LOCUS       NZ_CP069919            11338 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed8.
ACCESSION   NZ_CP069919
VERSION     NZ_CP069919.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..135)
                     /locus_tag="I6K64_RS28535"
                     /old_locus_tag="I6K64_28530"
                     /pseudo=""
     CDS             complement(<1..135)
                     /locus_tag="I6K64_RS28535"
                     /old_locus_tag="I6K64_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462379.1"
                     /note="member of the FNT family of formate and nitrite
                     transporters; incomplete; too short partial abutting
                     assembly gap; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /name="nitrite transporter NirC"
     gene            complement(261..587)
                     /gene="nirD"
                     /locus_tag="I6K64_RS28540"
                     /old_locus_tag="I6K64_28535"
     CDS             complement(261..587)
                     /gene="nirD"
                     /locus_tag="I6K64_RS28540"
                     /old_locus_tag="I6K64_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="WP_000084764.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNI
                     DPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDGVV
                     QLRG"
                     /name="nirD"
     gene            complement(584..3127)
                     /gene="nirB"
                     /locus_tag="I6K64_RS28545"
                     /old_locus_tag="I6K64_28540"
     CDS             complement(584..3127)
                     /gene="nirB"
                     /locus_tag="I6K64_RS28545"
                     /old_locus_tag="I6K64_28540"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="WP_000049208.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAYD
                     RVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFYDK
                     LIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEAAGA
                     LKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGVEARK
                     TMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDIYAIGE
                     CASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIGDAHGRT
                     PGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIELPENPDS
                     LILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALKAETKAGTG
                     CGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTFEELLAKHGK
                     GYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYSVIPRSPGGEI
                     TPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAGFETGHAYAKAL
                     RMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCTRECSEAQGKDVG
                     IIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFYIRTADKLTRTAPW
                     LENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVLCEWTETVNTPSAQTRFKHFIN
                     SDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
                     /name="nirB"
     gene            complement(3389..4570)
                     /gene="tsgA"
                     /locus_tag="I6K64_RS28550"
                     /old_locus_tag="I6K64_28545"
     CDS             complement(3389..4570)
                     /gene="tsgA"
                     /locus_tag="I6K64_RS28550"
