LOCUS       NZ_CP069924             5374 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed15.
ACCESSION   NZ_CP069924
VERSION     NZ_CP069924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069924.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed15"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..429
                     /locus_tag="I6K64_RS28800"
                     /old_locus_tag="I6K64_28795"
     CDS             <1..429
                     /locus_tag="I6K64_RS28800"
                     /old_locus_tag="I6K64_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="WP_265105074.1"
                     /translation="EPTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPK
                     AAGIAMALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHG
                     DYINDKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
                     /name="acetyltransferase"
     gene            complement(470..1900)
                     /locus_tag="I6K64_RS28805"
                     /old_locus_tag="I6K64_28800"
     CDS             complement(470..1900)
                     /locus_tag="I6K64_RS28805"
                     /old_locus_tag="I6K64_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="WP_001333507.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKK
                     GEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITM
                     LMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEY
                     GKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKF
                     PVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQ
                     LGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLP
                     LLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVT
                     ETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFN
                     GQEMSEEEFMSRAGRFVH"
                     /name="YdgA family protein"
     gene            complement(2054..2680)
                     /gene="dsbA"
                     /locus_tag="I6K64_RS28810"
                     /old_locus_tag="I6K64_28805"
     CDS             complement(2054..2680)
                     /gene="dsbA"
                     /locus_tag="I6K64_RS28810"
                     /old_locus_tag="I6K64_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="WP_000725337.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEFF
                     SFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALGVE
                     DKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKAAAD
                     VQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
                     /name="dsbA"
     gene            complement(2697..3683)
                     /gene="srkA"
                     /locus_tag="I6K64_RS28815"
                     /old_locus_tag="I6K64_28810"
     CDS             complement(2697..3683)
                     /gene="srkA"
                     /locus_tag="I6K64_RS28815"
                     /old_locus_tag="I6K64_28810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="WP_001065497.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQFQ
                     DEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFA
                     VFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDA
                     TLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDAR
                     NGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWL
                     MRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
                     /name="srkA"
     gene            complement(3760..4029)
                     /locus_tag="I6K64_RS28820"
                     /old_locus_tag="I6K64_28815"
     CDS             complement(3760..4029)
                     /locus_tag="I6K64_RS28820"
                     /old_locus_tag="I6K64_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="WP_001295263.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELADL
                     TDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
                     /name="YihD family protein"
     gene            4099..4683
