LOCUS       NZ_CP069908             8911 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1313 plasmid unnamed7.
ACCESSION   NZ_CP069908
VERSION     NZ_CP069908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357455
            Assembly: GCF_016903395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1345.58x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:17:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,468
            CDSs (with protein)               :: 5,468
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 77 of 165
            Pseudo Genes (incomplete)         :: 94 of 165
            Pseudo Genes (internal stop)      :: 28 of 165
            Pseudo Genes (multiple problems)  :: 29 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069908.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1313"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1313"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..1502)
                     /gene="traD"
                     /locus_tag="I6K43_RS28505"
                     /old_locus_tag="I6K43_28510"
     CDS             complement(<1..1502)
                     /gene="traD"
                     /locus_tag="I6K43_RS28505"
                     /old_locus_tag="I6K43_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="WP_204752778.1"
                     /translation="MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLML
                     MYRLSWQTFVNGCVYWWCTTLGPMRDIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWC
                     GEQLWTSFIFAAVVSLVICIVTFFVASWVLGRQGKQQSEDENTGGRQLSDKPKEVARQM
                     KRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVRARGDMAIIYDRS
                     CEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLSLPDFDNISNTLIPMGTKEDPFWQGS
                     GRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISI
                     RAVLTNYVKAIRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVIS
                     MWLSIAIRGLLAMGENRNRRVWIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYA
                     QLEDIYGVKPAATLFDVMNTRAFFRSPSKEIAEFAAGEIGEK"
                     /name="traD"
     gene            complement(1574..1834)
                     /locus_tag="I6K43_RS28510"
                     /old_locus_tag="I6K43_28515"
     CDS             complement(1574..1834)
                     /locus_tag="I6K43_RS28510"
                     /old_locus_tag="I6K43_28515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020803850.1"
                     /translation="MKNLIITITGIDAGDDSLPCHLYPTETCDTVSFNTSPGLNTETVR
                     AVLYSLFEDACNVDYSQEDGSGSFIAIDSPPNPGCYEYSLK"
                     /name="hypothetical protein"
     gene            complement(1858..2526)
                     /locus_tag="I6K43_RS28820"
     CDS             complement(1858..2526)
                     /locus_tag="I6K43_RS28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020803846.1"
