LOCUS       NZ_CP069925             5317 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed16.
ACCESSION   NZ_CP069925
VERSION     NZ_CP069925.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069925.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed16"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            92..658
                     /gene="efp"
                     /locus_tag="I6K64_RS28835"
                     /old_locus_tag="I6K64_28830"
                     /pseudo=""
     CDS             92..658
                     /gene="efp"
                     /locus_tag="I6K64_RS28835"
                     /old_locus_tag="I6K64_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /name="efp"
     gene            710..835
                     /gene="ecnA"
                     /locus_tag="I6K64_RS28840"
                     /old_locus_tag="I6K64_28835"
     CDS             710..835
                     /gene="ecnA"
                     /locus_tag="I6K64_RS28840"
                     /old_locus_tag="I6K64_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="WP_000977757.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
                     /name="ecnA"
     gene            946..1092
                     /gene="ecnB"
                     /locus_tag="I6K64_RS28845"
                     /old_locus_tag="I6K64_28840"
     CDS             946..1092
                     /gene="ecnB"
                     /locus_tag="I6K64_RS28845"
                     /old_locus_tag="I6K64_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="WP_000239596.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAATK
                     AQQ"
                     /name="ecnB"
     gene            1268..1585
                     /gene="sugE"
                     /locus_tag="I6K64_RS28850"
                     /old_locus_tag="I6K64_28845"
     CDS             1268..1585
                     /gene="sugE"
                     /locus_tag="I6K64_RS28850"
                     /old_locus_tag="I6K64_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_000118482.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMAL
                     LAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKLST
                     H"
                     /name="sugE"
     gene            complement(1582..2115)
                     /gene="blc"
                     /locus_tag="I6K64_RS28855"
                     /old_locus_tag="I6K64_28850"
     CDS             complement(1582..2115)
                     /gene="blc"
                     /locus_tag="I6K64_RS28855"
                     /old_locus_tag="I6K64_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="WP_001238378.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWYE
                     IARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRAAL
                     KVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVATREG
                     FDVSKFIWVQQPGS"
                     /name="blc"
     gene            complement(2204..3337)
                     /locus_tag="I6K64_RS28860"
                     /old_locus_tag="I6K64_28855"
     CDS             complement(2204..3337)
                     /locus_tag="I6K64_RS28860"
                     /old_locus_tag="I6K64_28855"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="WP_001336292.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMA
                     VAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDP
                     TTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQ
                     RLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREG
                     KAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDM
                     YQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGS
                     YVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ"
                     /name="BlaEC family class C beta-lactamase"
     gene            complement(3400..3759)
                     /gene="frdD"
                     /locus_tag="I6K64_RS28865"
                     /old_locus_tag="I6K64_28860"
     CDS             complement(3400..3759)
                     /gene="frdD"
                     /locus_tag="I6K64_RS28865"
                     /old_locus_tag="I6K64_28860"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="WP_000609663.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGLF
                     PGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGL
                     AAILTVVTLIGVVTI"
                     /name="frdD"
     gene            complement(3770..4165)
