LOCUS       NZ_CP069930             2724 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed21.
ACCESSION   NZ_CP069930
VERSION     NZ_CP069930.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069930.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed21"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..241)
                     /locus_tag="I6K64_RS29225"
                     /old_locus_tag="I6K64_29220"
     CDS             complement(<1..241)
                     /locus_tag="I6K64_RS29225"
                     /old_locus_tag="I6K64_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="WP_192479740.1"
                     /translation="MELKKLMGHISIIPDYRQAWKMEHKLSDILLLTICAVISGAEGWE
                     DIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSC"
                     /name="transposase family protein"
     gene            complement(327..1471)
                     /locus_tag="I6K64_RS29230"
                     /old_locus_tag="I6K64_29225"
                     /pseudo=""
     CDS             complement(327..1471)
                     /locus_tag="I6K64_RS29230"
                     /old_locus_tag="I6K64_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /name="ISAs1 family transposase"
     gene            1553..1738
                     /locus_tag="I6K64_RS29235"
                     /old_locus_tag="I6K64_29230"
     CDS             1553..1738
                     /locus_tag="I6K64_RS29235"
                     /old_locus_tag="I6K64_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncO"
                     /protein_id="WP_120795385.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKLH
                     FSNCYHNNQHHNSLHN"
                     /name="protein YncO"
     gene            complement(1653..2135)
                     /gene="ydcD"
                     /locus_tag="I6K64_RS29820"
                     /old_locus_tag="I6K64_29235"
     CDS             complement(1653..2135)
                     /gene="ydcD"
                     /locus_tag="I6K64_RS29820"
                     /old_locus_tag="I6K64_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="WP_064674391.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSVE
                     IYLSEKNIIYKIINDQKISRGNGHFISIMVNNYRAHCGVVDINLNFFNDILYSVRLKNI
                     SKLENMEFCATKQRVYFSDKNKKASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS"
                     /name="ydcD"
     gene            complement(2119..>2724)
                     /locus_tag="I6K64_RS29245"
                     /old_locus_tag="I6K64_29240"
     CDS             complement(2119..>2724)
                     /locus_tag="I6K64_RS29245"
                     /old_locus_tag="I6K64_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="WP_204724572.1"
                     /translation="HDEESGLYYNRHRHYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQ
                     VIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADA
                     MRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAK
                     CSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
                     /name="RHS repeat-associated core domain-containing
                     protein"
ORIGIN
        1 tacaggatac aactctggca atggtatcgt gaacaggaat accattttca aaatcaccat
       61 attgttttaa aaaatcgaga tgtgtttccc caaaatcctc tatatcttcc cagccttctg
      121 caccagaaat aacggcacaa atagtcaaca gtagaatatc tgacaactta tgttccattt
      181 tccaggcttg tctgtaatcg gggataatag aaatatgtcc catcaatttt ttaagttcca
      241 ttttgttctc cttaattatg taaggagtat ttgatcatgt ataagcaata aaaaacagct
      301 ttaggtaata aggaatatct caatttttaa acataaaatg cgaattgttt agtacaaaaa
      361 gtgaacttct gatgatcttt ccctgtgttt aacgcagcct tctttctttt cctcttcacc
      421 cttaatgtct ttgcaatctc ttaataaatt cagtgccatc ttctttattc cagatattat
      481 ttaggctgcg tttcctcttc ttatccggct ggcatcttca ttcattttta catctaacac
      541 ccaatgaaga ctgtgctcga tcagccagtg cgctctgata gcatgtgcaa attctttagc
      601 atccatatcc tttgatgaaa tataatatcg gatgcttaca ccttctgctg atttatcttc
      661 tttcttctgc ctgaatgaca atgctacaca aagctttttt aatcccttcc attcgaattc
      721 aaaatcacaa aagttcaggc gttacgttac tgacaatatg caaacgtgtt tcttttcttc
      781 catgacttat ctcctgcgta ctaaacgaat cgcctttata attagaaaac acatttacag
      841 gaaatttttc ctcgaatgca tgatgtaatt tcccctgatt gccttttact gccagaagat
      901 aatctgcttt tttatctttg atcttcgaag cgatatcttt ctgacagccc atagcatcaa
      961 tggttatcaa atttttcttt aaatacagta ggttaagcaa ctcttgaatg gctgtaatct
     1021 cattactttt ggcttccgtt ttcacctgcc ccagtacaac accattttcg ttcgagaatg
     1081 cactcaccat atggattgct ccttttcttt ttcccttatc aaaggagcct cttatggtct
     1141 ttccatctat tgctataatt tcaccatcag tgatttcatg gcactcctgc atccattcaa
     1201 taaacatctt ttcaaaggcc aaactgtcaa tgttactcac aacgcgtgca atggtgtcat
     1261 cgaccggaat gccattatca aaatcaccat atttctttag ccattcaagt ctttcatgtc
     1321 caaaatcttc aatttcctgc cactcatcgg cacctgcaat tactgcacat acggtgagaa
     1381 acaaaatagc agataattta tgtttaacct ttccttgttg tcgtatatca ggggtcactg
     1441 aaatataatc aagcaaactt tgaatactca cctggcgtcc ttttttatct ttttagttat
     1501 tccaaaggtt aaattttgat cacctgtagg cgttaaagca aagatgaaaa tcgtgattta
     1561 catcacaatt ttcatgatct tgccctgccc cgtcccttgc tctcatgtct ttctgtatgt
     1621 attttatatt tttctctttc tggtgttgtt cattatgacc atttaccaat ccagtcaaaa
     1681 acttcatttt tcaaattgtt atcataataa tcaacatcat aatagtctcc ataattaatt
     1741 attttataac tagccttctt gtttttatca ctaaaataca cccgttgttt tgtagcgcag
     1801 aactccatat tctccagttt actaatgttt tttaatcgca cgctataaag aatatcattg
     1861 aaaaaattaa gatttatatc aaccactcca cagtgcgccc tgtaattatt aaccattata
     1921 cttataaaat gaccattccc tctactaatt ttttggtcat taattatttt ataaataata
     1981 tttttttcag aaagatatat ctcaacgctc tttggtgttt tatgtccata aaatacgatg
     2041 tcagaatttg aattgttatc aaacccaagg taaacaacaa ataaacaaag ggtaattaaa
     2101 aaaatgacaa gtttgcctct aataatttga agcatctgaa ctcccttgct ttgcttttat
     2161 tggattatct gcttttatac catctaaccc gaagagttgc ccagtgttat atttttcaat
     2221 acatgcatcg ctacatttgg cagaaaaatc gccacaagca ataccgacag tgttattttt
     2281 aagatccatg attctttctt tagctggctg accttgtcta tcccctgccg cctcatgatt
     2341 tttacctatc acgtcagcta ttgttgatcc aaatgtctta gtcattcgac acatcaagta
     2401 acaatgtctc attgcatcgg cggccccatt atgagctccg ccaacaatag catccatatc
     2461 ataaccgaac tggtaagcac tgttagtaat gctgtttgca gcaatcaatc caggtacacc
     2521 agatacggca taaattagtc cacctgatgt catattctca atcgcatcta accccattgg
     2581 gtctatcact tgtatgggat tcaacggata ctgatacata ttccatccac ctctcaaccc
     2641 aatcgggtcc ggggtgatat accgcccctg caacggatcg tagtgccggt gacggttata
     2701 gtacagcccc gactcctcat catg
//
