LOCUS       NZ_CP069952             6266 bp    DNA     circular CON 02-APR-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_1363 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP069952
VERSION     NZ_CP069952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357505
            Assembly: GCF_016903555.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1898.18x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016903555.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:37:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,337
            Genes (coding)                    :: 2,275
            CDSs (with protein)               :: 2,275
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 32 of 62
            Pseudo Genes (incomplete)         :: 38 of 62
            Pseudo Genes (internal stop)      :: 21 of 62
            Pseudo Genes (multiple problems)  :: 23 of 62
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069952.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1363"
                     /culture_collection="FDA:FDAARGOS_1363"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MD"
                     /collected_by="FDA-Stanford Microbiome Effort"
     gene            join(6032..6266,1..773)
                     /locus_tag="I6K93_RS11830"
                     /old_locus_tag="I6K93_11830"
     CDS             join(6032..6266,1..773)
                     /locus_tag="I6K93_RS11830"
                     /old_locus_tag="I6K93_11830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_145366607.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKATRE
                     LYGKTGGNEGHVIIQSFKPGEVTPEQCNQLGLELTEKFAPNHQVAVYTHNDTDHVHNHI
                     VINSIDLETGKKFNNNKQALRDLRDFNDDVCREHGLSVPEKDTARLRYTQTEKAIADPN
                     TKSTAQYSWKDDIREAIDQSTATNMDEFKDHLSQYGIKIARVTPKSITYRHLAEDKKVR
                     GRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDESTERDTGVTQADWE
                     QFAQDTNELERSRQAAESARLAHEKARRDREERAREKQASRTIIDNDRDHGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            739..1281
                     /locus_tag="I6K93_RS11835"
                     /old_locus_tag="I6K93_11835"
     CDS             739..1281
                     /locus_tag="I6K93_RS11835"
                     /old_locus_tag="I6K93_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_202596020.1"
                     /translation="MTTIETTASNFNQATEITQKRFISVAKHYIERINNDNLKQDFQTA
                     IQEELEDVKSQTQNLVKQFQNHQSDLQKAHDDYKKTLDERIETNEIAIKQYDQAFNRLT
                     KGITAMFFIVALVMITFVVLSPLGDLLGVQHFYDWINHVLKTGHSAWRYLIVTLYLAPY
                     ILFSLLIYAILKAYERL"
                     /name="mobilization protein"
     gene            2032..2895
                     /locus_tag="I6K93_RS11840"
                     /old_locus_tag="I6K93_11840"
     CDS             2032..2895
                     /locus_tag="I6K93_RS11840"
                     /old_locus_tag="I6K93_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_145366611.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYSPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     EKYLEIATSPDLKYILNSITNNFTKFELKEMTHLKSTYSKNMFRLLKQYKHTGYMKIKI
                     EDFRERLDIPKSYRMSNINQFVLTPIIKELSPIFNNLNINKIKAKKGRKIEWLEFTFDA
                     EKRIHSKRQPQMTDVGKSRQYISREKTPKWLEERTYEKTTQNEYDPQLEKEREAFLKQL
                     QVDWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(3030..3125)
                     /locus_tag="I6K93_RS11845"
                     /old_locus_tag="I6K93_11845"
     CDS             complement(3030..3125)
                     /locus_tag="I6K93_RS11845"
                     /old_locus_tag="I6K93_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001002795.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3549..5117)
