LOCUS       NZ_CP069913             4420 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella oxytoca strain FDAARGOS_1334 plasmid unnamed19, complete
            sequence.
ACCESSION   NZ_CP069913
VERSION     NZ_CP069913.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357476
            Assembly: GCF_016903415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 591.2x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:56:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,798
            Genes (coding)                    :: 5,689
            CDSs (with protein)               :: 5,689
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 70 of 109
            Pseudo Genes (internal stop)      :: 26 of 109
            Pseudo Genes (multiple problems)  :: 29 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069913.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1334"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1334"
                     /db_xref="taxon:571"
                     /plasmid="unnamed19"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(759..1169)
                     /locus_tag="I6K64_RS28120"
                     /old_locus_tag="I6K64_28115"
     CDS             complement(759..1169)
                     /locus_tag="I6K64_RS28120"
                     /old_locus_tag="I6K64_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714791.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKSIKVID
                     TSELIRVFGSLDGVIDDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1169..1360)
                     /locus_tag="I6K64_RS28125"
                     /old_locus_tag="I6K64_28120"
     CDS             complement(1169..1360)
                     /locus_tag="I6K64_RS28125"
                     /old_locus_tag="I6K64_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_025714792.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELLDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1832..2023)
                     /locus_tag="I6K64_RS28130"
                     /old_locus_tag="I6K64_28125"
     CDS             complement(1832..2023)
                     /locus_tag="I6K64_RS28130"
                     /old_locus_tag="I6K64_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2443..2574
                     /locus_tag="I6K64_RS29645"
     CDS             2443..2574
                     /locus_tag="I6K64_RS29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741455.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGKQDVREM"
                     /name="hypothetical protein"
     gene            2899..3822
                     /locus_tag="I6K64_RS28135"
                     /old_locus_tag="I6K64_28130"
     CDS             2899..3822
                     /locus_tag="I6K64_RS28135"
                     /old_locus_tag="I6K64_28130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204724554.1"
                     /translation="MTAQDLINVLTILKANDSTSFSKIQRALKMSISQLEGIIDGLTAM
                     GIVYKSSFTSYSLTELTSKPVVSDGVRKAFEDIITNRGTYLSEELLQKVSTPFIPLMTH
                     EYKNAPVKVMIVGQETLGMEDAFSTIVSVDDYINESIESFNKFNFGEDLRNSHFWYAFD
                     EVVKYFNLPSRRHAYWTNLHKFQLIENDGDSVSISKLPSKDIMTMIHMQRELFLAEIKD
                     TRPDIIIYFTGGQTWVLDHYLNNGKKLAVKAIDERSHLGIIQTEFLHCPIAVCTDHPAR
                     RGYTQAILDHRSNLLKYAADKFLASR"
                     /name="hypothetical protein"
     gene            complement(3937..4308)
                     /locus_tag="I6K64_RS28140"
                     /old_locus_tag="I6K64_28135"
     CDS             complement(3937..4308)
                     /locus_tag="I6K64_RS28140"
                     /old_locus_tag="I6K64_28135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065809893.1"
                     /translation="MSKKNDLNLSESTLESLREYVDDFGMTVDDVVLKLISENKSLKES
                     LGQDFVLSGEKEAHHFKDYVIHKLPKGKILVTRNGLKASNMKKILIEAGRELGMDENEL
                     NPDFTTYEIGRFLFKHLHA"
                     /name="hypothetical protein"
     Typing          1899..2519
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.639"
                     /coverage="99.0"
                     /evalue="5.52e-168"
ORIGIN
        1 aaagaacggc tacaccgctg gatttaccat aacatccgtt atcagcaccg cctcaggcgc
       61 cctcagcgcg tttctgacgg gaaaccggca aaacaggcag caaaccaccc gaaaacccgc
      121 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcgcaag tgcatagcgc
      181 ggaaattcac gccattcagc cggggtaagt cctgtttccg atgttcttct tacccgaaaa
      241 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
      301 ccggttttca gcacgttcag gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa
      361 gacgccggac gactcaccac acgattcacc ggcgattcga ctcgtgactt gactctagag
      421 tcaccacggt caaaaaaacg ccaaagcgac gattcgactc tagagtcaag tcgtcataag
      481 ttactgatag tgtggagtga gtcgtcgatg actctatgac gagtcgtcgg taagtcgtac