                     /translation="MEETHELTSTDLYSPRVAYESYVSSKTDPSFFSYDVDLLAVSGAV
                     IIFGLLLFYAFSRYAVSSHIKNKCSTLPAGPDKKPDVQSEPDYFHLTLCVEGAADADDS
                     VLSSLASRVVREAENLYIPGINRRDSTDGEFAWSVQTKFPSDKLCGSRLFPETGWFESP
                     NVIERPMKMLCVCLQYTAETSPATLYSLVDEAGRQVEKSYLKGGHYHGSEGYTFCMSQF
                     "
                     /name="hypothetical protein"
     gene            complement(2709..3236)
                     /locus_tag="I6K43_RS28520"
                     /old_locus_tag="I6K43_28525"
     CDS             complement(2709..3236)
                     /locus_tag="I6K43_RS28520"
                     /old_locus_tag="I6K43_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020803849.1"
                     /translation="MMNNKTLIKEVDELKDVLSKGDAEIPSFWVCMWPGLCLVIWNIFC
                     ALISVRSEYYDVKYIFWVLIFPGAVGLILLLGVANARSLFLSVPKSFREKSVIYRFFSK
                     KLLAYAFAYMMIIMALAIYNRFFYDSPFPYAFMVLLTTIFFGFVMNLDFGRYQLSLLTS
                     VLSTLKGNVVEN"
                     /name="hypothetical protein"
     gene            complement(3242..6091)
                     /gene="traG"
                     /locus_tag="I6K43_RS28525"
                     /old_locus_tag="I6K43_28530"
     CDS             complement(3242..6091)
                     /gene="traG"
                     /locus_tag="I6K43_RS28525"
                     /old_locus_tag="I6K43_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="WP_032744228.1"
                     /translation="MTPLEIYTIAGGDWLRGNLNAIAAFMGTATWSSIEKMCIAFSVLI
                     VAVSWVKKHNIMDLLGWVFSLTLVSMLVVIRTPVQIIDYSNVAQVYKVDNVPIGLAIPA
                     SLSTRVGNALVQSYEMIFSLPDSVTYSKTGMLFGGNLVAKSTDFVSQNPEITTLFSDYV
                     QNCVMGDIFLNGKYTLEELMNSSDPYTLVFSNPSPLRGVFDKNNKFQTCLEASRDLKSA
                     LALDSQTGGKTWSYYVRQLFGGKPNPDLLFSQMIGDSYSYFYSSGQSAGQIIRQNVTMN
                     ALRNGIQSYASRSGDTASLVNIANTTSLEKQRLAQATMGHQALRSLPMMQTVIMGIMIG
                     MFPIMIMAAMFNMMTLQVLKGYMFALIWLQSWPLLYAILNSAMAYYARQNGVPVVLSEL
                     SQVQLKNSDIATTAGYISMMIPPLSWAMIKSMGAGFSSAYSHFASSGLSSTSQASSSVV
                     DGNYSFANMQMENVSGYSWGTNSSTSFGQMSRQLGNGGTETQTGDGTTIWDARSSKLPV
                     DINVSRQLASANQQMARESDVQAQSALHGYNSSVTSAWNSLKQFGTNMGTSASTTSGAD
                     NTESSQDSMARSKMWNAVVSNAKANNISNEESFNQLMEDSSKRAQSFDAHVSGKLSSGD
                     QLFGKLGKWGTGLSAEVGAKGSAGWTHNSGDTDSIGSSGRQSHDSRHDTSSQAAKDFKE
                     ASDYFTSRKTSTSGNRTDNNASSRVDQFAASLSSAKNSYDQYTSSRTRSHEYSEMASRT
                     ESMSGQMNENLTQQFAHFVQQRSPQNAEVILTNTSSPEVAAQREALAREFVKAQVEPKV
                     DGAYQQGRESIGQNMSGVSGGSDRGSVMADYGHNAGNIEAMTRDAGIKDNVGQKVDGMI
                     AQNNQQHQETQQGIERDGNEIRKQNSDLEKHHNTEGNKFENEYNERASVVKSLPGADSP
                     RELEAKAAKIQKDYIDGKR"
                     /name="traG"
     gene            complement(6091..7470)
                     /gene="traH"
