt ttatctggcg cggttgcgtc agaaataata ttggagatga cctcaaccgc
     9601 aacgtaagaa ccattattat gataaatttt atcaggagcc atatcgctgt tatacagacg
     9661 aagataatcg tcctttactg tattcgaact atatagctgt acagtctcat aatcatactg
     9721 aagagacgga taattataac cttcacgtaa ggtaatatac gtgtttacaa aataccgccc
     9781 tagtgcttca gattgcagga tcttttcctg tttaacctga gtaatcagtt ctgtacggcc
     9841 tgttttttcg ccaacagcca caatagcaag ctctttttct tttaatggca caagaacaca
     9901 aattgcagcc ccaagacata aggacagtaa tgatgctgcc actgcaacac gccatgcatt
     9961 tttcttagac cgttcatttt ctgcatagag ttgtctttcg aaggatttag ctgtctcaat
    10021 cttcttttcg attttttcac tcataactga ttacctggct gtggttaatg ggttccagtt
    10081 cgccggaaac atcagaagga agggtattcc ttgtggtaca tccggaaatc tgaaacagga
    10141 aaaacaaaag aatcacacat cgcataaatt caccttatta agcgttcacg aacaccttga
    10201 agagataaag gccaagatga agaaaaagaa tcccaccaat aagaagcaaa aagcgaagat
    10261 ccataccctt tctccatttc cccgcaagcc ctaaaccaag tcttattcct gcgaagcagg
    10321 taaacactcc taaaagaagg attaggacaa aataaagtat catgacaaaa taatgaagca
    10381 tgtcacaacc agctctgact gattccacct aagaccttaa ataaggtaat tcccgtgatt
    10441 aaaataataa gctgggttat tagctttctc ttcctgctcc atccggaggt gatatgttta
    10501 tcaacaaacg tcagtatcca gttcagggac atcacataaa aaaaacgttt cattatattc
    10561 atgcggcatt cctcgctcgt gctttttcca gggatgcaag tcttctcgcc ttcatcccca
    10621 aagcacctgt cggagccagc gcctgatatc ccttttcacc cgcccattca gcggctgttc
    10681 tcgcaccacg ccccatcaga ttccctgctt tccctgataa agaattcatg cccgttcttc
    10741 cggccatccc taagccaaaa cctaaaccag cacgaccgcc aagcatcaga gacttaccag
    10801 tagccatacc cgcagcaccc agccccatca tggctgcgcc ctgaacagca ccttcaacac
    10861 ctgcacctgc taactggaca gcaaaacctt tagctataag aaccaataaa gcccccaaaa
    10921 aaccagccat aaagcccata gcacctgtag atacaagatt tacattccct gtttgtgcag
    10981 cacgaatagc atgggaaagg atcatccctt gaaaatccat tgctatccga atcactaaac
    11041 tggcaaatag aactgtcaga atactggaga ataacgattg taaccaatta ttaaacatgc
    11101 tccgaataaa accaaacatg agacaaaaga taaatatcgg tgctgtaata atcaggagtt
    11161 tcatcgttac atctgctgtt aaaaaaacaa ccacactaac agccattaaa ataagcgagc
    11221 cagtccatat taaaaactgt ccaactacac cttgatcttt aacatatgtt gatttatcta
    11281 aagagtatat ttccgcacca agattttgcg tacttttcca taagttatct aaagtttgcc
    11341 agacactcac ccctccagaa aaaccatctt tcatgccttg caacgcatca gtagccgcag
    11401 ttaaataccc atccgcatta gttataaaca tgattatgat tgcaaatttt gatagatccc
    11461 atactaaatc ctgcaagggc gtctgagttt tactggcaag tatttgatat cctttccata
    11521 gtacatataa cgtaaccgat accgtcacta acgacataat atcattagca aattgtgttt
    11581 gataactgga gctgatttta tctaccgaat ccataacggt agaattatat ttaacgaaaa
    11641 aaccctgagc catatatcca ccttttaatc aaaagttaat ggttcagctt ctttctgctg
    11701 tttaataaag gcctcttgtt catgtgtgag caacatctca tgatctgctt tatttttatt
    11761 attccagaca tcatactgca tttttatcgc ctgcattttt aacgactgcg cctgtaatgc
    11821 gttagccaaa tcctgggatt ctttgatatc ctttgaagcc tcaattcggc tacttaaact
    11881 ctgaatctga gacattgcac ttgataactg cttattgatc tcatttccct gttcaatact
    11941 ggcagccgtt gacacaatcc gtgctttaca gagattatca ttacgttcat agcccgtatt
    12001 acacatatca aaggctccat acttgctgaa cagattttta gcctggctgc ttaatgcacc
    12061 ttcgggatta ctggtaaaat cagaaatata actgttcccc tgcttataaa tatttgtcag
    12121 ttctgattgc agattactta gttcatttgt gaatgccgca atattcctta tacctgttgc
    12181 cgtggctaac tggtctttat atgcctgaat ctgaccctga taatgtttcg cagtttcgga
    12241 ccattgtttg agtcgttctg tccatcttgc ggcttcaaca gcccattccg gactggcatc
    12301 tacagcaacc ggaatacctg cgctgctata aaatgaagcc gtcaataatg atatcagcat
    12361 aaatgttttt acacgtttca taaattcacc tgtcagattg cccgttcgag atattcagga
    12421 acccagtcat caggctccat tccttcatga taaatacttt caaaaatttc cagattatct
    12481 gcactggcag acaatatttt cagatatcca ccaagtcccg ataaatcgag tgtcgcaagt
    12541 gctgaaaatg atttaccatc ccccttttta agactgcttt ttttaatcag gaactgtctc
    12601 gacattggat caagattttt aataatatta aattcttctg gtgtcagctt aagcccttcc