                     /old_locus_tag="I6K64_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="WP_000185247.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSSM
                     SNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAAMF
                     ILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSIEWY
                     WVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCYILGQ
                     LGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTVLAGLA
                     AILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVLTCGTIG
                     TMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTSH"
                     /name="tsgA"
     gene            4841..5413
                     /gene="ppiA"
                     /locus_tag="I6K64_RS28555"
                     /old_locus_tag="I6K64_28550"
                     /gene_synonym="rotA"
     CDS             4841..5413
                     /gene="ppiA"
                     /locus_tag="I6K64_RS28555"
                     /old_locus_tag="I6K64_28550"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="WP_000477225.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELELD
                     KQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEADNG
                     LRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKIS
                     QVPTHDVGPYQNVPSKPVVILSAKVLP"
                     /name="ppiA"
     gene            5518..5685
                     /locus_tag="I6K64_RS28560"
                     /old_locus_tag="I6K64_28555"
     CDS             5518..5685
                     /locus_tag="I6K64_RS28560"
                     /old_locus_tag="I6K64_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="WP_000736862.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALAR
                     LEELRSHYER"
                     /name="YhfG family protein"
     gene            5675..6277
                     /locus_tag="I6K64_RS28565"
                     /old_locus_tag="I6K64_28560"
     CDS             5675..6277
                     /locus_tag="I6K64_RS28565"
                     /old_locus_tag="I6K64_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="WP_001280641.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRAA
                     TIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNALMQ
                     DLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQLSWQ
                     GIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
                     /name="putative adenosine monophosphate-protein transferase
                     Fic"
     gene            6309..6872
                     /gene="pabA"
                     /locus_tag="I6K64_RS28570"
                     /old_locus_tag="I6K64_28565"
     CDS             6309..6872
                     /gene="pabA"
                     /locus_tag="I6K64_RS28570"
                     /old_locus_tag="I6K64_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="WP_000601847.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKPQ
                     KIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHGKT
                     SPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQWDLE
                     GVQFHPESILSEQGHQLLANFLHR"
                     /name="pabA"
     gene            6958..8178
                     /gene="argD"
                     /locus_tag="I6K64_RS28575"
                     /old_locus_tag="I6K64_28570"
