LOCUS       NZ_CP069905             3557 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1313 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP069905
VERSION     NZ_CP069905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357455
            Assembly: GCF_016903395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1345.58x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:17:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,468
            CDSs (with protein)               :: 5,468
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 77 of 165
            Pseudo Genes (incomplete)         :: 94 of 165
            Pseudo Genes (internal stop)      :: 28 of 165
            Pseudo Genes (multiple problems)  :: 29 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069905.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1313"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1313"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(3480..3557,1..420)
                     /locus_tag="I6K43_RS02700"
                     /old_locus_tag="I6K43_02700"
     CDS             join(3480..3557,1..420)
                     /locus_tag="I6K43_RS02700"
                     /old_locus_tag="I6K43_02700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            427..642
                     /locus_tag="I6K43_RS02705"
                     /old_locus_tag="I6K43_02705"
     CDS             427..642
                     /locus_tag="I6K43_RS02705"
                     /old_locus_tag="I6K43_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1073..1285
                     /locus_tag="I6K43_RS02710"
                     /old_locus_tag="I6K43_02710"
     CDS             1073..1285
                     /locus_tag="I6K43_RS02710"
                     /old_locus_tag="I6K43_02710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(1707..1841)
                     /locus_tag="I6K43_RS28930"
                     /old_locus_tag="I6K43_02715"
     CDS             complement(1707..1841)
                     /locus_tag="I6K43_RS28930"
                     /old_locus_tag="I6K43_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     gene            2461..2802
                     /locus_tag="I6K43_RS02720"
                     /old_locus_tag="I6K43_02720"
     CDS             2461..2802
                     /locus_tag="I6K43_RS02720"
                     /old_locus_tag="I6K43_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2204..2376
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="4.31e-79"
     Typing          complement(1733..2279)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          4..758
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.3999999999999999e-160"
     Typing          2793..3556
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.608"
                     /coverage="99.0"
                     /evalue="7e-170"
ORIGIN
        1 aaagccgcat tcagcaagca cgagaacgtt gtcaaatcgg aactgaacgc aagcgcgaag
       61 agaatcaacg acgccatcag cgcccacgaa caggggatga acgcggcgat gcagtcgaac
      121 cggctgagcg tgctgcgaat ggtcgggcgg acgtggctga ccatcaccat ggtgtcaggg
      181 ctgctgttcg ccagcctgag cggggtgctg tggtaccagg ggaaccggat agcggcgaac
      241 ctggcggaaa tcaggcagca gagagatacg ctgtcgaaac tgcacatgca gacgtggggc
      301 gtgacctatc tggagaaccg caacggacgg tttctggtgc tgccggaggg gatgaaagcc
      361 gaaacgggcc ggacggtgga caacaagacg cggaacgcgg tgaagctggt acgggagtag
      421 agcagaatga cggaactgga aaaacaattg ctgagcgcgt tagagcagct acagcaggac
      481 tactcgagaa ggctggacga gtgggagagc gccttcgagg aatggcggaa gatgtctggt
      541 cttatgcaac gggagaacgc ggcgctgagc gagcgcgtgt cgggcttgag caggcaggtg