    12661 acataatcat tgtaatcggc atcagggtta gccagataaa tactggtgct gcatacttca
    12721 accacagccc gtgaaatctt attcttcagt atttcatccg gagactgagt tgccggaata
    12781 accagcccgt tcagcttacg aattgttttc agcttgttat agacaaaatc gctgaatgct
    12841 tcatcctgta gccatttcca gaactcatcc aggaaaataa caagacgccg accgtccaga
    12901 agctgcgtaa tgcgatacag cagatagaaa gtaattggtg cacataccat cggatcatca
    12961 agaaactcgg tgccatcaat gccaaaatta ttgacatgct gaatattgaa tgtgtcttta
    13021 gcattatcaa aaacccagcc atgagcctga ctattagccc attgggacaa acgaaggata
    13081 atcccattct cctgctcatc ccgatcatct ctctgcataa gatgctcaag cattctggta
    13141 atgccatatt cccgcatttc gccaggagga aaatccatca cagcatcaac actttcgcta
    13201 ataagcaatc tttctcttgt cgataatctt tcaccatttc tggtacacag aatctccatc
    13261 agttgcttca caaaaatacg gttacgtttt gttgcctcca gagcaaaagg attccatcct
    13321 gtcggctcac cgctttttac ggtgtaatat tccccaccaa gcattcggat acataattca
    13381 gcccctctgt ccttatcaag aaagacacac gttaacttct tattttttgc agaatcagca
    13441 aaagtttcgg ggttattgta tttctgtaat gaacaggcaa gatatgacag aaacattgtc
    13501 ttacctgaac ccgccgttcc tataacttta gtattagcaa gattcttttc acctgtttca
    13561 tctttaaaca gtactgagtt atgtaaattc agataatacg cctgtttgct tggtgttttg
    13621 agaatagcaa ccgcttctgt ccagcagttt ttatcgcgtt tcccctggta gaaattatgg
    13681 aatgatgcca gttcgacaaa atttacattc gataccggag ataaacgcgg ccttaaatga
    13741 aaaaccgccg gaagttgggc aaaatatgcc gctggcaatg acatggttga caatgccgga
    13801 ataatgccca ggtcagtaaa gtctgccgta attttactta cagattcgtc aagctccctg
    13861 atactgtccg ccattaccat tatcgtgaaa tggtagtaac caaaggatat atcaccggac
    13921 acaatatcat tttttgcctt ttccagttcc tgcaattgag tgaccgcatc atcttctgta
    13981 ctctttaact gtttttccgc acgtttaatg gtactcaatg cttctgattt gctcatggat
    14041 gtatatgaat gcgtaataat atagtcagca tcagaatact ggagcacatc aaaaacaccg
    14101 gatgcagtct cactacagaa atctttaatt tcaatagaac gaaagaattt atttccatca
    14161 atgcggcata tctgtccggt atcatgatta aaaaatacat ccactccacc aagaacgtta
    14221 tacaccggag aatcagtgac tctgattttt tgcaacttcc ctgtcagaag ataattatag
    14281 aatgacagtt gagaagaaaa tacctttccg ttctcctcat acataccaag aagtttacag
    14341 gtaaatttat caagagcgcc agagaatgta ttaatcattt cattcattct cttcacatga
    14401 atgtgaatat catctttctt ttctttaata ctttttttgc gtttttccag tttttctaca
    14461 cgcccgtctg gtctgtagat cagcgtaaag taaagagtgt tccccttgaa tttatttttc
    14521 ttcatcgaac cataataaca atctgatatt atatcagcat atttattacc tgattttgtg
    14581 gtaaaggcat cataaaaact cgccctgcat gaatggttat aaaatgaaac gggcagtccg
    14641 gaaaatgaac gaacaagagt ggcaacctgt gagtcaatag tttctaacat ctccgcagag
    14701 attgactcaa agttaatccc catcagttgc catgtcgcta cataatcacc gttctttgtt
    14761 ttgacaatat ttttatcaac atggcttgaa tagggtatat atttaccagt agtgattcgc
    14821 tcattcagtt tcatggcatt aacaaactca tcaatttcaa cagcttcata ttgtccggag
    14881 aggaacgctc gtgcgccaaa aaaacgatta cacactgagt ttccgagcat tttcagctta
    14941 agaaagtaaa gattaaaaat gtggtcgtcc tgcttagtta tcattctgag aataaaaact
    15001 gcgacaggaa tagccagcca gatcagcttg ctgatataaa cccccgccag aaataacgcc
    15061 cctgcaacca cagtaagagg gacgacagga acacccacat acatagcagg gcgtgtcatc
    15121 gctttataaa gtgtagacat actatcgtcc ccgctttatt agttaaccat cattgatgca
    15181 acttcagccg cactggcgat aacaattgcc ccgatgatga taggtgagca ttcacgaatc
    15241 gtgcttccac caaacagaac cttgtagccg acccacagac aggcaatagt caccgttacc
    15301 agtgaaaggc caccaagccc tgagagatcc cctcataatt tccccaaaac gtaaccatgt
    15361 gtgaatagat tttgagtaag cagggttgca gccacgagtg agtcttccct tgttattgtg
    15421 tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt
    15481 ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca
    15541 agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag
    15601 ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag
    15661 gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt
    15721 tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga gtagatttta
