LOCUS       NZ_CP069920             9520 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed9.
ACCESSION   NZ_CP069920
VERSION     NZ_CP069920.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069920.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..210
                     /locus_tag="I6K64_RS28590"
                     /old_locus_tag="I6K64_28585"
     CDS             <1..210
                     /locus_tag="I6K64_RS28590"
                     /old_locus_tag="I6K64_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="WP_223724605.1"
                     /translation="RTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEACRRRLCSEK
                     RVSSEANKEKSDITELLRKQVRPD"
                     /name="YlcI/YnfO family protein"
     gene            268..678
                     /locus_tag="I6K64_RS28595"
                     /old_locus_tag="I6K64_28590"
     CDS             268..678
                     /locus_tag="I6K64_RS28595"
                     /old_locus_tag="I6K64_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="WP_000373090.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENVH
                     IVLKNDNTVLLKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFRWV
                     TNIHEQKRYYYTFDNSLLFTESIQKTTQILPR"
                     /name="DUF1398 domain-containing protein"
     gene            complement(830..1003)
                     /locus_tag="I6K64_RS28600"
                     /old_locus_tag="I6K64_28595"
     CDS             complement(830..1003)
                     /locus_tag="I6K64_RS28600"
                     /old_locus_tag="I6K64_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="WP_001019606.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKMT
                     GLESYDVKINLI"
                     /name="addiction module toxin, GnsA/GnsB family"
     gene            complement(1175..1405)
                     /gene="ynfN"
                     /locus_tag="I6K64_RS28605"
                     /old_locus_tag="I6K64_28600"
     CDS             complement(1175..1405)
                     /gene="ynfN"
                     /locus_tag="I6K64_RS28605"
                     /old_locus_tag="I6K64_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="WP_000720962.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGFP
                     SLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
                     /name="ynfN"
     gene            complement(1410..1475)
                     /gene="ynfR"
                     /locus_tag="I6K64_RS28610"
                     /old_locus_tag="I6K64_28605"
     CDS             complement(1410..1475)
                     /gene="ynfR"
                     /locus_tag="I6K64_RS28610"
                     /old_locus_tag="I6K64_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="WP_120795388.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
                     /name="ynfR"
     gene            complement(1478..1666)
                     /locus_tag="I6K64_RS28615"
                     /old_locus_tag="I6K64_28610"
     CDS             complement(1478..1666)
                     /locus_tag="I6K64_RS28615"
                     /old_locus_tag="I6K64_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_071524604.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITFD
                     NISQYLNASRLSLDLKK"
                     /name="cold-shock protein"
     gene            complement(1677..1889)
                     /gene="cspI"
                     /locus_tag="I6K64_RS28620"
                     /old_locus_tag="I6K64_28615"
     CDS             complement(1677..1889)
                     /gene="cspI"
                     /locus_tag="I6K64_RS28620"
                     /old_locus_tag="I6K64_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="WP_000066495.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKTL
                     TENQEVEFGIENGPKGPAAVHVVAL"
                     /name="cspI"
     gene            complement(2252..2749)