      601 cagagtttga gcgagcagct gcgccggtta tcgacaggct gaatgagatt gagcaacagc
      661 gatacgggca aacaacggaa aaggaaccag agaagacagc agatctggta ccggaacggc
      721 aacgcacatt atctcatggt gacagggagt atggagactg ataaacgccc gtctcaggtg
      781 gcctgagcgt gacggggatc atggccattt tctgcgattt cagcacacag agagtctttt
      841 ttaaccgaaa tcaccggcta tgcagtgatg tgaataagtg atgcatatgc gtaaaataaa
      901 tcaggtgcgt ttctggtggt tttcggtgta cttgccacca ccttttgccc gtcagccatc
      961 agaaacgccc tgagtgcctc cggcgcggtg cgggtaagag atgttatggt aaataagagt
     1021 gtctgttgga tattttaaat ctgtttttct attaaattta tgggggtagg aaatggggga
     1081 ttttgagcgt acctacggag ctggtgctga ttttgatcgg ataattgatg agaataacag
     1141 cgaatatttt cgagatgagc gccaaaaagc taaaaattcg aaaaaagaat tctcttcttt
     1201 tcaagatgct ttggtttggg cgaaagctaa tcctggtaaa acgatagtaa gatctccaga
     1261 cggtgtggga tttattgaaa aatgaaagag tggattcact gtaaaacggc agtcattttt
     1321 tggacaacac tggctatata cgtactcata tgtttcacag tgtcgtatgt tggagtctat
     1381 gttacgtaca ttgcagggcc tattttgtta gttacaggaa ttcttgcatg gcttacgaaa
     1441 cctagtagta attcaaataa atctaatatg tgactgggta agacgaactt catccaacca
     1501 taaaacccgc ttttttaaac ggatcttctt gagatcattt agggagaatc gtaatctctt
     1561 agctctgtaa acgaaaaaac cacccggcaa ggtggctttt tcgaaggttc gcaagagttg
     1621 aggctctttt gaaccgcggt aactggcttg gaggagcaca gtcaccaaat tagtcctttc
     1681 agtgtagcct tatcgcaccc gaaatttcaa gactctcctc ctctaaacct gttaccagtg
     1741 gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg actcaagacg atagttaccg
     1801 gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     1861 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     1921 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccaggggg
     1981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     2041 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcaacgccg tggctttatc
     2101 gcttccctgt taagtttctt cctggcatct tccaggaaat ttccgccccg ttcgtaagcc
     2161 atttccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     2221 tatatcctgt atcacatatt ctgctgacgc accggtgccg cctttttctc cttccacatg
     2281 aagcacttca ctgacaccct catccgtgcc aacatagtaa gccagtatac actccgctag
     2341 cgctacgtga ctggttcagg gctgcgcccc gaaaccccac tgacgcgcct gcggcttgtc
     2401 caaccccgcg ccgaccggga aaggctttcc cgtccgtcac ggcgttatca ggaggcgatt
     2461 ctggcagata aacgcaacaa gatgctcacg atgtgggtga cggaagacga acaccggcgg
     2521 ttgcttgagc ggtgcgacgg aaaacaactg gcggcgtgga tgcgccagac ctgccttgat
     2581 gagaaaccga cacgcgccgg caggcttccc tcgatttcgc ccgcgctgct gcgtcagctt
     2641 gccggcatgg gcaataacct gaaccagatc gcccggaaag tgaacgccgg tggtgccggt
     2701 catgaccggg tgcagatcgt ggcagcgctg atggcgatcg atgccggtct ggaacggttg
     2761 cggcatgccg tactggaaaa agggccggac gatgattgtt aaattccatc cccggggtcg
     2821 cggcgggggc gccggtcctg tggattatct tctgggtaaa gaccggcagc gtgaaggtgc
     2881 cagcgtcctg caggggaagc cggaagaagt ccgggagctg attgatgcgt cgccgtatgt
     2941 gaaaaaatat acctccgggg tgctgtcatt cgcggaggcg gacctgccgc tgggtcagcg
     3001 tgaaaaactg atggccagct ttgagcgggt tctgatgccc ggactcgata aagaccagta
     3061 cagcattctg tgggtggaac acgcagacaa gggtcgtctt gagctgaatt ttctgatccc
     3121 caataccgaa ttactgacgg ggaaacgcct ccagccgtat tacgatcgtg ccgaccggcc
     3181 gcgcatcgat gcgtggcaga cagtggtcaa cggcaggctg gggctacatg atccgaacga
     3241 cccggagaac cggcgggcgc tggtcacgcc ttcaggactg ccagaaacga agcaggaagc
     3301 cgcagagacg attacacggg gtttgctgat gttagcctca tcaggagagc tgaaaacgcg
     3361 tgatgacgtc acaggggcgc tcacagcggc cggttttgag gtggtcagaa ccacaaaaaa
     3421 cagtatcagc attgccgacc cggacggggg gcgaaatatc cgactgaggg gagccattta
     3481 tgagcagtct tttaacggcc tcgagggact tcgaggagaa atcgaaagca cagcagcagc
     3541 ttaccgaaga gaggctg
//