                     /locus_tag="I6K93_RS11850"
                     /old_locus_tag="I6K93_11850"
     CDS             complement(3549..5117)
                     /locus_tag="I6K93_RS11850"
                     /old_locus_tag="I6K93_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449123.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vga family ABC-F type ribosomal protection
                     protein"
                     /protein_id="WP_145366609.1"
                     /translation="MKIMLEGLHIKHYVQDRLLLNINRLNIYQNDRIGLIGKNGSGKTT
                     LLHILYKKIVPEEGIVKQFSHCELIPQLKLIESTKSGGEVTRNYIRQTLDKNPELLLAD
                     EPTTNLDNNYIEKLEQDLKNWHGAFIIVSHDRAFLDNLCTTIWEIDEGRITEYKGNYSN
                     YVEQKELERHREELEYEKYEKEKKRLEKAINIKEQKAQRATKKPKNLSLSEGKITGAKP
                     YFAGKQKKLRKTVKSLETRLEKLESVEKRNELPPLKMDLVNLESVKNRTIIRGEDVSGT
                     IEGRVLWKAKSFSIRGGDKMAIIGSNGTGKTTFIKKIVHGNPGISLSPSVKIGYFSQKI
                     DTLELDKSILENVQSSSQQNETLIRTILARMHFFRDDVYKPISVLSGGERVKVALTKVF
                     LSEVNTLVLDEPTNFLDMEAIEAFESLLKEYNGSIIFVSHDRKFIEKVATRIMTIDNKE
                     IKIFDGTYEQFKQAEKPTRNIKEDKKLLLETKITEVLSRLSIEPSEELEQEFQNLINEK
                     RNLDK"
                     /name="Vga family ABC-F type ribosomal protection protein"
     gene            5667..6050
                     /gene="mobC"
                     /locus_tag="I6K93_RS11855"
                     /old_locus_tag="I6K93_11855"
     CDS             5667..6050
                     /gene="mobC"
                     /locus_tag="I6K93_RS11855"
                     /old_locus_tag="I6K93_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048668204.1"
                     /translation="MSEQNHFVASDATVGQNHKPNRKEPKQISFRVSESEYLKLKQSAE
                     TLNMSVPAFVKKKAQGSRLVAPKFDKETRQSIAKDLSRLGANANQIAKYCHQHQYDAPN
                     YSALERNISELRERLDDIWQQLN"
                     /name="mobC"
     ARG             complement(3553..5117)
                     /name="vga(A)"
                     /gene="vga(A)"
                     /gene_name="ABC-F type ribosomal protection protein Vga(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="99.81"
                     /coverage="100.0"
     ARG             complement(3550..5117)
                     /name="vgaA"
                     /gene="vgaA"
                     /gene_name="vga-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic; streptogramin
                     antibiotic; streptogramin A antibiotic; pleuromutilin
                     antibiotic"
                     /identity="99.23"
                     /coverage="100.0"
     Typing          complement(5461..5696)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.326"
                     /coverage="100.0"
                     /evalue="5.88e-114"
     Typing          2033..2895
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7..770
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="94.0"
                     /evalue="6.070000000000001e-129"
     Typing          6033..6265
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.615"
                     /coverage="82.0"
                     /evalue="3.94e-35"
ORIGIN
        1 taactgaaaa attcgcacct aatcatcaag tcgctgtcta tacacacaac gatacggacc
       61 atgtacacaa tcatattgtg attaattcaa ttgatcttga gacagggaaa aaatttaaca
      121 acaataagca agccttacgt gatttaagag actttaatga cgacgtatgt cgtgaacatg
      181 gcttaagcgt gccagaaaaa gatacagcac gcttacgtta tacacaaaca gaaaaagcga
      241 ttgctgaccc taatacaaaa tcaaccgctc aatattcatg gaaagatgac atacgtgaag
      301 caatcgacca atcaacagcc acaaatatgg acgaatttaa agaccattta agtcaatacg
