LOCUS       NZ_CP069953            30896 bp    DNA     circular CON 02-APR-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_1363 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP069953
VERSION     NZ_CP069953.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357505
            Assembly: GCF_016903555.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30896)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30896)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1898.18x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016903555.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:37:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,337
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 32 of 62
            Pseudo Genes (incomplete)         :: 38 of 62
            Pseudo Genes (internal stop)      :: 21 of 62
            Pseudo Genes (multiple problems)  :: 23 of 62
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069953.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..30896
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1363"
                     /culture_collection="FDA:FDAARGOS_1363"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: MD"
                     /collected_by="FDA-Stanford Microbiome Effort"
     gene            join(30817..30896,1..325)
                     /gene="bleO"
                     /locus_tag="I6K93_RS11860"
                     /old_locus_tag="I6K93_11860"
     CDS             join(30817..30896,1..325)
                     /gene="bleO"
                     /locus_tag="I6K93_RS11860"
                     /old_locus_tag="I6K93_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein"
                     /protein_id="WP_001242578.1"
                     /translation="MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEV
                     RIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNT
                     SLKDQWWDERDFAVIDPDNNLISFFQQIKS"
                     /name="bleO"
     gene            832..2094
                     /gene="mobV"
                     /locus_tag="I6K93_RS11865"
                     /old_locus_tag="I6K93_11865"
     CDS             832..2094
                     /gene="mobV"
                     /locus_tag="I6K93_RS11865"
                     /old_locus_tag="I6K93_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            complement(2518..3192)
                     /locus_tag="I6K93_RS11875"
                     /old_locus_tag="I6K93_11875"
     CDS             complement(2518..3192)
                     /locus_tag="I6K93_RS11875"
                     /old_locus_tag="I6K93_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            complement(3320..3505)
                     /locus_tag="I6K93_RS11880"
                     /old_locus_tag="I6K93_11880"
     CDS             complement(3320..3505)
                     /locus_tag="I6K93_RS11880"
                     /old_locus_tag="I6K93_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3510..3848)
                     /locus_tag="I6K93_RS11885"
                     /old_locus_tag="I6K93_11885"
     CDS             complement(3510..3848)
                     /locus_tag="I6K93_RS11885"
                     /old_locus_tag="I6K93_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            complement(4094..4702)
                     /locus_tag="I6K93_RS11890"
                     /old_locus_tag="I6K93_11890"
     CDS             complement(4094..4702)
                     /locus_tag="I6K93_RS11890"
                     /old_locus_tag="I6K93_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            complement(4828..5406)
                     /locus_tag="I6K93_RS11895"
                     /old_locus_tag="I6K93_11895"
     CDS             complement(4828..5406)
                     /locus_tag="I6K93_RS11895"
                     /old_locus_tag="I6K93_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690625.1"
                     /translation="MKIGYARVSTGLQNLNLQEDQLNQYGCEKIFSDHISGSKSKRPGL
                     DKAIEFARAGDTIVVWRLDRLGRNMEDLITLVNELNNRGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNGTPI
                     KTIAEQWKVSRTTIYRYLNRLNNQENKDK"
                     /name="recombinase family protein"
     gene            complement(5670..6050)
                     /gene="blaI"
                     /locus_tag="I6K93_RS11900"
                     /old_locus_tag="I6K93_11900"
     ARG             complement(5674..6050)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(6040..7797)
                     /gene="blaR1"
                     /locus_tag="I6K93_RS11905"
                     /old_locus_tag="I6K93_11905"
     ARG             complement(6044..7797)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7904..8749
                     /gene="blaZ"
                     /locus_tag="I6K93_RS11910"
                     /old_locus_tag="I6K93_11910"
     ARG             7905..8746
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8816..9490
                     /locus_tag="I6K93_RS11915"
                     /old_locus_tag="I6K93_11915"
     CDS             8816..9490
                     /locus_tag="I6K93_RS11915"
                     /old_locus_tag="I6K93_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            complement(<9550..9909)
                     /locus_tag="I6K93_RS11920"
                     /old_locus_tag="I6K93_11920"
                     /pseudo=""
     CDS             complement(<9550..9909)
                     /locus_tag="I6K93_RS11920"
                     /old_locus_tag="I6K93_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(10096..11475)
                     /gene="tet(K)"
                     /locus_tag="I6K93_RS11925"
                     /old_locus_tag="I6K93_11925"
     ARG             complement(10100..11475)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(11637..12491)
                     /locus_tag="I6K93_RS11930"
                     /old_locus_tag="I6K93_11930"
     CDS             complement(11637..12491)
                     /locus_tag="I6K93_RS11930"
                     /old_locus_tag="I6K93_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829558.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_070518910.1"
                     /translation="MDAHTVCISDPKLSFDAMTIVGNLNRDNAQALSKFMSVEPQIRLW
                     DILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRIEFNPNKLTRDEMIWLKQNIISYME
                     DDGFTRLDLAFDFEDDLSDYYAMSDKAVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNK
                     KQERKDNADAEVMSEHLWRVEIELKRDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVF
                     MLLSDEEEWGKLHRNSRTKYKNLIKEISPVDLTDLMKSTLKANEKQLQKQIDFWQHEFK
                     FWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(12803..>13690)
                     /locus_tag="I6K93_RS11935"
                     /old_locus_tag="I6K93_11935"
                     /pseudo=""
     CDS             complement(12803..>13690)
                     /locus_tag="I6K93_RS11935"
                     /old_locus_tag="I6K93_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(13750..14424)
                     /locus_tag="I6K93_RS11940"
                     /old_locus_tag="I6K93_11940"