    15781 ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga
    15841 gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct
    15901 aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc
    15961 gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc
    16021 ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg
    16081 gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa
    16141 cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg
    16201 actgaagctc gcaataccat aagccgtttt tgctcacgga tatcagacca gtcaacaagt
    16261 acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt
    16321 cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt
    16381 gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca
    16441 agtaaggcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggcag
    16501 aattggtaaa gagagtcgtg taaaatatcg agttcgcaca ttttgttgtc tgattattga
    16561 tttttggcga aaccatttga tcatatgaca agatgtgtat ctaccttaac ttaatgattt
    16621 tgataaaaat cattagggga ttcatcagca ccaagccctg ttttgacttt ttgcagcagt
    16681 gtttccgctt ttgaaaatcc atccgcaaag gccggtgatg cgactgttgc cccggcgact
    16741 gccataacta ccttattttt acgctgcatt acagcagtga cagatgaact gatacaatta
    16801 catactgatt tcttaaatga cattttataa gtcctgttaa ttaataactt ttgttggatg
    16861 tgagttatca acaaaagcag atttcagaat ggtttcgggc cagataactg acgcattgtg
    16921 tgaacgattt tttcgctgtt ctcctccgtt gcttcttgta cctggtacaa catatttttt
    16981 ttcgttccct gtataaccaa ttcgctcaac ataacttgta ttattaaatt ctttttcttt
    17041 tcgcttaccg ttactaaaat ttcctgtata gtaacaacta agcgcattct ccaggctcct
    17101 ccctcgttta taacaatcca cgataatctt ttcgaaaaca gacaagtttt cacacgaatc
    17161 aaggagtttt tctgcggacg tggaataaaa agaaaaatta ctgctggtaa tctgcatcaa
    17221 tccgacagaa taacgtcttt ttctgttttc aatttctctg ataactgtta gagcttcctc
    17281 cttacttttg ggcatatgac tgataacact tttgccatag ctaccgcgtt ccctttcagg
    17341 aataatttca gccacagcat aaggattgaa gccactctcg acccgtgcaa tatcatttat
    17401 cgtatccgga tgaactgacg ccgcacacag caacaaggct gggaataaag acgccatagc
    17461 taacgctcca tatatccatc aatacgcata ctctgcatta ataagtaaat aatttgcttc
    17521 ctcttactaa gcaaagatgt tttcgttttc tcttcgataa atgcattcaa cctgggatcg
    17581 agttttatcg tgacataaac ccctttttcc gatgacttat ctggtttctc cttcatttaa
    17641 tcaatccact ataagtgtgt tttggatttg ttttagatgc gaacatgatt cgcaaaataa
    17701 caattctcaa caatacgatc aaataacatt gacaaaatgg tcatattatg attcgtgtga
    17761 tcatgcaaaa aaaaagcgcc catatgggcg ctttagtatg atttaaacat tagttaatcg
    17821 aatgtaggga ctggagcatc cttctgagac tgaataaaca atgcttgttt aacattctta
    17881 cagttctgag aatcagagcc agacttctca cactctgcct gcttttgttt agcttccgta
    17941 agatgctgcc cccaccaatc cacacttttt acttcttcac tacaaccaac aacgaagaac
    18001 ggcaaagcaa aaagcagata ttttttcata aattccccct ctcactgatc ctggaaagac
    18061 ttgacacgag aagggcatta gtccgaatga gtaccagtgt tctgatccct gccaacgaga
    18121 cacctcccag ttcagctctt acagaatcga gatattttct gacagttgaa ggctttagtc
    18181 ctttgaaatc agcaatttca ttcagggtat agccataagc gtacatcaga ataatgtctt
    18241 tttgttgctc actcaaatgt tcttcatcac taaaaatttg atgaagtgca gaaatgcaga
    18301 tagcatcatg ctcagtcatg ataacctccc tcgtaggttg ttgtgatcag caggtgagca
    18361 aagtagccgc tttgttcacc tgcgccgaaa tcaaattaaa atcccaggcc tttccagttc
    18421 ctctcttgct gtattcttag ttttttatgt tcttctgaat cctttgtaaa cccgtattca
    18481 agagcaaaac cgatattgtg accttgctca tgttttctgg atagctgttt tttaacgtcg
    18541 ctctcttccg gttgcctggt ttcacgctca tgaacctgac gttctctgtt ccgctcaagg
    18601 gcaatatttt caatctgcca gttattgcga tgcgtttccc tgtagcctgc aacagttcca
    18661 tcatcattaa acatcgggct atgtacaagc gtgggagcca gttttttaac tttgataagc
    18721 gttcctggct ggagcttttc agactcgaaa tgatttttca ggtctttacc ccaaatcgta
    18781 acctgttttc caccttttaa agtttcataa gtgaggaaag gtgttcgttt ttcccctgcc
    18841 ttattctgat atgaagtctc cccaaaatcc acaaccttgt aaacattccg gagtcttttc
    18901 ggattttcag cctgtaatcg ctcaatctcc cgtttacgtt cgggatcttt tttccaggta