                     /gene_synonym="dapC"
     CDS             6958..8178
                     /gene="argD"
                     /locus_tag="I6K64_RS28575"
                     /old_locus_tag="I6K64_28570"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="WP_000963792.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEYV
                     DFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERVVF
                     MNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDGFGP
                     KPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQHQAL
                     LVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAFHPGSH
                     GSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSDIRGMGL
                     LIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEGMQRFAHA
                     VAKVVGA"
                     /name="argD"
     gene            complement(8245..10335)
                     /locus_tag="I6K64_RS28580"
                     /old_locus_tag="I6K64_28575"
     CDS             complement(8245..10335)
                     /locus_tag="I6K64_RS28580"
                     /old_locus_tag="I6K64_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="WP_001295162.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVPA
                     CCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTAEL
                     GPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQPLRE
                     SLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLSAQNN
                     TDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARLRVLAD
                     DILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPLYARDLL
                     ADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWILMTVLLV
                     TQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASYLILRKNYG
                     WATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQWQSGLLRKNA
                     HDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEPAFNSHYLADM
                     KLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQRLEYDEPGSSG
                     DANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPHHGIWLSRKLRDS
                     KA"
                     /name="YccS/YhfK family putative transporter"
     gene            complement(10386..11018)
                     /gene="crp"
                     /locus_tag="I6K64_RS28585"
                     /old_locus_tag="I6K64_28580"
     ARG             complement(10387..11018)
                     /name="CRP"
                     /gene="CRP"
                     /gene_name="resistance-nodulation-cell division (RND)
                     antibiotic efflux pump"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; fluoroquinolone
                     antibiotic; penam"
                     /identity="99.52"
                     /coverage="100.0"
ORIGIN
        1 aatcaggatg atcccaagac ccacatacgc gcccgccatg gcggagctga cccaaaagcc
       61 gagcgggtta tttgccgaca ggcgtgcaat gcgcgcagcg ttagccgcag acttattaat
      121 agtgtctgta aacatttgat tatcctttaa aaaataaaaa caaaaaataa aacaaaaagt
      181 ttaaaaaatg tcctgcaata taccgaagat tattacagga ccatgcaaaa aggggaggca
      241 ttgcgcctcc cgttaaaaca ttaaccgcgc agctgcacca cgccgtcttt cactcgcgct
      301 tcgtaatgtt tgacggaaaa ctgttcgtct tccatgcaca agccgtcgct taagcgaaaa
      361 cgctgttttt tcagcgggct ggcgacccac agctcgccct ggtgttccgc aatcagtccg
