LOCUS       NZ_CP069942             3179 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1332 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP069942
VERSION     NZ_CP069942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357474
            Assembly: GCF_016903715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 817x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 04:02:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,654
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 40 of 100
            Pseudo Genes (incomplete)         :: 63 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 23 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069942.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1332"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1332"
                     /db_xref="taxon:571"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(2924..3179,1..143)
                     /locus_tag="I6K62_RS28165"
     CDS             join(2924..3179,1..143)
                     /locus_tag="I6K62_RS28165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227630442.1"
                     /translation="MMIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELI
                     DASPYAKKYTSGVLSFAEQDLPPGQREKLMASFERVLMPGQGAAGAELPDPEHGASDRQ
                     AATAVLRPRRPSAHRCLADHSERQAGAA"
                     /name="hypothetical protein"
     gene            392..877
                     /locus_tag="I6K62_RS00010"
                     /old_locus_tag="I6K62_00010"
     CDS             392..877
                     /locus_tag="I6K62_RS00010"
                     /old_locus_tag="I6K62_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            881..1096
                     /locus_tag="I6K62_RS00015"
                     /old_locus_tag="I6K62_00015"
     CDS             881..1096
                     /locus_tag="I6K62_RS00015"
                     /old_locus_tag="I6K62_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1264..1596
                     /locus_tag="I6K62_RS00020"
                     /old_locus_tag="I6K62_00020"
     CDS             1264..1596
                     /locus_tag="I6K62_RS00020"
                     /old_locus_tag="I6K62_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1805..1975)
                     /locus_tag="I6K62_RS00025"
                     /old_locus_tag="I6K62_00025"
     CDS             complement(1805..1975)
                     /locus_tag="I6K62_RS00025"
                     /old_locus_tag="I6K62_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2614..2937
                     /locus_tag="I6K62_RS00030"
                     /old_locus_tag="I6K62_00030"
     CDS             2614..2937
                     /locus_tag="I6K62_RS00030"
                     /old_locus_tag="I6K62_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1265..1596
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2327..2518
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1864..2344)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2..1194
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.0"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2615..2934
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="8.1e-64"
ORIGIN
        1 aaggggcggc tggagctgaa cttcctgatc ccgaacacgg agcttctgac cggcaagcgg
       61 ctacagccgt attacgaccg cgccgaccgt ccgcgcatcg atgcctggca gaccatagtg
      121 aacggcaggc tggggctgca tgacccgaac gcgccggaga accggcgggt gctggtctcg
      181 ccttccgcgc tgccggaagc gaagcaggaa gccgcccagg cgattacgag cggcctgctt
      241 gcccttgcct catccgggga gctgaaaacg cgtcaggacg tcactgaggc gctggaaagc