                     /gene="frdC"
                     /locus_tag="I6K64_RS28870"
                     /old_locus_tag="I6K64_28865"
     CDS             complement(3770..4165)
                     /gene="frdC"
                     /locus_tag="I6K64_RS28870"
                     /old_locus_tag="I6K64_28865"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="WP_000208757.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELIF
                     GLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDEKM
                     GPEPIIKSLWAVTVVATIVILFVALYW"
                     /name="frdC"
     gene            complement(4176..4910)
                     /gene="frdB"
                     /locus_tag="I6K64_RS28875"
                     /old_locus_tag="I6K64_28870"
     CDS             complement(4176..4910)
                     /gene="frdB"
                     /locus_tag="I6K64_RS28875"
                     /old_locus_tag="I6K64_28870"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="WP_000829498.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIK
                     DNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLV
                     VDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFG
                     LNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPA
                     AAIQQGKVESSKDFLIATLKPR"
                     /name="frdB"
     gene            complement(4903..>5317)
                     /locus_tag="I6K64_RS28880"
                     /old_locus_tag="I6K64_28875"
                     /pseudo=""
     CDS             complement(4903..>5317)
                     /locus_tag="I6K64_RS28880"
                     /old_locus_tag="I6K64_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; incomplete; too
                     short partial abutting assembly gap; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /name="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
     ARG             complement(2208..3337)
                     /name="blaEC"
                     /gene="blaEC"
                     /gene_name="BlaEC family class C beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2205..3337)
                     /name="Escherichia coli ampC beta-lactamase"
                     /gene="Escherichia coli ampC beta-lactamase"
                     /gene_name="ampC-type beta-lactamase"
                     /tool="rgi"
                     /drug_class="cephalosporin; penam"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gttaaccaat cttctctgga tggggtattt agggttacaa tatgcgccat tttgtggctt
       61 agctaccaat taacaaattt cagagggcct tatggcaacg tactatagca acgattttcg
      121 tgctggtctt acaatcatgt gagacggcga accttacgcg gttgaagcga gtgaattcgt
      181 aaaaccgggt aaaggccagg catttgctcg cgttaaactg cgtcgtctgc tgaccggtac
      241 tcgcgtagaa aaaaccttca aatctactga ttccgctgaa ggcgctgatg ttgtcgatat
      301 gaacctgact tacctgtaca acgacggtga gttctggcac ttcatgaaca acgaaacttt
      361 cgagcagctg tctgctgatg caaaagcaat tggtgacaac gctaaatggc tgctggatca
      421 ggcagagtgc atcgtaactc tgtggaatgg tcagccgatc tccgttactc cgccgaactt
      481 cgttgaactg gaaatcgttg ataccgatcc gggcctgaaa ggtgataccg caggtactgg
      541 tggcaaaccg gctaccctgt ctactggcgc tgtggttaaa gttccgctgt ttgtacaaat
      601 cggcgaagtc atcaaagtgg atacccgctc tggtgaatac gtctctcgcg tgaagtaatg
      661 cggttgtggt gcggcctgca ggctgcacca tcacttattc aggtcagaga tgatgaaacg
      721 ccttatcgtt cttgttttgc ttgccagcac gctgctcacg ggctgtaaca ccgctcgcgg
      781 tttcggcgaa gacatcaaac atctcggcaa ctccatctct cgcgctgcca gctaattttt
      841 cttctcttcc gaaaaatcat cagattccca tcatttttgg cgatgttgtc tattattaat
      901 ttgctatagg caaacataaa taacattacc taaaaggaag acgttatggt gaagaagaca
      961 attgcagcga tcttttctgt tctggtgctt tcaacagtat taactgcctg caacaccacg
     1021 cgtggcgttg gtgaagacat ttctgatggc ggtaacgcga tttctggcgc agcaacgaaa
     1081 gcgcagcaat aagcaataac ggtacgacag ctgtgtcgtg ccgtttgttt tttctgcgat
     1141 agtcacaaag gtaatagttg aaattcccct gccacctggc aaaatatccg ttcaaccatc
     1201 agctttgcag gacgacctgc aaacgcctct tttcaccggg gacggcccca attctccgga
     1261 gcctgatatg tcctggatta tcttagttat tgctggtctg ctggaagtgg tatgggccgt
     1321 tggcctgaaa tatacccacg gctttagtcg tttgacgccg agtgttatta ctgtgacggc
     1381 gatgattgtc agtatggcgc tacttgcctg ggcgatgaaa tcgttaccag tagggacggc
     1441 ttatgccgtg tggacgggta ttggcgcagt cggcgcggcc ataaccggca ttgtgctgct
     1501 cggtgagtcc gctaacccga tgcgcctggc gagtctggcg ttaatcgtat tggggattat
     1561 tggtctgaaa ctcagcactc actaactacc aggctgctgt acccaaataa atttactgac
     1621 atcaaaccct tcccgggtcg cgactgccag catctcctgt ttcacttcgt cagaaatggt