     CDS             complement(13750..14424)
                     /locus_tag="I6K93_RS11940"
                     /old_locus_tag="I6K93_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            14541..16085
                     /locus_tag="I6K93_RS11945"
                     /old_locus_tag="I6K93_11945"
     CDS             14541..16085
                     /locus_tag="I6K93_RS11945"
                     /old_locus_tag="I6K93_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="WP_000622776.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            16290..16850
                     /locus_tag="I6K93_RS11950"
                     /old_locus_tag="I6K93_11950"
     CDS             16290..16850
                     /locus_tag="I6K93_RS11950"
                     /old_locus_tag="I6K93_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(17173..17910)
                     /locus_tag="I6K93_RS11960"
                     /old_locus_tag="I6K93_11960"
     CDS             complement(17173..17910)
                     /locus_tag="I6K93_RS11960"
                     /old_locus_tag="I6K93_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_011251343.1"
                     /translation="MKSVKEISEELEVSKQTVFNNIKRLNIETVKQDNTSFIKNDVDVE
                     KIIQRVIRNKKKYGFESTNISSEKVKTDNKKNESKYDVQIIELLEKQIDTLNKQLEKQE
                     KRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLESQLEEERQLNYSFDTATNNR
                     QNIDPQEATFTEESQNINQQQTKCQPTEVNYKDIAEEKENDSTNEGELIKEDKFVEETK
                     SDPDDNKEVEPPKKGFWSRFFGN"
                     /name="DUF536 domain-containing protein"
     gene            18296..19252
                     /locus_tag="I6K93_RS11965"
                     /old_locus_tag="I6K93_11965"
     CDS             18296..19252
                     /locus_tag="I6K93_RS11965"
                     /old_locus_tag="I6K93_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_204116041.1"
                     /translation="MSRKNIKNQASQNFYMLHKALFVNEKYKKLSDSAKVTYAILNDRV
                     SLSIKNNWVDDNGDIYFIFTNESLQNILDKSKNTITKIKKELQAVGLLEQIRTGFNKPN
                     KLYLHDIETNISVEKSIQSSSITYNDKESQNLGLQNPEIWDSRIPKFGTPESQILDPND
                     TDYNDTDYIKTESNDTDDLNDKKLTYPNNHTNHSNHDNSNFNNEALKFQLLEELPQSIQ
                     NYLSNFEVTEIEIIKTVLLKAKTSFNNTIDSYYLLEDMEIEILHVLKRFKAILIQKNET
                     VEAMQGYLMKSLKSEFAEMHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(19710..20384)
                     /locus_tag="I6K93_RS11970"
                     /old_locus_tag="I6K93_11970"
                     /pseudo=""
     CDS             complement(19710..20384)
                     /locus_tag="I6K93_RS11970"
                     /old_locus_tag="I6K93_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(20891..21415)
                     /locus_tag="I6K93_RS11975"
                     /old_locus_tag="I6K93_11975"
     CDS             complement(20891..21415)
                     /locus_tag="I6K93_RS11975"
                     /old_locus_tag="I6K93_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002475811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_012818146.1"
                     /translation="MALNFNQATEKTQKRFISVAKHYVERINNDNLKQDFQSAIQQELE
                     DVKSQTQNLVKEFQNHQSDLQKAHDDYKKTLDERIATNETSIKQYDQAFNRLTKGITAM
                     FFIVALVMITFVVLSPLGDLLGVQHFYDWINHVLKTGHSPWRYLIVMFYFVPYILFSLL
                     IYAIFKAYERL"
                     /name="mobilization protein"
     gene            complement(21399..22406)
                     /locus_tag="I6K93_RS11980"
                     /old_locus_tag="I6K93_11980"
     CDS             complement(21399..22406)
                     /locus_tag="I6K93_RS11980"
                     /old_locus_tag="I6K93_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_031906634.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKATRE
                     LYGKTGGNEGHVIIQSFKPGEVTPEQCNQLGLALAEKFATNHQVAVYTHCDTDHIHNHI
                     VINAVNLETGKKFNNNKQALHDLRNFNDDVCREHGLSVPEKDTARLRYTQTEKAIADPN
                     TKSTAQYSWKDDIREAIDQSTATNMDEFKDHLSQYGIEIARVTPKSITYRHLAEDKKVR
                     GRRLGEDYNKGGIEDGFERQIQRQQQAREDDNDIQPKRPTSNRDEPTQRDTGVTQSDWD
                     KFAQDTNELERRRQAAESARLADEKARRDREERERAEQASRTIIDNDRDYGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(22388..22771)
                     /gene="mobC"
                     /locus_tag="I6K93_RS11985"
                     /old_locus_tag="I6K93_11985"
     CDS             complement(22388..22771)
                     /gene="mobC"
                     /locus_tag="I6K93_RS11985"
                     /old_locus_tag="I6K93_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_031906635.1"
                     /translation="MSEQNHFVASDETVGQNHKPNRKEPKQISFRVSESEYLKLKQSAE
                     TLNMSVPAFVKKKAQGSRLVAPKFDKETRQSIAKDLSRLGANANQIAKYCHQHQYDAPN
                     YSALERNISELRERLDDIWQQLN"
                     /name="mobC"
     gene            complement(23396..23938)
                     /locus_tag="I6K93_RS11990"
                     /old_locus_tag="I6K93_11990"
     CDS             complement(23396..23938)
                     /locus_tag="I6K93_RS11990"
                     /old_locus_tag="I6K93_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_011251335.1"
                     /translation="MKYGYIRPVTINDDLNEQKKKIETHTNEIVEEIHANNKKRNILDV
                     LLNEKLVSGDMLYVTDLCILADSTKHLVDILDYLSQEDISLYVINLNRYIYKDIKGEFI
                     EILHDITDFQSDIVKFRTRLGLENYAKEGKNLGRPKRNDKNLSDAIEMYMSKKYTLDEI
                     KEQTNISRATLYRHLDK"
                     /name="recombinase family protein"
     gene            24052..25503
                     /locus_tag="I6K93_RS11995"
                     /old_locus_tag="I6K93_11995"
     CDS             24052..25503
                     /locus_tag="I6K93_RS11995"
                     /old_locus_tag="I6K93_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229532.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_000229535.1"
                     /translation="MVEKLKNEWLNQPGKNILAGIVVALALIPEAIAFSIIAGVDPMVG
                     LYTSFIIAVVTAIVGGRAAMISGATGAIALLIVPLVKDYGVEYLLAATILMGLIQLILG
                     LLKVGRLMKFIPRSVMIGFVNALGIMIFMSQIEHIFGISISTYIYVIVTLLIVYIIPRF
                     FKAIPAPLIAIIVLTALYMYTGSDVRTVGDLGNIKQAFPHFLIPNVPFNLETLQIIFPY
                     SLSMAIVGLVESLLTAKIVDDATDTYSSKNRESCGQGIANMITGLFGGMGGCAMIGQSV
                     INVKSGANSRLSTFTAGIVLIFMIIVLGGLVVQVPMPILAGIMVMVSVGTFDWNSFKYI
                     KKAPKTDAVVMLITVIIVLMTHNLALGVVAGVVFSALFFATKISKVEVISEEFGKINRL
                     SFKGQIFFVSIDSMMEQINFEIENSTIELNFNNAHLWDDSAVDAIDTIVRKFEEKNNIV
                     RVEKLNSDSRKVVSELSQLNENHLN"
                     /name="SulP family inorganic anion transporter"
     gene            25517..25930
                     /locus_tag="I6K93_RS12000"
                     /old_locus_tag="I6K93_12000"
     CDS             25517..25930
                     /locus_tag="I6K93_RS12000"
                     /old_locus_tag="I6K93_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282781.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_000278297.1"
                     /translation="MYKSILLAADGSENSLRSAKEVLNFIDDNTIVTILTVVDVEESKT
