LOCUS       NZ_CP069932             1557 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed23.
ACCESSION   NZ_CP069932
VERSION     NZ_CP069932.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069932.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed23"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..296)
                     /locus_tag="I6K64_RS29265"
                     /old_locus_tag="I6K64_29260"
     CDS             complement(<1..296)
                     /locus_tag="I6K64_RS29265"
                     /old_locus_tag="I6K64_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="WP_032192428.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQL
                     FSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGC"
                     /name="Rrf2 family transcriptional regulator"
     gene            complement(657..1397)
                     /gene="trmJ"
                     /locus_tag="I6K64_RS29270"
                     /old_locus_tag="I6K64_29265"
     CDS             complement(657..1397)
                     /gene="trmJ"
                     /locus_tag="I6K64_RS29270"
                     /old_locus_tag="I6K64_29265"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA];
                     GO:0008173 - RNA methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="WP_000940019.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQ
                     AIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEA
                     ANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLAT
                     QENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPE
                     SQELNILRGILASIEQQNKGNKAE"
                     /name="trmJ"
ORIGIN
        1 tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatt
       61 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
      121 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
      181 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
      241 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
      301 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
      361 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
      421 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtcggat
      481 gcggcgtgaa cgccatatcc gccctggggt tctgtacact gtaggcctga taagacgcat
      541 tacgcgtcgc atcaggcaac ggctgtcgga tgcggcgtga acgccttatc cgacctacgg
      601 ttctgttcac tgtaggcctg ataagacgca ttacgcgtcg catcaggcaa cggctgttat
      661 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
      721 aactcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
      781 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
      841 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgccg
      901 ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg aaccgccatc
      961 gccaggttca gcgagctgta ttccgggtta gccgcaatcg cgacatgata atggcatttc
     1021 tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa acaccagcgc caccggggta
     1081 tttgccgctt cagcgacgct tttcaggccg cattcgcgcg ggtcgagcat cggccatggc
     1141 agcgtgcggg aacgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
     1201 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     1261 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     1321 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     1381 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     1441 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     1501 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaag
//
