LOCUS       NZ_CP069965             5463 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1307 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP069965
VERSION     NZ_CP069965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357449
            Assembly: GCF_016903775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 981.01x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:32:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,565
            CDSs (total)                      :: 5,442
            Genes (coding)                    :: 5,308
            CDSs (with protein)               :: 5,308
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 63 of 134
            Pseudo Genes (incomplete)         :: 72 of 134
            Pseudo Genes (internal stop)      :: 27 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1307"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1307"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(712..1836)
                     /locus_tag="I6K37_RS27460"
                     /old_locus_tag="I6K37_27455"
     CDS             complement(712..1836)
                     /locus_tag="I6K37_RS27460"
                     /old_locus_tag="I6K37_27455"
                     /inference="COORDINATES: protein motif:HMM:NF013211.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_139766627.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKTGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(2057..2590)
                     /locus_tag="I6K37_RS27465"
                     /old_locus_tag="I6K37_27460"
     CDS             complement(2057..2590)
                     /locus_tag="I6K37_RS27465"
                     /old_locus_tag="I6K37_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021539385.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLPEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            complement(2593..3519)
                     /locus_tag="I6K37_RS27470"
                     /old_locus_tag="I6K37_27465"
     CDS             complement(2593..3519)
                     /locus_tag="I6K37_RS27470"
                     /old_locus_tag="I6K37_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3696..4094
                     /locus_tag="I6K37_RS27475"
                     /old_locus_tag="I6K37_27470"
     CDS             3696..4094
                     /locus_tag="I6K37_RS27475"
                     /old_locus_tag="I6K37_27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(4355..5374)
                     /locus_tag="I6K37_RS27480"
                     /old_locus_tag="I6K37_27475"
     CDS             complement(4355..5374)
                     /locus_tag="I6K37_RS27480"
                     /old_locus_tag="I6K37_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     Typing          complement(4600..5374)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(2597..3519)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtttttcag tcgcttacac tacccccaac aacaaaacct gtcgagcctt cccgcaaaac
       61 cgatccattt accataactc ccgttgtacg taccccttaa aacgccctca gtcgctttct
      121 ggcgcaaaaa acgcaaaaag acaaggaaac accaggccaa accagaaaaa tgcgtcagaa
      181 tcactttcag ggcgttgcat aactatgcac gaaagcgatt cgcaaacagt ttcgaccgtt
      241 caaaagggcg aacgagtcaa aaaagtaact tttgtgataa ctacttctag atttatggat