                     /locus_tag="I6K64_RS28625"
                     /old_locus_tag="I6K64_28620"
     CDS             complement(2252..2749)
                     /locus_tag="I6K64_RS28625"
                     /old_locus_tag="I6K64_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="WP_001071769.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNAQ
                     FAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAKHT
                     ADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRDKKH
                     QM"
                     /name="DUF2514 domain-containing protein"
     gene            complement(2746..3279)
                     /locus_tag="I6K64_RS28630"
                     /old_locus_tag="I6K64_28625"
     CDS             complement(2746..3279)
                     /locus_tag="I6K64_RS28630"
                     /old_locus_tag="I6K64_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="WP_001092971.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRDG
                     SGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQKAG
                     IASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVIR
                     RDQESALTCWGIEQ"
                     /name="lysozyme"
     gene            complement(3276..3587)
                     /locus_tag="I6K64_RS28635"
                     /old_locus_tag="I6K64_28630"
     CDS             complement(3276..3587)
                     /locus_tag="I6K64_RS28635"
                     /old_locus_tag="I6K64_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="WP_000189916.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDRV
                     EGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKYL"
                     /name="YdfR family protein"
     gene            complement(3592..3807)
                     /gene="essD"
                     /locus_tag="I6K64_RS28640"
                     /old_locus_tag="I6K64_28635"
     CDS             complement(3592..3807)
                     /gene="essD"
                     /locus_tag="I6K64_RS28640"
                     /old_locus_tag="I6K64_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="WP_000839590.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGSL
                     VFGLLTYLTNLYFKIKEDRRKAARGE"
                     /name="essD"
     gene            4027..4197
                     /gene="ynfU"
                     /locus_tag="I6K64_RS29665"
     CDS             4027..4197
                     /gene="ynfU"
                     /locus_tag="I6K64_RS29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="WP_211180497.1"
                     /translation="MSERKNSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHCN
                     KVFQTNLKAVA"
                     /name="ynfU"
     gene            complement(4561..4776)
                     /gene="cspB"
                     /locus_tag="I6K64_RS28645"
                     /old_locus_tag="I6K64_28640"
     CDS             complement(4561..4776)
                     /gene="cspB"
                     /locus_tag="I6K64_RS28645"
                     /old_locus_tag="I6K64_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="WP_000066484.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRTL
                     FEGQKVTFSIESGAKGPAAANVIITD"
                     /name="cspB"
     gene            5077..5289
                     /gene="cspF"
                     /locus_tag="I6K64_RS28650"
                     /old_locus_tag="I6K64_28645"
     CDS             5077..5289
                     /gene="cspF"
                     /locus_tag="I6K64_RS28650"
                     /old_locus_tag="I6K64_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="WP_000087756.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEEI
                     TTGLRVEFCRINGLRGPSAANVYLS"
                     /name="cspF"
     gene            5344..5433
                     /locus_tag="I6K64_RS28655"
                     /old_locus_tag="I6K64_28650"
