LOCUS       NZ_CP069909             4809 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1313 plasmid unnamed8,
            complete sequence.
ACCESSION   NZ_CP069909
VERSION     NZ_CP069909.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357455
            Assembly: GCF_016903395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1345.58x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:17:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,468
            CDSs (with protein)               :: 5,468
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 77 of 165
            Pseudo Genes (incomplete)         :: 94 of 165
            Pseudo Genes (internal stop)      :: 28 of 165
            Pseudo Genes (multiple problems)  :: 29 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069909.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1313"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1313"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            1168..1359
                     /locus_tag="I6K43_RS28550"
                     /old_locus_tag="I6K43_28555"
     CDS             1168..1359
                     /locus_tag="I6K43_RS28550"
                     /old_locus_tag="I6K43_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1359..1760
                     /locus_tag="I6K43_RS28555"
                     /old_locus_tag="I6K43_28560"
     CDS             1359..1760
                     /locus_tag="I6K43_RS28555"
                     /old_locus_tag="I6K43_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2602..2811)
                     /locus_tag="I6K43_RS28560"
                     /old_locus_tag="I6K43_28565"
     CDS             complement(2602..2811)
                     /locus_tag="I6K43_RS28560"
                     /old_locus_tag="I6K43_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(3515..4060)
                     /locus_tag="I6K43_RS28565"
                     /old_locus_tag="I6K43_28570"
     CDS             complement(3515..4060)
                     /locus_tag="I6K43_RS28565"
                     /old_locus_tag="I6K43_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310050.1"
                     /translation="MASYFPTLDISGEEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEAFYHPAGEQIIYDHSGNGRTFCPIRYRLSLALPEIINRLCDPAEKVYQTSAK
                     RNWAYSITIDDPEGPYHLFFEIRRASRDEKHLQDLNMVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     gene            complement(4064..4633)
                     /locus_tag="I6K43_RS28570"
                     /old_locus_tag="I6K43_28575"
     CDS             complement(4064..4633)
                     /locus_tag="I6K43_RS28570"
                     /old_locus_tag="I6K43_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310049.1"
                     /translation="MAKLPLPLPIASPPTVSNSGSERAKKSGRRTLSAKRKTLLEELWG
                     DELEQLKVWNRHEHDGYTTVPRTLTYISRILDYLGGTGLPLSQTYLALWCRVFDEGFVE
                     IRDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTVIKE
                     LYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     Typing          complement(645..1047)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.35"
                     /coverage="99.0"
                     /evalue="3.56e-168"
ORIGIN
        1 actgatattc aacgagttat gacaggtttc tgtgctcgct gtgcttaatt gtactcaatg
       61 tgctgaatgt gctcgcggtg ctgaatgggg atgcaagggg gcaaggtaat aattttacgg
      121 cagccattta atgcaaatcg atatgcctgc tcatagtgcc ctgtgttcga ttttcggtgg
      181 tttttatggt tatggtgtgg tttggggcag gaaatgactt ggctcttgcc tgagcgcgtc
      241 tgagagctat gtgtggatgt tgatgggggt agcttggaaa acgggaatta tcttacgtta
      301 agcgttttga gcttaacggg taatttcgct ccagtgagcg gaatcacctg accgtagttg
      361 tatatggttt taatcgacgg tgacgggagt tgaatgtgat agtgaagcga gctgcaatta
      421 gttaaggatc ttcttgagat cccatttgta tcgtcgtaat ctcttgctct gaaaacgaaa
      481 aaaccacctg gggaggtggt ttgatcgaag gttaagttag ttggggaact gcttaaccag
      541 gtaactgtct tgtgcgagac gagtcaccaa aactgttctt tcagtgtagc tgtagtccgg
      601 ccatcacttc aagtactctc taaaatctct cgcacattga ctcttaccaa tggctgctgc
      661 cagtggcgct ttgtcgtgtc tttccgggtt ggactcaaga tgatagttac cggttaaggc
      721 gcagcggtcg gactgaacgg ggggttcgtg cagacagtcc agcttggagc gaactgcctt
      781 ctcggaactg agtgtctggc gtggaatgag ataaacgcgg ccataacagc ggaatgacac
      841 cggcaaaccg aatggcagga acaggagagc gcacgaggga gccatcaggg ggaaacgcct
      901 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attctgtgat
      961 gcttgtcagg ggggcggagc ctatggaaaa acggcaacgc cgcggccttt cctttctcca
     1021 tcacgcgatt gctcggtttt ctcgagcttt tgtacgcatg ctcctgccgc tcgccgcagc