      301 aaagtaaggc aatattataa acatttaact aacgaggatt ttataaatga aaaaaataga
      361 tatggaattc tatttaagga attttatcct tgccttatcc ttttacattt tatcttttat
      421 catcgcaagt ttgaatctaa gcccaacaag tatagaatta tttatattta tacttatatc
      481 tctttttgga tcgtccacat accctttttg tctattacta ataaataagc aagcaccctt
      541 actaacaaaa acaactttta acttcgttaa ctggggtttt ataatatgca ttatattatc
      601 actacctcca tttggattga tttttacagt ctattacttt ataaccaaca aaaagcgata
      661 gaaaaacaaa ataaaggtca taccatatca aacggtacga ccttttgttt attaaatgcc
      721 gttaataaag ctgtttagtt gtttcagcct ttcatctgta attaaggcac tagtcactgc
      781 cataattact gcaaacccta aaataccaat aggatttact gccatagcac taaatgccac
      841 tgtaaccata acagatgcta ccttgccgac tgcgattttt tccccgctta taaaagcctc
      901 attccagtca ccagttctga atcctttaac taagccactg ataaatcccc cccactgaat
      961 agcctcacct acgacaccaa aggccttacc aaaaattttt aactgtttag ccatttgatc
     1021 tttattaaga gactccagtg cttttgcaat cgcttctcgg tcggatacgc tgaatttctt
     1081 attaagcaca tccttgtacc tatcaaaggc tttaatcgcc tcattagcat tgcgaatttt
     1141 ttttccttta gcttcatcgg ctagtgcctt ggcttctttc tccaacttat cgccgtattt
     1201 tgatgctaac tctttataaa agtcattaac tttgcttatt gcatcattta cagcattagc
     1261 ttcattttct gccttgaatt ctaaaaagct ttttatagta tcacgagtca gtttattacc
     1321 tgatgacggc ccactaccga tattatagtg aacattcatt ttattcacat caccgtttgg
     1381 tacagttact gtagctactg caccaccagt ataatggata gtgtacgttc cagaatggtc
     1441 atctattgac tgaataagtc ccttaattaa cttatcttta tttgtcggta aatatctgcc
     1501 agttttccca gagtcagggc tccatccagg aatagacgtt gtttttccat tgccgcctga
     1561 attgctgtta tgatttccag caccgtgacc attacctgag ccaccattac cgtgatgttt
     1621 tcctggagga tctttttcca taccaccggt aatgttgata tgcacctccc cgtcaggacc
     1681 tacagtgctt gcaggacctt ttcccggatt gtaatgatac ccaccgccag atgatggtcc
     1741 tccatgtcca gatgagccat tcacgtttcc actgggttta ccaccgttat gtccaagccc
     1801 tgtattacca gggccacgac catctccacc actcataaaa attccctctt ttaatcgtta
     1861 aaacaattaa ttacatagtt aatactgtac ataaaaccac tggttttatg tacagatcaa
     1921 aaagtactcg ccattatttt ttctgtcaag tcagggaggg atattttttg gcagttcgga
     1981 ggaatcactc atgaaaacgg tgaacggaac gcgaaatcct ttcgctgtcc gtgaggcgtt
     2041 ggacaagcga agcgcgtcag tttttccggt tcttacccgt gacaggtttt tccggcagtt
     2101 tatcttccag tatcacaact ttctgataca ccgccgctgt ggcgttgtag ttcctgtcga
     2161 gcattgtttt cattccccat ccgagcacac agaacataac tagaaaagcc actgacaccc
     2221 cacccagcca tttccagcta taccctttaa gggtgttgta ttcctgcacg atattgctgt
     2281 gaattgtcgc aatatccctg ttcagggcat ccgcgtttgc cttcatgttt gcgactgact
     2341 cttccagccg ctgaaaatcc tggctgatgc gctcacctat cagccctgct acgtccagat
     2401 tctgcacctc atttctgact tcatccctga cctgttttgc cacaccgtca gaaatatccc
     2461 gatatccgcg aagtgtttcc cttgccacag ccagagaatc agcaacagct ttctgttgct
     2521 ccattgcggt atgttgcaaa tgctccaccg ataacaggag cgtgagtaat ttttcttctg
     2581 tgctggccat gttcagaatc ccatagaaaa tccacgtgac ggtgatttaa cccgttcagg
     2641 ttcagttcgg tcctgtctgc tcactctgtc tctcatgtca aagccatgct cccgcgcaac
     2701 atcatgttgc agccttctga ccagttcatg ttgcttaacc ggagtgtcca gccattccat
     2761 gatgtcatgc ccgaccgttc gcgcctgttc aagatggcgg gaatagtccg gttcagaccg
     2821 tttttgcatg atgtcatgac gctgggcatc atcaagccgt ccggcagctt caaaacccag