     CDS             5344..5433
                     /locus_tag="I6K64_RS28655"
                     /old_locus_tag="I6K64_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_120795389.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
                     /name="hypothetical protein"
     gene            complement(5711..6463)
                     /locus_tag="I6K64_RS28660"
                     /old_locus_tag="I6K64_28655"
     CDS             complement(5711..6463)
                     /locus_tag="I6K64_RS28660"
                     /old_locus_tag="I6K64_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="WP_001047135.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSKA
                     PLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFADYS
                     RSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTACRG
                     CKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQWYKG
                     YADVIGKLVTKCWQEEAYAEAQLRKVTR"
                     /name="antitermination protein"
     gene            complement(6477..7526)
                     /locus_tag="I6K64_RS28665"
                     /old_locus_tag="I6K64_28660"
     CDS             complement(6477..7526)
                     /locus_tag="I6K64_RS28665"
                     /old_locus_tag="I6K64_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="WP_001265199.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRNL
                     PSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHHTE
                     TVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERELSL
                     AELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQRTKNL
                     APLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPCACCGM
                     PADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLELIFRFID
                     RALTIGVLA"
                     /name="DUF968 domain-containing protein"
     gene            complement(7528..7806)
                     /locus_tag="I6K64_RS28670"
                     /old_locus_tag="I6K64_28665"
     CDS             complement(7528..7806)
                     /locus_tag="I6K64_RS28670"
                     /old_locus_tag="I6K64_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032351280.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEWL
                     IHSYRRRGFEVKKALSLDYRHWIISVRLPYSGRPPRASRTFQQRIWR"
                     /name="hypothetical protein"
     gene            complement(7873..8124)
                     /gene="rem"
                     /locus_tag="I6K64_RS28675"
                     /old_locus_tag="I6K64_28670"
     CDS             complement(7873..8124)
                     /gene="rem"
                     /locus_tag="I6K64_RS28675"
                     /old_locus_tag="I6K64_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="WP_204724562.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIVCLRKNGAALINF
                     VDARVTDGFILREGEFVTSLQVLKEIGIKAGFSAFSGE"
                     /name="rem"
     gene            complement(8341..8496)
                     /gene="hokD"
                     /locus_tag="I6K64_RS28680"
                     /old_locus_tag="I6K64_28675"
     CDS             complement(8341..8496)
                     /gene="hokD"
                     /locus_tag="I6K64_RS28680"
                     /old_locus_tag="I6K64_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="WP_000813254.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFT
                     AYEPEE"
                     /name="hokD"
     gene            complement(9055..9476)
                     /locus_tag="I6K64_RS28685"
                     /old_locus_tag="I6K64_28680"
                     /pseudo=""
     CDS             complement(9055..9476)
                     /locus_tag="I6K64_RS28685"
                     /old_locus_tag="I6K64_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
ORIGIN
        1 agaacaacaa cccgcaacat ccgctttcct aaccaaatga ttgaacaaat taacatcgct