                     /gene="mobA"
                     /locus_tag="I6K64_RS28825"
                     /old_locus_tag="I6K64_28820"
     CDS             4099..4683
                     /gene="mobA"
                     /locus_tag="I6K64_RS28825"
                     /old_locus_tag="I6K64_28820"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0061603 - molybdenum cofactor guanylyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="WP_001052181.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALMT
                     QLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDTPY
                     IPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVFMRL
                     AGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
                     /name="mobA"
     gene            4665..5192
                     /gene="mobB"
                     /locus_tag="I6K64_RS28830"
                     /old_locus_tag="I6K64_28825"
     CDS             4665..5192
                     /gene="mobB"
                     /locus_tag="I6K64_RS28830"
                     /old_locus_tag="I6K64_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="WP_000907622.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHTH
                     HDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKLDL
                     ILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVEGLA
                     DFVVEWMQKQNG"
                     /name="mobB"
ORIGIN
        1 gaacccactc ggcgttcttg tgaaaagttt cgcctgcatc ccaccaccat tgtcaatttc
       61 gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa cccactccac ttttcaaaac
      121 ttgttgccac caaaggcggc aggcattgcg atggcgctaa atgtactggg taaacaattc
      181 gataaactgc ttaatgttac gctgtgttat ccggacaata accgtcagcc gttcttcgat
      241 atgttaagcg gtaaactgac gcggattgtc gtacatgtgg atttacagcc tattgccgat
      301 gagttacatg gcgactacat caatgacaaa agctttaagc gccattttca gcaatggctg
      361 aactcattgt ggcaggaaaa agaccggctt ctgacgtctc tcatgtcatc acagcgtcag
      421 aataagtaag ttttactcgc ctctgaatga gcagaggcga gtgagtattt taatgaacaa
      481 aacgtccggc acgagacata aattcttctt cgctcatctc ctgtccgtta aaaacaactt
      541 tacccggtgt ataacgcagc tgcagcgagg cggtattgtt gtcaatcgtg attaactgaa
      601 acatctgacc taatgtcatc atcccgctga tttgtttatc agcctgcttt tgcgcttttt
      661 ccgcatccat tccttcagat aaattaagct gttttgcggt ttctgtgacg acattaagcg
      721 gtaacttcac atcaaaattg agcgatttga tatctttgtt tgtggatgat gaagatttgg
      781 ctgggtcggc aatactgata tccagattgg catttagttc gccgagtgcg ttcttccatc
      841 ttaccggttg tttaatggtc ggctcacttt tttgtaataa cggtaaatac tctttgaaca
      901 atgcagcatt cacttcttgc agagcaactt catcgtttgc taactcaggg tgtgcagcaa
      961 gctgcttctg catcgcaatg ttatactgga tgataaattg gcgcactgct gagggatcaa
     1021 tagattcagc aatcaatgac cactcaccac taccgagttg ctgattccct tttgttaact
     1081 tatcaagttc gtaggtgaga ttgacattga tataatcttt gtctttaacg cgatccagcc
     1141 tggtttttgc aacgaaagca tcgatttggg caacgtcttc cccgtgattg ataatgttaa
     1201 tctgattaaa tttcgactcg ctttcaccaa ccggaaattt cttttcttct aatcgcgcca
     1261 gagaatcaat ggtaaaactt tttgccgtta ctttgttcat tggtgataat tgcagaacaa
     1321 tatcttcgac tttgccttca atatccaggt tagacaatga actaccatca ccattaaaag
     1381 taaactggcc ctcaccaaaa gaaaactttc catactctgc aggggcaaca tctaatgtcg
     1441 tagagaatga gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat ttatttttcg
     1501 cggcgataaa tagcggttgt gtcagttcat tattcactaa gttcactttt gccgctgcca
     1561 gtgctgggat gacattacca tgcattaaca ttgtgatggg caacggaccg tgctcaacat
     1621 ccacatcaaa tacaactttc tggcctggct tgatattgag atcgggtgca ccgttatcga
     1681 atgtcatttg catttgaaaa cgggaattaa aaaagccttt gtcaaaattt tgataactta
     1741 acgtcatatc gtaggcatgt tcacctttct ttttagcatc gttaaaatct ttaataaatt
     1801 tttcgacacc aggctgaatt tgcgtacctg tgtaccatgt gccaccaccc cagattacgg
     1861 ctaacgcaac aataacacct gtagctgact tacgtatcat ataaatattc cctgataaaa
     1921 cagcaaaact ttgaatatcc acttatgctg aaataatgtc cagcggcagg atgcattatc
     1981 agaatgtatt tatatttatt aaataataaa aaagcccgtg aatattcacg ggctttatgt
     2041 aatttacatt gaattatttt ttctcggaca gatatttcac tgtatcagca tactgctgaa