      541 acgaatcacc gacgacttgg attgacctta cagaaggtaa tactgcgtaa tttaataaaa
      601 cctaaccaac tgataataag gagttatctg caaaatttta attttgcgtg gtgtgtgggt
      661 atttttcgtg gtgaggagtg ttcccggatc tggcgatttt tgggaaggtt caggaacgcc
      721 ccgggatggc cggagaggaa aactcaagca tggcgggatc aggatttttt ccagaatttc
      781 caccacgggg aatggggtgg tgaagaaggt tcgtgcttgt gctccagaag ctgcatagcc
      841 tgacgaaggg aatcaatatg ctcatctcga gccttaacag cccctttaag accatcgttt
      901 tcagcttcaa gtaaagcgat tctatgttgt aaatcatgta aaccaacgct gttaatacct
      961 gtagattctg cgtcactttt tacatcatcg atgacaccat caagagaacc aaacacccta
     1021 ataagctctg atgtatcaat aactttaata ctcttcccgt gctttacaac agaggtgtaa
     1081 cttaacttac cactgttaat catcttgtaa atagtagggc gactaacctt aacaagccgt
     1141 gctgcttcac taatagacac tctggccatc agttaccatc cagtaattca tccagtctcg
     1201 aggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa aggtcggcct
     1261 cagcatcgag atcaagctcg ttaagcttct ccagcaggag cagagactga ttctggatga
     1321 atttagccat gttcagcgct gttttctgtt gtttcgtcat aagggacctc agagcatcag
     1381 tgctcagtgt aaaaccggta agggggtcgg atgtacgaat ggttcagaga acggatctga
     1441 ggtaatcctg tagttatttt gtagtttatc tgtagtcatc gcactaaaaa tcaggattaa
     1501 gtcatcaact tttgtagtct tcctgtagtt tacctgtagt ccggaaaaat aatccgttca
     1561 tgcaggcatg aacgatacag tgaggaacgc tttttttgtc acgatctacc cttaacctgc
     1621 gtaaatcacc cgccatgatt gattattttt caacctcaaa atggtcatac acctaacgca
     1681 gctgcgattt tcatcgcaac ttcatgaaaa ataaataaaa aagaaaaaat acagggattc
     1741 tgggtattac cggggtaata atgttaagcg ctaacacaat gaaaattaag gcgatgccgg
     1801 tattactccg gtaatactta gaacggagac attacgcacc gtcctgctgc tcattcaggc
     1861 gcgctgacag gcttcggtaa aggcgttccg catcctcatg gagtttctcg cagaaatcag
     1921 cctcatgatc cagatccagc tcattaagcc tgtccagaag aagcagggac tgatcctgga
     1981 tgaatttagc catgttaacg gctgtctttt gttgtttgtt catgaaggcc ccggcgcgaa
     2041 agtgcttact gtacgttctg attgttttcc gcttcctcgc tcattcgctc actccgttcg
     2101 gtcatgagtc tgcggcgagc ggagggaaga taagcagata acctaacggt aagcataacg
     2161 aaacctaana acaagggcga aaaggccacg ccccgccggg gcgtttttcc ataggctccg
     2221 ccccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     2281 gactataaag ataccaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg
     2341 cctttcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat tccacgcctg
     2401 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     2461 cagtccgact gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     2521 gcaaaagcgc cactggcagc agccactggt aagcaggatg tgcgagagat gtagtaagag
     2581 ttcttgaagt gatggcctga gtgcggctac actgaaagga caaatttggt gacatgtctc
     2641 gcacaagact gttacctggt taagcagttc cccaactgac ctaaccttcg atcaaaccac
     2701 ctccccaggt ggttttttcg tttcttggag aagagactac gacgacaaat gacggatctc
     2761 aagaagatcc ttttaacgta caaaaatttc cgttctccaa aggtgatgct gccaacttac
     2821 tgatttagtg tactgcgacc gtcagaatga gttaaacgcg tatctacatt gaagcagttc
     2881 aatatgagga aaacaggaat gacagcacaa gatttaatca acgttctcac tattcttaag
     2941 gccaacgact caaccagttt tagcaagatt caacgcgcgt taaagatgag tatttcgcag
     3001 ttggagggca tcattgatgg gttaactgca atgggaatag tgtataaatc atcattcact
     3061 tcatacagcc ttactgagtt aacctctaaa cctgttgtta gtgacggtgt aagaaaggca
     3121 ttcgaagata tcattaccaa tcgcggtact tacctgtcag aagaactact acaaaaggta
     3181 agtacaccat tcatcccact gatgacgcac gaatacaaaa atgccccagt taaagtgatg
     3241 atcgtggggc aagaaactct gggaatggaa gatgctttca gtaccatcgt ttcggtcgat
     3301 gactacatta atgagagcat tgaatccttc aataaattca attttggtga agatttaagg
     3361 aatagccact tttggtatgc atttgatgaa gtagtaaagt attttaacct accatccaga
     3421 aggcatgctt attggactaa tcttcataag tttcaattaa ttgaaaatga tggagatagt
     3481 gtcagcattt ccaaactacc ttcaaaagat atcatgacaa tgattcatat gcagcgtgaa
     3541 ttattcttgg ctgaaattaa agataccagg ccagatatca tcatctactt cacgggtggg
     3601 caaacctggg tgttagatca ttatctaaac aatggtaaaa aattagcagt taaggcaata
     3661 gatgaacgct cgcatctggg gattatccag acagaatttt tgcattgccc aatcgctgta
     3721 tgtaccgacc atcctgccag aagaggatat actcaagcca tcttagatca tagatctaac
     3781 ctactgaaat acgctgctga taaatttctt gcaagtcgat aatttccaat tgattgtgta
     3841 ctaagaccct ccatttttaa cggaaggttg tatctaatcc tggtggcagg tcagggcagc
     3901 gaagttcgat tcctgccctt caggcctgca ggattgttac gcatgtaggt gcttaaacaa
     3961 aaaacgccct atctcatagg ttgtgaaatc tggattgagt tcattttcat ccatgcctag
     4021 ctccctcccc gcctcaatca atatcttctt catgttgctg gccttaagcc cattacgggt
     4081 aaccagaatt tttcctttgg gcagtttatg aatcacataa tctttaaagt ggtgcgcttc
     4141 cttttcgcca gataacacaa agtcttgtcc aagtgattcc ttcaatgact tattctcact
     4201 aattaacttc agaacaacat catcaacagt catgccgaag tcatcgacat actccctgag
     4261 gctttctaac gttgattcac taaggtttaa atcgtttttt ttactcatgt agtcaccaat
     4321 atgctagttt tcagaaaaaa caaaatacaa cacaagtggt atagtacacc actttttagc
     4381 attgaacaac caacatggaa gtcactgatc gctggttcaa
//
