LOCUS       NZ_CP069900             1041 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1286 plasmid unnamed7.
ACCESSION   NZ_CP069900
VERSION     NZ_CP069900.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357428
            Assembly: GCF_016903375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1520.67x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,949
            Genes (coding)                    :: 4,749
            CDSs (with protein)               :: 4,749
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 79 of 200
            Pseudo Genes (incomplete)         :: 130 of 200
            Pseudo Genes (internal stop)      :: 52 of 200
            Pseudo Genes (multiple problems)  :: 57 of 200
            Pseudo Genes (short protein)      :: 5 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069900.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1286"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1286"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(108..701)
                     /locus_tag="I6K16_RS25035"
                     /old_locus_tag="I6K16_25035"
     CDS             complement(108..701)
                     /locus_tag="I6K16_RS25035"
                     /old_locus_tag="I6K16_25035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012537770.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="hypothetical protein"
ORIGIN
        1 tcggttttca ctgggtaaaa catctctttt tgcggtatct gcgtcagaat cgcgttcagt
       61 gcgttttagc ggtgcgcgta atgcgacgtt atggtaaatg catttcctca taggccgatt
      121 atttcataga tgtttgactc tctatcttct tttggttgca gatgtatcag gcgactacag
      181 tacagtacat ctaagccgaa cctaatctgg tttggctgga cacctgtata gcttctcagt
      241 gtttcatgtg tcaccttaac agtattattt gactgataac gaaggcttat aattgttaaa
      301 tatagtttta atgccacaaa tggggccatc cccctgttcg atatggtgtt tagttttttc
      361 ctgatagtat ctactggtac ttttgcccac ctatcatcat ttggctgtac tgttaattca
      421 taggagttaa caaacatctc tctatctatc tttataagac catctttctc aagtttggct
      481 actcctttca ctatcatggg gcggcttagt ccagtcagtc tttccattcc ggtaaatgat
      541 actgaggctg ttcttgaatg gaaatcaatc aagacggata gagctaacaa gcatttcatt
      601 gatgcaatgc cgccccctat ctcatttgaa cgaacattgt ttacaagcca tgagcttcgg
      661 gcccaccagt ttggaagcct gccaaatttt ctaccgctca tttttacccc attttcggtc
      721 aaggagagat ataaatagct ttattatcat atacatgaaa accattgcac ctaaagatac
      781 aacagaaacc aaaacctccg gctttgcgga agttagcgtt accagcaaat tgttgagggt
      841 ttccaattgg tcctccagtc gcatactaag ttgttgtgtt ttaactttat atacgcttat
      901 tagaataaat ctagataaag caatatttgg tggttgcttg ttttcccata taaaacatga
      961 tgttatcttg tttttctcgt tttttaactg gtaaatgtaa atttgataat gctggtttaa
     1021 tcggcgtttt tagttatttt t
//
