LOCUS       NZ_CP069926             5191 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed17.
ACCESSION   NZ_CP069926
VERSION     NZ_CP069926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069926.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed17"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..2771)
                     /gene="rhsB"
                     /locus_tag="I6K64_RS28885"
                     /old_locus_tag="I6K64_28880"
     CDS             complement(<1..2771)
                     /gene="rhsB"
                     /locus_tag="I6K64_RS28885"
                     /old_locus_tag="I6K64_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="WP_204724565.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTS
                     GHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRL
                     QLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALP
                     EELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTF
                     HREAAGEFSGEITGVTDGAWRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYT
                     EYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDD
                     KYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVL
                     HTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITT
                     PDGRASAFYYNHHSQLTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDL
                     PCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSM
                     EYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLV
                     THWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVAVYYGYDEKGRLTGE
                     RQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPL
                     VEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSP
                     RQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSM"
                     /name="rhsB"
     gene            complement(3014..3220)
                     /locus_tag="I6K64_RS28890"
                     /old_locus_tag="I6K64_28885"
     CDS             complement(3014..3220)
                     /locus_tag="I6K64_RS28890"
                     /old_locus_tag="I6K64_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="WP_000424924.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLHR
                     VWSKTSDKPVWMDQAEKATGDFY"
                     /name="YbfA family protein"
     gene            3533..3622
                     /gene="kdpF"
                     /locus_tag="I6K64_RS28895"
                     /old_locus_tag="I6K64_28890"
     CDS             3533..3622
                     /gene="kdpF"
                     /locus_tag="I6K64_RS28895"
                     /old_locus_tag="I6K64_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="WP_001272653.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
                     /name="kdpF"
     gene            3622..>5191
                     /gene="kdpA"
                     /locus_tag="I6K64_RS28900"
                     /old_locus_tag="I6K64_28895"
     CDS             3622..>5191
                     /gene="kdpA"
                     /locus_tag="I6K64_RS28900"
                     /old_locus_tag="I6K64_28895"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /GO_component="GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA]"
                     /GO_function="GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="WP_204724566.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVERV
                     LFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLALN
                     TAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSMSTL
                     GNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQ
                     EAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGR
                     MLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSLFAVVTT
                     AASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIG
                     RTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLY
                     AVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQAASS
                     GTLPTH"
                     /name="kdpA"
ORIGIN
        1 cacatgctga gggtgctgtc cgggtgcagc tccgggtcgg gcaggcggtt acctgccggg
       61 tctgtggcat acgggatgcg gatatccaga ttcgctgcgg tggtgtgaac gccggtcagc
      121 ctgccggtgg tgctgtagct gtaactccgg gtctggcgcg ggctgctgat gcggatgagt
      181 tcgccgttgt cgttccaggt gtaatcgcgg tcagacagca ggctgttcag gtgctggctc
      241 tgtaactgcc cggcaggggt ataagcggtg gtgagttcat aacggccgaa gctgcgcagc
      301 gtttcccggt gcaggcggtc gcgggtgtac tccaccagcg gtgtgtcgcc gagtttcatg
      361 cctgccaggt aaccgctgcc gtaggtcagc cattccacgg cgggcaggct gtccggtata
      421 cagcggttcg ccagcccctg cgcgttgtac gcatgtctgg tctcatgctg ccagagcagt
      481 gcttccgtct gcgggtgatg caccgtctga cgctcaccgg tcagccggcc tttctcatca
      541 tatccgtaat acaccgccac ccggtgccct tcgctgatat ggctgatgtc tgtcagccag
      601 ccgcgttcgt cataccgcca ccgctctgcg gtttcaccct tcacggtgcg gtgcgtgagg
      661 cggtctgctt cgtcatagtg ccagtgggtg accagaccct catcctcgct gcggataagt
      721 ttgccggtca gatcgtggtg ataacgctgt gtgcggccgt caaagccggt ttcctgtatc
      781 agccggtcga gtacatcgta acggaaggtg gtgtggctgc cgttttcact ggtcaggcgg
      841 atgacccgtc cggcagcatc gtattccata ctgcgcgtca gcccgccctg cgtggtacag