                     /locus_tag="I6K43_RS28530"
                     /old_locus_tag="I6K43_28535"
     CDS             complement(6091..7470)
                     /gene="traH"
                     /locus_tag="I6K43_RS28530"
                     /old_locus_tag="I6K43_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="WP_162492049.1"
                     /translation="MEDRMRIKLLSLFCGAALGLSFSAAADVNGDMNQFFNKLGFASNT
                     SQPQVWQGQAAGYASGGSLYARTQVKTIQLVSMTLPDINAGCGGIDAYLGSFSFINSEQ
                     LQRFGKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIVGG
                     LFPRTQVAQQKVCQDIAGESNIFADWAASRQGCSVGGQMDKVQDKASDKDKERVMKNIN
                     IMWDALSKNRLFDGNKELKEFVMTLTGTLIFGENSEITPLPARTTDQDLIKAMMEGGTA
                     KIYHCNDSDKCLKVVADATVTITSSKALKSQISALLSSIQNKAVADEKLTDQEKGFISS
                     TTIPVFKYLVDPQMLGVSNSLIYQLTDYIGYDILLQYIQELIQQARAMISTGNYPQSTM
                     DMIMENLNQASVQIAAFQSRVQVQQDALLVVDRQMSYMRQQVSARMMTRYQNNYHFGGS
                     L"
                     /name="traH"
     gene            complement(7448..7891)
                     /gene="trbF"
                     /locus_tag="I6K43_RS28535"
                     /old_locus_tag="I6K43_28540"
     CDS             complement(7448..7891)
                     /gene="trbF"
                     /locus_tag="I6K43_RS28535"
                     /old_locus_tag="I6K43_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="WP_020804675.1"
                     /translation="MNKYIRSTGLYAFLFPASLKAPGQTAAEKIEKLKPEFIHRERRLE
                     IYLELFIVFLTAGALLFWIMRFLFNLCVADWIASGDLRVKDLWNIMMYAIPYALMAVGV
                     GFFVAGVTLAIRNFFSYHLKTLFILRNDRVKKNAVRNGGQDAN"
                     /name="trbF"
     gene            complement(7937..8494)
                     /gene="trbB"
                     /locus_tag="I6K43_RS28540"
                     /old_locus_tag="I6K43_28545"
     CDS             complement(7937..8494)
                     /gene="trbB"
                     /locus_tag="I6K43_RS28540"
                     /old_locus_tag="I6K43_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     thiol-disulfide isomerase TrbB"
                     /protein_id="WP_020804677.1"
                     /translation="MKSSLKNIAKTPLAALALSLALAGLAQAGTLDEVKSLWDPRGISG
                     TETASPQSDTPQAVSGASQPRWLRLSNGKQVDLRDWKVVLFMQGHCPYCHKFDPVLKQL
                     AGQYGFSVFSYTIDGQGDDAFPEALPAPPDVMQTFFPNIPVATPTTFLVNVNTLAAYPI
                     LQGATDAQGFMARVDTVFQMMH"
                     /name="trbB"
     gene            complement(8466..8705)
                     /gene="traQ"
                     /locus_tag="I6K43_RS28545"
                     /old_locus_tag="I6K43_28550"
     CDS             complement(8466..8705)
                     /gene="traQ"
                     /locus_tag="I6K43_RS28545"
                     /old_locus_tag="I6K43_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="WP_023316407.1"