      421 cgtgacagca cgctggactc gaagaacggg tcgatgttgc tgatcgcaaa cacctgatcg
      481 ctgtgatacg ggcggaaaat cgcgacctgc tcgtcaccta acagcgcgca gacgccggtt
      541 tcaggcagga tgtcatcgat tttgcagatg tctttccact ggctcatgcg ttgtcctcca
      601 ccagagttac tgggatacgt tcatacggcg ttgccggacg gtgctgttcg cgctctggca
      661 ccatctgcac gttcgggtca cgcttgtcgc tgttgatgaa gtgtttgaag cgagtctgcg
      721 cagacggcgt attgaccgtt tcagtccact cacacagtac cgcttcacgc aggcgcgcca
      781 tctcttcttc cagatgtgcg ttcagcccca gtttgtcgtc aatgatcact gctttcaggt
      841 aatcgatgcc gccttcgagg ttttctaacc acggtgcggt acgcgtcagt ttgtcggcag
      901 tacggatgta gaacatcatg aagcggtcga gatatttgat cagcgtttcg cgatcgatat
      961 ccgccgccag cagatccgca tgacgcggtt tcatgccgcc gttaccgcaa acatacaggt
     1021 tccagccttt ttcagtggcg ataataccca cgtctttacc ctgagcttct gaacattcac
     1081 gggtacagcc ggagacaccg aacttcattt tgtgcggcgt acggatgcct ttgtagcggt
     1141 tttccagttc cacgccgagg ccgacgctgt cgccaacgcc gtagcggcac caggtgctac
     1201 ccacgcaggt tttcgccata cgcagtgctt tcgcataggc atgaccggtt tcgaagccgg
     1261 cttcaatcag ctgacgccag atctccggca gatcgtcttt ctgtgcgcca aacatcgcca
     1321 gacgctggga gccagtgatc ttggtgtaga gattaaattc acgcgcgata cgacctaccg
     1381 ccatcagccc ttccggggtg atttcaccgc ccggagaacg cgggatcacc gagtaggtgc
     1441 cgtctttctg gatgttagcg aggaagttgt cgttagaatc ctgcagcgga gtatgttccg
     1501 gcttcagaat gtattcgttc cagcaggagg ccagcagcga accgacggtt ggtttacaaa
     1561 cttcacaacc gtagcctttg ccgtgtttcg ccagcagttc ttcgaaggtt ttaatgcctt
     1621 caacgcggat caaatggaac agttcctgac gcgaataagc aaagtgttcg cacaggttgt
     1681 tgttaacttc gatgccctgt ttcgccagtt ccgcgttcag tacctgagtg accagcggga
     1741 tacagccacc gcagccagta cccgctttgg tttcagcttt cagcgccgca actgtgtggc
     1801 agcctttgtt gatggcagca atcagatcac ctttggtgac gtcgaagcag gagcagattt
     1861 gcgcgctgtc cggcagttta tcaacaccga tagacggctt gccgctaccc gagtgtgctg
     1921 gcaggatcag ggaatccggg ttttccggca gttcgatagc gttcagcacc agttgcagca
     1981 ggttaccgta gtcgctggta tcgcccacca gtaccgcacc gagcagggtt ttgttgtctt
     2041 cgctgacaat caggcgtttg tagatctctt tactttcgtc gaggtaaacg tagctacgtg
     2101 cgccaggcgt gcgaccgtgc gcatcaccaa taccgcctac gtctacgccc agcagtttca
     2161 gcttggcgct aaggtcagca ccttcaaagg cgttttcgct accgagaata tggtcaacgg
     2221 cgacctgcgc cattttgtag ccaggtgcta ccagaccaaa tacacggttg ttccagcttg
     2281 cgcattcacc gatggcgtag atatccggat cggaagtctg gcaggaatca ttaatgacaa
     2341 tacccccacg cggagcaacg tccagaccac actgggttgc cagcttatcg cgcggacgga
     2401 taccggtaga gaagacgata aagtcgactt ccagttcgct gccgtcggca aaacgcatgg
     2461 ttttacgcgc ttcaacacct tcctgcacaa tctcaagggt gtttttgctg gtgtgaacgc
     2521 gcacgcccat actttcgatt ttgcgacgca gctgctcgcc gcccatctga tcaagctgtt
     2581 ctgccatcag cataggggca aattcgataa cgtgggtttc aatacctaag tttttcagcg
     2641 cgcctgcggc ttccagacct aacaggccgc caccaacaac ggcaccgcgt ttgctgcgac
     2701 gggcgcagga ttcaatggcg ttgaggtctt caatagtgcg atagacaaag cagtcctgag
     2761 tatcagaacc tttgattggc gggatccacg ggtaggaacc ggttgccatg atcagcttgt
     2821 cataaaaaac ggtacgtccg gcgctggagt gaatcacctt ctcctgacgg ttgatggtga
     2881 tagcgcgttc gccgaccaga actttgatgc cgtgtttctc gtagaagcct tcgcgcacca
     2941 gcgacagctc ttcggcggtg tggtgagaga agtaagacga gaggtgtacg cggtcataag
     3001 cgatgcgcgg ttcttcacag aaaacggtaa tatcaaagtt ggccgcatca gatttatcaa
     3061 gaagatcttc gataaagcga tggccgacca taccgttacc gataattgcg agtctgactt