     2881 atgcacttcg ggacgtcgtt caatgccctg ttctttcagc gtccgacagt caatgaagcc
     2941 cctttcaaag ccattttttc tcatatgttc gttgtgcttc acctcccagc gttttcgcaa
     3001 ttcccgcaac tgctcttttt gctgagtggg agtcaggctt cctgaatcct ttttcgcacc
     3061 tccccgctcg ggatacctgg cgttatagcg ccggaaatac tgttccggtg tacgttcaat
     3121 gccatcattc acacgctgac tcatcatgat atgcgcatga ggctgttcgc ctccctcaat
     3181 gctcgctttc ggattgtgaa tggcaaaact ccatgcatgc cgttcgccag cttcctgacg
     3241 tacaaaatcc tgcaccagtt caaggcgctg ttccggtgtc agttcacggg gcagcgctat
     3301 ctccagttcc cgataagtcg aaccgtttgc acgttcaaac tggtcagccg cctgccagaa
     3361 atgggaggga ttatctcgcg cccattccgg catgtttccg tgtgcggaat attccaaatc
     3421 ttgacggtcg cggtatttct ctttgcgctc gatgtaatcg gcatggtttg cagcctgtcc
     3481 cttgccacca aacttaacgc tgaggtgata ggtcgccacg gtttcagccc tccgcaggag
     3541 cgcactgttt cttgacgaag gagagaaaca tataagtgcg cttccttcta attttacgaa
     3601 ttagcacgga agataccccc tgcacccgcc gaccgcaagc gatatactgc acgaccacaa
     3661 cacaccgcaa aaaaacacca gggagcacca cgaacatgcg catcagcaac atcgaatggc
     3721 tgaagaaacg gatcggattc atcaggaagc tcggagaaca gaccgcacga cagagacaga
     3781 ttattgacct gctcgacaac gaagcaggac tcacagaaca ggaacgcaaa ctcctccacg
     3841 tactggcaac agcggaaaaa aacgacttac aggcacagga gagcgaacga aaacaggcgg
     3901 tccaaaaacg aatcgaggga aaaaaacaac gacgagaacg caatcaccga ctctttctcg
     3961 cggctgggct actgatcgaa gcaggactgg tagacacaaa aacaggcgag ctatgctata
     4021 aaaaagacag gatattacag gcacttaagg aaattaagta cgatctggag acatccccaa
     4081 acccagacgc gtaagtgcct ccatccgtgt tccggttccc tggtgcttcc tcgctcattc
     4141 gctcgctacg ctcggtcatg agactgcggc gagcacgacc acctcaatca tgcggtaagc
     4201 cgcaccattg ctgggctgag aggagaaaac cgccggaaag cccgctgctc aaaaagacgc
     4261 cgaaaggcgc tgttatctga tataggcttg ttcatgtccg cgaatttaac atcccattaa
     4321 caaaaaacgg ggggtagtgt tgcttttttg ttaactaaca tttaattatt tgtttcttgg
     4381 ttagccattc tccagaatta tccatccagc gtcgaatagt ccggctgcta acaccaaccg
     4441 ccactgcaat atcttccgtt gacgctccca agatatacat atcaagcgcc ctacgacgtt
     4501 tgtcgtcgtt agcagtccac cgaacagcgg cgcttttttt ccctttgtcc gactgatttt
     4561 ctgacgaatt ggccttcccg cccttgcgac cgcgtgctgc ctgaatttct ggcgtgtgcg
     4621 tatcagccac atactgtgcg aaagtttccg gcgtgaagtt cctgtgcgtg tatttcgcaa
     4681 tgctcttgcc aatagcccga cattcagcca gtgaaagcgg aacgggaagc gatgcattgt
     4741 acatttcgac acgctggatc accgcttcaa gccactgtga gaacgctggc cagccctgac
     4801 gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag gtggcagttt cgccccatcc
     4861 cgtaatgttt atcgatgcta cgacgctctg aggcgctcaa atcgagataa tctgccagtt
     4921 catcgagggt ataggcgtcc tcgcgccact ccatcacctg ccattccagg tggaacggat
     4981 ttttgcaaat caggccgctg tagttcacat cagcacaaag tttctcacac agcgaacgtt
     5041 caaccgctgc ggcatatttc agcgccttaa ccgaggcatc aggcgcggtt ctcacggcga
     5101 tgttgagtgc gtagagcagg tgagcgtgtc cgttcacagg atttttaacg gtgatgttcg
     5161 gcgcgggggc gttccggtcg ctccagtcaa tcgcagcccc ctcgcggtca acgtcaaaaa
     5221 ccagccagaa ctgcgcatga ggctggttct gctggatgta ccgcgcaaga agcgcacgcc
     5281 ctttgccgga gatgcgaagg ccgaaagcga gatcatccgt gtgatagggg cggtggggta
     5341 aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc caggttatcg
     5401 accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa tttcaggggc
     5461 gtt
//