                     /translation="MRKIRFPDLDITGMWVLAVGVFFHLIARLVRKQPELAVQAGEIFG
                     LGMVVFGGYRILNALIAEAEKEEKACEKQPEEHR"
                     /name="traQ"
     Typing          complement(3249..6082)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="84.477"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6095..7449)
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="95.354"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tctttctcac cgatttcgcc ggcggcaaac tccgcaatct ctttactcgg ggagcggaag
       61 aatgcccggg tgttcatgac atcaaagagc gtggcggcgg gtttcacgcc gtagatatct
      121 tccagctggg catacgactg aataccaaag acataacagc ccccgaactt acgggcttcc
      181 gggaggattt cgaccaggtc cggcagtttg tgcagcgtgg gaagctcgtc agcgaaaatc
      241 cacacgcgcc ggttgcggtt ctcccccatc gccagcaggc cgcggatggc aatcgacagc
      301 cacatcgaga taaccggctt cagggaagca tgggtgtctg cgttggagga gatgaacagc
      361 cagccgttgg gctggtcttc acgcacaccg cgcatccagt cgcggatagt gaacggctcg
      421 ccgttcttct caatgccctg caggtagcgg atggccttga cgtagttggt cagcaccgca
      481 cggatactga tggcggtttt ctcaatcttc tcttccacca ggtttgcggc cggggtgttt
      541 tgcagatagg cgcgcagttt gtcaattttg atggacagca tggtgtcgac cagcttctcg
      601 tagctgcgat ccttgtcttc acgcatcagg tacgcgcctt cagcaaagat ggtgcgccct
      661 gatccctgcc agaacgggtc ttctttggtc cccatcggga taagggtatt cgatatgttg
      721 tcgaaatccg gcagcgacag acactctttc cacaaatccc aggcggcaca gcgcgagtct
      781 agcgggttga ggattttatc gagcgacggg tcgtaatagc ttttgacgaa ctcacaggac
      841 cggtcgtaga tgatggccat atcgccccgg gcgcggacgt agttcagcag acgccgtatc
      901 acttcagatt taccggagcc cacagtcccg tggagacaga agttctggat ttcagagttc
      961 agcagtatcg gcaggtcgcc aattttaata tccgaagcca taccatcacg cttcatctgg
     1021 cgggccactt ctttgggctt gtcggacagc tggcgtccgc cggtattctc atcctcgctc
     1081 tgctgtttcc cctgccggcc cagtacccag ctggcaacaa agaacgtcac gatacagatg
     1141 acgagagaaa ccacggcggc gaaaataaaa ctggtccaca actgctcacc gcaccagatg
     1201 gtgtatttat cggccaggat ttgttctgag gtatattcca gcgactggcc gtaatactga
     1261 atggtgtaga cgggctggga gcgaataata tcgcgcatgg gaccgagcgt cgtacaccac
     1321 cagtacacgc agccgttcac aaaggtctgc cagctgagtc ggtacatcag catcagcccg
     1381 cacagcaccc agaataaaat aaacagcaca taaaatataa tattggcaat ctgaccaaac
     1441 atgcggaaac gcatattggc aatctgaccg ccctgcgtca tgtctttggc attaaaactc
     1501 atggcaataa tccgtcagta aaccagtgag ggggataaat taaatcaaca gaaatacgtc
     1561 atcgcaggaa atgttatttc agtgaatact catagcatcc tggattcggg ggagaatcaa
     1621 ttgcaataaa cgaccccgaa ccatcttcct gggaataatc aacattacag gcatcttcaa
     1681 acagagaata aagtaccgct ctgacagtct ctgtattaag ccccggagaa gtattgaaac
     1741 tcaccgtatc gcaggtctct gtcggataaa gatggcaggg caaagaatcg tcacctgcat
     1801 caatacctgt tattgtgata atgaggtttt tcatcgtgtt gaccttaaca ctgttattca
     1861 gaactgactc atgcagaagg tatagccctc agagccatga taatgtccgc ctttaaggta
     1921 cgatttctcc acctgccgcc cggcttcatc gaccagtgaa tacagtgtgg ctggcgatgt