       61 cttgagcaaa aagggtccgg gaatttctca gcctgggtca ttgaagcctg ccgccggaga
      121 ctgtgctcag aaaaaagagt ttcttctgaa gcaaacaaag aaaagagtga cattactgaa
      181 ttgctcagaa aacaggtcag accagattga agcaatttag ataatcgtgc agactacgcc
      241 ccctcatatc acatggaagg tttatctatg gatcaggtag tcatttttaa acaaatattt
      301 gataaagttc gaaacgattt aaactatcaa tggttttatt ctgagctaaa acgtcacaat
      361 gtctcacatt acatttacta tttagccaca gagaatgttc atattgtatt aaaaaatgat
      421 aatacagtgt tattaaaggg cctaaaaaac attgtgtctg tcaaattttc aaaggatagg
      481 catcttatag aaacgacctc taataagctg aaatccagag agatcacatt tcaggaatac
      541 agaagaaacc ttgctaaagc aggagttttt cggtgggtta caaatatcca cgaacaaaaa
      601 agatattact atacctttga taattcatta ctatttactg aaagcatcca gaaaactaca
      661 cagatcttac cacgctaaac cataacgtcc ggcttctctc actcctgagc cggactgcat
      721 tggtttaata aaaaccatca acaattgtga tttagatatt cggaaccatt caaatataac
      781 aaaaccccgt aaaaacgagg tttatggata aattttatta ttgaatacat cagattaaat
      841 taatcttgac atcatagctt tcaagacccg tcattttttc ccgtgcggta aactgaatac
      901 tggtaacttc tttcccggtc tttttcttaa gttcaataat tttttttgtt atatattcag
      961 aaatatctgc ttctgctttt gtttttaagt tttcaatatt catcatttcc tcttttagtc
     1021 tgttatgact ttccagttac acagtaagtc gattatatgg tgcaaacgtg taaaagataa
     1081 gatgaaacat cgcaataatc aacatacgat agtctaaatt ttacacaaac agacaaagag
     1141 aattttcctg aattatcaat gcaatagcat caaatcaact caagagcctt attgctgctt
     1201 ccagaatttc ttctgaagta acatgtcgat ccgcggctac ataaatgact ttatgatctc
     1261 cggtcagaga tggaaaccct gcggccatta cagtaaggtg tgttttttcg ccatttggat
     1321 attcacgcat gatggtgtta actccagtca tcgctggcac taccactgct ggttcagagt
     1381 taaaaaaaac tatgattttt ttcatgatgt taccgtagta tgtgagtatc catcgaatag
     1441 acaccaagca aaaaagctcc cgaaggagcc ttcattttca cttttttaaa tccaacgaca
     1501 gacggctggc atttaagtat tgtgaaatat tatcaaatgt aatcatcatt gatttacaaa
     1561 agatacattt tgccccgaaa ggattcatgt cagaaacatc aaaagatgat gttctatact
     1621 gggaaccatg acaacacggg catctaaagt gaatatggtt tgtaatattg tctacctcaa
     1681 agcgccacta catgaacagc ggcaggacct ttaggtccgt tctcaatacc aaattcaact
     1741 tcctgattct cagttaatgt tttgaaatcg ttgctctgaa ttgctgagaa atggacaaac
     1801 acatctttgc tgccatcttt cggcgtgatg aaaccaaaac ctttttcagg gttaaaccat
     1861 ttcactaaac cagtcatttt gttagacata attattacct tttgaagaaa ttagcccttg
     1921 ggcagaatgg tccgaaaaaa aatatcagag agaaaaacca acaaggaaat ctcaagaggt
     1981 acaaataata aaattataac aatgactgct tcagataaat ttgtaacaaa ccagaacacc
     2041 attaacgcat gattaaccac ccatagcaag gattactttt gtaaagaaaa acacagcaat
     2101 gaaagaatag ctttatttat taataaaacg tgtcattctg attaagacct tttatcttac
     2161 ccttaagatt tcaggaattt tggctcatgg aagagtcctt tttatttaaa ttttacattc
     2221 cgcgatgtaa atgttccgat ttaatattac cctacatttg atgcttttta tctcttaaag
     2281 attcatagat ctgttgacaa gtcactcctg cgatgtagcg ttcgtcagca atttcagcat
     2341 aaagctgagc ttctgctgca atatctccga gcatgttggt gagcattcct tcggcggttt
     2401 tggttgtttt gcctctgacg gcagcggcaa gatctgcggt atgcttcgct gcgtcaaggc
     2461 gtatggcata tttttttgct tcggcacgca actggttaac actatcagac agataagcag
     2521 ccctggcaga aatttcagca gatttctgtt gcgcatcttt aacagcctca tcacgggcta
     2581 tagttcgccc ctgttcaatt attcgagcag caaattgagc atttacctct tgtgataatg
     2641 cggcagcatc acgttccgcc catttttttt gccatcctcg gtcgctccag acatttccga
     2701 cgataaatcc tgacaacacg agaaaaatca ccatgaatat ctgattcact gttctatccc
     2761 ccagcaggtt aatgcgctct cctggtcacg acgaataacc tgaccgtaac agttatttga
     2821 acgaatgcgg caatcgcgtc cgccatcctt aatccaccag cgaatcgctt cgcatgcacc
     2881 tttacgatca ccagcattca gccgcttata aaacgtcgac gggaaacact taccggggcc
     2941 aatgttatag ggacaaaatg acgcgatacc cgctttttgt ggttcggtca gtggtacttt
     3001 aatattgcgc tccacccatg ccagcgcctt atcacgctca atggcgttga cctggtcgca
     3061 tttttccttc gacagtttca tattgggaaa aacggttttt ccatccacca ctgtggcacc
     3121 ccgacagatg gtccatatgc cagaaccatc gcggtatgcc attgtgtggt taccttcttt