     2101 caaaaacatc catattgctg gtatccatac cctgcggatt cagctgatat ttaccgttaa
     2161 caaacatcgc cggaacgcca cgcaattgca cgtcagctgc agctttttcc tgctgagcga
     2221 ccagagattt caccacgaag ctgttccacg ccgcgtcgta ctcttcacct ttaatacctg
     2281 cgttgataaa tacatcgcgg atatcagaag cagaacgaat ggtctgggtt ttctgtacgc
     2341 cttcaaacag cggaacagtc actttgtctt ccacgcccag cgccatcgcc acagcccatg
     2401 cctgagtcag atctttgccc aggtcaccac ccatgaagtt gacgtggtat ttagtcatct
     2461 tcacgccttc cggcagtttt ttcttcacat tatcagaaat atgcagaact tcttcaaact
     2521 gatagcagtg cgggcagaag aaagagaaaa actccagcac ttgcggcgcg ccagctaccg
     2581 gtttttccag ggtagtgtac tgtttaccat cttcatactg cgccgccgat gcgctaaacg
     2641 ctaaaactaa accagccagc gccagccaaa tctttttcat gatctactct ctccgattaa
     2701 tacataggtg ttaattgcaa agggggttct tgtagaactt ttgcctgttc tataaaagtc
     2761 gccgtctgtc gcaaccagta atcttccccg gttaaccacg ggaaattttt cgggaacgcg
     2821 ggatcagccc aacgccgcat tagccaggca agataataaa ccaaacgcat ggcgcgtaaa
     2881 ggttcaatca gtccgatttc agcggtgtcg aactcgctaa attcttcata agcttcaata
     2941 atagtttcca gttgcatccg ctgctcggct ttatcgccat tgagcaacat ccacaaatcc
     3001 tgaacggctg gaccattacg tgcatcatcc agatcaacaa acattggacc atcgcgccag
     3061 agaatattcc cggcgtggca gtctccatgt agccgcagaa cggtgaaatc ttcccgccag
     3121 tgtgctgtaa cggcggcaat cagctcatct gtcgctttca ggaatgccgc tttcaacccg
     3181 gaaggtatca gtgtagcgtc ctcaaacagc ttgcgtggct caatgagata ttcgttcaaa
     3241 ccgatggtcg ggcgatggat aaaaagctgt ttgcgccccg tctggtgcat acgccctaaa
     3301 taacgcccaa ccgcttccat ctgatcgata ttatcagctt cgaactggcg accaccgacg
     3361 cttggaaaaa cagcgaaata aaatccctga tgattcaata aagtctgacc gttaaaggcc
     3421 acaggtgctg cgaccggaac ttcatcattt accagctgca acgcaaattg atgttcttcg
     3481 aggatttgat cggctgtcca acgttcaggg cgataaaatt tgacgacaaa acgtcgacga
     3541 tcttcgtcct gaaattgata gacacggttt tcatagctgt taagcggggt aagaccggaa
     3601 tccacccgga tcccatgctc aaacagagcg tccatgatgg tatccgggtg tagtgtctgg
     3661 aaagtaaaag cgctgttatt catcccatca tccggaaaac attacgaatg acgaaggata
     3721 tcatttcacg atgattttgg tggcgccgct tacaagcttt tactctttaa ttacgccgcg
     3781 agcgcgtaac agcgccgttt tgaagtcttc ttcataatct ttctgcaaac ccgggatcac
     3841 cgcatctttc gcggaatcac gcattttcag gtgatagatc agaatgtcat ccgtcaaatc
     3901 cgccagttcg ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt gcagcaggtt
     3961 aaggtctggc tctttttgcc aggctggctg gaggagttca ataacttcat tcagacgttt
     4021 acatttcata gtgatgctcc ttactcttga gacagacacg ttagcagggt caatcccaca
     4081 ataaaagagg cgatatcggt gaatctgatg acgacgataa caggcgttgt gctggcaggc
     4141 ggtaaagcca gacgaatggg cggcgtagat aaaggattgc ttgaattaaa cggcaaacca
     4201 ttatggcaac atgtcgctga cgcgcttatg acgcagctct ctcacgtcgt ggttaatgct
     4261 aatcgtcatc aggaaatcta tcaggcaagc ggtctgaaag tgattgaaga ttcactggcg
     4321 gattacccag gccctctggc aggaatgctt tcagtaatgc agcaggaagc tggtgagtgg
     4381 tttttgtttt gcccgtgcga tacgccttac attccccctg atttagcagc caggcttaat
     4441 catcagcgca aagatgcgcc tgtcgtgtgg gtccatgacg gtgaacgcga tcacccgact
     4501 attgctctgg taaaccgcgc tattgagcct ttattactgg aatatctgca agcaggagaa
     4561 cgccgggtaa tggtatttat gcgtctggct ggcggtcatg cggttgattt cagcgatcat
     4621 aaagatgcat ttgttaacgt gaatacgcca gaggagcttg cccgatggca ggaaaaacga
     4681 tgataccgtt actcgccttt gccgcgtgga gtggtaccgg aaaaactacg cttttgaaaa
     4741 aactgatccc ggcattatgc gccagaggga tccgtccagg gctgattaag catacgcacc
     4801 atgatatgga tgttgataag ccaggcaaag atagctatga gctgcgcaag gctggcgcgg
     4861 cacaaaccat cgttgccagc cagcaacgat gggccttgat gacggaaaca ccagacgaag
     4921 aagagctgga tctacagttt ctcgcaagtc gaatggatac ctcaaagctg gatttgattc
     4981 tggtcgaagg gtttaagcat gaagagatcg caaagattgt gctgtttcgc gatggagccg
     5041 gacatcgacc tgaagaatta gtgatagaca ggcatgttat tgctgtagcc agtgatgtgc
     5101 cgcttaatct tgatgtcgca ttactggata ttaatgatgt tgaggggctg gctgattttg
     5161 tggtggagtg gatgcaaaag cagaacggat aagcaagagt aggctggata agacgcgcca
     5221 gcgtcgcatc cggcattttt ttctgcgcca atgcaaaaag gccatccgtc aggatggcct
     5281 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     5341 gttgcttcgc aacgttcaaa tccgctcccg gcgg
//