                     DVLHGKQSSSLTNEREDKLSHITELFVEHNVKYEMKIAHGVPADTVVSVANSGEFQAIV
                     LGTRGLNSLQEMVLGSVSHKVAKRSEIPVVIVK"
                     /name="universal stress protein"
     gene            25986..26429
                     /locus_tag="I6K93_RS12005"
                     /old_locus_tag="I6K93_12005"
     CDS             25986..26429
                     /locus_tag="I6K93_RS12005"
                     /old_locus_tag="I6K93_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012817960.1"
                     /translation="MKTKNFYYGWVIVFIAGLTVFFSGPGQTYSNAAFIDEYIQTFGWS
                     RTEVSSIYSLAILFGIIWGIANGLERIGTNIVWANYFGRKHVGSINGIESTMLVIGSSL
                     GALPLGLGHDIFQSYSLTIYMMIIFPIIGLVCALLAKKPVKNY"
                     /name="hypothetical protein"
     gene            complement(27225..>28010)
                     /locus_tag="I6K93_RS12010"
                     /old_locus_tag="I6K93_12010"
                     /pseudo=""
     CDS             complement(27225..>28010)
                     /locus_tag="I6K93_RS12010"
                     /old_locus_tag="I6K93_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458740.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(28069..28743)
                     /locus_tag="I6K93_RS12015"
                     /old_locus_tag="I6K93_12015"
     CDS             complement(28069..28743)
                     /locus_tag="I6K93_RS12015"
                     /old_locus_tag="I6K93_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            28810..29670
                     /locus_tag="I6K93_RS12020"
                     /old_locus_tag="I6K93_12020"
     CDS             28810..29670
                     /locus_tag="I6K93_RS12020"
                     /old_locus_tag="I6K93_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_275691666.1"
                     /translation="MHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPM
                     CNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRM
                     MQYKKINKNLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFW
                     KKAMKLDYDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDL
                     EEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKN
                     YFIKE"
                     /name="protein rep"
     gene            29830..30600
                     /gene="aadD1"
                     /locus_tag="I6K93_RS12025"
                     /old_locus_tag="I6K93_12025"
     ARG             29831..30597
                     /name="aadD1"
                     /gene="aadD1"
                     /gene_name="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             14542..16082
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             29831..30600
                     /name="ANT(4')-Ia"
                     /gene="ANT(4')-Ia"
                     /gene_name="ANT(4')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.21"
                     /coverage="101.19"
     ARG             7905..8749
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="95.02"
                     /coverage="100.0"
     Typing          22743..22979
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.16"
                     /coverage="100.0"
                     /evalue="7.55e-118"
     Typing          complement(11638..12503)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          18219..19252
                     /name="rep_cluster_910"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(27226..28007)
                     /name="rep_cluster_1442"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.614"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          28801..29670
                     /name="rep_cluster_1018"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          833..2091
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9539..9909)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="100.0"
                     /evalue="1.1e-70"
     Typing          complement(12807..13690)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.21e-157"
     Typing          complement(21403..22367)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.963"
                     /coverage="95.0"
                     /evalue="6.66e-177"
ORIGIN
        1 tttcactttg gttcaccatg aagatggatt cgcagttcta atgtgtaatg aggttcggat
       61 tcatctatgg gaggcaagtg atgaaggctg gcgctctcgt agtaatgatt caccggtttg
      121 tacaggtgcg gagtcgttta ttgctggtac tgctagttgc cgcattgaag tagagggaat
      181 tgatgaatta tatcaacata ttaagccttt gggcattttg caccccaata catcattaaa
      241 agatcagtgg tgggatgaac gagactttgc agtaattgat cccgacaaca atttgattag
      301 cttttttcaa caaataaaaa gctaaaatct attattaatc tgttcagcaa tcgggcgcga
      361 ttgctgaata aaagatacga gagacctctc ttgtatcttt tttattttga gtggttttgt
      421 ccgttacact agaaaaccga aagacaataa aaattttatt cttgctgagt ctggctttcg
      481 gtaagctaga caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg gagatttttt
      541 gagtgatctt ctcaaaaaat actacctgtc ccttgctgat ttttaaacga gcacgagagc
      601 aaaacccccc tttgctgagg tggcagaggg caggtttttt tgtttctttt ttctcgtaaa
      661 aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca ctgccgacag cctcgcagag
      721 cacacacttt atgaatataa agtatagtgt gttatacttt acttggaagt ggttgccgga
      781 aagagcgaaa atgcctcaca tttgtgccac ctaaaaagga gcgatttaca tatgagttat
      841 gcagtttgta gaatgcaaaa agtgaaatca gctggactaa aaggcatgca atttcataat
      901 caaagagagc gaaaaagtag aacgaatgat gatattgacc atgagcgaac acgtgaaaat
      961 tatgatttga aaaatgataa aaatattgat tacaacgaac gtgtcaaaga aattattgaa
     1021 tcacaaaaaa caggtacaag aaaaacgagg aaagatgctg ttcttgtaaa tgagttgcta
     1081 gtaacatctg accgagattt ttttgagcaa ctggatccag gagaacaaaa acgatttttt
     1141 gaggaaagtt ataaattatt ttccgaacga tatggcaagc aaaatattgc ttatgcaaca
     1201 gttcataatg atgagcaaac ccctcacatg catttaggtg ttgtgcctat gcgtgatgga
     1261 aaactgcaag gaaaaaatgt gtttaatcgt caagaactgt tatggctaca agataaattc
     1321 cccgagcata tgaaaaaaca gggttttgag ttgaagcgtg gtgaacgtgg ctctgaccgt
     1381 aaacatattg agacagctaa atttaaaaaa caaactttgg aaaaagagat tgattttcta
     1441 gaaaaaaatt tagcagttaa aaaagatgaa tggactgctt atagcgataa agttaaatca
     1501 gatttagaag taccagcgaa acgacacatg aaaagtgttg aagtgccaac gggtgaaaag
     1561 tccatgtttg gtttgggaaa agaaataatg aaaacagaaa agaaaccaac caaaaatgtt
     1621 gttatatcgg agcgtgatta taaaaactta gtgactgctg cgagagataa cgataggtta
     1681 aaacagcatg ttagaaatct catgagtact gatatggcga gagaatataa aaaattaagt
     1741 aaagaacatg ggcaagttaa agaaaaatat agtggtcttg tagagcgatt taatgaaaat
     1801 gtaaatgatt ataatgagtt gcttgaagaa aacaagtctt taaagtctaa aataagcgat
     1861 ttaaagcgtg atgtgagttt aatctatgaa agcactaagg aattccttaa ggaacgtaca
     1921 gacggcttaa aagcctttaa aaacgttttt aaggggtttg tagacaaggt aaaggataaa
     1981 acagcacaat tccaagaaaa acacgattta gaacctaaaa agaacgaatt tgaactaact
     2041 cataaccgag aggtaaaaaa agaacgaagt cgagatcagg gaatgagttt ataaaataaa
     2101 aaaagcacct gaaaaggtgt ctttttttga tggttttgaa cttgttcttt cttatcttga
     2161 tacatataga aataacgtca tttttatttt agttgctgaa aggtgcgttg aagtgttggt
     2221 atgtatgtgt tttaaagtat tgaaaaccct taaaattggt tgcacagaaa aaccccatct