      361 gcattaaaat tgcacgagtt acacctaaga gcattaccta tcgacatcta gctgaagata
      421 aaaaagtccg tggccgtcga ttaggtgaag attataacaa agggggcata gaagatggct
      481 ttgaaagaca aatacaacga caacagcaag aacgagaaga tgacaacgac atccagccaa
      541 aacgaccaac ttcaaaccgt gatgaatcaa ctgaacgaga tacaggcgtc actcaagcag
      601 attgggaaca attcgcacaa gacacaaatg agcttgagcg tagccgacaa gctgcagaat
      661 cagcaagact tgctcatgaa aaggctcgaa gagatcgaga agagagagca cgagagaaac
      721 aagcgtcacg aacaattatt gacaacgatc gagaccacgg cctcgaactt taatcaagcg
      781 actgaaatca cacaaaagcg tttcatcagt gtagcgaaac actatatcga acgcattaat
      841 aacgataatt taaaacaaga ttttcaaacg gcgatacaag aagaattgga agatgtgaaa
      901 tcacagacac aaaatttagt taaacagttt caaaatcatc aatcagactt acaaaaagca
      961 catgatgatt ataaaaaaac gcttgatgag cgtatcgaaa caaacgaaat agcaataaaa
     1021 caatatgatc aagcattcaa tcgtttaact aaagggatta cagccatgtt tttcatcgtt
     1081 gccttagtaa tgattacttt tgtagtctta agtccattag gcgacctatt aggcgtacaa
     1141 catttttatg attggattaa ccacgtctta aaaacaggtc attcagcatg gcgttatcta
     1201 attgtgacgc tttatcttgc cccttacata ttatttagtt tgcttatata tgccatttta
     1261 aaggcgtatg agcgacttta accttagtca gtattaaaaa cctataactt atcatgaaaa
     1321 acgctgcata cggtttaaat tagtttagaa atggaattaa acatttttta taaggagatc
     1381 cgaaatgctt atatttttgt ttattttaat gagtggactt ttaagttatt acttgattaa
     1441 tcgttcaaac aaaaagaaat gacgctatta tgccgagaaa atttattgtg cattgagaag
     1501 aacccttaat aaaccctaaa actgtttcga cgaatgtcgg tatagcgtga gctataaaac
     1561 cgacgagtcg acagttttag ggtttattaa gggttcagag gctcaatgtc aataaagcaa
     1621 ttggaataat gcaataagta aatgattttt attaatcaaa accaatcata ttttatgatt
     1681 ggtttccttt ttgttttttt gaattttgag gggcaaattg ccccgaactt tttaatatct
     1741 ttttataaat ctttttaaaa cctttttagg aggtgcttta gacctagagt gccaaggatt
     1801 atagaatgtt aaaggcatag ttttgccaca ttaaaggcat agttttgcca cattaaaggc
     1861 atagttttgc cacattaaag gcatagtttt gccacattaa aggcatagtt tttttgttgt
     1921 tatgcctatt attctatgtt actgtataaa caagctttaa tacataatat tatagaattt
     1981 aaaggcatag tttttttgtt gttatgcctt tattaaaagg aggattatta tatgagcgga
     2041 gaaactgtta tttacaaaaa tgaaatgaat ttagttccat taagacgctt tactgctact
     2101 gaaattaact tattttttgc tatgtgtaat aagctcaaag aacaagatac taatacactt
     2161 catttgtctt ttgacgaact caaagaatta agtaattaca gtccagaaac acgtaacatc
     2221 aacaggtttg tagatgatct ccaaagagtt tacgataaga tgttagatat cagatatact
     2281 attagaactg aaacaaaaat taaaaaattt gtcttattct ataagtatga aatagatata
     2341 tcggaaaaat atttagaaat tgctacttct cctgatttaa aatatatctt aaattcaatt
     2401 acgaataact ttactaaatt tgaattaaaa gagatgacac accttaaatc cacctattca
     2461 aaaaatatgt ttagattact taaacaatat aaacatactg gctatatgaa gattaaaatt
     2521 gaagacttta gagaacgatt agatattcca aaatcttata gaatgtctaa tattaaccaa
     2581 tttgttttaa caccgattat caaggagctc tctccaatat ttaataacct caatatcaat
     2641 aaaatcaaag ctaaaaaagg tcgtaaaatt gagtggttag agtttacgtt tgatgctgag
     2701 aaacgcattc acagtaaacg acaacctcaa atgacggatg taggtaagtc acgacaatat
     2761 atcagtcgcg aaaaaacgcc gaaatggtta gaagaacgca catatgaaaa aacaacacaa
     2821 aatgagtatg atccacaatt agagaaagaa cgagaggcat ttttaaaaca acttcaagtt
     2881 gattgggaag aatagaaaag ttagaaattg agacttatgt aatatcatga tattatataa
     2941 gtaacaagaa tttcgtttat aaacgcacca aaccccgaac tattcacagt agttcggggt
     3001 ttttgggtac attgactgaa tcgtctatgt tattttttat cacgtttatg gagccaataa
     3061 ccaaataacg taactagaca accactgatt gccgttgtca tgatgtgaac aagaatttca
     3121 ttcataaagc gcacctcccc tctacgtcaa attgacgtct gaggaataga cgacttccca
     3181 attataacaa aatgtctcta aacttgtatg cctgcataca atatacatgc atacatgata
     3241 cacgtatact gtatgcaaca ctcagacgct tgagaactga ataaagatac aaaattttat