     3121 tgctcatttt tgcctcgatt tcttttctat taccgcctac cttaacgatt cagcaacccc
     3181 gcttattgat gtaaatcaaa ttcaccttta tatactcctt aatgggtata ttgctgattt
     3241 gtataaattt tcttaagtca cggaattgtc tgatatttca tgtttgtgca aaaaatagac
     3301 aaaatgagcg ggtttataga aatagatgca cgtagcgggg gaagagagga aaaggccgga
     3361 cgacgatagt gccgtccggc atgagcaatt aatgagaggt cagggtgtta tgctgacggt
     3421 gacggctgac gaaacctaac aggaagcaca tcacaaagac gacagcgtac agaccgtttg
     3481 ctgtaagcag tgccgcctgc ggaccgctat gttcaacgat cgggccggta accacaaagg
     3541 tcaacatagt accgatggtt ccgcaggtca ggacaaagtt aaccagtttt ggcgacggta
     3601 ctttggtctg ctgtgaaccc agggtgatga tggtggtata gatcgcgctg gagaagaagc
     3661 ccagagcgag aattgaccac gccatatgtg ctggtgttcc ggtgttaaag acgtacatca
     3721 gaatcgcagc cagaccagcc agtacggtca gaatgcgttg caaatcaaag aagcgaagaa
     3781 taaagctgaa cgcccacatg ccgaccatgt atgacatcca gaagttactc accagcgtgc
     3841 ccgcgtcgtt caggctcatg cccaggcctt tggcatactc aggcacccag gagataaaac
     3901 ctaactgacc gaggatgtag cacaacgccg caacagagag aaacagtacg ccgatccccc
     3961 acttttcttt ctctaccgga gcatccgttt ttggcgcatg tttgcccagc gccgggaact
     4021 cacagccgaa ggtcagaata aaaatagcga catacaccag cccgatgcag gcataaaccc
     4081 agtaccactc aatgctgcgc gccagtagaa acgcggcgat cattgggaaa atcatcccag
     4141 ccatactgaa gaaggagtcg gtaaataaca ggcgggaacc gcgctgacgc ccttcataca
     4201 tttgtgttac caggaatgta ccaatcgaca tggtgatgcc gctgaccacc ccgagaatga
     4261 acatcgccgc cgagaacagc gccaggctgt ggctgaacat caaaccggca accgccagca
     4321 ccatcaggag aaagccaaaa cgtaactgcg ttttcaacgg gacgatttcc atcagccagg
     4381 cgttgaggaa gatagagatt aaaatgccgg cgttgaggaa ggtgaaggta ttactcatac
     4441 tggaaacagg cagattgaaa taatcggcga tatttcccat caccatcccg gtgacaataa
     4501 ccaacgcacc agtcagtgcg taggagagaa agctaatcca tgtgagcttg atgcgattgc
     4561 tgttagtcat gattggcctg cgttcaaaaa taaaatggca tagcgggata tgccgcgagc
     4621 gggcgatttt aggtgatttt gtgatctgtt taaatgtttt attgcaatcg gttgctaaat
     4681 tgcattttaa gaggtgattt tgatcacgga ataaaaagtg atcgtcaggt tacatatatt
     4741 tcagatacgt aaaattaggt aaagggatgg ccttgttctt gaaggctatt tagaatctct
     4801 tcacttgctt tttttctgct ctgtttgtta aggaaatctc atgttcaaat cgaccctggc
     4861 ggcgatggct gctgttttcg ctctttctgc tctttctccc gcagcaatgg cagcgaaagg
     4921 ggacccgcac gtattgttga caacctcagc tggtaacatc gaactggagc tggataaaca
     4981 aaaagcgcca gtgtctgtgc aaaactttgt cgattatgtg aacagcggtt tttataacaa
     5041 cactaccttt caccgcgtca ttcctggctt tatgattcag ggcggcggtt tcaccgagca
     5101 gatgcagcag aaaaaaccaa acccgccaat caaaaatgaa gccgataacg gcctgcgcaa
     5161 cacgcgtggc accatcgcga tggcacgtac cgctgacaaa gacagcgcca ccagccagtt
     5221 ctttatcaac gttgccgata acgccttcct tgaccatggt cagcgtgatt tcggttacgc
     5281 ggtatttggt aaagtggtga aaggcatgga cgttgccgat aagatttccc aggtgccgac
     5341 tcatgacgtt ggtccgtacc agaatgtgcc gtcaaaaccg gtagttatcc tttccgctaa
     5401 agtcctgccg taatgatttc tcgcgcgggc aatcttgccc gcgcttctgc tctcccggcg
     5461 taacccggat ttgccgctta tacttgtggc aaatggacac gttcagggag gcatcaagtg
     5521 aagaaactca ccgataagca aaagtcccgt ctctgggagc ttcagcgtaa tcgtaatttt
     5581 caggccagtc gccgtcttga aggcgtcgag atgcctttag tcactcttac tgccgcagag
     5641 gctttagcgc gccttgaaga gctgaggagt cactatgagc gataaattcg gcgaagggcg
     5701 cgatccgtat ctttatccag gccttgatat catgcgtaac cggctgaaca tccgccagca
     5761 gcagcggctg gaacaggccg cttacgaaat gacggcgctg cgtgctgcga ccattgagct
     5821 tggtccgctg gtgcgcggtt taccgcattt gcgaactatc catcgccagc tgtatcagga