     2281 gttaaagtta taagtgacta aacaaataac taaatagatg ggggtttctt ttaatattat
     2341 gtgtcctaat agtagcattt attcagatga aaaatcaagg gttttagtgg acaagacaaa
     2401 aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct aatgttgatt actttgaacg
     2461 gttctgttgc aaagtaaaaa atatagctaa tcactaatat atcatgtcag tgttcgttta
     2521 acttgctagc atgatgctaa tttcgtggca tggggaaaat ccgtagatct gaagagacct
     2581 acggttcttt ttatatagag cgtaaataca ttcaatacct tttaaagtat tctttgccgt
     2641 attgatactt tgatatcttg tctttcttac tttaatatga cggtgatctt gctcaatgag
     2701 gttattcaga tatttcgatg tacaatgaca gtcaggttta agtttaaaag ctttaattat
     2761 tttagccatt gctgccttcg ttgaaggtgc ctgatctgta actacctttt gaggcttacc
     2821 aaattgttta atgagacgtt tgataaacgc atatgctgaa tgattatctc gttgcttacg
     2881 caaccaaata tctaatgtat gtccctctgt atcaatggca cgatataaat agctccattt
     2941 tccttttatt ttgatatacg tctcatcaat acgccatttg taataagctt ttttatgctt
     3001 tttcttccaa atttgatata aaattggggc atattcttga acccaacggt agatcgttga
     3061 atgatgaacg tttacaccac gttctcttaa tatttcagat atatcacgat agctcaatgc
     3121 atatcttaga tagtagccaa cggctacagt gataacatcc ttgttaaatt gtttatatct
     3181 gaaatagttc atacagaaga ctcctttttg ttaaaattat attataaatt caactttgca
     3241 acagaaccca aaatttaata gtactactac acaaaaagag agtgcgaatt cgcactctct
     3301 ttttgtgata aatgaatggt taagaaaata attcatccac agttgtatca aacaatcttg
     3361 ccaacttaaa tgctaatgtc aatgtaggat catatttatt gttttctatt gcattaatag
     3421 tttgcctaga cacttttaat tctttactta aagtttcttg agaaattcct ttttgttttc
     3481 tatattctct aattctattt ttcattaatt taacctttag atgtatattt tttatttgta
     3541 gtatagtaat taaacagcat aagaaatagt gctacaatta taaagaaaat ttctggatat
     3601 tgagaaaacc aagatccttc aatacttggt gacaaacctg taaatttaag cactagtgta
     3661 aataacatac aatatacaat ccaaataaaa cttttagcca gtatttcaat aaacatttta
     3721 cgttgtcttt catcgtatcg atcttgttgt ccttttttat ctaatgtttc tgaaggtaaa
     3781 tatttgttag ttatagccgt ccataaacaa gaaaaaatta aaataataat aaaaggaatt
     3841 aaattcatta taacacctca taaatgtaaa aagtatttga catttatgag tgtaaatatt
     3901 tttgatacaa atgtcaaata ctttttacat tataatatat tttaaaacac cttattagta
     3961 aaacaattac tatatagaaa gctgcgaact cgtactcttt tttcttgtat aattattaaa
     4021 atctcaattc aataatatgt tatggaattt gagacactgt gagagacgct catttgcatt
     4081 gacaatcaat ttatcaagat aatcccttat catgtttaat tctataaact gtttgtcttg
     4141 tgatattaac ttctttcgca atcttactaa tcgctttgcc ttcttctaac atttccacca
     4201 cgcgatgata aataacacgt ttctgtgggt ctttagcatt tggtgaataa agtaaagggc
     4261 gccctttata tacgcctttt tccttcgcaa cttgaatccc ttgcgcttgt cgacgtttac
     4321 tttcatttct ctcttgttct gaaaccattg ctaatatttg aatgattaaa tctttcataa
     4381 acttatccag taatggattg ccaattactt cattcatcat aggtaagctg gtaatcatta
     4441 actgaacgtc tttttctttt aaataattca cagtttcaat aatttcatca tagttacgtc
     4501 ctaaacgatc aatagattcc acaacgaatc gatctcccat tctgacaaaa ttgagtgctt
     4561 cttgaaatac aggtctattt tccactgact ttcccgactg tttctctgta aagattttct
     4621 ccacaccaaa cgtctttaaa ttatccaatt gtctttctaa attttgatct atcgatgata
     4681 ctctcgcata acctataatc attcatcttc actccataat aaatatacac cctaatcata
     4741 cgttttaatt gttataattt caacaattct ttttgtatgg ttagggtgtg tcctgtctat
     4801 ttttatcttt cttaaaactt agtaatatta tttatcttta ttttcttgat tatttaacct
     4861 attgagataa cgatagattg tggtacgtga aactttccat tgttctgcaa tagttttaat
     4921 tggtgttcca ttatcataaa gtgttttgag taactttaaa tcttgtttat ttaatttttc
     4981 gggacgacct ccatagcgtc ctctagcacg agcggcaatt cgtcctgctg aagaacgttc
     5041 taaaattaag ttgcgttcaa actctgcaaa tgctgcgaat aaatgaaata acaattgtcc
     5101 tgtagaactt gatttatcca ttgttatatt ttcttctaaa ctatgaaaac tcacgcctcg
     5161 attattaagt tcattaacta atgtgattaa atcttccata ttacgcccta gtctatctag
     5221 tctccaaaca acaatcgtat ctcctgccct cgcaaattca atggctttat ctaaaccagg
     5281 acgtttactt tttgatccac ttatgtggtc gctaaatatc ttttcacaac catattgatt
     5341 taattgatct tcctgcaaat ttaaattttg taaaccagtt gaaactcttg cataacctat
     5401 tttcaaaata caaacctcct atatacatct tcattgtatc ataacttaat tctaaccata
     5461 tttatgaaca tagattttct gttgggttat tgaacactaa aatatgtata ctcgctatat
     5521 atatagacat aaaaagagag tgtatagaaa atggttattt tctggacact ctcatattta
     5581 ataaaatatt ttaaatgtta ttaacaaaac taatttaata agagtcaagc atagtttaca
     5641 tatgtattat gtttgaaaca tatctctaat tactttttac taatatcatt taaaatgtct
     5701 cgcaattctt caatttcttt gttatttaat tcttcatttt tcgcaaaatt cagcactaaa
     5761 cttttcatgt cccctccata cagtttatta agaaaggttt tagcagtttt cattttaata
     5821 tcgtcttctt taatatttga tgagtaaaaa taaatattct ctgatttgta tcgttttata
     5881 atctcttttt tatatagtct tgtgattaat gttctaatcg ttttatcgct aacttcttta
     5941 tatttttgaa tttcaactac aatttcatta gctgatactg attttttatc ccatattata
     6001 ttcataacat cccattcagc catagatatt tcaacttgct tattggccat ttaaaacacc
     6061 catttctttt aatatttttt cactgattaa ttcagcattt ttcccagacg gctttccatc
     6121 tgataaatgt gtagcaaaat aatacttatc atgatttgta attacgtaac ctacaaacca
     6181 cccattatta tacttcccgt ttactatacc tgtacctgtt ttcccataca gttcatactt
     6241 ttcatttttc ttaatcaata atgaagaaga taattgattc tttgcttttt tactaaaatg
     6301 gttattttgt tccatcatat ttttaaaaac tattacttgt tcaagattag atattttcaa
     6361 actatcttcc atccaatagc ttttataact tcccaagttt tcattaccat aatttaattg
     6421 cttgagttga gccgcagtat agttctttgg tatttgatta ctaatacgtt cgaagtacca
     6481 attaactgaa ttttgcattg ctgtatttaa atcttgttcc ttattccaag actcgaaagg
     6541 ataatgttta tgattccatg acatacgtga atttttatca cttatgatat gatggtcaag
     6601 tccaaacatc gctaaataaa ttttataagt tgaatcagga gaatatctct ttctactttc
     6661 tttttcatta taaatatagt atttgtcttt cttcatgctg tacatgacga aagatccact
     6721 atttgaacca aaatttttac tttcatctaa aatttggtga tcgttctgta agggcttttt
     6781 ataattataa tcagttatgg attgccccat caaaaactgg ctttgaatta ccatgagcaa
     6841 aaaagtaaaa atacaaataa aaattggaat taatttggac tgttttttca aattggcttc
     6901 ttttatatta attaatcttc ttttgagtaa tgacttttta ccattaaatg aatggcttaa
     6961 tatattttta ttaaaaaatg gaatatttaa tacggagtcc ataatagatt ccgcataagt
     7021 tttaaattca tttttgttaa tattattaag aacaaatcta tcggcttcta cttcattgtc
     7081 atgaattatc tttcttttta caatatgtat taatggattg taactcataa caatagagaa
     7141 gatattaaag ataattaaat gtaaagtatc tctattttta gcatgagcat attcatgcaa
     7201 tattatatat tttagtcttt tgtcaattac acttttaaaa tatgaactag gaatcaaaat
     7261 aatatatttc ccataccaaa aagttattgg agattgaata gtctctgctt ttcgaatcac
     7321 aatatttttt ttatattgat ggttgaaaag tatcgtatct attttatttt tttcattttc
     7381 gtttagataa agtgactgtc tctttaaata tttaagatat aataaggatt tcaaaaattt
     7441 aaaacttaaa ataataacca aaactatcca aataactgtg cagatattat caattgaatc
     7501 ccaattaaac ttatggatat ctgttgtgaa ctcttgaaca ggtttggtgg tatttatgtt
     7561 atggttcaag tcgtgtgacc tactttcaac tgtgggcgat tgattattca cattattaaa
     7621 ttttataaaa gagaatttaa ttgggataaa aggaattaat ccagcaagca cagttagata
     7681 ccaaacttta taatttaaac tataattaaa atagcgtttt aatatatacc taaaaaatac
     7741 taacaataga aatataaaac aaaagcttac tacgctcatt attagtaatt tagccatttt
     7801 gacaccttct ttcaattatt tataataaac aattgacacc aatattacaa ttgtaatatt
     7861 attaatttgt aaaaattaca actgtaatat tggagggttt attttgaaaa agttaatatt
     7921 tttaattgca attgctttag ttttaagtgc atgtaattca aacagttcac atgccaaaga
