LOCUS       NZ_CP069901             3223 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1313 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP069901
VERSION     NZ_CP069901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357455
            Assembly: GCF_016903395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1345.58x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:17:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,468
            CDSs (with protein)               :: 5,468
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 77 of 165
            Pseudo Genes (incomplete)         :: 94 of 165
            Pseudo Genes (internal stop)      :: 28 of 165
            Pseudo Genes (multiple problems)  :: 29 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1313"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1313"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            97..582
                     /locus_tag="I6K43_RS00005"
                     /old_locus_tag="I6K43_00005"
     CDS             97..582
                     /locus_tag="I6K43_RS00005"
                     /old_locus_tag="I6K43_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            586..801
                     /locus_tag="I6K43_RS00010"
                     /old_locus_tag="I6K43_00010"
     CDS             586..801
                     /locus_tag="I6K43_RS00010"
                     /old_locus_tag="I6K43_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            969..1301
                     /locus_tag="I6K43_RS00015"
                     /old_locus_tag="I6K43_00015"
     CDS             969..1301
                     /locus_tag="I6K43_RS00015"
                     /old_locus_tag="I6K43_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            2319..2642
                     /locus_tag="I6K43_RS00020"
                     /old_locus_tag="I6K43_00020"
     CDS             2319..2642
                     /locus_tag="I6K43_RS00020"
                     /old_locus_tag="I6K43_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             970..1301
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2032..2223
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1569..2049)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..899
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.701"
                     /coverage="99.0"
                     /evalue="6.94e-175"
     Typing          2606..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.718"
                     /coverage="95.0"
                     /evalue="6.19e-121"
ORIGIN
        1 aaagcgcagg ttttgaggtg gtgcgcacca caaaaagcag catcagcatt gccgacccgg
       61 acggggggcg aaacatccga ctgaagggag ccatctatga acagtctttt aacgctggcg
      121 aaggacttag agcagaaatc gaaagcgcag cagcagacta ccggcgagat gctgaaagcc
      181 gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcga gaagagaatc
      241 agcgccgcca tcctcgacca cgaccggaag ctgtcctcag ccatgagcca gcgcacgaaa
      301 gggatgctgc gcatggtcag ccagacgtgg ctgaccatcg ttctggtctc cgcgctgctg
      361 atagcgtcga gcgcgggcat tctgtggtgg caggggcagc agatactcga gaactatacg
      421 accatccggg agcagaagag cacgcaggcc atgctgtcag agaggaacag cggcgtacag
      481 ctctcgacct gcggcgagca gagacgccgc tgcgtgaggg tgaacccgga agcgggacag
      541 ttcggagagg actcgagctg gatgatactg gcggggaaat agcacatgac ggagctggaa
      601 aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag gctggacgag
      661 tgggagaacg ccttcgcgga atggcggacg atgtctggtc ttattcaacg ggagaacgcg
      721 gcgctgaacg agcgcgtcac ggtcttgagc aggcaggtgc agagtttgag cgagcagctg
      781 cgccggttgt cgaaaggctg aacgcgatag aaactcaccg gcagcaggag cgggcggtgc
      841 agcaccagaa agcgctggag ctggaacgat cgcagtggca gcatcatggt ccttcgctat
      901 gataagccgg gttctattaa ttagttgtta tgtaaagata tttaaatttt tcaacatgag
      961 gtatttttat gaatacatat attttcctgg gaggggctat tgtggctgaa gtcataggca
     1021 ccatctttat gaaatattca gaaggattta cacgcctgtg gccgtctgta ggaactgcaa
     1081 taagttattg cgctgccttt tatctgcttt cccaaacatt ggaaaatata cctaccggta
     1141 ttgcttacgc aatttggtct ggtgcgggaa ttgtactaat aagcctagct ggttggttgg
     1201 ttagcggtca aaaacttgat tggcccgcaa ttgcaggaat gttccttatt tgcgttggag
     1261 ttttggttat taatattttt tcaaaatctt ctgcgcatta actttttacc gaagcatatt
     1321 ggccaaagca aaaggatttc cttgagatcc tttctttctg cgcgtaatct ttttccctgt
     1381 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaattcaga gctaccaact
     1441 ctttgttccg atggtaactg gctttcacgg agcgcagata cctaaactgt cctttcagtg
     1501 tagccgcact caggctttca cttcaagaac tctgtaggca ttctctacaa tctctgctcc
     1561 gtgaacccag ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt ccgggttgga
     1621 ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg gttcgtgcat
     1681 acagtccatc ctggagcgaa ctgcctaccc ggaaccgagt ttcaggcgtg gaatgagaaa
     1741 ccgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaatgc gggggagcac
     1801 gagggagcca ccagggggaa acgcctggta tctttaagcc gcatcgggtt tcgccaccgc
     1861 tgatttgagc gtcattttcg tgatactcgt caggggggcg gagcctatgg aaaaacggca
     1921 actccgcatc cttttcttgg cctttcctgc ttcgagcggc atttgcttat cccgtaatct
     1981 gtgagctggt aacgcccgcc gcagtctcac ggcaggagcg tagcgaccga gtgagcgagg
     2041 aggcgtcatt tcatcaccac ctgcattttg cacttacgcc cggcagccgc atttttcccc
     2101 tgtcacatga agcactttca taaattgcca ctccgtcata acatgtttag ccagtataca
     2161 ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaacccgcta aaaccactgc
     2221 cgcgcctgcg gcttgtccaa caccgtgccg accgggaaaa gatttcccgt ccgtcccggc
     2281 gttatcagga ggccggattg gcagacaaac gcagcaaaat gctcaccatg tgggtgactg
     2341 aggacgagca ccggcgtctg ctggagcgct gcgagggtaa gcaactcgcg gcctggatgc
     2401 ggcagacgtg cctggacgag aagcccgcac gcgccggcaa acttccgtcg atctcgccgg
     2461 cgctgcttcg tcagcttgcc ggcatgggga acaaccttaa ccagattgcc cgccaggtta
     2521 acgccggtgg tggaagcgga cacgaccgcg tgcagattgt cgccgcgctg atggccatcg
     2581 atgccggact cgagcggttg cggcatgccg tactggaaaa gggtgctgat gatgatcgtt
     2641 aagtttcatc ctcgggggcg cggcggcggc ggcgggccgg tcgattatct gctgggtaaa
     2701 gatcgccagc gcgacggcgc cagcgttctg caggggaagc cggatgaagt ccgggagctt
     2761 atcgacgcct cgccctacgc caaaaagtac acttccggcg ttctgtcttt tgccgaacag
     2821 gatttaccgc ccggccagcg cgaaaagctg atggcgagtt tcgagcgggt tctgatgccc
     2881 ggactcgata aagaccagta cagcgtgctg tgggttgagc accgggacaa ggggcggctg
     2941 gagctgaact tcctgatccc gaacacggag cttctgaccg gcaagcggct acagccgtat
     3001 tacgaccgcg ccgaccgtcc gcgcatcgat gcctggcaga ccatagtgaa cggcaggctg
     3061 gggctgcatg acccgaacgc gccggagaac cggcgggtgc tggtctcgcc ttccgcgctg
     3121 ccggaagcga agcaggaagc cgcccaggcg attacgagcg gcctgcttgc ccttgcctca
     3181 tccggggagc tgaaaacgcg tcaggacgtc actgaggcgc tgg
//