      301 gcaggttttg aggtggtgcg caccacaaaa agcagcatca gcattgccga cccggacggg
      361 gggcgaaaca tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga
      421 cttagagcag aaatcgaaag cgcagcagca gactaccggc gagatgctga aagccgcatt
      481 cagcgagcac gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc
      541 cgccatcctc gaccacgacc ggaagctgtc ctcagccatg agccagcgca cgaaagggat
      601 gctgcgcatg gtcagccaga cgtggctgac catcgttctg gtctccgcgc tgctgatagc
      661 gtcgagcgcg ggcattctgt ggtggcaggg gcagcagata ctcgagaact atacgaccat
      721 ccgggagcag aagagcacgc aggccatgct gtcagagagg aacagcggcg tacagctctc
      781 gacctgcggc gagcagagac gccgctgcgt gagggtgaac ccggaagcgg gacagttcgg
      841 agaggactcg agctggatga tactggcggg gaaatagcac atgacggagc tggaaaaaca
      901 gttgctgagc gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga
      961 gaacgccttc gcggaatggc ggacgatgtc tggtcttatt caacgggaga acgcggcgct
     1021 gaacgagcgc gtcacggtct tgagcaggca ggtgcagagt ttgagcgagc agctgcgccg
     1081 gttgtcgaaa ggctgaacgc gatagaaact caccggcagc aggagcgggc ggtgcagcac
     1141 cagaaagcgc tggagctgga acgatcgcag tggcagcatc atggtccttc gctatgataa
     1201 gccgggttct attaattagt tgttatgtaa agatatttaa atttttcaac atgaggtatt
     1261 tttatgaata catatatttt cctgggaggg gctattgtgg ctgaagtcat aggcaccacc
     1321 tttatgaaat attcagaagg atttacacgc ctgtggccgt ctgtaggaac tgcaataagt
     1381 tattgcgctg ccttttatct gctttcccaa acattggaaa atatacctac cggtattgct
     1441 tacgcaattt ggtctggtgc gggaattgta ctaataagcc tagctggttg gttggttagc
     1501 ggtcaaaaac ttgattggcc cgcaattgca ggaatgttcc ttatttgcgt tggagttttg
     1561 gttattaata ttttttcaaa atcttctgcg cattaacttt ttaccgaagc atattggcca
     1621 aagcaaaagg atttccttga gatcctttct ttctgcgcgt aatctttttc cctgtaaaca
     1681 aaaaaaccac cgctaccaac ggtggttcgt ttgccggaat tcagagctac caactctttg
     1741 ttccgatggt aactggcttt cacggagcgc agatacctaa actgtccttt cagtgtagcc
     1801 gcactcaggc tttcacttca agaactctgt aggcattctc tacaatctct gctccgtgaa
     1861 cccagttacc aatggctacc gccactggcg atttgtcgtg tctttccggg ttggactcaa
     1921 gttgatagtt accggaacgg gcgtggtagt cggactgaac ggggggttcg tgcatacagt
     1981 ccatcctgga gcgaactgcc tacccggaac cgagtttcag gcgtggaatg agaaaccgcg
     2041 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg
     2101 agccaccagg gggaaacgcc tggtatcttt aagccgcatc gggtttcgcc accgctgatt
     2161 tgagcgtcat tttcgtgata ctcgtcaggg gggcggagcc tatggaaaaa cggcaactcc
     2221 gcatcctttt cttggccttt cctgcttcga gcggcatttg cttatcccgt aatctgtgag
     2281 ctggtaacgc ccgccgcagt ctcacggcag gagcgtagcg accgagtgag cgaggaggcg
     2341 tcatttcatc accacctgca ttttgcactt acgcccggca gccgcatttt tcccctgtca
     2401 catgaagcac tttcataaat tgccactccg tcataacatg tttagccagt atacactccg
     2461 ctagcgctac gtgactggtt cagggctgcg ccccgaaacc cgctaaaacc actgccgcgc
     2521 ctgcggcttg tccaacaccg tgccgaccgg gaaaagattt cccgtccgtc ccggcgttat
     2581 caggaggccg gattggcaga caaacgcagc aaaatgctca ccatgtgggt gactgaggac
     2641 gagcaccggc gtctgctgga gcgctgcgag ggtaagcaac tcgcggcctg gatgcggcag
     2701 acgtgcctgg acgagaagcc cgcacgcgcc ggcaaacttc cgtcgatctc gccggcgctg
     2761 cttcgtcagc ttgccggcat ggggaacaac cttaaccaga ttgcccgcca ggttaacgcc
     2821 ggtggtggaa gcggacacga ccgcgtgcag attgtcgccg cgctgatggc catcgatgcc
     2881 ggactcgagc ggttgcggca tgccgtactg gaaaagggtg ctgatgatga tcgttaagtt
     2941 tcatcctcgg gggcgcggcg gcggcggcgg gccggtcgat tatctgctgg gtaaagatcg
     3001 ccagcgcgac ggcgccagcg ttctgcaggg gaagccggat gaagtccggg agcttatcga
     3061 cgcctcgccc tacgccaaaa agtacacttc cggcgttctg tcttttgccg aacaggattt
     3121 accgcccggc cagcgcgaaa agctgatggc gagtttcgag cgggttctga tgcccggac
//