     7981 gttaaatgat ttagaaaaaa aatataatgc tcatattggt gtttatgctt tagatactaa
     8041 aagtggtaag gaagtaaaat ttaattcaga taagagattt gcctatgctt cgacttcaaa
     8101 agcgataaat agtgctattt tgttagaaca agtaccttat aataagttaa ataaaaaaat
     8161 acatattaac aaagatgata tagttgctta ttctcctatt ttagaaaaat atgtaggaaa
     8221 agatatcact ttaaaagaac ttattgaggc ttcaatggca tatagtgata atacagcaaa
     8281 caataaaatt ataaaagaaa ttggtggaat caaaaaagtt aaacaacgtc taaaagaact
     8341 aggagataaa gtaacaaatc cagttagata tgagatagaa ttaaattact attcaccaaa
     8401 gagcaaaaaa gatacttcaa cacctgctgc tttcggtaag actttaaata aacttatcgc
     8461 aaatggaaaa ttaagcaaag aaaacaaaaa attcttactt gatttaatgt taaataataa
     8521 aagcggagat actttaatta aagacggtgt ttcaaaagac tgtaaggttg ctgataaaag
     8581 tggtcaagca ataacatatg cttctagaaa tgatgttgct tttgtttatc ctaagggcca
     8641 atctgaacct attgttttag tcatttttac gaataaagac aataaaagtg ataagccaaa
     8701 tgataagttg ataagtgaaa ccgccaagag tgtaatgaag gaattttaat attctaaatg
     8761 gttctgttgc aaagttgaat ttataatata attttaacaa aaaggagtct tctgtatgaa
     8821 ctatttcaga tataaacaat ttaacaagga tgttatcact gtagccgttg gctactatct
     8881 aagatatgca ttgagctatc gtgatatatc tgaaatatta agagaacgtg gtgtaaacgt
     8941 tcatcattca acgatctacc gttgggttca agaatatgcc ccaattttat atcaaatttg
     9001 gaagaaaaag cataaaaaag cttattacaa atggcgtatt gatgagacgt atatcaaaat
     9061 aaaaggaaaa tggagctatt tatatcgtgc cattgataca gagggacata cattagatat
     9121 ttggttgcgt aagcaacgag ataatcattc agcatatgcg tttatcaaac gtctcattaa
     9181 acaatttggt aagcctcaaa aggtagttac agatcaggca ccttcaacga aggcagcaat
     9241 ggctaaaata attaaagctt ttaaacttaa acctgactgt cattgtacat cgaaatatct
     9301 gaataacctc attgagcaag atcaccgtca tattaaagta agaaagacaa gatatcaaag
     9361 tatcaatacg gcaaagaata ctttaaaagg tattgaatgt atttacgctc tatataaaaa
     9421 gaaccgtagg tctcttcaga tctacggatt ttccccatgc cacgaaatta gcatcatgct
     9481 agcaagttaa acgaacactg acatgatata ttagtgatta gctatatttt ttactttgca
     9541 acagaaccat aaattatctt taccgtattc ttgttctaaa aactctttag catattcaaa
     9601 aaactgcttt gtatcttctg gcgtttgatt atcaaagaaa tcattgtctg atgtaattaa
     9661 accatcaatg tgtttaatcg cgtctgttct aatttttctt ttgcctgtat aattctgttc
     9721 gattttttca tcaatcaagt tattaaaatt ctgtttatta gcatttacca aatcataatt
     9781 taagtaagtt ttactatggt ctatatcttc attttcataa ttattatttt ctctttgaac
     9841 atgtttttgt atgcccgttg tatttgttcc agatttaact tttgaaactc taacaatgga
     9901 ataagacata taaaaatcac tcctaagcac aaaggtttca tgtgtattta atagtttaag
     9961 tctaacacac tagacttatt ttacaataag tcgttaatac gtgtgctctg cgaggcttaa
    10021 acctgttttt gctaacgcaa aaaatgagtg gcaaaatgct agccactcat agttctaaac
    10081 caaaatataa tataactatt caaactgctt ttcagaacgt ttaaatacaa taatcgtcaa
    10141 aagacaacat aaaataataa ggatagccat ggctacaaga atattactat acactccaga
    10201 agaataattt ataaattcca gaactagttt acgattaatc aattgtagtg acaataaacc
    10261 tcctacaatt gctatacctg ttccctctga taaaaaactt gtgaaattta gcaaactcat
    10321 tccagaagca acttcttctt cagaaagact acttgatact atttttgata taactgtttt
    10381 agtaaaagat aatccgccca taacaaatat aaacataaaa gtagtcaacc acatactaaa
    10441 ctcaacaaaa aatgcaatag ttaaaaaact tatagagata gacaatgatc ctaaaataaa
    10501 aacaaataat gatccttttc tatccactaa aaaaccacca aaataaccaa aaacaataac
    10561 actcatggtt ccaggaaaaa taacactatt acctattgtc gctacattta catgataaat
    10621 agttttcatc atataaggca ccattgatat aaaaccagct actatagaaa atattagccc
    10681 accagaaaac aaaccaagca taaacggaat gtttttccct agtttaggat taataaaagg
    10741 gttagaaact cttgaaatat gtttaataaa aatcacaaaa aagattgtga agagtattaa
    10801 aaaagtccaa ttataatttg tcgtaaataa cataaaacat ataatactta tagacattaa
    10861 aacaatacct acgatatcta atgtattttt tgttgattta ccaggtacca ttactttaat
    10921 aagaaaaggt atagttacta ttgtaatcat aggaagtata agtaggtaag accaatgaat
    10981 ataatgtgct attattcccc ctattgaagg acctaaccct tcacctaaag ctacaattga
    11041 tcctataaaa ccaaaggctt tgccttgttt ttttcttgta atatttctag ctacaaccac
    11101 cataatcagt gaagggaatg cagcagatcc tactccttgt actaacctac caaaaatcaa
    11161 aataaaaaag tgattgtgac caataaaagc aatcaatgaa ccaagacagc tcaaactaat
    11221 accaataatt aacaattttt ttatatttat ataatcagat aattttccat atactgctgt
    11281 tcctatcgaa aaagttaaca tatatgcagt gtttacccag tttgtaattc caggagtagt
    11341 attaaaatga tttgcaatat caggtaaaga aacatttaaa accatttcat ttaatacact
    11401 aaaaaatgaa agaatacaaa gccaaaataa aacgctataa aacaaacctt taaatttttt
    11461 atataaacta aacaatttta cctctttact tgaggtgact aaagtttata ggggtaaatt
    11521 attctttcac ctcatataaa ttccccaaat ttttaaatag acacttcata aaaaaatcct
    11581 cctaaattag aatagtttaa atatatcatt tttgttcagt aatattaata tgtacactat
    11641 ttccaaaatt taaattcatg ttgccaaaaa tcgatttgtt tttgcaattg tttttcgttc
    11701 gcttttaaag tcgatttcat taagtccgtt aaatcgactg gcgaaatttc ttttatcaaa
    11761 ttcttatatt ttgttctaga atttctgtga agctttcccc attcttcttc atcactcaat
    11821 aacataaaaa ctattgctct atccgcagtg cgttggatag ttttccaatc tggttgcaag
    11881 atatgtaaat cactaaagca atcattccag taatccacca tatctctttt aagttcgatt
    11941 tctacacgcc ataaatgttc agacataact tcagcatctg cattatcttt acgttcttgc
    12001 tttttattat aaattctaat aaatctatta ctatctctca cgccaaaata ttttgtttct
    12061 ggcttaccat tacgaccata aaaaatagtt ttcttaactg ctttatcaga cattgcatag
    12121 tagtcactca aatcatcttc aaaatcaaag gctaaatcta atcttgtaaa accgtcatct
    12181 tccatgtagc ttattatatt ttgttttaac caaatcattt catctcgtgt aagtttgttt
    12241 ggattaaatt caatacgcat attacgtcta tcccaactat ctgctttcac tttgtcatat
    12301 tcaatataaa ctttttcttg aagtgcttta gctttaaact ttgtttgaag aatatcccaa
    12361 agtcttattt ggggctctac actcataaat ttagaaaggg cttgagcgtt gtctcggttg
    12421 agatttccaa cgatcgtcat tgcatcaaaa cttaattttg gatcagatat gcacacagta
    12481 tgtgcgtcca accggctatt agatcaagtc tttttttatt taagcatttg tattatctgg
    12541 taaacagtta aaactactaa aacaccaagt acatacttac ttgttataaa attctcaaag
    12601 gcttctctga atttcatttc ttcaactcct tttattgctt tattccaatt tccttattgg
    12661 tcggaaccta cagggcttta tgtgttgttg agttggtact ttcttgggat taatcccaat
    12721 tcaagtccaa ccaactcgct aacaagttag ctaacacata gcccattcca accaataagt
    12781 tttctcggca taaatgcatg ttctataagt gcatacctct atctcgtttt tgattctttt
    12841 gttgttcttg ttgctgttcc tgttctgaat ataattctgg attcattttt ttatctaagt
    12901 ttcctgctaa accatttagt tttggttcat cttctgttaa cttattaact ctttccgcaa
    12961 aggtatttct tcctaaaact cgttctaact cttttagtaa gatttttaaa agctttaacg
    13021 ctatttctat attctcttta agtggctttg cattttcgta aagttggtta aaattatcgt
    13081 ctgcgttttc aatacgctca actgctttat ttaataaatc atctttctct cgtatttcct
    13141 tatctttatc atctaaggct ctaccagact ttatatactc gtaatcttcc gaaatatctt
    13201 gagcagcttt tatctgtttc tgaaattcat tgaaatcttt ttggcttatt acaacatttc
    13261 cagtttcttg tatttctttg ctaaataaac cacctacttt ttcagtttct tgctcatacg
    13321 gaacatttat aggcttttta agcgtattta acgatttttg gtactcttgc attaatttat
    13381 cgttcttttg ctttatatgg tctgtttttt ggctctcacg ttcatattct tgcttatgat
    13441 attctgtttt ttgtttatac tgacttattt gctcatgttt agcatttgtt acttgtcttg
    13501 attgcccacg ttctaaatca tatcctcgtt gtttaacatg ctcattaaat ctatcttgaa
    13561 acgctgttaa agctttttta ttacctacaa cttctttagc acttaaacga ccatcatcag
    13621 ttattggaac aacgccataa tgcatatgtg gtgttttttc gtccatgtga actgttgcat
    13681 ataataaatt aggttctgtt gcaaagtaaa aaatatagct aatcactaat atatcatgtc