    18961 gcagtaacat catatcccat acctttcagt tttctatgaa aaccgtttct gaacttcctg
    19021 gtttcagatt tggtaagtct taatcttctt cctgtttctt catcacgaag tttaatatta
    19081 acatgtacat gaggatgatc ttctttatca tcatggtata cggcaacgaa agcatggttt
    19141 ggataagtct ctttaagaaa ttctaccgta gctttcagag cgtcttccct gcttactcca
    19201 gcttctggtg gtggtgaaaa gaccatgttt ttcacatgat cgatgttttc tccacgatat
    19261 gtcctggagt aatcatttcc tttagataaa gttgaagtaa actccctgtt aaagtcttct
    19321 cctttccctg tttgctctga accttttcca tcataacagt aagcctcaag ttcaccatta
    19381 cgggtaatgt aatcaatacc gtttttgata cctgcggctg ttttacctga gccagtgatt
    19441 ttaaaagtga cttcttttgt aggtgtccag gtatatgctt tcttgtccgc accatgtctg
    19501 actttatgat taaaagccga tttactggca tgtatatgtc ttcccgataa cttaggagat
    19561 ttcggatcac gcccagtccc tttagcgcgt ttaatacggt attcctgttc tacatacaca
    19621 cccataaatc acccaaatct gttaacaaca ctcttggtta acgtttcaag ttttgaatca
    19681 attcgcttac acagttcaat cacttcaata acgtcttttc tgaaatctgg atcgttaacc
    19741 gtacagaatc tttcaccttt tatgataaaa tgaatgttgc gaccaacacg atctacagca
    19801 gttgatagtt ttttaagttc cttaacttca tcagggtaaa gacagacttc actctttaac
    19861 aaagactgac gcacacgaat agcaatttct ctgctcatgc tcatgccgtg aagagcagca
    19921 ttacgtttaa caagttctat ttcatgaggg taaagacgca ctgataccag ctttgctggt
    19981 tcattattaa ctgggatgtt aacaggcttg gtttcaatat taagcacgtt atgcttagca
    20041 aaagtattga tactggtaaa tccacttaaa atagcgttat gttctgcaaa ttttaactca
    20101 tcatcactta gcctgaacgc tatcatcttc ataagtcacc ccaacagagc ctgcggcagg
    20161 cacaggcaaa aacatcgttt ttggacaatc tgattcaaat gttaacattt gacatcggat
    20221 tgtctatcct gcattgattg taccatatgt agcactgtgc ctagctataa aatcgtcgct
    20281 gcatatgtcc agatttcgtt gctgaaaggt tctttcgtct tcgaccatct ggcaaacgta
    20341 tagctgaaag atttgtgaaa gaaaaaacag gcctggcagg gctgggaatt gttacggctg
    20401 aagccgtaac gctttttaag tcctcgctac gctgcggggc ttccggcgca aagcgcctcc
    20461 catgccctta cagggcataa aaacaagggc aattaagccc ctgcgcgatg attactgact
    20521 tcttcttcca tcgccatttc tgctacccga cgatgcagtt ctggatcaag cctgacgtta
    20581 aacacgcctt taaacggggt atcaggttcc tttccatcct ccacacaaga ctgtaaatac
    20641 agatcaaccg atgttttaaa ctcctgttcc agttctgctt acaaaggggc agaaagcccc
    20701 ttttttattt aaaacatttc atacccttgc aggtagttta accaccaccg gaattgatcc
    20761 cattcgctaa atttcattgc cggaattctg gtttcccagc ctttgacaag ggagtcagca
    20821 agctgtacga tctgccgcgt tgacatttaa aaacctcatt cagttttgat tgtcgataac
    20881 gtgatcaggt tgttccagca acctgatcac tttcactttt tcagtttttc agttttcaat
    20941 cctccttagc atatttttca ttaaaatctt gttagctgaa tcaagatcaa tatcgtactt
    21001 aaaagcaatc aattttattg ctttcttttt tagaagtttt tttaattcag gtgttgagca
    21061 tagttctttg taaacctgat gaagatatac cccatgttca tcttcaaact tcctgtcaat
    21121 aactgatgta tacattatag cgtcgaaaca ataatcatca tctacaggac aagcaggcca
    21181 gaacatcatc tctactgcca tttttttagc ttcagtaagc atatccgatt ccgtgatcat
    21241 tttctgttta tatctccgct gacagtggtt acgtcaccag aaacatctcc acaagtaaca
    21301 tcaccgctta cagtggttat atcgccatta acctgatgac aatcaacatc accactcacg
    21361 gtttcaacat ccccagcatt cccatgaata ctgacatcag cattttcggt tactatttta
    21421 tttacattcc catgtatttc taccttaata ctcctgcctt taaaaaccac tgttttatta
    21481 ccaattttaa tacaatcatc agtgacaaca atgttttcac tattttctac cgagacatgc
    21541 tcttttccat cgatggtaat tttattaccg gcaacgatta aacctttgcc aacaacataa
    21601 acacttttca taaaatcaac tcctttcagg aaaatgcagg gatgcccctg cattcataat
    21661 gcttaacgaa ggcactcaat aaaatcttta aaacttaagc attcatcgcc atcttttaac
    21721 gaatcaaaat atttttcata ttcgtccctg tagaaaagga ctaaatcaac atcactctgc
    21781 ttaagttcgc caaactgagg aacaattccg gttttatagt cacgaatttt attgttatca
    21841 gtaaatatga caacatactg ttgtcccgta tccttatgca taaggataat agtgcaaagc
    21901 gttactcgtt cgaacgtgaa aacatgatta gtggttgaag taaaaacaga tgagcatttc
    21961 attttaaatc tcctgccctg ctgggctatt gtgagagggg ccattcccct ttcgatgaaa
    22021 taataatacg acttttaagt cgtatttgca agttttctcc cgttttttta ctcgataagt