     5881 tattttcgac tgggcagggc aactgcgtga agttgatatt tatcagggtg atacgccgtt
     5941 ctgccacttt gcttatatcg aaaaagaggg caatgccctg atgcaggatc tggaggaaga
     6001 aggttatctg gttggcctgg agaaagcgaa gttcgtcgag cggctggcgc attactattg
     6061 tgaaatcaac gtgctgcatc ccttccgggt gggaagtggt ctggcacagc ggatcttctt
     6121 cgagcaactg gcgattcatg ccggatatca actgagctgg cagggtatcg aaaaagaggc
     6181 ctggaatcag gcaaatcaga gtggggcaat gggggatctc accgcactgc agatgatatt
     6241 tagcaaagtg gtaagcgaag ccggggaatc tgagtaaaat agcgcggttc ttttgtaccg
     6301 gagccgccat gatcctgctt atagataact acgattcttt tacctggaac ctctaccagt
     6361 acttttgtga actgggggcg gatgtgctgg ttaagcgcaa cgatgcgttg acgctggcgg
     6421 atatcgacgc ccttaaacca caaaaaattg tcatctcacc tggcccctgt acgccagatg
     6481 aagccgggat ctcccttgac gttattcgcc actatgccgg gcgcttgccg attcttggcg
     6541 tctgcctcgg tcatcaggca atggcgcagg catttggcgg taaagttgtg cgcgccgcaa
     6601 aggtcatgca cggcaaaacc tcgccgatta cacataacgg tgagggcgta tttcgggggc
     6661 tggcaaatcc acttaccgtg acacgctacc attcgctggt ggtggaacct gactcattac
     6721 cagcgtgctt tgacgtgacg gcctggagcg aaacccgcga gattatgggg attcgccatc
     6781 gccagtggga tctggaaggt gtgcagttcc atccagaaag tattcttagc gaacaaggac
     6841 atcaactgct ggctaatttc ctgcatcgct gatttctgat tgccatttag tgatttttta
     6901 tgcatatttt gtggttataa tttcacattt gtttatgcgt aacagggtga tcatgagatg
     6961 gcaattgaac aaacagcaat tacacgcgcg actttcgatg aagtgatcct gccgatttat
     7021 gctccggcag agtttattcc ggtaaaaggt cagggcagcc gaatctggga tcagcaaggc
     7081 aaggagtatg tcgatttcgc gggtggcatt gcagttacgg cgttgggcca ttgccatcct
     7141 gcgctggtga acgcgttaaa aacccagggc gaaactctgt ggcatatcag taacgttttc
     7201 accaatgaac cggcgctgcg tcttgggcgt aaactgattg aggcaacgtt tgccgaacgc
     7261 gtggtgttta tgaactccgg cacggaagct aacgaaaccg cctttaaact ggcacgccat
     7321 tacgcctgtg tgcgtcatag cccgttcaaa accaaaatta ttgccttcca taacgctttt
     7381 catggtcgct cgctgtttac cgtttcggtg ggtgggcagc caaaatattc cgacggcttt
     7441 gggccgaaac cggcagacat catccacgtt ccctttaacg atctccatgc agtgaaagcg
     7501 gtgatggatg atcacacctg tgcggtggtg gttgagccga tccagggcga gggcggtgtg
     7561 acggcagcga cgccagagtt tttgcagggc ttgcgcgagc tgtgcgatca acatcaggca
     7621 ttattggtgt ttgatgaagt gcagtgcggg atggggcgga ccggcgattt gtttgcttac
     7681 atgcactacg gcgttacgcc ggatattctg acctctgcga aagcgttagg cggcggcttc
     7741 ccgattagcg ccatgctgac cacggcggaa attgcttctg cgtttcatcc tggttctcac
     7801 ggttccacct acggcggtaa tcctctggcc tgtgcagtag cgggggcggc gtttgatatc
     7861 atcaataccc ctgaagtgct ggaaggcatt caggcgaaac gccagcgttt tgttgaccat
     7921 ctgcagaaga tcgatcagca gtacgatgta tttagcgata ttcgcggtat ggggctgttg
     7981 attggcgcag agctgaaacc acagtacaaa ggtcgggcgc gtgatttcct gtatgcgggc
     8041 gcagaggctg gcgtaatggt gctgaatgcc ggaccggatg tgatgcgttt tgcaccgtcg
     8101 ctggtggtgg aagatgcgga tatcgatgaa gggatgcaac gtttcgccca cgcggtggcg
     8161 aaggtggttg gggcgtaaat acgaagcatc aggcaaaaga tgtcggatgc ggcgtgaatg
     8221 ccttatccga tggtttagcc tgcattacgc cttcgaatcc cgcaacttgc gactcagcca
     8281 aatcccgtga tgcggtcgct gacgccatgc catcgacgaa atggtgtgca tggtgttcag
     8341 atgaccaata acccgctgta aatgctgctc cagcgtacct gccgcgcctt cgtgcggctg
     8401 catctccggc gcatccatga tattggcatc gccagaacta cccggttcgt catactccag
     8461 tcgctgctga caacgctgaa tggcgatttc acaagactgt aaatactctt gtgccagttc
     8521 aggtggcaat gcccggtgtt cccgcgccag cgtggtcatg gcattaatat gctcaacaat