    13741 agtgttcgtt taacttgcta gcatgatgct aatttcgtgg catggggaaa atccgtagat
    13801 ctgaagagac ctacggttct ttttatatag agcgtaaata cattcaatac cttttaaagt
    13861 attctttgcc gtattgatac tttgatatct tgtctttctt actttaatat gacggtgatc
    13921 ttgctcaatg aggttattca gatatttcga tgtacaatga cagtcaggtt taagtttaaa
    13981 agctttaatt attttagcca ttgctgcctt cgttgaaggt gcctgatctg taactacctt
    14041 ttgaggctta ccaaattgtt taatgagacg tttgataaac gcatatgctg aatgattatc
    14101 tcgttgctta cgcaaccaaa tatctaatgt atgtccctct gtatcaatgg cacgatataa
    14161 atagctccat tttcctttta ttttgatata cgtctcatca atacgccatt tgtaataagc
    14221 ttttttatgc tttttcttcc aaatttgata taaaattggg gcatattctt gaacccaacg
    14281 gtagatcgtt gaatgatgaa cgtttacacc acgttctctt aatatttcag atatatcacg
    14341 atagctcaat gcatatctta gatagtagcc aacggctaca gtgataacat ccttgttaaa
    14401 ttgtttatat ctgaaatagt tcatacagaa gactcctttt tgttaaaatt atattataaa
    14461 ttcaactttg caacagaacc aaatagatta taatccttat agaccgatcg cacggtctat
    14521 aaggattgga gggaacttaa atgatttcat tttttacaaa aactactgat atgatgacat
    14581 caaaaaaaag atggactgca ctagtagtat tagctgttag tttgtttgtt gttacaatgg
    14641 atatgacaat attaattatg gctttaccgg aattagtaag agagttagag ccttctggta
    14701 cccaacagtt atggatagtt gatatatact ctcttgtttt agctggcttt ataattccat
    14761 tgagtgcctt tgctgataaa tggggaagaa aaaaagcatt attaactgga tttgctttat
    14821 ttggcctcgt ttcattagct atatttttcg cagaaagtgc agagttcgta atagctattc
    14881 gatttttact tggtattgca ggtgctttaa taatgccaac tactctttca atgataagag
    14941 taatttttga aaaccctaaa gaaagggcca ctgcattagc tgtatggtca atcgcttcat
    15001 cgataggtgc tgtttttgga ccaattatcg gaggagcttt acttgagcaa ttttcatggc
    15061 actcggcatt tttaattaat gtaccgtttg cgataatagc agttgtagca ggtttatttt
    15121 tattaccaga gtctaagtta tcaaaagaaa agtctcactc gtgggatatt ccttctacaa
    15181 ttttatcaat tgcaggcatg attggactgg tatggagtat caaagaattt tcaaaagaag
    15241 gactagcaga tattattcca tgggttgtaa tagtattagc aattaccatg atagtgatat
    15301 ttgttaaacg taatttatca agttctgatc caatgttaga cgtaagactt tttaaaaaga
    15361 gatcattttc agctggtaca attgctgcat ttatgacaat gtttgcaatg gcatctgttt
    15421 tgttattagc ttcacaatgg ttacaggttg tggaagaact ttctcctttt aaagctggct
    15481 tatacctatt acctatggca ataggagata tggtgtttgc accaattgca cccggattag
    15541 cggcgcgatt tggaccgaaa atagtgttac cttccggaat tggaattgca gccattggca
    15601 tgtttattat gtatttcttt ggtcatccat tatcatattc tacaatggct ttagcattaa
    15661 ttttagttgg agctggtatg gcttcactag cagttgcatc tgctctaata atgttagaaa
    15721 cacctacatc aaaagcaggt aatgcagctg ctgttgaaga gtctatgtat gaccttggaa
    15781 atgtttttgg tgtagcagta cttggtagcc tatcttctat gctttatcgt gtatttttag
    15841 atatttcatc tttttcatca aaaggtatag ttggagattt agctcatgta gctgaagaat
    15901 ctgtagtggg cgctgtcgaa gtagctaaag ctacggggat aaaacagctt gcaaacgagg
    15961 ctgtaacatc atttaatgat gcttttgtag caactgcttt agtaggtggg attatcatga
    16021 ttatcatttc aatagttgtc tatttgttaa ttcccaaatc acttgatata actaaacaaa
    16081 aatagtacta aacatatatt tttagatatt gtagattagt ttatttttaa aattagtaat
    16141 agttaaaaag tagaaccatt aattttaaat ggttctactt ttttattatt aagtaaaatt
    16201 ataaaagaca atttcttaaa ttaataatat gatataccta tatctaataa tttattagta
    16261 ttaataaacc ttgaaaggat gattaactga tgaatagagc agatattttt aaaatatttg
    16321 atgtagaact taatataact atatttttag gagcactagt agcaactttc atctatttta
    16381 taagttacat gaacaatgta aatgaattaa ttacattatg cttaacatta gcttttcctg
    16441 catatttatt aaaaattagc agatcgttcg aggttatttt attagaggaa aaaataaaaa
    16501 atagtaaaat caaaatctgt gacaaagata gaattgaacg agagatactg gtgaaaaaga
    16561 acctaataga tgaactattg tttcctaata gagttattaa aaaatataca ctgacaattt
    16621 taatttacag tataggtatt attattcttt tactactttg taatatgtta agttatggaa
    16681 ttggtgaaaa tgtcaatttt atctggagat tactagcttg ttatgtaacg agtgtcacag
    16741 tgattatagt tgccttgatt ataatgataa tgatatttga tcttataatt ggattactat
    16801 taaattttca aatattttta aaaaataaaa agtatgatga ctttttataa ttattcgatt
    16861 tatataatga aaaattaaat gattaaacat agaaacctca aaacaaacca tatttaatag
    16921 agtttatggc gccttataca aataaaggct atgctgttaa agaattatgt agctatcttc
    16981 agattaatat ggatgatgtt tatactattg gcgatagaaa attgatttac ctatgtttaa
    17041 taccctagat aattcttctg ataatttagt ttttgttaga gtttaacata actgatgatt
    17101 atcagaagtt ccagcactat aaaaggtgta tcaagggttt ataaaccctt gatacacctt
    17161 ttacattcat ttttaattac cgaagaatcg actccaaaat ccttttttag gtggttctac
    17221 ctccttatta tcgtcaggat cactcttcgt ttcttcaacg aacttatctt cttttattaa
    17281 ttcaccttca tttgtactgt cattctcttt ctcttcggct atatccttgt aatttacttc
    17341 tgttggttga cactttgttt gttgttgatt gatattttgt gattcttcag taaaagttgc
    17401 ttcttgcgga tcaatatttt gtctattatt cgtcgctgta tcaaaagaat aattaagctg
    17461 tctttcttct tctaattggc tttctagttt ttgaatcttt ttattacttt ctaatgctaa
    17521 tacttgttgg ttttcaagta atttcgacct ttcttgtaat tcttttctat aaaattctat
    17581 tgtcgtttca tgacgttttt cttgtttttc aagttgttta tttaaagtat ctatttgctt
    17641 ttcgagtagt tcaattattt gaacatcata tttagattcg ttttttttat tatctgtttt
    17701 aactttctct gaacttatat tagtactttc aaagccatat ttctttttat ttctgattac
    17761 tctttgaatg attttttcta catcaacatc atttttaatg aatgaagtat tatcctgttt
    17821 tactgtttct atattcaatc tttttatatt attaaataca gtctgtttac taacttccaa
    17881 ttcttcactt atttctttaa cgctcttcat atcttcaacc ctttataaac actttatcaa
    17941 ccctttataa ataaatttta tcataaatac ttttataaag ggagcttgag cagtcttaaa
    18001 aaacctttta taaacagttg acaagatcat cgtcaactgt ttataaaagg tttttaatgg
    18061 gattataata tcttttatat gttctttttt aatgatataa tgttcataat acgttttagt
    18121 cccaactact ggtaatagtt gggactgggt aataaaacgt tacaatacgt tagctattta
    18181 atttgatata tgtattatat acaacaatat ctacagtttg caagtcaaaa agttatgtgg
    18241 gtttgtaatg ttttattacc tctattcaaa tttaattgga aagaggtttt ttattatgtc
    18301 tcgtaaaaat attaaaaatc aagcaagtca aaacttttat atgctacata aagcattatt
    18361 tgttaatgaa aaatataaaa aattaagtga tagtgccaaa gttacttatg caattctcaa
    18421 cgatagagtt agcttgtcga ttaaaaataa ttgggtcgat gataatgggg atatatattt
    18481 catttttaca aatgaaagtc tccagaatat attagacaaa agcaagaata caattactaa
    18541 aataaaaaaa gaacttcaag cagtagggtt acttgaacaa atacgtacag gatttaataa
    18601 acctaataaa ttatatttgc atgatataga aactaatatt agtgtagaaa aaagtattca
    18661 atcctcatct ataacctaca atgacaagga gtcccaaaat ttgggactcc agaatcccga
    18721 aatttgggac tccagaatcc cgaaatttgg gactccagaa tcccaaattt tagaccctaa
    18781 tgatactgat tataatgata ctgattatat taagactgag agtaatgata cggatgattt
    18841 gaatgataag aaattaacat atcctaataa tcatacaaat cattcaaatc acgacaattc
    18901 aaactttaat aatgaggctt taaaattcca attactcgaa gaactaccgc aaagtattca
    18961 aaactatcta agtaactttg aagtaactga aattgaaatt attaaaactg tattattaaa
    19021 agccaaaaca tctttcaaca atacgattga tagttattac ttgttagaag atatggaaat
    19081 cgaaatactt catgttctta aacgtttcaa agctatactt attcaaaaaa atgaaaccgt
    19141 tgaagccatg caaggatact taatgaaatc tcttaaatct gaattcgctg aaatgcatac
    19201 gcttaataaa cgacgtgatc atttaccaat cacttcttta tttaatcaat aatgatttaa
    19261 ttagagggca tctatttaaa gatgctctct ttttaattta caagtggttg ctaaggcaac
    19321 cacctagatc aaacgaaagt aagggagagg ataacaaaat aacatcaaca caatgtcaca
    19381 acatagaatg ctgctattaa agaagggagg aacacatatg agtaataaga aaaagattaa
    19441 agttacattg gcaatggtta atgttctttt aactgcagtc gaactttatc ttcaatggca