     1981 ttcagcggtg tattgcaggc agacgcacag catcttcatg ggacgttcaa tgacattcgg
     2041 actctcaaac cagcccgtct ccggaaaaag ccgggaacca cacagcttgt cagaaggaaa
     2101 ttttgtctgt acagaccagg caaactcacc gtcagtgctg tcgcggcgat tgatgccggg
     2161 gatataaaga ttctccgctt cgcggacaac acgagaagca agagaagaca ggacggaatc
     2221 atcagcgtct gctgcccctt ccacacataa ggtgagatga aaatagtctg gctcgctctg
     2281 cacgtcaggc tttttatcgg gacccgcagg caaagtacta catttatttt tgatgtgcga
     2341 agatactgca tatcgtgaga aagcgtaaaa caataacaaa ccgaaaataa taacagcgcc
     2401 actaactgct aacaaatcca catcataact gaaaaatgac gggtctgttt ttgaagaaac
     2461 gtatgattcg taagctacac gcggcgaata cagatcggtc gatgtgagtt catgtgtttc
     2521 ttccataaat aacctctgaa tgaataaaga tgaaataatt ccgccgatgg cggcaacgct
     2581 tcacaggcgc acaaataaat tgcgcacatg ttcaacgttt ttgtataaac tatttaaggg
     2641 tatatagaac gatgaaggca gcaagttgca ctctccgccc cgcaactaat aaaaatcacc
     2701 atataaaatc aattttccac cacattacct ttgagagtgc ttaatactga tgtcagcagt
     2761 gacaattgat atcttccaaa atctaaattc ataacaaaac caaaaaaaat cgttgtcaaa
     2821 agaaccatga atgcataagg aaatggggag tcataaaaaa atctattata aatagccagt
     2881 gccatgatta tcatcatgta tgcaaaagca taagcaagaa gtttttttga aaaaaaacga
     2941 tatattactg acttttccct gaaggacttt ggaactgata aaaatagaga acgagcgttc
     3001 gccaccccga gcagaagaat cagacccaca gcaccaggaa atatcaaaac ccagaatatg
     3061 tatttgacat cataatactc tgatcgcaca ctaattaatg cgcaaaaaat attccatatt
     3121 accagacaaa gaccgggcca catgcatacc caaaaagacg gtatttcggc atcgcccttt
     3181 gaaagcacat cttttaactc atccacttct ttaataagcg ttttattatt catcatcatt
     3241 ttcacctttt cccatcaatg taatcctttt gaattttggc tgcctttgcc tctagctcac
     3301 gtggtgagtc agctccgggt aaggatttca ctacgctggc tctttcatta tattcatttt
     3361 cgaacttatt tccctctgta ttatgatgtt tttcgagatc agaattctgt tttctaatct
     3421 cattgccatc acgctctatc ccctgctggg tttcttgatg ctgttgatta ttctgcgcta
     3481 tcatcccatc cactttctga cccacattat ccttaatccc cgcatctcgt gtcatagctt
     3541 cgatattgcc ggcattatgt ccgtagtcgg ccattaccga accacggtca ctaccacctg
     3601 atacaccgga catgttctga ccgatgcttt cgcggccctg ttgataagca ccatcaactt
     3661 ttggttcaac ctgcgctttg acgaattcac gcgccagtgc ctcacgttgc gcagcgactt
     3721 ctggtgaact tgtgttggtc agaatgactt ctgcattctg cggagaacgc tgttggacaa
     3781 agtgagcaaa ctgctgagtc aggttctcgt tcatctggcc gctcatgctc tctgtacggg
     3841 atgccatctc actatattca tggctacgag tccggctgct tgtgtactgg tcatagctgt
     3901 ttttcgcgct cgacagcgat gccgcaaact ggtcaacgcg tgagctggca ttgttatctg
     3961 tcctattacc cgatgtacta gttttacggc tggtaaagta atccgatgct tccttgaagt
     4021 cttttgccgc ctgactgctg gtatcatgac ggctgtcatg tgactgccta cctgaagatc
     4081 cgatactatc cgtatctcct gagttatgag tccaacctgc actgccttta gcaccgactt
     4141 cagctgatag tccagtaccc catttaccca gcttaccgaa tagctgatct cccgaactga
     4201 gtttacctga tacgtgcgca tcgaatgact gagctctttt ggaagaatct tccatcaact