     8581 aaactggctg tgcgttaccc acagtttcat atctgccaga taatggctgt taaacgccgg
     8641 ttcctgcatc gcctgattca atgagttata cagagtgtta tgtgcctgat ttacccgcat
     8701 tcgctgccag gccagtggcg taggttgcgg atcctcgcta agaatcaagc gaatcgcttc
     8761 ctgataggct tctaaagcat catgggcgtt tttacgcaat aacccgctct gccactgcgg
     8821 ccacagccag acagtaccgc cgaaagcaat taaacaacca ataatggtat cgataagacg
     8881 cggaaggatg tattgctcgc cgttcaacca caatagttgc agggtataca ctgcggtaat
     8941 agtaaaaccg accgtcgccc agccgtagtt tttgcgcaat atcaggtagc tggcgagggt
     9001 ggtaatcagc atcaacgtca gggtgtaacc ttcgggaatt ttaaagtgca gcgccacgcc
     9061 cgcaatgatt aacccgacaa cggttcccac ggagcgattc acaatcctca gacgggttgc
     9121 gccatagcca ttttgtgtca ccagcaatac cgtcatcagg atccagtacg acttcggcag
     9181 atgcagcgcg gtgcccatca ggctggcaac gcttaacatc acactgagtc gtccggcatt
     9241 gcgtagcgcc ggagacttta gtgacagata acttttcagc gccggaagta atggcattcg
     9301 ccgctgttta tcggccagta agtcacgggc atagagcggt ttttgggtgc gcagcacgcg
     9361 ggcgatgcgg ctgaaatgcc agtagcagaa ttgcccaacc ggattatccg gatgctggcg
     9421 ggcgattttt tccagtgcgc caatttgctt ttccatcgta aaacgcgttg gcaggcgatg
     9481 gtaaagaatg tcatcagcca gcacgcgcag gcgagcggcg acggtttgcg cattccagcg
     9541 gataacttct tccgcatggc tacgctcgac cagcttttgc acctcttccg gctgatgcaa
     9601 actgaccgaa atatgttcct gtaaatccag cgcctcctgg aaaatacgca gcatccgctt
     9661 gtagtcagta ttattttgcg cggaaagcat atgcatttgc tgatagcact gggtaattag
     9721 atcgaccgct ttttgctggc gcaccagcag cggcggcagc gctttttcag ggtcggtgtg
     9781 ctgggtaagc aggctgtatt tggcttcaca ataatctgcc agttcacggt acagcagact
     9841 tagtgactcg cgcagcggtt gttcgcgcca gatccagaac caaaaccagt taaacaatcc
     9901 gtaccagaga gtgcccaacg catagatgag caacggttcc cagaccggca tgtatcccgc
     9961 caaactgagg gtaaaaatgg cggcgagcag cgatgcagga agcaattttg cgtgcaatgg
    10021 ccccagctca gcagtgacgc caagtaccag cgttaatccg gtcagcaaaa agggcagggg
    10081 aacatctttt gccagtagta gctgtgtcag caagctacag gtggcaaaca gcgacgcacc
    10141 aatgattaag cgtttgaaaa aacgtttatg aggcgtatca aggcccgcaa tattgcaaca
    10201 ggcaggaacg agggagaaga gcagaccgaa tcgtaattcg ccaagcatta acccaacggc
    10261 cacgggcaaa catagcacca gcgtttgtcg aagtgcatag ttgatatcgg ggtgataaat
    10321 cagtctgcgc cacatcgggg gaaacaaaat ggcgcgctac caggtaacgc gccactccga
    10381 cgggattaac gagtgccgta aacgacgatg gttttaccgt gtgcggagat caggttctga
    10441 tcttccagca tcttcagaat gcgtcccacg gtttcacgag aacagccgac aatctgacca
    10501 atttcctgac gggtaatttt gatttgcata ccgtccgggt gagtcatagc gtctggttgt
    10561 tttgccagat tcagcagagt ctgtgcaatg cggcccgtca cgtcgaggaa cgccaggttg
    10621 cccactttct ctgaagtgac ttgcagacga cgcgccatct gtgcagacaa acgcatcaga
    10681 atgtccgggt ttacctgaat caattggcga aattttttgt acgaaatttc agccacttca
    10741 caggcggttt tcgcacgtac ccatgcgcta cgttcctggc cctcttcaaa caggcccagt
    10801 tcgccaataa aatcaccctg attcagatag gagaggatca tttctttacc ctcttcgtct
    10861 ttgatcagca ctgccacaga gcctttaacg atgtagtaca gcgtttccgc tttttcaccc
    10921 tggtgaataa gcgtgctctt ggatgggtac ttatgaatgt ggcaatgaga caagaaccat
    10981 tcgagagtcg ggtctgtttg cggtttgcca agcaccatgc gcggttatcc tctgttataa
    11041 gctttctcca gagccagata acgccgctgt ctctggattg ccgaaatatg cttcccgcta
    11101 cctgggaagg ggctatcaac tgtactgcac ggtaatgtga cgtcctttgc atacatgcag
    11161 tacatcaatg tattactgta gcatcctgac tgttttagca tagctttcgc tttgtgtctc
    11221 ctggtgtctc gcttcagcat gacccaggtc gccttccgtt gcgcgatttg gttagtacgc
    11281 gtactctgtc aggaaaattg acgcagtgga gtagcaaaaa tgcaagcgcg agtgaagg
//