    19501 attgagtaaa tctgaaaatg aactgtagtt aagcaagcag gtgggactaa aacagaaacg
    19561 ttttagtcct atttttttct ataatatatt tatattcgtt taataatgac tcaaacatgt
    19621 cgcataagat gaaaaataaa atgatcattt agcactgttt taactttaaa aaatatagct
    19681 aatcactaat atatcatgtc agtgttcgtt taacttgcta gcatgatgct aatttcgtgg
    19741 catggagaaa atccgtagat ctgaagagac ctacggttct ttttatatag agcgtaaata
    19801 cattcaatac cttttaaagt actttttgcc gtattgatac tttgatatct tgtctttctt
    19861 actttaatat gacggtgatc ttgctcaatg aggttattca gatatttcga tgtacaatga
    19921 cagtcaggtt taagtttaaa agctttaatt actttagcca ttgctgcctt cgttgaaggt
    19981 gcctgatctg taactacctt ttgaggctta ccaaattgtt taatgagacg tttgataaac
    20041 gcatatgctg aatgattatc tcgttgctta cgcaaccaaa tatctaatgt atgtccctct
    20101 gtatcaatgg cacgatataa atagctccat tttcctttta ttttgatata cgtctcatca
    20161 atacgccatt tgtaataagc ttttttatgc tttttcttcc aaatttgata taaaattggg
    20221 gcatattctt gaactcaacg gtagatcgtt gaatgatgaa cgtttacacc acgttctctt
    20281 aatatttcag atatatcacg atagctcaat gcatatctta gatagtagcc aacggctaca
    20341 gtgataacat ccttgttaaa ttgtttatat ctgaaatagt tcatacagaa gactcctttt
    20401 tgttaaaatt atattataaa ttcaactttg caacagaacc caaaaagttg cctttagcat
    20461 ctcaaatcca ttgtcgctct aaggatcata aggtgcctaa aaaggtttta aaaagattta
    20521 taaaaagata ttaaaaagtt cggggcattt tgcccctcac aaattcaaaa gacaaaaagg
    20581 aaagcaacca cttttgtggt tgctttttta tttataaaaa tcattaagtt attacccttt
    20641 aatgcattat tccaattgct ttattgacat tgagccgcgg aacccttaac aaacccaaaa
    20701 cttgtcgaat ggtcggcata atagcgtgta ttactttttg tttttctttt ctgaataagg
    20761 taaataaaag atgacgaata gagcacatgc aatcgctata aaagaaccta taaaaaccaa
    20821 ataatctcct ccttttttag ctttatacag tgtctttacg tataaatata tatttaacct
    20881 aaaaaataga ttaaagtcgt tcatacgcct taaaaatggc atatatgagt aaactaaata
    20941 agatgtaagg cacaaaataa aacatcacga tcagatatcg ccatggtgaa tgacctgttt
    21001 ttaagacgtg gttaatccag tcataaaaat gttgtacgcc taataagtcg cctaatggac
    21061 ttaagactac aaaagtaatc attactaagg caacgatgaa aaacatcgct gtaatccctt
    21121 tagttaaacg attgaacgct tgatcatatt gttttattga cgtttcgttc gttgcgatgc
    21181 gctcatcaag cgttttttta tagtcatcat gtgctttttg taggtctgat tgatgatttt
    21241 gaaactcttt aactagattt tgtgtttgtg atttcacatc ttctaattct tgttggattg
    21301 ctgattgaaa atcttgtttt aaattatcgt tattaatacg ttccacatag tgttttgcaa
    21361 cgctaataaa tcttttttgg gttttttctg tggcttgatt aaagttcaag gccatagtct
    21421 ctatcgttgt caataattgt tcgtgacgct tgttccgctc gttctctctc ttctcgatct
    21481 cttcgagcct tttcatcagc aagtcttgct gactctgcag cttgtcggcg acgctcaagc
    21541 tcatttgtgt cttgggcgaa tttatcccaa tctgattgag tgacgcctgt atctcgttga
    21601 gttggttcat cacggtttga agttggtcgt tttggctgga tgtcgttgtc atcttctcgt
    21661 gcttgctgtt gtcgttgtat ttgtctttca aagccatctt ctatgccccc tttgttataa
    21721 tcttcaccta atcgacggcc acggactttt ttatcttcag ctagatgtcg ataggtaatg
    21781 ctcttaggtg taactcgtgc aatttcaatg ccatattgac ttaaatggtc tttaaattcg
    21841 tccatatttg tagctgttga ttggtcgatt gcttcacgta tgtcatcttt ccatgaatat
    21901 tgagcggttg attttgtatt agggtcagca atcgcttttt ctgtttgtgt ataacgtaag
    21961 cgtgctgtat ctttttctgg cacgcttaag ccatgttcac gacatacgtc gtcattaaag
    22021 tttcttaaat catgtaaggc ttgcttgttg ttattgaact ttttacctgt ttcaagatta
    22081 acggcattaa tcacaatatg attatgtatg tgatcagtat cacaatgcgt ataaacagcc
    22141 acttgatgat tggttgcgaa tttttcagct aatgctaagc ctagttgatt acattgttct
    22201 ggggttactt cgcctggttt gaatgactga ataatgacat ggccttcatt accccctgtt
    22261 ttaccataga gttcacgtgt ggctttaaaa ctacttttgg catagtctat gtcgcagttt
    22321 aagccacttt tttcttcagc ccgcttttca gcgtaattga tggcacgtga cgttgatttc
    22381 gtcgcactta atttagttgt tgccatatgt catccaacct ttcacgtaat tcactgatat
    22441 tacgttctaa tgcgctataa tttggtgcgt catattgatg ttgatgacaa tatttcgcaa
    22501 tttgattagc attcgcccct aatcgactta aatctttcgc aatcgattgt cgcgtttctt
    22561 tatcaaattt aggtgcgacc aatcgactgc cttgtgcctt tttcttaacg aaagcaggca
    22621 cactcatatt taaagtttca gccgactgct ttaactttaa atattcggat tcgctcacac
    22681 gaaaactaat ttgtttcggc tccttacggt tgggtttatg gttttgccca acagtttcgt
    22741 cgctagccac aaaatgattt tgttcgctca tacatcccac cacgctttct acgaaagata
    22801 ttacttaata taaatttaga ttatatgata atctaaattt catcaagtaa tatagtggtt
    22861 tggcaagcac tggctttgct agccactaac gtggtagcga cgccggcttg ttggggataa
    22921 tccccaagcc ccttatgttt ttgctaaagc aaaaaatgag tggcaaaatg ctagccactc
    22981 atagttaaaa aaattcaaaa tcaaaacctc tgcaaccaaa aatgtggttg ctcataaatg
    23041 aagttctgaa aagcaaagat tatttattaa ggtgttatgc gtattaaata ttcttttttt
    23101 ctatgtttta atcaaatttt tacagttatt aatacttata ttctatttga tattcagaaa
    23161 tatttttacg tcctttttct gttaactctg cgtatcgtgt tgtcccataa atcggaaaac
    23221 atttattata aaacaattct acattttcag ctaaatcttg tctcgctata ctctcgagtg
    23281 ctaataaaaa agtttttcac tcacatttaa cgttttaaaa tctacacttt ctccttcttt
    23341 aatcgataac agtacactat acactaaatc ttgttttaac aacgttcatc acctcttatt
    23401 tatctagatg acgatacaaa gttgctctac ttatatttgt ctgttctttt atctcatcta
    23461 aagtgtattt tttactcata tacatttcaa tagcatcact aagatttttg tcgtttcttt
    23521 tgggtcttcc taagtttttt ccttcttttg cataattttc taaacctaaa cgagttctaa
    23581 atttgacaat gtcactttgg aagtctgtga tatcatgtaa tatttctata aattcacctt
    23641 tgatgtcttt ataaatataa cgatttaaat taataacata taaagatata tcctcctgag
    23701 aaagataatc taatatatct actaaatgtt tcgtagaatc tgcaagaata cataaatccg
    23761 taacgtataa catatctcca gaaactaatt tttcatttaa aagaacatct aatatattcc
    23821 tcttcttatt attagcatga atttcttcaa caatttcatt tgtatgagtt tctattttct
    23881 ttttttgttc atttaaatcg tcgtttattg taacgggtct aatataaccg tatttcattt
    23941 aattgcacca ccctgtagct aaagtttaac acaaagtttc attaaggggt atacatatga
    24001 aacttttctg atatacttta caatataatt aaataaaaag gagagtaagt tatggttgaa
    24061 aaattaaaaa atgaatggct taaccagcca ggcaaaaata tacttgcagg tattgtagtt
    24121 gccttagctt taatccctga agctatcgca ttctccatca ttgctggtgt agatcctatg
    24181 gttggtttat atacttcgtt cattattgct gtcgtcactg ctattgtagg tggtagagcg
    24241 gcgatgatat caggtgcaac aggggctatt gccttattaa ttgtgccact tgtgaaagat
    24301 tacggtgtag agtatcttct agctgctact atactgatgg ggttaattca attaatttta
    24361 ggacttctta aagtaggtcg tctaatgaaa tttatacctc gttctgtcat gataggtttt
    24421 gtgaatgctc taggaatcat gatttttatg tctcaaatag aacatatctt tgggatatca
    24481 atttcaactt atatatatgt aattgtaacg ttactcatcg tatatataat tccaagattt
    24541 tttaaagcta tacctgcacc attaatagct attatcgtat tgactgccct ttatatgtat
    24601 acaggttctg acgttagaac tgtgggagat ttaggtaata tcaaacaagc tttccctcac
    24661 tttttaatcc ctaatgttcc ctttaattta gaaactcttc aaattatttt cccttactct
    24721 ttatctatgg ctattgtagg tttagttgag agcttactta cagctaaaat tgtagacgat
    24781 gctacagaca cttatagtag taaaaataga gagtcctgtg gtcaaggtat cgctaacatg
    24841 attaccggat tattcggtgg catgggaggt tgtgccatga ttggtcaatc cgtcatcaat
    24901 gttaaatcag gtgcaaacag tagattgtct acatttacag cgggtattgt acttatattt
    24961 atgattattg tacttggagg acttgtagtt caagttccaa tgccaatttt agcaggtatt
    25021 atggttatgg tttctgttgg tacatttgat tggaattcct ttaaatatat taagaaagca
    25081 ccaaaaacgg atgcagtcgt tatgcttatt acagtgatta ttgttttaat gacgcataat
    25141 ttagctttag gtgttgttgc aggcgttgtt ttcagtgctt tattctttgc tactaaaatt