     1681 tggcgtgcgg gagagtatcc acaggtagtc gcggtccggg ccgcaaacca gcgcatggcg
     1741 gtattcccga tcgagtgcaa taacgttata accgccatag aaaggaccaa agaatgacac
     1801 tttcagcgca gcgcgagttg gtgcgccggt aaagtacgct ttcccttcac tctgctgcca
     1861 cattcctctg tcagggttat agcctttatt aatgacattc aggccgccgt catcacgcag
     1921 gctgtatgtt gcggtgactt tttccagtcc acgttcaaag cggtgatcaa aacgggcaat
     1981 ctcataccag gtaccgagat aacgtttggc gtcgaaatta tttactacgg tcacgccacg
     2041 cggcggcgta ggagaactgc aggcaacgac cagaaatgca gctgtcgctg cggcaacgag
     2101 agggagcagg cgcataaatg tttccttact ggtttttttc taagtgtaga tgacagcaag
     2161 gaaaagcgga gaaaaggtcc gaaaattcgg acccgatgga attttactgt agagcgttaa
     2221 gaatctgcca ggcggcgtcg actctcgctg gattgggata gtttttgttt gccagcatca
     2281 cgatacccag ctctttttct ggaataaacg cgacatagct accaaatccg ccggtcgccc
     2341 ctgttttatg tacccatgat gcgcgtactg caggagttgg gggcgtaatc gcttttacgg
     2401 ggcgtgctgc cagtgcaatt ttattgtcac tgccgttaat gatgctgtca ggatttaccg
     2461 gccagtccag catttcccag cccaggccct gatacatatc gccggtttgc cagtagcgag
     2521 attgtgccag ttgtatccct tgttgaagcg ttttctcatt gatatcaagg ggttttaaat
     2581 tgctttgcac ccagcgggcc atatcttcaa tagtcgactt cacaccataa gcttcagcat
     2641 ctaacgcccc aggcgaaaca tgcactgcct taccttcgcg atatccccag gcgtaattct
     2701 tttcttctgc gggcggtaca ttaatccacg tatggttgag tttgagtggc tggaagacac
     2761 gagtttgcat cgcctgctca aaactcaaac cagacggctt cacagccagt gcgccgaaca
     2821 aaccgatact ggagttggca tacagacgtt gtgttcctgg agcccatgca ggctgccagt
     2881 tttgatagaa gcgcagcaag tcgcttgagg atttcacctc atccggcacc tgcaatggca
     2941 ggccgccagc agtgtaggtt gcgagatgta atagtgtgat cccattccac tgtttagcgg
     3001 taagttcagg ccagtatttt gttgtgggat cgcttaactt gatttcccct cgagcaatag
     3061 cgtcgccacc aagcacgcca gtaaatgttt tgctgaccga acctaactca aacaacgttt
     3121 gctgtgtgac gggctgcttt ttggcgatgt ccgcatagcc ccaggtaaag taataaggtt
     3181 taccctgata aattaccgcc accgccatac ccgggatctt ttgttgctct ataagcgggg
     3241 taattgtgcg atgcacaata tcgttgattt gttgaggggc agcaaatgtg gagcaagagg
     3301 cggtaattaa taaggcgcag agcgtcgttt tgaacatagg gtctggtttc catacaaaac
     3361 ggcccgccat aggcgggccg gatttacatt ggcgatgcgt tagattgtaa cgacaccaat
     3421 cagcgtgaca actgtcagga tagcagccag accgtagaaa acccatttgc ccgcaggtac
     3481 gtggattttc agatcgtgca tcgcgtggtg catacggtgt aaaccacacc acagcggcag
     3541 aacgatcatc aggaacagga atacgcgacc aatgaagctc tgcgcgaacg ccagaacgcg
     3601 ctcgtagctc agcgcatcac ccggaaacaa ccccagtggc agcagaatac ccaccagcag
     3661 gatcatcacc ggcgcaatga tggcgctcca cataccaccg gccccgaaga ggccccagaa
     3721 taccggttcg tcagaacgct ttggatttgg attaatcatc tcaggctcct taccagtaca
     3781 gggcaacaaa caggattacg atggtggcaa ccacagttac cgcccagaga cttttgataa
     3841 ttggctctgg tcccattttt tcgtctttta caatgatatt ggccgctttc ggtgccagtt
     3901 caaaccaggt tttggtgtgc agcagagctg ccgccagagt gatcaggtta atgatcacga
     3961 taaccgggtt ttgtaaaaag tcgacgaatc ccgcccaggc ttccgggcca tttttcaggg
     4021 caaacagccc gaaaatcagt tcaatgctga accacacagc cggaaccgcc gtgccttcgc
     4081 gcagcatgta aaagcgataa aacggcaatt ttttccacca ggtggacgtc attggccgta
     4141 catacggttt acgtttagtc gtcatgttgc actccttagc gtggtttcag ggtcgcgata
     4201 agaaagtctt tcgaactttc tactttgccc tgctgaatgg ccgcagccgg atcgacgtgt
     4261 ttcgggcaga cttcggagca gtagcccacg aaagtacagc tccatacgcc gttctggctg
     4321 ttcaactgcg ccatacgctc cttcttaccg tggtcgcggc tatcttcgtt ataacgatgc
     4381 gccagcgtaa tggcagccgg accgatgaac tctgggttca ggccaaactg cgggcacgcg
     4441 gcgtagcaca aaccacagtt gatgcaaccg gagaactggt gatacttcgc catctgcgcc
     4501 ggggtctgga tgttagtacc ctgatccgcg gtgcgggagt tgccgatgat gtacggtttg
     4561 atcgcttcca gactttcgat gaagtgggtc atatcgacca ccagatcgcg ttcaatcggg
     4621 aagttagcta acgcttcaac cttcataccg tcggtgtaat cacgcaggaa ggttttacat
     4681 gccagttttg gcacgttgtt aaccatcatg ccgcaggaac cacaaatcgc catacggcag
     4741 gaccagcggt agctcaggtc cggtgccagg ttgtctttga tgtagcccag cgcatccagt
     4801 aatgaggtag ttgcgtcata aggcacttca tagaatgcgc tatgcggtgc ggtatcgact
     4861 tccgggttat agcgcaccac ctcaattttc aggtttttca tctcagccat tcgccttctc
     4921 cttcttattg gctgcttccg ccttatcggc tgcatccgct tcgccaccgt aaacgcgttt
     4981 agctggcggc agcgtagtaa tcttcacgtc gctgtactcc aggcgagtcg tgccatcagc
     5041 atcgcggaag gcgagggtgt gtttgaggaa gttgacgtcg tcacgctcgg tgcaaccttc
     5101 gtccagacgc tggtgcgcgc cgcgggactc tttacgtgcc attgcggagt gcgccataca
     5161 ttcagcaacg ttcagaccgt ggcccagttc aatggtgtag agcacggcgg ccgcggacac
     5221 gccggaagcg ccagcgctcc gcccccccgt gacgcgcccc cgcagccccg ccagcccgcc
     5281 cacggcctcc cgcaccagcc ccgccgtccg ccagaca
//