      901 atggcctttc cccacgcatc gtactgtgtc ccgtttctgc tgccgtccgg ggcaatgacg
      961 gcggtcaggt caccggcgat gttgtattca taccgcgttt catggccctg cgtgtctttc
     1021 acggcaatta actgtccacg gctgtcgtat gcgcggtact gactcagccc ttcctcgcgg
     1081 tgcaccgccg tcatctgccc gaagcggtca tggtcataac gggttacata accggaacag
     1141 tcggtgaagc tcagcaactg accgtaacgg ctccacgtca tggttttccg gctgccggtg
     1201 gcatcttccg ttgcgcaggg taagtcactg tgtggattat cataacggta gcgggtgata
     1261 tcgccgtcag gggcagtttc ctgaatcaga cggcccaatt catcatattc ccggcgtatt
     1321 tccagcccgt caggcccggt ggctgacgtt aactggctgt ggtggttata gtaaaacgcc
     1381 gatgccctgc catccggcgt ggtgatgcgc gtgatgaggc ccgtcaccac atccgggctg
     1441 tattctgttg tccggcctgc ggcatccgtc tgtgtcctga gcctgcccac ggcgtcaaac
     1501 tgactctgcg tgacgctgcc gtccgcgtgt tcctttttca ccacccgctt cagcccggct
     1561 tcgccctgcg tgtgcagcac ttcacggcgg tccaggctgt cggtgatggt gatgtggtct
     1621 ttctcatact gatacgtgta gcttaagcct gccgggttaa gctgttctgt cacccgcccg
     1681 tcgctgtcgt aacggtaacg gatttccggc cggcccgtgt gacggtgcgc caccatccgg
     1741 ccccggtatt tatcatcgta agtaaagctg cgcacctgtt tgccactacg gtcatacacc
     1801 accgccagtt cgccgcgtgg cgtccagcca tagcgcacca gcggcgcggc aggtaaattc
     1861 tccgggtatt ccgggtcgtg cgtcagccac acggcagaca gacggatgcc gttgtcccgg
     1921 ccatattcgg tgtaacccgg cagggtatca ggaaaagcgg acggttccgt cccgccggaa
     1981 atggcctgct gccgggcttc ttctgcccgc tgcgcctgcg tggtcagtac cagccggaag
     2041 tgacgccagg caccatccgt cacgccggtg atttcgccgc tgaattcacc ggcggcttcg
     2101 cggtggaacg tctgtgtgcg cccgaagcgg tccaccagcc cggtcagtac ccggtacggc
     2161 ggcagcggcg caggcagcac ctcatccgct tccggcaccc gctcacacca accgagcagc
     2221 caccacggcc cctgcggact gtttgtcgcc agataacgat gcggacttaa gcggagttct
     2281 tccggcagcg cctgccagag tgcggccagc cggtgacctt catccagttt cgccacgccg
     2341 ccgcgcacca gccacagtga ctcgctgcgg ctgtaaccgt cctcaccggg aaacaggtgc
     2401 tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc gcgcagctgt
     2461 aagcggatat ccgcaggcat tttccagccg gggccgaggc tccccaccgg cgcgggcgtt
     2521 tttgtccggt aactgctgta ggtgcgggag aggatgaacg gcagcgggcc gggcagggcg
     2581 atgtcggttt caccgggaag gacctttgca ccgagcaggg gattgaccgg atggccggac
     2641 gtcactccgc cggggcacac cgaacaggcc acgccggtgg gggcaccaat acgtaccccg
     2701 gctgaaccct gaacaatgct accgccatac tgcgtcatgt caccctgacg cgccgccggt
     2761 tttccgctca tctgctcatc cttgttttat cgggttattt gtctgctgac aggcgaaacc
     2821 ctacagattt ataagaaagc cttaagtgag atatctgtca gataagaaca attaggaaaa
     2881 tatttatata tatttcatga atatatgttt cgattggtat aacgatgtct aaatattcct
     2941 ctgaacatct tcctgataca aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt
     3001 caaaactcag caatcaataa aaatcaccag ttgccttttc ggcctgatcc atccacaccg
     3061 gtttatcgct ggttttcgac cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt
     3121 tccagaaaag catgaaaggc agcgccagaa cgcccgctgc aaccgcataa gtacggcgta
     3181 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
     3241 tctacccggt gaatggcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
     3301 ttttgtgtaa ttttactact catccgacca cttatttttg cttattgatg gtttatttac
     3361 attcatcctg taattaagtt acacaaaagt taaattaata ctaaacatta gttaaatcat
     3421 ggcttttgcc atttttatac tttttttaca ccccgcccgc agatttttgc gaaatctttg
     3481 cagccagaat tctacccttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc
     3541 aggcgtgata accggcgtat tgctggtgtt tttattactg ggttatctgg tttatgccct
     3601 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgatcgc cacgttttta
     3661 ctggtgttaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
     3721 attcctcttc ccggtacaac gggcgttgag cgcgtacttt ttcgcgcact tggcgtctct
     3781 gaccgtgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg
     3841 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag
     3901 ttgccagggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt caccaatacc
     3961 aactggcaat cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcgggctta
     4021 acggtgcaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cctcatccgt
     4081 gcgtttaccc gccagagcat gagcacgctc gggaatgcct gggtcgatct gctacgcatc
     4141 acgttatggg tgctagtccc tgtggcgttg ttgattgcac tgttttttat tcaacaaggt
     4201 gcgctgcaaa actttctgcc ttatcaggct gtgaataccg ttgaaggagc gcaacagctg
     4261 ttacccatgg ggcctgtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt
     4321 ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
     4381 gtgcagatgc tggcgatctt cttgatccca acggcgctgt gctttgcctt tggtgaagtg
     4441 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     4501 tgcgtaggcg tggtgatgtg ggcagaagtt cagggtaatc ctcatctgct ggcactgggc
     4561 acggacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     4621 ctgtttgcgg tcgtgacgac ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg
     4681 tttaccgctc tcggtggcat ggtgccgatg tggctgatgc aaattggtga agtggtgttc
     4741 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     4801 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     4861 atgaaactga ctgcactggc aattctggtt accccgacgc tggtgctgat gggcgcggcg
     4921 ttggcgatga tgaccgacgc cggacgtagc gccatgctca accctggccc gcatggtttt
     4981 agcgaagtgc tgtacgccgt gtcatccgcc gctaacaaca acggcagcgc ctttgccgga
     5041 ttaagcgcca actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     5101 ttcggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa
     5161 gccgccagct ccggcacgct gccaacgcac g
//