     4261 gattgaatga ctcttcattg ctgatgttat tggccttcgc attagaaaca accgcattcc
     4321 acattttact tctggccatt gaatcctgtg aagactcagt attatcggcc ccactcgtcg
     4381 tcgatgcact ggtacccata tttgtgccaa actgcttcag gctgttccag gcacttgtca
     4441 cgctgctgtt atagccatgc agggcactct gtgcctgcac atctgactca cgtgccatct
     4501 gttggttcgc gctggccagt tgccggctga cattgatatc aaccggtagc ttagaactgc
     4561 gcgcatccca gatagtcgtc ccgtccccgg tctgcgtctc cgtgccaccg ttccccagct
     4621 gccgcgacat ctgaccgaag ctggtcgaac tgttggtacc ccagctgtag ccgctgacgt
     4681 tctccatttg catattggca aaagagtaat tgccgtccac gacgctggat gaggcctggg
     4741 acgtcgaact gagtcccgag gaggcaaagt ggctgtaagc actggagaac cctgccccca
     4801 tactttttat catcgcccag ctgagcggcg gtatcatcat cgagatatac ccggcggtgg
     4861 tcgcaatatc tgagtttttc agctgcacct gagagagttc agacagcacc accggcacac
     4921 cgttctgtct ggcgtaatac gccattgcgc tgttcaggat ggcatacagc agtggccagg
     4981 actgcagcca tataagggca aacatgtatc ctttcaggac ctgcaacgtc atcatattga
     5041 acattgctgc cataatcatg atcgggaaca tcccaatcat gatgcccatg atgaccgtct
     5101 gcatcatcgg cagtgagcgc agtgcctgat gccccatcgt ggcctgggcc agtcgttgtt
     5161 tttccagcga cgtggtgttg gcgatgttca ccaggcttgc tgtgtcgccg ctgcgggacg
     5221 cgtagctctg gatgccgtta cgcagcgcat tcatggtgac attctggcgg ataatctgcc
     5281 cggcactctg gcctgagctg tagaagtagc tgtaactgtc gccaatcatc tggctgaaca
     5341 gcaggtccgg attgggtttg ccgccaaaca actggcgtac ataataactc caggttttcc
     5401 cgccggtctg agagtcaagc gctaacgctg acttcaaatc acgtgaagcc tccagacagg
     5461 tctggaactt gttgttttta tcgaataccc ctctcagcgg gctgggattc gaaaacacca
     5521 gcgtgtacgg gtcagacgag ttcatcagct cttcaagggt gtatttgcca ttgagaaaga
     5581 tgtcacccat cacgcagttc tgcacgtagt cggagaacag cgtggtgatt tcggggttct
     5641 gcgaaacaaa atccgtgctt ttcgccacca ggtttccgcc aaacagcatg ccggtcttac
     5701 tgtaggtgac actgtcgggc agggagaaaa tcatctcgta gctctgcacc agggcattgc
     5761 cgacgcgcgt actcagcgac gccgggatgg ccagaccaat cggcacatta tccactttgt
     5821 agacctgcgc cacgttgctg tagtcgatta tctgcacagg cgtgcggatg acgaccagca
     5881 tcgagaccag ggtcagggag aacacccagc cgaggaggtc catgatgttg tgttttttca
     5941 cccagctgac ggcgacaatc aacaccgaaa acgcgatgca cattttctca atagacgacc
     6001 aggtggcggt ccccatgaac gccgcgatgg cattgagatt accccgcagc cagtcgcctc
     6061 cggcgatggt gtagatttca agtggcgtca tcagaggctc cctccgaaat gatagttatt
     6121 ctggtagcgg gtcatcatgc gggcggagac ctgctggcgc atgtaactca tttgtctgtc
     6181 caccacgagc aaggcgtcct gctggacctg aacacgggac tggaaagcgg caatctgcac
     6241 actcgcctgg ttgaggtttt ccatgatcat atccattgtc gactggggat aattaccggt
     6301 tgagatcatc gcccgggcct gctggataag ttcctgaatg tactgcagca ggatgtcgta
     6361 gccgatatag tctgtcagct ggtaaataag tgagttagac accccgagca tctgcgggtc
     6421 gaccaggtat ttgaacaccg ggatggtggt gctggagata aaccctttct cctgatccgt
     6481 cagtttttca tcagccactg ctttattctg gatactgctt aacaaagcgg aaatctggct
     6541 tttcagcgcc ttactggagg tgatggtcac ggtcgcatca gcaacgacct taagacactt