     3181 ttcgtccaga aactggtcaa gtatctgagg agcagatgcg ccagcaccaa tcagcgccag
     3241 aacggcagcc gacaggccgt atctgatttt tgtgttcata gatatttatg atgaggacgc
     3301 tcgtgcttat tggcaggatt ttcaatctta aaggagtact gatgctgcag ataagactca
     3361 actttttctg acaatttttc tgctacttcc aggaagactt gccggacgct ccttctggct
     3421 gctgcctcat aaaactccag cgcagctcct tcaacacggt ccatggcgac atccaggtca
     3481 aaaatttcac cgtcaaagcg ttctttgtcc tgtaaggcta cagttaccgt aactttattc
     3541 tcaaaattac ggactccttt cacaaccagt tcatagtctt gagtcattgg attactctcc
     3601 tctcgcagcc ttacgcctgt cttctttaat cttgaaataa agatttgtca gatacgtcag
     3661 caggccaaaa accaggctac ccagcacacc gattgcagcc cactgtgacg gagttacttt
     3721 atcgagtaac tgcaatgccc agaaaccagc attacccgcc gatgtgccat aggcaacacc
     3781 tgttgttaac ttatccattg atttcatatc ctcaccccga tgtacacgga tggtgcaata
     3841 tgtttgaaaa gatcggagtc tacggggtag ttttgacagc acacgttgtt ctcaacggcg
     3901 ctaaaaaaac atacacatta aaaatgtggg taattatttt gaaagaaagt catatataaa
     3961 ataataatac gagaaatgtt ttcatattta gtgtactgta tacggccatt tatacaggaa
     4021 aagcctatgt cagaacgtaa aaactcaaaa tcacgccgta attatctcgt taaatgttcc
     4081 tgcccaaact gcacccaaga gtcagaacac agtttttcaa gagtacaaaa aggtgccctt
     4141 ttgatctgcc ctcattgcaa caaagtattc cagacaaatc ttaaagctgt agcctgattg
     4201 attttattag taacaagtat tttttatatt ttaataatat atttaaagca gataataaaa
     4261 aacccgcctg agcgggtttg agattgtggt gctttttgtg ggagtcatcc acttacgcac
     4321 tttgttttgc catgccagca gttagcttct gctgtaaaac tattcatgca gcaaacctgc
     4381 acttcaccac aatggttagc atacttttcc tgattaagat tttgccaaat atgctagcca
     4441 ttgtttcatg tattggacct ccttactttt tattaaagag atccaatatt cactactctg
     4501 tccgtatctc tactcaggca tcagccttct tcgttatcgt atacagacga gcgatgaatt
     4561 ttaatcagta atgatgacat ttgctgctgc aggaccttta gcaccactct ctatagagaa
     4621 ggtaaccttt tgaccttcaa ataaggttcg ataattatca ttctgaatcg cagaaaaatg
     4681 cacaaacaca tctttactac catcaacagg agaaataaag ccgaaacctt tatcagcgtt
     4741 aaaccatttt actaaaccag tcattttatt tgacattcta cattccttaa cttgagcctt
     4801 tcggcataaa tggtttgcat aacagaaacg acttcgtact taattggaga gactcaaaga
     4861 aggaataagt gaataacacc tgaaatgaga actgctttag taaactactt cgtatatcgt
     4921 ctgttcttca aaccgacgca atcattaacg catagttgaa catatgaagc aatgtttatt
     4981 ttagacatcc agccatcttc aaccccatca aaaaactata gctttcttca ggaacgtgtg
     5041 tatagtgcgc caagttatca gtattaagga atttttttgt cccgtaaaat gacaggaatt
     5101 gtcaaaacct ttgacggcaa aagcggcaag ggtcttatca ccccatccga tggtcgtatc
     5161 gatgtccagc ttcatgtttc agcgctcaat ctccgcgatg cagaagaaat taccaccgga
     5221 ttacgcgtgg aattttgccg gataaatggt ctgcgtggcc cttcagctgc caatgtttac
     5281 ctttcatgag ctatattaaa gctttaattt caggccccat cggatcacac atggagagtt
     5341 tttatgaata accccgtctg tcttgatgac tggttgattg gctttaaaag cttatgctgt
     5401 actttggccg taatagctct gctaataata taataagcag actcattgtg tttagggaca
     5461 ttgtactgga agaaaacatt ttaaacatca ggcaaataac caagtcacca gctaaataat
     5521 aagttaacag acatgagtcc cgggatgaga ttcaacatta ccattgcccc atttaaagca
     5581 caaaacccgc tcatcagcgg gttttctact ttttcttaac gtcgggtata caaagcccat
     5641 cgttgaaaaa attttatcca tattttttga aaaatgcaaa catcatgtcg ccatcttcag
     5701 caaaaatcat ttatctcgtc accttcctca attgcgcttc cgcgtatgct tcttcctgcc
     5761 agcactttgt taccagttta ccaatgacgt ccgcataccc cttataccac tgataatcgg
     5821 tcaggtctgg taccagcttc tggacatgac gtcgtgccag cgtggtcggt aaacgactaa
     5881 accggtttcc attacaacgc ccacaaatct tatataccgg tacgccatga aaccgggttc
     5941 ttttttcatc cagaacaatc cctttaccct tacaccctct gcacgctgtg ctggcttcgc
     6001 ccttaccatg gcaatgctga catagttcct tcacccattc ttccttgatt acagattccc
     6061 cgcgtctgta gtgtttcacc acttcgcgca atacattata aaatcccgta cctgaacaat
     6121 gctcacagcg agccttactt gccgcagacc tggagtaatc agcaaaggca aaactcacga
     6181 ggtaaggaat aatctgtaac cggatttctt cactcaattt gttcaatgtc gggttatcca
     6241 gtgccatcgc gtaatttagc aggccttcaa tcgcaaactg agggtcctga acaccaactt
     6301 ttgccagaaa taaggccaac ccaagtggtg ctttcgactg caccatcccc tgcgctgcca