    22081 gattgttatc acgtttttta atccgcgtaa gcggatggaa ggggcgaacg aaaataccag
    22141 ggcagcacag gagccacatc gccccgaagc gcggcgcagc cgctgacgaa cgtaccaggg
    22201 ggcacaggcc atcccttcac cctgcaaagc ccaactgacc agcaggccat gattatgctg
    22261 atttgcgccg cagaatgccg cagttagcgc gagcgccagc gagtttaccc cgccctggtg
    22321 cattgcagca tggcggggcg ctgtactgcg cgtgtgagcg tcagcgaact acgggcgcaa
    22381 ctccaggtac taatataaaa ccatcacagg tcattttatt ttgactccct tttgcagagc
    22441 acgtattcag gatgttctcc tgcaagtaac aaaagaaact caaactcaac cagtgacaga
    22501 ctttgactgt tttgagtatt aacttcctct tttttctgcc aggttcttag cccaacacca
    22561 taaatatgac tgcattcctt ttgtgttaat cctgctttta aacgtaattt ttttacattt
    22621 tcaggtgtat tatcgggttt catttaacat aaactcctta attaaattct taactggcgt
    22681 ctcatcgtct tcccttaatc cggcacacgt aaaatcaata tgttgtaatt cattctcatt
    22741 cagtacggca tcatacaatg taattttcgt agttttgttc ccgattttaa catttgttct
    22801 ggcttttatc ctttttgtaa gcctgtaatt gtcaccttct ttttcaagaa aataagtcac
    22861 atcaccttta ttttttctca taactgtttc aaataccctg taattttaac aagttgatct
    22921 atactcaagc cccgaatctg taaatcataa ctgtcgccct gttcggtgac tttaccgttc
    22981 aatccgtttt cagctttaaa gcctgtcaat gccccatacc agctttcaag ctggacaagt
    23041 tgcgataaca tacctttcag gatcagaatt tcgtcctttt tttccacctg gtgtttctca
    23101 tactcaatcg cctgtaacac ctcctgcgaa gcatttacca gaatttttcg ctcgatatcg
    23161 gctgcaatat caacgggatt ccgttttctg gataacgtgc aaacatgatg cggactacgc
    23221 caggagcgac tatccacact ccccacaaca gaaaaccggt ctttttccat ccgaatatgc
    23281 acagagtaat ttttaaactg cggtgacgtc agttttatac gagacgaaca atcatcaagt
    23341 ttgttcacgc gccagccgtt ccccaggata cggcagacaa cttcaaagac agggtgatat
    23401 tgttcaaaaa aagagttcat ctgtttccct ttttgctacg tagctgctgt tcccgtcttt
    23461 ttgggttcga tgccccaaaa gacgggctgg ctataatatc gggggcttct cgcccccgtt
    23521 ctggttacgc tctggtttcc acgcgttgcc accctgtcgc cctgcgctgt ccggttgtac
    23581 cgtattttgc gcggatttct tctatcgtgt gttcttcacg attccagtaa cttttatgct
    23641 cgtctggacg ataaaaccat tgttttttct ttgccgccca tttacacccg atttctttta
    23701 aagtttcctt atgtgtaatg gtttctccac taatccagac ccagttacct atcacttcaa
    23761 ataccagacc agataaacca gaaagaacgt ttaatacttt ttccaggtca tcaccgtaat
    23821 tatatcgtgc atgttcatca gtgctctgga actgatttat tttatcaata tttgccatca
    23881 atacatcaaa agctgcattt accgctttca tcagttcagc ccctaacggg ttacgatctg
    23941 gatgatattt taaagcggct tttctgtatg ctgctttgat atctttttca gttaattcgc
    24001 cggataatcc aaaaacgttt aatgcttcct ggatattcat ttttttaatc tcctgccctg
    24061 ctgggctatt gtgagagggg ccattcccct ttcgatgaaa taataatacg acttttaagt
    24121 cgtatttgca agttttctcc cgttttttta ctcgataagt gattgttatc acgtttttta
    24181 atccgcgtaa gcggatggaa ggggcgaacg aaaataccag ggcagcacag gagccacatc
    24241 gccccgaagc gcggcgcagc cgctgacgaa cgtaccaggg ggcacaggcc atcccttcac
    24301 cctgcaaagc ccaactgacc agcaggccat gattatgctg atttgcgccg cagaatgccg
    24361 cagttagcgc gagcgctagc gagtttaccc cgccctggtg cattgcagca tggcggggcg
    24421 ctgtactgcg cgtgtgagcg tcagcgaact acgggcgcaa cgcgccagaa tctgctctat
    24481 gttaatgcta atgtatgcat acattagcat taacatacgc atctatcaca tcgtctttaa
    24541 atgtgtatta atacacaccg ttaaactatt gccagtacgc aattgtacga tttgccgatt
    24601 aaataccggt gaacgtcggg cgggataact gcggttttga tggcatggaa attcccccgc
    24661 ttagggggaa ctggagagat tagtggctgg atttactgag agtgaactgt aatccggcta
    24721 ccgatgtaag ttgtgcaagc gttgtgatgg tcgggttacc gttgggggat aatgcgcggt
    24781 aaatactctg acgggcaagg ccagatttcc tggcagcttc ggaaatcccc cctcgcgcct
    24841 cgattacctg acgtagcgca acaagaaaac cacctaaacc accaggctcg tttatttctt
    24901 ccagggcatt agcaagataa gcgtttgcat aatcaggatc tgcgcgcaat tcttcaatca
    24961 tggcggcatt gtgatcaacg gatgccggat aatcattttt ctgcgacttc acatttttgg
    25021 tggctgtcat ttttttgacc tctttttaaa atcttgcaga taactaacgg ctttatctaa
    25081 atcggctgtc tgagtttttt tattccctcc aatgagcaga agaattattt cgccatcttc
