LOCUS       NZ_CP069897             2892 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1286 plasmid unnamed4.
ACCESSION   NZ_CP069897
VERSION     NZ_CP069897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357428
            Assembly: GCF_016903375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1520.67x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:01:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,075
            CDSs (total)                      :: 4,949
            Genes (coding)                    :: 4,749
            CDSs (with protein)               :: 4,749
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 79 of 200
            Pseudo Genes (incomplete)         :: 130 of 200
            Pseudo Genes (internal stop)      :: 52 of 200
            Pseudo Genes (multiple problems)  :: 57 of 200
            Pseudo Genes (short protein)      :: 5 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1286"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1286"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            925..1134
                     /locus_tag="I6K16_RS25000"
                     /old_locus_tag="I6K16_25000"
     CDS             925..1134
                     /locus_tag="I6K16_RS25000"
                     /old_locus_tag="I6K16_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(1200..1733)
                     /locus_tag="I6K16_RS25005"
                     /old_locus_tag="I6K16_25005"
     CDS             complement(1200..1733)
                     /locus_tag="I6K16_RS25005"
                     /old_locus_tag="I6K16_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1736..2662)
                     /locus_tag="I6K16_RS25010"
                     /old_locus_tag="I6K16_25010"
     CDS             complement(1736..2662)
                     /locus_tag="I6K16_RS25010"
                     /old_locus_tag="I6K16_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            complement(2667..>2892)
                     /locus_tag="I6K16_RS25015"
                     /old_locus_tag="I6K16_25015"
     CDS             complement(2667..>2892)
                     /locus_tag="I6K16_RS25015"
                     /old_locus_tag="I6K16_25015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204669892.1"
                     /translation="FLMNPIRFFSHSMLLMRMFVMLPGVFLRCVVVVQYIACGRRVQGV
                     SSVLIRKIRRKRTYMFLSFVEKQCAPAEG"
                     /name="hypothetical protein"
     Typing          complement(1740..2662)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaaaacaag tgtagttttc taagtaaatt agattcgaca accaattagt ttatccacat
       61 cgcattccac tcttgctaca ccaatcacat ttcttgatat gtaatagcag aaaaaataaa
      121 accagacacc aatagaccaa aaaacaaaca ttcaacaaga aacattttga acaatatcac
      181 aaaactgtca caaccatcat cagactattg aaaaaaactg gttgctgttt tataaaagcg
      241 actgtaacaa cctccactct atagttccat tagtttaaag tgtcacacaa actgagcatc
      301 catttgtttt ccttactttt gcccctgtat tccaaggggt ttttttataa cttaaagatt
      361 agcacaccac acaaaaacaa taaaaaaaaa cgacaagtta tacaataaaa ccagtactat
      421 ccatacagct aattacatat aaatactatt aaaccatctg gaaattaatg cggttccggt
      481 accatatgtc agggataatg tgcaaatgga ggtatcggaa ctgcatttca gttcattatt
      541 aattaatgac aaaaaaaacc tctgcgagcg cagagggggt aaggaatgtt cccggtcata
      601 atatgtcgat agtgtttata tacatacaga acaagaacaa caaccagcca actaataaat
      661 attttatttt ttagagctga aaccttaaag ctcacttcca ttccatacat tgttataaac
      721 cttatgttga aaactgaaag taagtacggc agacttatta agttgcgtgc agtcttttca
      781 cgttatacaa acaagaacac ctgcgaagtt atttctcctt acatatcccc ctatgccaac
      841 gggggatttt ttctggttca ctaacgcata aataatagtg acaccctatc attcacaaac
      901 cgtgataata taaggattat catcatggca aaattaggtg aaaacacccc acatattatc
      961 gatgctactg tagattttat ggcatcaagc caggcatttc gtgagtatct gaaaaaactt
     1021 ccccctgata acacaatacc gccagctctt actgatgagc acgtaccgtt atatatacga
     1081 aggctggaat actatcgtct gctttatcga ccaaagcaga cagaatcata ttgaaaaaaa
     1141 cggtgaacgg ttcgcgaaat cctttcgcgg gtcgtgaggc gttggacaag cgaagcgcgt
     1201 cagtttttcc ggctcttccc cgtgacaggt ttctccacca gtttatcttc cagtgtcaca
     1261 actttctgat acaccgccgc tgtggcgttg tagttcctgt cgagtattgt tttcattccc
     1321 catccgagca cacagaacat aaccagaaaa gccactgaca ccccacccag ccatttccag
     1381 ctataccctt taagggtgtt gtattcctgc acgatattgc tgtgaattgt cgcaatatcc
     1441 ctgttcaggg catccgcgtt tgccttcatg tttgcgactg actcttccag ccgctgaaaa
     1501 tcctggctga tgcgctcacc tatcagtcct gccacgtcca gcttctgcac ctcatttctg
     1561 acttcatccc tgacctgttt tgccacaccg tcagaaatat cccgatatcc gcgaagagtt
     1621 tcccttgcca cagccagaga atcagcaaca gctttctgtt gctccattgc ggtatgttgc
     1681 agatgctcca ccgataacag gagcgtgagt aatttttctt ctgtgctggc catgttcaga
     1741 atcccataga aaatccacgt gacggtgatt taacccgttc aggtgcagtt cggttctgtc
     1801 tgctcactct gtctctcatg tcaaagccat gctcccgcgc aacatcatgt tgcagccttc
     1861 tgaccagttc atgttgctta accggagtgt ccagccattc cctgatgtca tgcccgaccg
     1921 ttcgcgcctg ttcaagatgg cgggaatagt ccggttcaga ccgtttttgc atgatgtcat
     1981 gacgctgggc atcatcaagc cgtccggcag cttcaaaacc cagatgcact tcgggacgtc
     2041 gttcaatgcc ctgttctttc agcgtccggc agtcaatgaa gcccctttca aagccatgct
     2101 ttctcatatg ttcgttgtgc ttcacctccc agcgttttcg caattcccgc aactgctctt
     2161 tttgctgagt gagagtcagg cttccggaat cctttttcgc acctccccgc tcgggatacc
     2221 tggcgttata gcgccggaaa tactgttccg gtgtacgttc aatgccatca ttcacacgct
     2281 gactcatcat gatatgcgca tgaggctgtt cgccgccgtc aatgctcgct ttcggattgt
     2341 gaatggcaaa actccaggcg tgccgttcgc cagcttcctg acgtacaaaa tcctgcacca
     2401 gttcaaggcg ctgttccggt gtcagttcac ggggtaatgc aatttcaagt tccctatagg
     2461 tcgaaccgtt cgcacgttca aactggtcag ccgcctgcca gaaatgggaa ggattatccc
     2521 tggcccattc cggcatgttt ccgtgtgcgg aatattccaa atcctgacgg tcgcggtatt
     2581 tctctttgcg ctcgatgtaa tcggcgtggt ttgcagcctg ccccttgcca ccaaatttaa
     2641 cgctgaggtg ataggttgcc acggtttcag ccctctgcag gagcgcactg tttctcgacg
     2701 aaggagagaa acatataagt gcgcttcctt ctaattttac gaattagcac ggaagatacc
     2761 ccctgcaccc gccgaccgca agcgatatac tgcacgacca caacacaccg caaaaaaaca
     2821 ccagggagca tcacgaacat gcgcatcagc aacatcgaat ggctgaagaa acggatcgga
     2881 ttcatcagga ag
//