     6601 atccgaatca ttgcagtggt agattttagc cgtgccgccc tccatcatgg ctttaatcag
     6661 gtcctggtca gtggtgcgtg ctggcagcgg cgtgatctca ctgttctcac caaaaatcag
     6721 cgtcccggtc agggtcatca caaactcctt cagctcctta ttgccgtcaa agagccggtt
     6781 tttggacagg gcatcccaca taatgttgat gttcttcatc acacgctcct tgtccttatc
     6841 gctggcttta tcctgcactt tgtccatctg cccccccacc gaacagccct gacgggaagc
     6901 cgcccagtcc gcaaaaatgt tgctctcgcc ggcaatgtcc tggcagacct tttgctgcgc
     6961 cacctgggtc cgtggaaaca gaccgcccac aatgccctga gcggcctggc aggagctgag
     7021 gttcatgctg ttgatgtcgc tggccatctt ctgcaggaag tctttggcag ttttgatctc
     7081 cggaaccgtg gtctgcaggg caaggtcaaa gaagtagccc gcggcgttgg acataatttg
     7141 tttgccgaag cgctgcagct gctcgctgtt gataaatgag aacgacccca ggtacgcatc
     7201 gatgccgccg cagccggcgt taatatccgg cagggtcatg ctgaccagct ggatcgtttt
     7261 cacctgcgtt ctggcataca gcgaaccgcc ggaggcgtaa cccgccgcct gtccctgcca
     7321 gacctgcggc tggctggtat tggacgcaaa gccaagctta ttgaagaatt ggttcatgtc
     7381 gccattcacg tcggcggcgg cactaaatga caggccaagc gcggcaccgc agaagagtga
     7441 cagaagttta attcgcatcc tgtcctccat tacgcacagc atttttttta actctgtcat
     7501 ttctcagaat aaaaagggtc ttaaggtgat aactgaaaaa attgcggatc gctaatgtca
     7561 ctccggcaac aaagaacccc accccaacag ccatcagcgc ataagggatg gcgtacatca
     7621 taatattcca caggtctttt actcgcaggt caccgctggc tatccagtca gccacgcaga
     7681 gattaaataa aaatctcatt atccaaaaca gcaaagcacc tgcagtgaga aacacaatga
     7741 ataattccag atagatttcc agacgccttt cccggtggat aaattccggc ttcagttttt
     7801 ctattttttc tgctgctgtc tgtccgggtg cttttaaact ggctggaaat aaaaacgcat
     7861 acagtcccgt cgaacgtata tatttgttca tggctatttt ccttaatcac ttgctaagac
     7921 cggatataac agagactcag tgcatcatct gaaataccgt atccactctg gccataaagc
     7981 cctgtgcatc cgtggcgccc tgcaggatcg ggtacgctgc cagggtgttc acgttgacca
     8041 gaaacgtggt cggggtggcc accgggatat tggggaaaaa ggtctgcatc acgtcaggag
     8101 gcgccggcag cgcctcagga aacgcgtcat caccctgtcc gtcaatggtg taagagaaca
     8161 cggagaagcc atactgtccg gccagctgct tcagtaccgg gtcaaactta tgacagtagg
     8221 gacagtgtcc ctgcatgaac agcaccactt tccagtcgcg caggtccacc tgctttccgt
     8281 tgctgagtcg taaccagcgt ggctgagatg ccccgctcac cgcctgggga gtgtcgctct
     8341 gcggggaagc cgtttcagta ccggagattc cccgcggatc ccagagcgac ttcacctcat
     8401 cgagcgtgcc cgcctgtgcc agtccggcca gagcaaggga aagagccagc gccgccagcg
     8461 gcgtcttagc gatgttcttc aggctgcttt tcacaagctt tctcctcttt ttcggcttca
     8521 gcaatcaggg cgttaagaat gcggtatccc ccgaacacca ccattcccag cccgaagatt
     8581 tccccggcct ggacggccag ctcaggctgc tttctgacaa gccgggctat caggtggaaa
     8641 aacacgccca cggccagcac ccacatcccg gtgatatcta aatccggaaa acgaatcttt
     8701 cgcatatatc ctcatctact ttcttagttc atattcataa tatggttttc gtgtgcatgt
     8761 gaatttatta acaaaagccc atgagacatt cacaaaaata actttcactg aagcctcaac
     8821 actatcgaca accacgcaag caatggcatt ccagccataa tacaaactct gaatatcacc
     8881 ttactagcct gtcgggtata aatggcccga t
//