    25141 aattgaataa taaaccctgt aaccaggacc gtaatcaacc cgtaattccc atacaccgtc
    25201 cctttcgaat ttatgatctc caaaatttcc gcccatagcg cgggcaattc tggaatccac
    25261 ctttaacgct gcggtctggt ctttacgtct taattttttc atccagtcgg tgtacggaac
    25321 ctcaccgttt tctgtctgat atcgtttcgc cgtgtaaggc atcttgccgc cctcattccc
    25381 ttaacctgtg tacattgtct cttataagtg acactagatc aagaaaaatg tctctttaaa
    25441 gtgacaaacc ttagctttct ggaaggttct gtaacgcatt ctgagcattt tttccgtttc
    25501 attgtactta gccctagtct taacagcgtt taacgcctct caacgctttt tagcagcctt
    25561 gttgctgtga tctgttgtca tgatccggat agtagttaaa tcttgatcaa aatttcttca
    25621 aggcaacgtg tgttttgtga atacccctta aaacgcgctg tatcgcgttt taagcgattt
    25681 tttcatttcg catggttttc ctcatggttg tatcgaacga agcggcagag cgcgattcag
    25741 ggcttcttag tggaagatcc aaaaaggatc aaataacgga tcatatgatc tgaatctctc
    25801 tggttttgag aaaggagata ccgctcgccg cagccgaacg gcaggagcgt agcgactgag
    25861 tgagcgagga agcggaattt ttgcgatgtg acattaggat cagtacgcac gaatattata
    25921 tttatttcaa taagttaaag ttaacgaggg acaaatttat ggtcttgaaa attgagattc
    25981 ataaggcatc aaatattttt ttgatgattt taagttattg aaagttatga gttttaatga
    26041 ttgatgatgt ttgttttagc ttgcgggtaa taatcatcgg gggacagatt tatggtaagg
    26101 aaccatcatc gttgctggtt tcgttgagtt atccacagtt cctgaatatt cgcacgcatt
    26161 aaaagcctta tcttttcttg tttttcttaa aaacttaaaa acttagagta gctctaattt
    26221 attgatttta agcgtgtttt ttgtctaaac atgagagctt cctcggttaa acatgagagc
    26281 ttcctcggtt aaacatgaga gcttcctcgg ttaaacatga gagcttcctc ggttaaacat
    26341 gataacttcc tcggttaaac atgagagctt cctcggtgat atccacaaaa tgtgtcataa
    26401 gaatactaat tcgttgttct ttctggtgaa tctattttga atctgttgtt aatctgcctt
    26461 gaatccagtg tggattttga gcatggatta cctttgtgag ataaatcaca gatatgaaac
    26521 atgagaagat actcggttaa acatgatatt gattgtgttg taatatcatg tttctggtgt
    26581 taaagtctga ttatcaagag tgttggaaca atgagaatga cgacaaataa gacttccctt
    26641 tctcgcttaa caaaagtgag acatcgaaat gaattgaatt caacgctgtc aacattgcct
    26701 atggctgcta aaaaagtctt atttttagcc atgtgccaga taaactctaa aaatgaattc
    26761 gatgatgatc acatatttta tgtgacagtt gccgattaca ttaaatgggt tcaggttaag
    26821 cctgatgcgg cttatcttgc tttgagggat ggctctaata tattagatac gacgcttctt
    26881 aagctgaagc atgatgaaat attagaactg agtagtgatt tgggatttaa gttcactaaa
    26941 agtaatgtac ctgattcaat gaatttgagt ctgactgttt tttctactta ttacaggaat
    27001 gaaggtagag tggggatcaa gtttacaaaa gaagcgaaaa gatttttatg taaacttatt
    27061 ggtgaagaaa aacgttatac aacgcaagtt ttattatctg ttgttcgact gacaagtgtt
    27121 aatgctgcat cactatatca actcattcgt aaaatatata gtaataattc tcgcgcgaat
    27181 agttttgaaa tgacaataga tgaacttaaa gatgaattga atctttatac aataggggcg
    27241 gggggggtga aggattataa gtatccagat tatcctgctt ttaaaagaga tgttcttaat
    27301 aaatcagtaa aagaaatcat gaaacatacg gaagtaaaga acttaagttt cgtagtttca
    27361 gaaaaaattg ggagaaaagt ttataaatta aaattctctt atacaattgg ttatgagggc
    27421 gataccaggg aagactcaga gtttactaat atgtttgata aaatgtaccc acctgaaaat
    27481 tgaggtcata acaatatgaa ggataatatc gtagattttc aaagaatcag ggacagcatg
    27541 aatgatcatt caaccgatgt tgcaaatgat gccgtttatt ctgacaaaaa gaaaatatcc
    27601 aggttctttt atctgctgac acctgtcaga taccttctgg ctgcttccct ttatctggtt
    27661 gttgttattc cattggggat tcttgcggga tttcgtttct tgttgcagtt tttaggcggt
    27721 atcgcagtga tgggtttaac tatcgcctgg tacatgggtg cagatgtcag tagttacgtt
    27781 gttcttgctg catggatcgc cttaactgtt tgtggttgcg cagagcagat ttttgaatgg
    27841 tggatcgaaa gccgttttgc gttccgtctt tttggtatcg ggagaacgcc ggaagaatga
    27901 gcgccgatct tacagacagt aaagaacaat gcaggataga caatccgatg tcaaatgtta
    27961 acatttgaat cagattgtcc aaaaacgatg tttttgcctg tgcctgccgc aggctctttt
    28021 aaaaggaata tgaaatgata tttaaccgta ataaaaaacg agatgaacaa cataattctg
    28081 ctgttgtttt gccatcagct gaaaatgtat tctctgattt tattaagaac aatccagctt
    28141 tgacaaccaa tgcttttaca gccattgggg aggttgattg ttttactaaa gtacccttac
    28201 tctcaaataa tgaatcagag tttttaggca ttctttcacg taatattata cctgattatt