     3301 ttaatgaaaa tgcatgaaaa cgcgtagtta agggaatgta taaaatatga ataatccatg
     3361 aattcacata aaagggacaa acgccgtcat ttcagcgttt gtcccttttg gattggttaa
     3421 cataataaag tgttatgggt agttgaattg tatatgttat gcaatacgct aacttaataa
     3481 atattaccca tcaatgattc aataaaaaga caacaagaat aatctttatt gcttaaattt
     3541 aaagtaattt atttatctaa atttcttttt tcatttatta agttttgaaa ctcttgttct
     3601 aattcttccg aaggttcaat actcaatcga ctgagtactt ctgtaatttt tgtctcaagt
     3661 aaaagttttt tatcttcttt aatattcctt gttggctttt cagcttgttt aaattgttca
     3721 tatgtgccat caaatatttt tatttcttta ttatcaattg tcattattcg agtggctact
     3781 ttttcgataa atttacgatc gtgagataca aagattatac tgccattata ttcctttaac
     3841 aaagattcaa acgcctctat agcttccata tcaagaaagt ttgttggttc atctaatacc
     3901 aacgtattaa cttcacttaa gaatacttta gttagtgcta ctttaactcg ctctccacca
     3961 cttaagacac ttattggttt ataaacatca tctctaaaaa aatgcattct agctagaata
     4021 gttcgaataa gagtttcatt ttgttgtgaa gaagattgaa cgttttctaa aatactctta
     4081 tctaattcta atgtatctat tttttggcta aaataaccga ttttgacaga tggcgataat
     4141 gaaataccag gattcccatg cacaattttt ttaataaacg ttgtctttcc tgtaccatta
     4201 gatccgataa ttgccatctt gtctcctccg cgaatactaa aactttttgc tttccacaat
     4261 acccgtcctt caattgtacc cgagacatct tcaccacgta ttatagttct attttttaca
     4321 ctttctaagt tcactaaatc cattttaagt ggaggaagtt cgtttctctt ttcgacgctt
     4381 tcaagttttt ctagtctggt ttctagagat tttacagttt ttcgtaactt cttttgctta
     4441 cctgcaaagt atggctttgc tcctgttatt ttgccttcag ataaacttaa gtttttcggt
     4501 tttttagttg ctcgttgagc tttctgttct tttatattta tagctttttc caatcgtttc
     4561 ttttcttttt catatttttc atattctaat tcttctcgat gtctttctaa ttctttttgt
     4621 tcaacatagt tactataatt ccccttatat tcagttattc tcccctcgtc aatttcccat
     4681 atagtagtac acaagttatc taaaaaagcg cgatcatgtg aaactataat aaatgctcca
     4741 tgccaatttt ttaaatcctg ttctaatttt tctatatagt tattatctaa gttagttgtt
     4801 ggttcatctg ctaatagcag ttctggattt ttatcaagcg tttgccgaat atagtttcgt
     4861 gttacttcac caccactttt agttgattct atgagcttca attgaggaat aagttcacaa
     4921 tgtgaaaatt gttttacaat accttcttca ggcacaattt ttttatataa tatgtgaagt
     4981 aacgttgttt ttccacttcc atttttacca attaaaccaa tacgatcatt ctgataaata
     5041 tttaggcgat ttatgttcaa caataaacga tcttgaacat aatgttttat atgaagtccc
     5101 tctaacatta ttttcatatt ttctcatcct cttataagta attgaagaca ataaaagcct
     5161 cccaatctat tcagaatagg aggcataaat ctatatagat gaactctaac aaaatacttt
     5221 tagagccaac tacagaattt tcatcctatc ctgaaaagta caattgaaaa caacacaaat
     5281 caaacgctgt ttgtaaataa aacactttca attgtttact tttgaaaata ggattagtta
     5341 atcatgtagt tggttcaccc tttcctttat cgtattaaat tcagcttaaa caaatagcaa
     5401 ataaatgtca atgttcaact caaattattt ttttaattta tttatattac ttaatgaaca
     5461 gtggctagca attagccact tattttttcg cagaaaaaat cctaaggggc ttggggatat
     5521 tccccaacaa gcaggcgtcg ctaccacgat agtggctagc aaagccaatg cttgccaaac
     5581 cactatatta cttgatgaaa tttagattat catataatct aaatttatat taagtaatat
     5641 ctttcgtaga aagcgtggtg ggatgtatga gcgaacaaaa ccattttgtg gctagcgacg
     5701 caactgttgg gcaaaaccat aaacccaacc gtaaggagcc aaaacaaatt agttttcgtg
     5761 tgagcgaatc cgaatattta aagttaaagc agtcagctga aactttaaat atgagtgtgc
     5821 ctgctttcgt taagaaaaag gcacagggca gtcgattggt cgcacctaaa tttgataaag
     5881 aaacgcgaca atcgattgcc aaagatttaa gtcgattagg ggcgaatgct aatcaaattg
     5941 cgaaatattg tcatcaacat caatatgacg caccaaatta tagcgcatta gaacgtaata
     6001 tcagtgaatt acgtgaaagg ttggatgaca tatggcaaca actaaattaa gtgcgacgaa
     6061 atcaacgtca cgtgccatta attacgctga aaaacgggct gaagaaaaaa gtggcttaaa
     6121 ctgcgacata gactatgcta aaagtagttt taaagccaca cgtgaactct atggtaaaac
     6181 agggggcaat gaaggccatg tcattattca gtcattcaaa ccaggcgaag taaccccaga
     6241 acaatgtaat caactaggct tagaat
//