    25201 tcaaaagtag aagttatttc tgaagagttc ggtaaaataa accgattatc ttttaaagga
    25261 caaatctttt ttgtttctat tgattctatg atggagcaaa taaactttga gattgaaaat
    25321 agtaccatag aattaaactt caataatgca cacttatggg atgattctgc agtagatgca
    25381 attgacacga tagtacgaaa attcgaagaa aaaaataata ttgttcgtgt agaaaaatta
    25441 aattcagata gtcgtaaagt tgtttcagag ttaagccaac taaatgaaaa tcatttaaac
    25501 taaaggagcg ttatttatgt ataaatcaat tttattagca gcagatggat cagagaatag
    25561 tttgcgttca gcaaaagagg ttttaaattt tatagatgat aatacgattg ttactattct
    25621 tacagttgta gatgttgagg aatcgaaaac agatgtttta catggtaaac aaagttccag
    25681 tttgacgaat gaaagagaag acaaactctc tcatatcact gagttatttg tagagcataa
    25741 tgtaaaatat gaaatgaaaa ttgcacatgg tgttcctgcg gacacagttg tttcagttgc
    25801 caatagtgga gaatttcaag ctattgtttt aggaacacgt ggattaaata gtttgcaaga
    25861 aatggtgtta ggtagcgtca gtcacaaagt agcaaaacgt tcagaaattc ccgttgttat
    25921 tgtaaaatag tctgtctaac tcaatccgtg aaaggattga gttttatttt agaggtgaat
    25981 attagatgaa aaccaaaaat ttctattatg gttgggttat tgtatttatt gctggtttga
    26041 cagttttctt ctctggtcct ggccaaactt attcaaacgc agcatttatt gatgaatata
    26101 ttcaaacatt tggatggagc cgtacagaag tttcctcaat atattcttta gctattctat
    26161 ttggaatcat atggggaatt gcgaacggtc tagaaagaat tggaaccaat attgtttggg
    26221 ctaactactt tggacgtaag catgtaggga gtataaatgg aattgaatca actatgctag
    26281 tgattggttc ctctttagga gcattaccat taggcctagg tcatgatatt ttccaatcct
    26341 attcattaac tatttatatg atgattattt tccccattat tggtttagtt tgtgcattat
    26401 tggctaaaaa gccagttaaa aattattaaa atataccata gaaagaatgg tatttaaggg
    26461 gagatgttat gaagttttgc aaaggaatat gtattctaat attattggtt ttttgtgatt
    26521 tgttgatttt taaacggtcg aaagtatggg gagtagagca tgaatcgagt aataatcaaa
    26581 ttcgaataat aaaagaagaa gactatacag atatggttat taaccaatac taatcatatt
    26641 ttagtataac aatatataga taaacctctt tataaaaatg gatatgttat acaaactaac
    26701 tacctataag aactaattat gttaactaac tcaaaaggct taaacgctgt taaatcaacg
    26761 tttaagcctt tttttaatga ttatcgttta tgcaggtttt atacatcatt gatctgatgc
    26821 gttttcatgc attttcatga gttaatattt tgtattttta tgtagtttat acgtgtatac
    26881 aaagtataca ttggctacat tgtatacttt gcatattttg aatacttttt tgaaggctca
    26941 tgtattcgtc aaatatcacg ttattatatt atacttgtaa ggcacttccg tgcacaaagg
    27001 aggtgtgcat atggtgttaa tgatactctc acttgttgga acatttatct tatcagttat
    27061 tgctggtgta gttgcgaact acatctcaga taagctcgat agataaataa aaaaacccca
    27121 ctttgctttg gacggcagtg gggttttcgc atatggcgaa taatacttca cttgttaact
    27181 tcagtatatc tttcatagta tcttttgtaa actttctaat ctttctaatc ttcccaatct
    27241 agttctaatt gttgtctaaa tgcttgttgt tgttctttta atagtgcttt ttcttcttcg
    27301 gtcatttctc tagttgtatt actttgattt cgttcatgta gccattttgg cgttttctca
    27361 cgactgatat attgtttagg ttgggctaca tttgccattt taggttgtcg cttactatga
    27421 atgcgttttt cagcatcaaa agtaaactct aaccattcta ttttacgtcc ttttttcgct
    27481 ttgattttat tgatatgtag attattaaaa ataaagccta actctttaat gataggggct
    27541 aaaacttttt gattgatatg cgtcatacgg tagctattag gaatatctaa tcgttctcta
    27601 aaatcctcaa tttgaatttt gaaataacct gtgtgtttat attgttttaa taacctaaac
    27661 atatttttag agtatgttga tttaagatgt gtcatttctt ttaattcaaa cttagtaaaa
    27721 tcagctgtta tttcatttaa aacatgcttt aatttaggat taatactaac aattaatttt
    27781 tgttcatcaa catcaacttc atatcctgta aacaatacaa attttttgaa agatagacca
    27841 cttcgttcag tataagttaa agttaacatc ttatcgtata catgttctaa gtctttaaca
    27901 aaacgtttta aagaacgatg ataataatta cttaagtgac gtaactcctc aaaagggata
    27961 gctaattctt ttatatcttg ttcttttaat ttattacaaa gactaaaaaa ggttctgttg
    28021 caaagtaaaa aatatagcta atcactaata tatcatgtca gtgttcgttt aacttgctag
    28081 catgatgcta atttcgtggc atggggaaaa tccgtagatc tgaagagacc tacggttctt
    28141 tttatataga gcgtaaatac attcaatacc ttttaaagta ttctttgccg tattgatact
    28201 ttgatatctt gtctttctta ctttaatatg acggtgatct tgctcaatga ggttattcag
    28261 atatttcgat gtacaatgac agtcaggttt aagtttaaaa gctttaatta ttttagccat
    28321 tgctgccttc gttgaaggtg cctgatctgt aactaccttt tgaggcttac caaattgttt
    28381 aatgagacgt ttgataaacg catatgctga atgattatct cgttgcttac gcaaccaaat
    28441 atctaatgta tgtccctctg tatcaatggc acgatataaa tagctccatt ttccttttat
    28501 tttgatatac gtctcatcaa tacgccattt gtaataagct tttttatgct ttttcttcca
    28561 aatttgatat aaaattgggg catattcttg aacccaacgg tagatcgttg aatgatgaac
    28621 gtttacacca cgttctctta atatttcaga tatatcacga tagctcaatg catatcttag
    28681 atagtagcca acggctacag tgataacatc cttgttaaat tgtttatatc tgaaatagtt
    28741 catacagaag actccttttt gttaaaatta tattataaat tcaactttgc aacagaaccc
    28801 tttgaacttc tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga ttgtgctgaa
    28861 atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg agtgtggttt
    28921 tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag caatgaaaca tggcattcag
    28981 tcacaaaagg ttgttgctga agttattaaa caaaagccaa cagttcgttg gttgtttctc
    29041 acattaacag ttaaaaatgt ttatgatggc gaagaattaa ataagagttt gtcagatatg
    29101 gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct tgttggtttt
    29161 atgcgtgcaa cggaagtgac aataaataat aaagataatt cttataatca gcacatgcat
    29221 gtattggtat gtgtggaacc aacttatttt aagaatacag aaaactacgt gaatcaaaaa
    29281 caatggattc aattttggaa aaaggcaatg aaattagact atgatccaaa tgtaaaagtt
    29341 caaatgattc gaccgaaaaa taaatataaa tcggatatac aatcggcaat tgacgaaact
    29401 gcaaaatatc ctgtaaagga tacggatttt atgaccgatg atgaagaaaa gaatttgaaa
    29461 cgtttgtctg atttggagga aggtttacac cgtaaaaggt taatctccta tggtggtttg
    29521 ttaaaagaaa tacataaaaa attaaacctt gatgacacag aagaaggcga tttgattcat
    29581 acagatgatg acgaaaaagc cgatgaagat ggattttcta ttattgcaat gtggaattgg
    29641 gaacggaaaa attattttat taaagagtag ttcaacaaac gggccagttt gttgaagatt
    29701 agatgctata attgttatta aaaggattga aggatgctta ggaagacgag ttattaatag
    29761 ctgaataaga acggtgctct ccaaatattc ttatttagaa aagcaaatct aaaattatct
    29821 gaaaagggaa tgagaatagt gaatggacca ataataatga ctagagaaga aagaatgaag
    29881 attgttcatg aaattaagga acgaatattg gataaatatg gggatgatgt taaggctatt
    29941 ggtgtttatg gctctcttgg tcgtcagact gatgggccct attcggatat tgagatgatg
    30001 tgtgtcatgt caacagagga agcagagttc agccatgaat ggacaaccgg tgagtggaag
    30061 gtggaagtga attttgatag cgaagagatt ctactagatt atgcatctca ggtggaatca
    30121 gattggccgc ttacacatgg tcaatttttc tctattttgc cgatttatga ttcaggtgga
    30181 tacttagaga aagtgtatca aactgctaaa tcggtagaag cccaaacgtt ccacgatgcg
    30241 atttgtgccc ttatcgtaga agagctgttt gaatatgcag gcaaatggcg taatattcgt
    30301 gtgcaaggac cgacaacatt tctaccatcc ttgactgtac aggtagcaat ggcaggtgcc
    30361 atgttgattg gtctgcatca tcgcatctgt tatacgacga gcgcttcggt cttaactgaa
    30421 gcagttaagc aatcagatct tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct
    30481 ggtcaacttt ccgactctga gaaacttctg gaatcgctag agaatttctg gaatgggatt
    30541 caggagtgga cagaacgaca cggatatata gtggatgtgt caaaacgcat accattttga
    30601 acgatgacct ctaataattg ttaatcatgt tggttacgta tttattaact tctcctagta
    30661 ttagtaatta tcatggctgt catggcgcat taacggaata aagggtgtgc ttaaatcggg
    30721 ccattttgcg taataagaaa aaggattaat tatgagcgaa ttgaattaat aataaggtaa
    30781 tagatttaca ttagaaaatg aaaggggatt ttatgcgtga gaatgttaca gtctatcccg
    30841 gcattgccag tcggggatat taaaaagagt ataggttttt attgcgataa actagg
//