    28261 ccgtttttcc caaaatacgg ctcattgaat ttgttcgtcc tcatggtagt gaggaagctg
    28321 taaaagattt aatccgtgaa gtgcagaata taacctgtga ttttgtcctg aaatcatatg
    28381 atgaaacagt cctggctgtt atccagtttg gtgaaaccgc aaccgtcaga cagcaaaaga
    28441 aaaaactgat tatccagcgc gtgtgtgcta tgacaggaat ttcttttttc gaatttaaaa
    28501 acactgtcga tatgatgggg agcgaagatt tttgtcagtt cctgcgggat gatgaagagt
    28561 aaaaatatta gaaattaaaa ataagcccac atatttgcgg gcttaacaaa cgcattgtca
    28621 taatttattg tctgttatcc tctaaaataa ttaatgatgg tcctaacatg gttgtaaata
    28681 ccaatgctgc tatgccaatc ataccgtact tgtttggtac acatgataat aataaagagc
    28741 acgtagatac ggttatataa aatattctga agttactttt tttaagtagt gatgatatat
    28801 atattataca taacgctata agactaaacg cgattgaatt tatcattgta taatcctctt
    28861 ttaattgatc agcacatatc gatcttaccg cccagtaaca taaaaatgaa aaccgttgtt
    28921 actgggagca acattgccag gatgaaaacg attagattac ttttcctgcc aatattgaat
    28981 actataccag ccagcactac taaaaagaaa aatgcaacag gtactgtcca tgtaatgaca
    29041 ggatgatagt ttataatgcc taaatcaacg atgcagtgtt ccatagattg aaaatcccct
    29101 aaagtgccct aatgtctggt tataagctgg cattattccg tatcctcact tcacaaaatc
    29161 ttctggtttt atacttgatt taaactcttc ttctttagaa cgttcttttt gccatgctat
    29221 ataccataca aacaaaacaa cagttccaat gacaaagtac gatgctggtt ccagtaatgc
    29281 tgagatactg tcacctgaaa atttccatac accataaccc gcaagaacca ctccaataaa
    29341 aggctgcaaa tagagcatgt aaaaattgaa tacagattta atggccttca taagcatatt
    29401 tgtactcctt taatggtgtg taataaaaca cactatttat tcacggttgg tcttcctgct
    29461 ttcgggctgt ttttccttat atcatcaaac agcctccggc gttcttctgc tgataccaga
    29521 cgtagcgctg ctagtgctgc tctgataact tcagcacggc ttgcgccgta cttgttctgg
    29581 aacacctgcg ctgtatccag atcatcatct gtcatggtca gatttatacg cgttactgcg
    29641 gatgccgatg ctggtttttg tgcagcagaa cttacaaact ggcgtaaatc cagcggttta
    29701 atttcctgat caattttctt tgggagcttt gccattagat tcggtatcct gtttttagta
    29761 aggtgtgtaa ttatacacac tattaagggt gtgagtatat catcgcttat agtgtgtaat
    29821 aatacacatt acagaacatc cgtttctttt aacagtaatt caatctctcc acgggctttg
    29881 tttccccgtt tatcatcgta ttcgtgtacg cccataccac tgttataggc ttttttatgc
    29941 gcagtacgca tatagatacg ctggcgggga accggaagcc agtcaggatc atcattgagt
    30001 tgtcggcgca gttctgacgc ttcactgtct gacggattag ttgaacactt attcaggaca
    30061 atccatgctt tcattgatgg attgactccg gcctgtgctg ttttaatgtc gaaaaccagc
    30121 cgtcccagcg tgttgatctc gatgcctgct cctggctcaa tgaatgtgaa cagaatgtca
    30181 gcacaactca gtgccttaac gaaggccgga gatttcattc ctggcgtatc aacaaacaca
    30241 taatcaaagc gggcagccag ttttcgtgcc atcgccggaa tatctgtgta gcactcgaaa
    30301 tacggtattt cagacagccg ctcgttggtt ttacgttcct caatgaagtc cttgatgctt
    30361 ccctgcgagt ctgtttccag gaaacaggct gttttccctt tcctgatggc acagacacat
    30421 aaggaacaaa gcacggtgct tttgcctatg ccgcctttat gccctgcaac tgtaatgatt
    30481 tttcccatca caaaaccctc ccggttaaat agtgtgtatt aatacacaat cttttgtggt
    30541 gaaagtataa ccgtactatt gcgtgctggc aacagtttaa tgatgtgtat tattactcat
    30601 cattaatgcg ttcagggtat ccgagaagct gttttgctcg ccgtggatcg caggtaaagg
    30661 acaggccact tcctgtcagg acgtccagcg aaaaaacgcg ggtgtaaatc tcggtgcttc
    30721 ttgcataacg gtgtcccagc agggattgca ggacgcgcgg aggaacgcca ctcattgtca
    30781 ggtgcatggc gtaactgtgg cgaaacgtgt gaggcgtgac cggaaccgaa aatgttacgc
    30841 cgtcacgctc tgcccgtgcc actgcggtgt ttatccagtt cagcggtgtc tgtctcgatg
    30901 ttatgtccca cacaggctga tgtttgatcc gctttttcca ggtcgccagg tattcccgca
    30961 tacgcaatgc gtatccggca tccagaacag gaaccagcct tacagcaggc tcctgtggtt
    31021 ttctgtatcg cggatcagga tgtaatggtg gatgcctggt ggtcgggcgt cctggtcttc
    31081 tttctgcttc ttttagacgt tgcttgagtg ttttcaggat caccacgggt tgcgggtact
    31141 gccgcgtcgg agtcagcata aaatcggcag gtttcagtgc cagagcttcg ttcagtcgtg
    31201 ctccggtatt ccagagcgtc tcaaaataca tacggatgtt cagatcatcg atgtaggaga
    31261 gaagcagtga gacttccggt gctatcagat atttcggcat tcctgcgcca atctgtgctg
    31321 caagatcccg catattacgg gctgcatcgg gagagagggt taaattccag gcgtcgctac