     1081 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaagagaa catccctaca
     1141 ctgagcactg acgctgtgag gcctcttatg acgaaacaac agaaaacagc gctgaatatg
     1201 gcaaaattca tccagaatca gtctctgctc ctgctggaga agcttaacga gcttgatctc
     1261 gatgtcgagg ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca
     1321 ctgtcctcga gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg
     1381 aggctgcacg actgacgggt aagagtagaa caacactgca caggttaatt aaagcaggtg
     1441 acctgtccac ctgtagcggt gaacgtaatg caaaaatggt agatacttca gagcttttac
     1501 gtgtctttgg tccctttgag caaccgaagg gtgaacacca ttctggacag gtctctgaac
     1561 agcgtgatac agcctctgct gaccagagtg aacaggtgat tcgtcatctg aaacaggaga
     1621 ttgaacacct gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg
     1681 ccatgctcct tcttgaaaat caccaggcaa aattaactga atccccggcg ccatggtgga
     1741 aattctggaa aaaatcctga ccccgtcatg cttcagtttt cctctccggt cattccgggg
     1801 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac
     1861 ccacacccca cgcaaaatta aaattttgaa gataactcat taatatcagt tagttgtggg
     1921 ttattaaatt acgcagtatt actttctgta aggtgaatcc aagtcgtcgg cgattcgtgt
     1981 acgacttgcc gacgactcgt catagagtca tcgacgactc acttaagact atcagtaact
     2041 tatgacgact tgactctaga gtcgaatcgt cattttggtg tttttttgac cttggcgact
     2101 ctagagtcaa gtcacgagtc gaatcgccgg tgaatcgtgt ggtgagtcgt ccggcgactt
     2161 gccataacgt cattgccggc cgtcgacgaa ccccattcct gagtgtgctg aaaaccggtt
     2221 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
     2281 ttcggatagg aagaacgccg gaaacaggac ttaccctggc tgaatggcgt gaattttcgc
     2341 gctatgcact tgcgtgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc
     2401 gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcggaaacg ccctgaggcc
     2461 gttttagcgg tgcgtacaat catgccttta tgttaaatga cagatgcaaa aaagccccga
     2521 aggggcctga aggttttcct gctaagggac gaaacgggcc gcctcccggc gacctgttcg
     2581 gcagctccct ctgcgaccat cttatttggt cagttcagcc gtcatgtgga cacggttatt
     2641 gaactgagcg ctggtgattt tgtatgacgc accttgttca gcggcctgtg cggcaatttt
     2701 tgcttcagca cgatccaggg tggaggcggt agcgctgacg ctctgagcaa aggcaccgaa
     2761 agacatcatg gatagagcgg caactgcaac gaaagttttg atggatttca tggtcgtatt
     2821 ccttaagtca ttttgttctg tatggggcgt tgttgccctg atgtgataaa tgataggacg
     2881 accatgaact gattaaaatc gaaacaattt gcggatatta ttcaaaaaaa tagaacaaaa
     2941 attgagcgca tcaaaaccag gtgtgagact ctgatttagg ggcaggatga atagcctcga
     3001 ggggtcgcac aattattagg cgacaattac accgaccgct gccgtcaggc tgagggtatc
     3061 agtgaaagct gccgtgcgac gattgccaat gcatcaaggt gatgaaaaca ggaggggatt
     3121 tattttttaa atgaaaacaa ctatctatgt ttttttctat tgtagtgaaa tcatggtgaa
     3181 tcataagaac agatgcattt tataaaacaa ctcagaagtt gagttgtttt ataaaatgca
     3241 tcttgccatt taaattcaga tggttactta tggtttctgt actcatggtg ctccatatga
     3301 atcaaagcat tgcattgaac cggtggtgct gaattgcgtt attggttttg ttatattgtt
     3361 tacatcgtag cgactgaccg gatgagattg agcgctaccc agcaggacaa atcttgttcc
     3421 tgtgttcgcg caaggcgaac ctaagcaaaa gctagtggtg tccggaacga ttaccaaaga
     3481 aacagaaaaa aggcaccttt caggtgcttt tttttcatct tttagtcgcc agaggcttcc
     3541 cacaaaatac cctaccgcat aaccctacaa agccaattcg ccctagtaag gacggaggcc
     3601 cgtaccctcg atcttcagga taggcactct cgataaccat gttcaaatct tgtaggtgtt
     3661 tctcatcacg cgaggcgcgt cttatttcaa aaaataagtg atatggcccc tctggatcat
     3721 caatcgtaat cgagtaggcc cagtttctct ttgctgatgt ttgatacacc ttttctgctg
     3781 gatcgcatag acggttgata atctcaggta atgccaaaga taatctatat ctaattgggc
     3841 aaaacgttct cccattcccg ctatggtcgt aaattatctg ttctcctgct gggtgataaa
     3901 aagcttcata accttttgta aaacaatgat ttgtgaacct aacgtgtact tttaatacct
     3961 ttttagccat atcgctattt acgtgcataa tgaatggctc aaggtgagtg aaatcgaact
     4021 cttctccaga tatgtctaac gtcgggaaat agctagccat gaattactcc catttagcac
     4081 caacctcctg catacggccc actaatgcat tgtatctttc atctttttcc ttaccttcgt
     4141 ataactcctt aatgacagtc agtgggttaa gcaaaatcac atattgaaac tcacctgatg
     4201 tgccaggttt tgtagttatg aaacctaaat ctcttagttt tctcatcctc cctgtccatg
     4261 tggttactgc tcgctgtcca ctaaaaccag cttcataagc aaatccatct ttatctctta
     4321 tttccacaaa gccctcatca aataccctgc accaaagagc cagatatgtt tgggacaatg
     4381 gcagtcccgt tcctccgaga taatcgagaa ttcgactgat atatgtcagc gttcttggca
     4441 cagttgtata cccgtcatgc tcatggcgat tccatacctt gagttgttca agttcgtctc
     4501 cccatagttc ttctaacaag gttttccttt ttgctgaaag agttcttctt cctgacttct
     4561 tcgctctttc tgagccacta ttgcttactg ttggggggga cgctatcggt agtggcagtg
     4621 gtaacttcgc cattatattc tccaaaatca tttaacatac ggttatttta agtgtttttt
     4681 cttttaaaac aatgagttat gttgtgtttt gcgtgtggtt gtgatgttgc ctttcaacgt
     4741 ataggtatct tgtgagtatg tatttattac aactcaatct gtgagttgta ataaatacag
     4801 catatggtg
//