     6361 ttacatccgt aattgttaaa caaccggtgc ctgtcgctgg agcgtcatcg ctcaattttg
     6421 gagattttgg ggagtaatat tttggtaagg cttcaaggtt catgctcgtt ctccacttac
     6481 gccagtacgc caattgtcag cgcgcgatcg ataaaacgaa atatcagctc cagttgggag
     6541 ccatacttat cttcaaatgc cactgtatcc gtatgcagct cgttgtgatg ctttctgcac
     6601 aaaggcaaca caaagagatc atgtgctttt gttcccattc cgccctgccc gtgaccaatc
     6661 agatgatgcg gatcgtcggc tggcataccg cagcaagcac acggctgtgt cttaacccaa
     6721 cgtgtgtatt tctccttaac ccagcggcga cgtttaggca gcttcatgaa agattccgga
     6781 gactctggat caacggtgat gcttaccacc gtcttttcct gtggtgattt ttgttgctgg
     6841 tgggcgtaag gcaacggtgc aagatttttt gtgcgttgtt tcaatatgct ggtggcggtc
     6901 tgctctcccg gtacgatgtc gctttcgcgg tacaccgagc agattttttc cgctggtaat
     6961 cccagcgaac gacgcgatac agcctcaggt agtgcatcca ccacctgatt gcagaccgcc
     7021 caccaggata attcagccaa agataattcc cgctcctgcg taccgcttat tgcgtgacgg
     7081 atgacgtcaa tcacccatgc tgtcagattt tgttgagcaa gcagctccag tgattccgat
     7141 gtctggtcac gcagttggtt gtcgcagtgc cagcacaaca ccattgcgcc ggtaccataa
     7201 cggtgaatga ctgtttcagt gtgatggtaa tcgccattag gccactggca ggatgtaaca
     7261 tgacgtaata gccagtcgga caatgcgcca acgccgccag cagcacgaat cacccgttcg
     7321 ttactaaaaa acggcagcaa tgttttgtct tccgccagcg gctggcgaac ggcaggaacg
     7381 actccggatg gcagattacg catgcttttt ggttccggtt ccaccagcac tcgaggatta
     7441 tgaaatatct gtatggattc acggcccggc ttaaggacca ccagcccaag ctcaggcacc
     7501 agaacaggtc taagtaatac ccgcacgtta cctccagatc cgttgctgga aagtgcggga
     7561 cgcacgtggt gggcgtccgg aataaggcag cctgacagag attatccagt gccgatagtc
     7621 gagactgaga gctttcttaa cctcgaaccc gcgcctgcgg taagaatgaa tcagccattc
     7681 ggcctgttct gcagtgcatg gagggtgctg gaaccattca gacttgaatg cgtgagaata
     7741 ccgcccgtgc gtgcaggcaa gaacgggcga attatcagaa ttgtaatatt ttgcgttgcg
     7801 tgccatcggt tttctccggt ggcacggtgt tactcagcgg gagttcagcc ccgcgcaaga
     7861 ttgtagatga gtttattctc ctgaaaaagc agaaaagcca gcttttattc cgatctcttt
     7921 caatacctgt aatgaagtga caaactcacc ttcgcgcaag ataaatccgt ccgtgacccg
     7981 agcatccaca aaattaatta acgcagcccc attctttcgc aaacacacaa tgcggtaatg
     8041 actaacaaga tttccatttt caacgcacac agcatagagg ccatcttcac aaaaaatttt
     8101 acgcagttct tcgatgttca tcatcagaat ccttccggat aattagctct cccctttaag
     8161 ggaccatccc tcttatccct gcgcgctact taagtatttt tgattctatt ccggcaccgt
     8221 ccagaacttc aaatgcgttg aaaataaaaa caaaaacccg ccgaagcggg ttaagtgcgg
     8281 gtgcgttgag aatgcctgcc acatcagagg tggcgaggga tttctccctc gccgggtctc
     8341 ttactcctca ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggattcg
     8401 tacctcgcag aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac
     8461 gatcagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct tgacctttcg
     8521 gtccgtaaga ggctaatctc tatgtgtcgc atagatatgg cctcagatta atgttaagcg
     8581 tcttgcagga cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc
     8641 ctgagacaga tagcctcaag cacccgcagt tattctactt aactaagatt tccccgcaaa
     8701 ccgtttttgt ccggcacagt aaatatccaa ctaaaccaat ggagttcgct gtatttaccg
     8761 ccagtattca atgcacatga ccgccatgaa cacccctaaa aaaagggcat ttatatgtcc
     8821 aaacattaat atcaaaacat caactttttc catatacctt gctgtgaaga tgatgggcat
     8881 acatgatgcg aacaaccaga acgcaacaaa caaaaactgc aatgcgtttt tcattattcc
     8941 tcctacaatc aatgtgcaat tacatttaaa cacacctcaa tttggccgga catataaata
     9001 tctaaaccag aaaaaatcac ttacatagcg ttacaaactc tttagtctaa aggttcatcg
     9061 taaaacattc cccatactta tcagtccgtt ccgcgccagg tagctcattg ccttatctgg
     9121 caacctgtaa tcaggtttcc gctttttcag ttggctggtc gtttaaccga catagttaat
     9181 ccattaatct agttgccgga tgttggtgga ttttcgcgtt ttagttgttc ataaaagtgc
     9241 acagctttaa ccagttcttc tgatgtaact gggactggtg aggcagtgaa taaggcctga
     9301 atttgatagt tcggcctgtc gttacaatcc tcttttttcg gtacatattt ccagtcacca
     9361 gaccactact tcccctgaaa gtccgtaacg ccttttttca cgtagcgata tcgccatgcc
     9421 actggttttg cttgccccgc cgtttcatgc ccttcctgat aattaatctc gctcattcat
     9481 cgccccactc atcacaatat gcttcgaccg gagtttttcc
//