    31381 gtgaaatctg gaaaatgctg gcgggtaaat gactcaattt tttaatctcc gcacctgttt
    31441 ttttggacgt gccagcaatt atgacctgaa gcagaggtgc gtccgacaac aaaaattccg
    31501 atcaccaaaa aaatttttgg acattttttc cgataatgca tttttgtcca gggggatatg
    31561 aacgacaggg cagggcgaaa aggaagcggt tatcctgaat aacccaggat atgggcacat
    31621 agaccggaac agggcaccgg ataaaggaac ggaacatgtg aatgttgtaa ctaatgaaat
    31681 ctggacagaa tcacctgtat gtctaactgc ctggcgaatt aaaccggagc aagggtaatc
    31741 ttgttcaaaa atactgtagc ggtcgcagct gaggatcgcg ctggtcagcg cgatcgatgc
    31801 gtcaccggcg caaagctgat ttgttgtttt acagtaagtt aggttgtttg cagtgtgaaa
    31861 aatatcggtg agtatttttg atagagtcat taacggcggt gcgctacgtg aaatccgtga
    31921 aaaagtaggg gatatacgtg ggggattttt cacgtagcaa caccattaca gcctaccgtt
    31981 cagcagacca tccatcattt ttcaaaacca gtgtaatacg ttttgtttgt ttctctttca
    32041 aatcagactc aagtcctaaa aatgctgtcc gaacatcatc gcgtttcgcc cattcagcga
    32101 tactggtggg actgaatgtg taattcacct gcgtgatctt cattcccata gcatcaccag
    32161 gttcggtaaa atcaactatc tcatccacct tataatgacc aacgcaaaaa tcaggccgtt
    32221 ccggagactt cagatatttt ttcccctcat ctgtcagagc atattcacgg cctggcactt
    32281 tcttacccgt cttagtaaaa ccaataacgt catcaaccag ggtatctttt actgttaata
    32341 aatcggcttt gaccagtaca tccagcgcag aaaactgtcg agaatttatt tcatctgccc
    32401 gttcctgaga tgtatatcga ttagcctgag ccacagctac ctgaaccgga taaggcttac
    32461 tatcaaacat tcccagcgga ctaatagtaa tgcattcctt ctctagtcga gtattaatag
    32521 ctttttcaaa gttttgctta ttagcatctt ttggtgaatc acaccctgcc agcaagaatg
    32581 aactcaaagc aacaaaaata accactactt tttttgattt gatcatcatt ttccctcttt
    32641 atcaaacgat aaaatttctt gccagcgccg ctggctgttc tgtcccctcc atttgccgtc
    32701 gcaaacttgt ttgcgacggc atcacgatta ttttattcaa cctgcccagg ttgcagtttg
    32761 tcaatgagga aatcttccat gaaatggccc tcatcaagca gcttctgtaa agcaagcgga
    32821 cgcttgccat taccagacca ttctctgatc tcgccatttt catccttaaa gcggtattta
    32881 gcctttctta ctccgccttt ggctttccgc ctggttttta ctgtatctgc tgttacagga
    32941 gcaaggagtg attcgggatc aagacctaaa gatatgatgt attcaatggc ctgctgacgt
    33001 ttggcttcaa gttcttggcg ttcaaccgct tcacgttcag cctcttcacg acgttcttca
    33061 acaacaacgt tcagtttttc ctgcatttcg agcaattgct caaaatcaac ttcacgggca
    33121 aaaacacgca atgaacgaat attcatcata gtacggctga taatgccgta ttcatcttct
    33181 ttagtcagtt cgctcatata attaatcctc ttatttagtg tacgtacttc cagacgccgg
    33241 aaggcacctt atcataaagt ttattcatca ccagttctgc tcggcgatga tctgcggcag
    33301 aataaaatgc ctctgattct gataaaggca gtttgtaacg gttaatctca ataacttttt
    33361 caagagtatc tcttgatgta cattttctta actgaaaaag ccattcctgc tttgttttca
    33421 cttcttagcc cttgccggaa aacccagttt ccctgttttc ttatcctcaa ggacaagaac
    33481 tgtatcatac gttccaccac ttttcttttt aaatccctta atcaaatcgg ttttccctga
    33541 tttaaccagt ttttcggcct gtgcttgggt gattttctta ccataaaact cactccagat
    33601 tttaaattca catccggtac agaaataacc tttcggcctg ataacaatat gtttaccaca
    33661 ggaagggcag ggagagtcca ggcgttgaaa ctgccctgct ggcgcagcgg gttcaatctt
    33721 catctcgccc aggtcaacat cagaaatcat gcctgtcagt tcaccgtaca gatcattaat
    33781 aaactgttca accgtcagat cgccattttc tatggcagcc tgcttttcag accataatgc
    33841 tgtcatatca ggattaaccg ctataccagg cagggcatca ataagcgcat atccggtatc
    33901 agtaggaata agtttccctt tttccagcgt gatatagttt cttttcttca gcgtttccag
    33961 aatggctgcg cgggtagctg gcgtaccaat gccaccatgt tcatcttttt tgtctttatc
    34021 cttatccttc aacaattttt taatcgttgg gtcagtgaca aaatccgcga cacgaacaag
    34081 cgcagcaagc aaggaggctt cggtgaataa cggcggcggt gttgttttct tctcattaac
    34141 aataacttct tttgtcgtca gtgtttctcc tgtgcgaatt ttacagagca gttcaaaagc
    34201 ggaatcatca ttatcttctg attcaccttc gtctgtggtt tccgcgccaa gaaacgcctc
    34261 aaatccgctg tcagttgttt ttctggcacg ggcatagaac gactcatcac cacactgaat
    34321 ggcaaccgat acttcctggt atgctttttc aggcatgaac tgaacaagat agtgttgtgc
    34381 gatcgccagg taaacattgc gctcgtcggt gctgagtgcg ttaacatcag gcacactggc
    34441 g
//
