LOCUS       NZ_CP069899             1171 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1286 plasmid unnamed6.
ACCESSION   NZ_CP069899
VERSION     NZ_CP069899.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357428
            Assembly: GCF_016903375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1520.67x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,949
            Genes (coding)                    :: 4,749
            CDSs (with protein)               :: 4,749
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 79 of 200
            Pseudo Genes (incomplete)         :: 130 of 200
            Pseudo Genes (internal stop)      :: 52 of 200
            Pseudo Genes (multiple problems)  :: 57 of 200
            Pseudo Genes (short protein)      :: 5 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069899.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1286"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1286"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            383..>1171
                     /locus_tag="I6K16_RS25030"
                     /old_locus_tag="I6K16_25030"
     CDS             383..>1171
                     /locus_tag="I6K16_RS25030"
                     /old_locus_tag="I6K16_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204669894.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSY"
                     /name="hypothetical protein"
ORIGIN
        1 ggatggtgaa accatgaaaa atgacgtgtt cagtggatta aggcgggcta atgtggcctg
       61 tagcatctgg ttgcataggt attcatgcgg ttaaaatata tcaggtgcga tcgcggcagt
      121 ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcac cgaacgcgtt
      181 ttggcggtgc gtacaatgcg acgttatggt aaatggagcg gattttcagg aaggagtgat
      241 actaacaact cgaacactgt gcaaataatg atttttttaa aatatcggta acgataataa
      301 attatttcgt tttacgccgg taataaatat aatgtagttt cagtatataa aaaaatatat
      361 gaggtgtctt gtgggagctt tcatgaatct tcaacaacgt ataaataaac taccacaatt
      421 atcatccagt ttttcatttg gaaaagatat tgataatatt cattcattta tctttaacga
      481 aacttctaaa gataaaattg aagatttact aagaaaatgg gtgtcaggca atcagccctg
      541 tgtatttggt aaattggcaa gcaagaaaat taaagggctt gatttccatt tatctattgt
      601 aaatagcccc caactatata atgatgatgg tcatttgttt gattttttgc gtaatgaaag
      661 agttcgtttt aaagaacgcg caagaagagg agaggtcagt gcgcatctca tttattttat
      721 tcatccacaa cttgcctttg ctcgtccatc agaagagtta gtggacatac aaaaatatat
      781 ttgttcgctg catatgccag agtgctatcc aataaaagag gatgttattt atactgaaag
      841 cgtaccattt caggataaag atgggctaaa aatatataag gctggtgtta atgtttttta
      901 ctcttctgct catagaacaa gaaatcatga tagaagaata ccagggggaa tacttatttc
      961 tgtaaatgcc ccggggcatt ttatgaggct ggcgatagaa aaaggtttct ataaagatca
     1021 agaacaagcg ttagcggata ttcgtaacat gactattcag tctgttggta agggtgggta
     1081 ttcacatccg gaagggattt ctacaacatg gcattcagaa tctaatttgg atcgttttgg
     1141 atgcccagtg cacaccggta atagttctta t
//
