LOCUS       NZ_CP069931             1581 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed22.
ACCESSION   NZ_CP069931
VERSION     NZ_CP069931.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069931.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed22"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(105..533)
                     /locus_tag="I6K64_RS29250"
                     /old_locus_tag="I6K64_29245"
     CDS             complement(105..533)
                     /locus_tag="I6K64_RS29250"
                     /old_locus_tag="I6K64_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            691..861
                     /locus_tag="I6K64_RS29255"
                     /old_locus_tag="I6K64_29250"
     CDS             691..861
                     /locus_tag="I6K64_RS29255"
                     /old_locus_tag="I6K64_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            825..1253
                     /locus_tag="I6K64_RS29260"
                     /old_locus_tag="I6K64_29255"
     CDS             825..1253
                     /locus_tag="I6K64_RS29260"
                     /old_locus_tag="I6K64_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(1469..>1581)
                     /locus_tag="I6K64_RS29710"
     CDS             complement(1469..>1581)
                     /locus_tag="I6K64_RS29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_227630567.1"
                     /translation="PDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
                     /name="replication initiation protein"
ORIGIN
        1 cctgacagtg gtttagccag aagcagggct accaagacca atgcaataag taatatatcg
       61 ttttgctatc gtgcatcgct cgcgctcagt tccattgtgc ttttttaagc tgtcgttttt
      121 cttacggtat ataccggttt tttatggcgt ggtttcttaa cttgttcagc tactgatgga
      181 cccattgtat ctaggtagtc aactagcttt gttagatcat aaatattgcg accaccatat
      241 cggcgatcac tcttcgatgt tggtttcttg tccaagagat tagctttttc aagatcattg
      301 atagctctct gaacagtccg tacagaaacc cccatacgta tggctagact ttccattgac
      361 ggatgcggcc actcttgcaa actccaccag tgaacgatca ggttaagtag tgtgttaaag
      421 gccactgaag ttagcttttt ctcgttttgt ataaaaaaca atacggtagg cactgctgtc
      481 cagccaagag acaaaccgcc agctttccat ttattcttaa cggagtaagt cattgatttt
      541 cctaagcccc aaaatattta aagtatatat tatatggtat attcatatga atagggtgac
      601 actagcgcca ttattgtgca accaaaaaag actactctga aaacgaggaa aagatttttt
      661 cctgcctgaa ttagatacgg agttagcgat atgaaaaccg aacaacgtca tgatcttgtt
      721 aaagatattg aggtttttgg cgtatccttg tctctgttga tttccagagc gaatgagaag
      781 tctgttacaa tgccatctgg tctaagtcgg gagcagagaa gagcatgggc agcggagcag
      841 gcgcgcaaaa tccacaattg aatattgtct cattctctga gaccttcaac ctttattaca
      901 catccagata ttctgcaaaa acactcgata aaatcgatga tttcattgag cattttgaaa
      961 aatacaatct ctttggcgat cctttaaaag gatatccagc ttggactggc aaagtatcgc
     1021 catcgtggaa agtgcctgat cattacgaaa acaaagaagc tattgagaag tatgctagag
     1081 ctaacaaatt atggcatgct catttaggcg atccggtttt taaagatacg tttcatggga
     1141 aatacaaggt ttctgactgg gttattcatt tccagcggct gacaccgaac catataaagc
     1201 ttttagagct tggataccat gacccaatga agctacctca aaactttgaa tgatcgagcg
     1261 gcaggctaaa tgaaatcttg agattcattc agtctcgtcg taatctcact attgtaaaaa
     1321 cgaaaaaacc accctggcag gtggtttttc gaaggttagt taatcctggc agattctcta
     1381 accgtggtaa cagtcttgtg cgagacatgt caccaaatac tgtcctttca gtgtagcctc
     1441 agttaggccg ccacttcaag aactctcgtt acatctctcg cacatcctgc ttaccagtgg
     1501 ccgttgccag tggcgttaag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1561 ataaggcgca gcggtcgggc t
//
